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5110739 Process for selective fermentation with mutants of pediococcus halophilus
PATENT ABSTRACTS
5110725 OPTICAL PROBE FOR THE CYTOCHROME P-450 CHOLESTEROL CLEAVAGE
SIDE CHAIN ENZYME
Babetta L Marrone, Daniel J Simpson, Clifford Unkefer, Thomas W Whaley assigned to The United States of America as represented by the United States Department of Energy An optical probe enables the study of enzyme activity by absorbance spectroscopy or by sensitive fluorescence methods. In particular, the probe provides the ability to monitor the activity of cytochrome P-g50scc enzyme, the rate limiting enzyme for steroid biosynthesis. Located on the inner mitochondriai membrane, P-450scc catalyzes the conversion of cholesterol to pregnenolone and isocapraldehyde by sequential oxidations of the cholesterol side chain. The flUorogenic probe includes a cholesterol-like steroid linked to a chromophore through a linking group. The chromophore is selected to have little optical response when linked to the steroid substrate and an enhanced optical response when cleaved from the substrate and linking group. Thus, a fluorescent anion that can be optically detected is generated by the sidechain cleavage reaction during steroidogenesis.
5110726 IMMUNOASSAY FOR ANTIBODIES BINDING PLATELETS
481
platelet protein, The partially purified platelet antigens resulting from these manipulations are then preferably atfixed to a solid matrix. The solid matrix preferably comprises nitrocellulose paper, polystyrene or latex but may be any solid matrix suitable for the abstraction from a biological sample and/or assay of antibodies binding to the affixed purified platelet antigens.
5110739 PROCESS FOR SELECTIVE FERMENTATION WITH MUTANTS OF PEDIOCOCCUS HALOPHILUS Keietsu Abe, Kinji Uchida, Noda, Japan assigned to Kikkoman Corporation A process is provided for selective fermentation with microorganisms belonging to the Genus Pediococcus and having reduced or no phosphoenolypyruvate dependent sugar:phosphotransferase system, The microorganism is capable of fermenting a medium containing a metaboIite which induces catabolite repression and a metabolite whose catabolism is inhibited by catabolite repression. The metabolite which induces catabolite repression may be selected from glucose and mannose and the metabolite whose catabolism is inhibited by catabolite repression may be selected from arabinose, xylose, galactose, sucrose, maltose, trehalose, lactose, and glycerin. The Pediococcus microorganisms used in this process are mutant strains designated Pediococcus halophilis I-I, having an accession number FERM BP-1303, and Pediococcus halophilis I-2, having an accession number FERM BP-1304.
Daryl Ogden assigned to Board of Regents The University of Texas System The present invention involves a method for producing a substrate useful in a system for the detection of antibodies directed against platelet antigens. This method comprises several steps. A platelet sample of interest is initially treated with an aqueous solution comprising a dialyzable nonionic detergent. This initial treatment is under conditions to solubitize platelet components and produce a platelet lysate. Such conditions may involve treatment of a platelet sample with an aqueous solution comprising nonionic detergent at a concentration between about 0.2% and about 0.5%. Platelet antigens are most preferably solubilized for about 30 rain and at about 0 degrees C. in an aqueous solution comprising about I m g dialyzable nonionic detergent per mg
5110741 AERATION APPARATUS FOR THE CULTURE OF MAMMALIAN CELLS Kiyomot Ohi, Shi Shimizu, Nara, Japan assigned to Toyo Boseki Kabushiki Kaisha An aeration apparatus for the culture of mammalian cells comprises a plurality of bundles composed of thin-walled narrow tubes made of gas permeable material, a first head for introducing a gas to the bundles through a gas connector and a second head for removing gas from the bundles through a gas connector, both the
5110725 OPTICAL PROBE FOR THE CYTOCHROME P-450 CHOLESTEROL CLEAVAGE
SIDE CHAIN ENZYME
Babetta L Marrone, Daniel J Simpson, Clifford Unkefer, Thomas W Whaley assigned to The United States of America as represented by the United States Department of Energy An optical probe enables the study of enzyme activity by absorbance spectroscopy or by sensitive fluorescence methods. In particular, the probe provides the ability to monitor the activity of cytochrome P-g50scc enzyme, the rate limiting enzyme for steroid biosynthesis. Located on the inner mitochondriai membrane, P-450scc catalyzes the conversion of cholesterol to pregnenolone and isocapraldehyde by sequential oxidations of the cholesterol side chain. The flUorogenic probe includes a cholesterol-like steroid linked to a chromophore through a linking group. The chromophore is selected to have little optical response when linked to the steroid substrate and an enhanced optical response when cleaved from the substrate and linking group. Thus, a fluorescent anion that can be optically detected is generated by the sidechain cleavage reaction during steroidogenesis.
5110726 IMMUNOASSAY FOR ANTIBODIES BINDING PLATELETS
481
platelet protein, The partially purified platelet antigens resulting from these manipulations are then preferably atfixed to a solid matrix. The solid matrix preferably comprises nitrocellulose paper, polystyrene or latex but may be any solid matrix suitable for the abstraction from a biological sample and/or assay of antibodies binding to the affixed purified platelet antigens.
5110739 PROCESS FOR SELECTIVE FERMENTATION WITH MUTANTS OF PEDIOCOCCUS HALOPHILUS Keietsu Abe, Kinji Uchida, Noda, Japan assigned to Kikkoman Corporation A process is provided for selective fermentation with microorganisms belonging to the Genus Pediococcus and having reduced or no phosphoenolypyruvate dependent sugar:phosphotransferase system, The microorganism is capable of fermenting a medium containing a metaboIite which induces catabolite repression and a metabolite whose catabolism is inhibited by catabolite repression. The metabolite which induces catabolite repression may be selected from glucose and mannose and the metabolite whose catabolism is inhibited by catabolite repression may be selected from arabinose, xylose, galactose, sucrose, maltose, trehalose, lactose, and glycerin. The Pediococcus microorganisms used in this process are mutant strains designated Pediococcus halophilis I-I, having an accession number FERM BP-1303, and Pediococcus halophilis I-2, having an accession number FERM BP-1304.
Daryl Ogden assigned to Board of Regents The University of Texas System The present invention involves a method for producing a substrate useful in a system for the detection of antibodies directed against platelet antigens. This method comprises several steps. A platelet sample of interest is initially treated with an aqueous solution comprising a dialyzable nonionic detergent. This initial treatment is under conditions to solubitize platelet components and produce a platelet lysate. Such conditions may involve treatment of a platelet sample with an aqueous solution comprising nonionic detergent at a concentration between about 0.2% and about 0.5%. Platelet antigens are most preferably solubilized for about 30 rain and at about 0 degrees C. in an aqueous solution comprising about I m g dialyzable nonionic detergent per mg
5110741 AERATION APPARATUS FOR THE CULTURE OF MAMMALIAN CELLS Kiyomot Ohi, Shi Shimizu, Nara, Japan assigned to Toyo Boseki Kabushiki Kaisha An aeration apparatus for the culture of mammalian cells comprises a plurality of bundles composed of thin-walled narrow tubes made of gas permeable material, a first head for introducing a gas to the bundles through a gas connector and a second head for removing gas from the bundles through a gas connector, both the