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5194386 Xanthomonas campestris strain expressing xanthan gum
116
PATENT ABSTRACTS
second fine particles are formed by double or "multiple hybridization reaction of the denatured objective genomic substance added with the first and second single-stranded nucleic acid probes. The aggregations are then digested with a nuclease which cleaves the non-complementary mismatch-containing portion of the double strand of each hybrid permitting the formation of the aggregations, in the vicinity of a mismatch-localized region, but substantially not the completely complementary portion of the double strand of the hybrid. The size of the aggregations in the digested solution is first measured and then the degree of mismatch between the complementary base sequences of the objective genomic substance and the first or second single-stranded nucleic acid probe is measured using the size of the aggregations.
5194375 DNA ENCODING INTERLEUKIN-7 RECEPTORS AND METHODS OF USE Linda S Park, Raymond G Goodwin, Higashiyamato, assigned to Immunex Corporation Mammalian Interleukin-7 receptor proteins, DNAs and expression vectors encoding mammalian IL-7 receptors, and processes for producing mammalian IL-7 receptors as products of recombinant cell culture, are disclosed.
coding sequences. The putative ribosome binding site is preferably properly positioned without the intervening sequences by a crossover linker mutagenesis procedure before the modified foreign gene is introduced into the virus. The putative ribosome binding site preferably comprises at least the final four nucleotides of the sequences ACCTATAAAT immediately upstream of the translation inibiation codon (ATG) of the foreign gene. The system is capable of producing foreign gene proteins (when insect cells are infected with the recombinant virus) at high levels, even in the case of those genes which expressed only at low or intermediate levels in prior recombinant baculovirus systems.
5194381 FELINE INTERFERON AND PROCESS FOR PRODUCTION THEREOF Akira Yanai, Yoshizumi Ueda, Toru Sakurai, Masahiro Satoh, Gloucester, assigned to Toray Industries Inc A recombinant silkworm nuclear polyhedrosis virus containing D N A coding for a protein of feline interferon; the recombinant virus is constructed by cotransfection a recombinant plasmid having a gene coding for a protein of feline interferon and a silkworm nuclear polyhedrosis virus D N A into established silkworm cells, and cloning the desired recombinant virus. The recombinant virus is useful for a massproduction of feline interferon.
5194376 51~3~ BACULOVIRUS EXPRESSION SYSTEM CAPABLE OF PRODUCING FOREIGN GENE PROTEINS AT HIGH LEVELS C Yong Kang, Gloucester, Canada assigned to University of Ottawa A baculovirus expression system capable of producing foreign gene proteins at high levels. The system involves the production of a recombinant baculovirus containing a modified foreign gene between the polyhedrin gene promoter region and the transcription termination signal of the polyhedrin structural gene. The modified foreign gene comprises a putative ribosome binding site immediately upstream of the foreign gene coding sequence, i.e. without any intervening non-
XANTHOMONAS CAMPESTRIS STRAIN EXPRESSING XANTHAN GUM Thomas J Pollock, Linda Thorne, Gloucester, assigned to Shin-Etsu Chemical Co Ltd; ShinEtsu Bio Inc A method of increasing xanthan gum production, comprising culturing a Xanthomonas campestris strain having a xanthan-increasing modification in a culture medium, wherein the modification is selected from the group consisting of (1) a mutation causing rifampicinresistance; (2) a mutation causing bacitracinresistance; or (3) exogenous genetic information controlling the synthesis of xanthan; and
117
PATENT ABSTRACTS separating xanthan from the culture medium, is provided along with specific DNA sequences and Xanthomonas campestris strains showing increased xanthan gum production.
protective 41kD antigen bend from P. falciparum protects Aotus monkeys completely from a P. falciparum infection in model experiments.
5194387
5194592
PROCESS FOR THE I N C O R P O R A T I O N O F DNA MOLECULES INTO MICROORGANISMS WITH METHYL-SPECIFIC RESTRICTION SYSTEMS
MONOCLONAL ANTIBODIES TO NOVEL POLYPEPTIDE DERIVATIVES OF HUMAN GRANULOCYTE COLONY STIMULATING FACTOR
Douglas J MacNeit assigned to Merck & Co Inc There is disclosed a process for the incorporation of DNA into a microorganism that, because of natural restrictions in the organism, normally would prevent or limit the incorporation of the DNA. The process involves the selection of an intermediate host organism that has no restrictions to the incorporation of DNA. The DNA is then transferred into the intermediate host which is readily accomplished because there are no restrictions. The DNA is then isolated from the intermediate host and transferred into the target organism. Using this process the natural restrictions of the host organism are circumvented and DNA is transferred from a source organism to a target organism that normally would not be possible. In this manner desirable characteristics from the source organism can be imparted into the target organism.
5194587 MALARIA-SPECIFIC DNA SEQUENCES, EXPRESSION PRODUCTS THEREOF, AND THE USE THEREOF Bernhard Knapp, Erika Hundt, Burkhard Enders, Han Kupper, Gloucester, assigned to Behringwerke Aktiengesellschaft The invention relates to malaria-specific DNA sequences, to the expression products thereof, and to the use thereof. A combination of three of the expression proteins whose DNA sequences were isolated by screening a lambda gtll gene bank with a monospccific antiserum against the
Hajim Yoshida, Kanagawa, Japan assigned to Kyowa Hakko Kogyo Co Ltd Novel hG-CSF polypeptide derivatives having an amino acid sequence derived from the amino acid sequence of the haman granulocyte colony stimulating factor polypeptide by substitution of at least one amino acid by a different aminoacid and/or deletion of at least one amino acid, recombinant plasmids containing a DNA fragment insert coding for any of these hG-CSF polypeptide derivatives, microorganisms carrying one of such plasmids, and methods of producing the hG-CSF polypeptide derivatives using the microorganisms are described.
51~5~ CHIMERIC GLYCOPROTEINS CONTAINING IMMUNOGENIC SEGMENTS OF THE GLYCOPROTEINS OF HUMAN RESPIRATORY SYNCYTIAL VIRUS Michael W Wathen assigned to The Upjohn Company This invention encompasses DNA compositions encoding novel chimeric glycoproteins which are useful for preparing virus specific immune responses against haman respiratory syncytial virus. The DNA compositions include structural genes coding for the glycoprot¢ins and expression and replication plasmids containing the structural genes. Host cells transformed with the above DNA compositions, vaccines made from the glycoprotcins and methods for protecting humans by inoculation with said vaccines are also part of this invention.
PATENT ABSTRACTS
second fine particles are formed by double or "multiple hybridization reaction of the denatured objective genomic substance added with the first and second single-stranded nucleic acid probes. The aggregations are then digested with a nuclease which cleaves the non-complementary mismatch-containing portion of the double strand of each hybrid permitting the formation of the aggregations, in the vicinity of a mismatch-localized region, but substantially not the completely complementary portion of the double strand of the hybrid. The size of the aggregations in the digested solution is first measured and then the degree of mismatch between the complementary base sequences of the objective genomic substance and the first or second single-stranded nucleic acid probe is measured using the size of the aggregations.
5194375 DNA ENCODING INTERLEUKIN-7 RECEPTORS AND METHODS OF USE Linda S Park, Raymond G Goodwin, Higashiyamato, assigned to Immunex Corporation Mammalian Interleukin-7 receptor proteins, DNAs and expression vectors encoding mammalian IL-7 receptors, and processes for producing mammalian IL-7 receptors as products of recombinant cell culture, are disclosed.
coding sequences. The putative ribosome binding site is preferably properly positioned without the intervening sequences by a crossover linker mutagenesis procedure before the modified foreign gene is introduced into the virus. The putative ribosome binding site preferably comprises at least the final four nucleotides of the sequences ACCTATAAAT immediately upstream of the translation inibiation codon (ATG) of the foreign gene. The system is capable of producing foreign gene proteins (when insect cells are infected with the recombinant virus) at high levels, even in the case of those genes which expressed only at low or intermediate levels in prior recombinant baculovirus systems.
5194381 FELINE INTERFERON AND PROCESS FOR PRODUCTION THEREOF Akira Yanai, Yoshizumi Ueda, Toru Sakurai, Masahiro Satoh, Gloucester, assigned to Toray Industries Inc A recombinant silkworm nuclear polyhedrosis virus containing D N A coding for a protein of feline interferon; the recombinant virus is constructed by cotransfection a recombinant plasmid having a gene coding for a protein of feline interferon and a silkworm nuclear polyhedrosis virus D N A into established silkworm cells, and cloning the desired recombinant virus. The recombinant virus is useful for a massproduction of feline interferon.
5194376 51~3~ BACULOVIRUS EXPRESSION SYSTEM CAPABLE OF PRODUCING FOREIGN GENE PROTEINS AT HIGH LEVELS C Yong Kang, Gloucester, Canada assigned to University of Ottawa A baculovirus expression system capable of producing foreign gene proteins at high levels. The system involves the production of a recombinant baculovirus containing a modified foreign gene between the polyhedrin gene promoter region and the transcription termination signal of the polyhedrin structural gene. The modified foreign gene comprises a putative ribosome binding site immediately upstream of the foreign gene coding sequence, i.e. without any intervening non-
XANTHOMONAS CAMPESTRIS STRAIN EXPRESSING XANTHAN GUM Thomas J Pollock, Linda Thorne, Gloucester, assigned to Shin-Etsu Chemical Co Ltd; ShinEtsu Bio Inc A method of increasing xanthan gum production, comprising culturing a Xanthomonas campestris strain having a xanthan-increasing modification in a culture medium, wherein the modification is selected from the group consisting of (1) a mutation causing rifampicinresistance; (2) a mutation causing bacitracinresistance; or (3) exogenous genetic information controlling the synthesis of xanthan; and
117
PATENT ABSTRACTS separating xanthan from the culture medium, is provided along with specific DNA sequences and Xanthomonas campestris strains showing increased xanthan gum production.
protective 41kD antigen bend from P. falciparum protects Aotus monkeys completely from a P. falciparum infection in model experiments.
5194387
5194592
PROCESS FOR THE I N C O R P O R A T I O N O F DNA MOLECULES INTO MICROORGANISMS WITH METHYL-SPECIFIC RESTRICTION SYSTEMS
MONOCLONAL ANTIBODIES TO NOVEL POLYPEPTIDE DERIVATIVES OF HUMAN GRANULOCYTE COLONY STIMULATING FACTOR
Douglas J MacNeit assigned to Merck & Co Inc There is disclosed a process for the incorporation of DNA into a microorganism that, because of natural restrictions in the organism, normally would prevent or limit the incorporation of the DNA. The process involves the selection of an intermediate host organism that has no restrictions to the incorporation of DNA. The DNA is then transferred into the intermediate host which is readily accomplished because there are no restrictions. The DNA is then isolated from the intermediate host and transferred into the target organism. Using this process the natural restrictions of the host organism are circumvented and DNA is transferred from a source organism to a target organism that normally would not be possible. In this manner desirable characteristics from the source organism can be imparted into the target organism.
5194587 MALARIA-SPECIFIC DNA SEQUENCES, EXPRESSION PRODUCTS THEREOF, AND THE USE THEREOF Bernhard Knapp, Erika Hundt, Burkhard Enders, Han Kupper, Gloucester, assigned to Behringwerke Aktiengesellschaft The invention relates to malaria-specific DNA sequences, to the expression products thereof, and to the use thereof. A combination of three of the expression proteins whose DNA sequences were isolated by screening a lambda gtll gene bank with a monospccific antiserum against the
Hajim Yoshida, Kanagawa, Japan assigned to Kyowa Hakko Kogyo Co Ltd Novel hG-CSF polypeptide derivatives having an amino acid sequence derived from the amino acid sequence of the haman granulocyte colony stimulating factor polypeptide by substitution of at least one amino acid by a different aminoacid and/or deletion of at least one amino acid, recombinant plasmids containing a DNA fragment insert coding for any of these hG-CSF polypeptide derivatives, microorganisms carrying one of such plasmids, and methods of producing the hG-CSF polypeptide derivatives using the microorganisms are described.
51~5~ CHIMERIC GLYCOPROTEINS CONTAINING IMMUNOGENIC SEGMENTS OF THE GLYCOPROTEINS OF HUMAN RESPIRATORY SYNCYTIAL VIRUS Michael W Wathen assigned to The Upjohn Company This invention encompasses DNA compositions encoding novel chimeric glycoproteins which are useful for preparing virus specific immune responses against haman respiratory syncytial virus. The DNA compositions include structural genes coding for the glycoprot¢ins and expression and replication plasmids containing the structural genes. Host cells transformed with the above DNA compositions, vaccines made from the glycoprotcins and methods for protecting humans by inoculation with said vaccines are also part of this invention.