- Email: [email protected]
5151511 DNA encoding avian growth hormones
New Patents
v
5151510
5151755
METHOD OF SYNETHESIZING SULFURIZED OLIGONUCLEOTIDE ANALOGS
METHOD POR DETECTING DEFECI~ IN MEASUREMENT MEANS OF BIOCHEMICAL ANALYSIS APPARATUSES
Wojciech J Stec, Bogdan Uznanski, B John Betgot, Bernard L Hirschbein, Karen L Fearon, Lodz, Poland assigned to Appfied Biosystems Inc A method for synthesizing sulfurized oligonucleotide analogs, such as phosphorothioate and phosphorodithioate analogs, is provided that employs a thiophosphorus compound, such as a thiophosphoric, dithiophosphoric, thiophosphinic, or dithiophosphinic acid disulfide or polysulfide, as a sulfurizing agent. The method of the invention may be used to sulfurize any phosphorous(III)-containing intermediate. Preferably, the method is practiced on a commercial DNA synthesizer using phosphoramidite and/or phosphorthioamidite intermediates.
Shunichi Seto, Kanagawa, Japan assigned to Fuji Photo Film Co Ltd Defects in a measurement device are detected in a biochemical analysis apparatus wherein a droplet of liquid sample is applied to an analysis medium containing a reagent, which chemically reacts with a specific constituent in the liquid sample, the analysis medium is then incubated, the optical densities of the analysis medium are determined, and concentration of the specific constituent in the liquid sample is determined from the optical densities of the analysis media thus determined. The method for detecting defects comprises the steps of irradiating light, which has passed through a plurality of interference filters by turns, to a reference density plate, and measuring the amount of light reflected by the reference density plate. Measured values representing the amounts of reflected light, which have thus been measured for the interference filters, are compared with one another. Defects in the measurement device are detected from the results of the comparison,
5151511 DNA ENCODING AVIAN GROWTH HORMONES
5152982
Lawrence Souza, Thomas C Boone assigned to Amgen Inc
COMPOSITIONS AND METHODS FOR FELV VACCINATION
Novel polypeptides possessing biochemical and immunological properties of avian growth hormone are obtained by practice of recombinant DNA procedures. Novel DNA sequences are disclosed which are capable of directing the synthesis of such polypeptides in selected host microorganisms. In a preferred embodiment, a novel polypeptide with the properties of avian growth hormone of chicken species origins is produced as a result of bacterial expression of a novel plasmid, cGH-T21. This plasmid is harbored in transformed E. coli C600 cells deposited as A.T.C.C. 39182. Also disclosed is a DNA sequence capable of directing the synthesis of a novel polypeptide with the properties of avian growth hormone of turkey species origin.
Debora Parkes, Paul Luciw, Gary Van Nest, Dino Dina assigned to Chiron Corporation Novel methods and compositions are provided for vaccinating a host susceptible to viral infection. Particularly, an initial injection is employed of one or more polypeptides associated with the host immune response to the pathogen followed by administration of the pathogen in attenuated form, particularly having a deletion associated with the gene~expressing at least one of said polypeptides. In this manner, hosts may be vaccinated so as to maintain a strong defensive posture against infection from the pathogen. The method is specifically illustrated with feline leukemia virus.
v
5151510
5151755
METHOD OF SYNETHESIZING SULFURIZED OLIGONUCLEOTIDE ANALOGS
METHOD POR DETECTING DEFECI~ IN MEASUREMENT MEANS OF BIOCHEMICAL ANALYSIS APPARATUSES
Wojciech J Stec, Bogdan Uznanski, B John Betgot, Bernard L Hirschbein, Karen L Fearon, Lodz, Poland assigned to Appfied Biosystems Inc A method for synthesizing sulfurized oligonucleotide analogs, such as phosphorothioate and phosphorodithioate analogs, is provided that employs a thiophosphorus compound, such as a thiophosphoric, dithiophosphoric, thiophosphinic, or dithiophosphinic acid disulfide or polysulfide, as a sulfurizing agent. The method of the invention may be used to sulfurize any phosphorous(III)-containing intermediate. Preferably, the method is practiced on a commercial DNA synthesizer using phosphoramidite and/or phosphorthioamidite intermediates.
Shunichi Seto, Kanagawa, Japan assigned to Fuji Photo Film Co Ltd Defects in a measurement device are detected in a biochemical analysis apparatus wherein a droplet of liquid sample is applied to an analysis medium containing a reagent, which chemically reacts with a specific constituent in the liquid sample, the analysis medium is then incubated, the optical densities of the analysis medium are determined, and concentration of the specific constituent in the liquid sample is determined from the optical densities of the analysis media thus determined. The method for detecting defects comprises the steps of irradiating light, which has passed through a plurality of interference filters by turns, to a reference density plate, and measuring the amount of light reflected by the reference density plate. Measured values representing the amounts of reflected light, which have thus been measured for the interference filters, are compared with one another. Defects in the measurement device are detected from the results of the comparison,
5151511 DNA ENCODING AVIAN GROWTH HORMONES
5152982
Lawrence Souza, Thomas C Boone assigned to Amgen Inc
COMPOSITIONS AND METHODS FOR FELV VACCINATION
Novel polypeptides possessing biochemical and immunological properties of avian growth hormone are obtained by practice of recombinant DNA procedures. Novel DNA sequences are disclosed which are capable of directing the synthesis of such polypeptides in selected host microorganisms. In a preferred embodiment, a novel polypeptide with the properties of avian growth hormone of chicken species origins is produced as a result of bacterial expression of a novel plasmid, cGH-T21. This plasmid is harbored in transformed E. coli C600 cells deposited as A.T.C.C. 39182. Also disclosed is a DNA sequence capable of directing the synthesis of a novel polypeptide with the properties of avian growth hormone of turkey species origin.
Debora Parkes, Paul Luciw, Gary Van Nest, Dino Dina assigned to Chiron Corporation Novel methods and compositions are provided for vaccinating a host susceptible to viral infection. Particularly, an initial injection is employed of one or more polypeptides associated with the host immune response to the pathogen followed by administration of the pathogen in attenuated form, particularly having a deletion associated with the gene~expressing at least one of said polypeptides. In this manner, hosts may be vaccinated so as to maintain a strong defensive posture against infection from the pathogen. The method is specifically illustrated with feline leukemia virus.