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5209929 Immunization against babesiosis using purified merozoite surface antigens of basesia bigemina
PATENT
ganglioside antibody identified as L612. Both alpha and beta type anti-idiotype antibodies are disclosed which belong to the IgG1 class and contain kappa light chains. Two hybridoma cell lines which produced the monoclonal antiidiotype antibodies are identified. The beta-type anti-idiotype antibodies are useful as an immunization agent to raise antibodies which are immunoreactive with tumors. The alpha-type anti-idiotype antibodies are useful as a probe for use in detecting human monoclonal antibodies bound to biopsied tumor tissues and to identify expression of ganglioside antigens on biopsied human tissues.
5208321 HIV-2 TRANSMEMBRANE GLYCOPROTEIN HOMODIMER ( G P 80) Ara G Hovanessian, Marie-Ann Rey, Anne G Laurent, Bernar Krust, Luc Montagnier, Montrouil, France assigned to Institut Pasteur Characterization of the envelope transmembrane protein of haman immunodeficiency virus type 2 (HIV-2) was carried out using murine polyclonal and monoclonal antibodies or patient sera specific for HIV-2 proteins. A 80Mr glycoprotein (gp80) was produced in HIV-2 infected cells along with three other glycoproteins that were recently reported: the extracellular glycoprotein (gp125), the envelope glycoprotein precursor (gpl40), and the transient dimeric form of gpl40 (gp300). The gp125 and gp80 were detectable after the synthesis of gpl40 and the formation of gp300. Among these four glycoproteins, only gp80 and gp 125 were associated with HIV-2 virions. As the other glycoproteins, gp80 was recognized by all HIV-2 positive sera. A murine polyclonal antibody raised against the purified gp300 recognized all four glycoproteins. On the other hand, a monoclonal antibody raised against a synthetic polypeptide deduced from the sequence of the transmembrane glycoprotein of HIV-2, recognized gpl40, gp300 and gp80; thus indicating that gp80 should be related to the transmembrane protein of the envelope. Dimerization of envelope glycoprotein precursor and the transmembrane glycoprotein was also observed in cells infected with simian immunodeficiency virus (SIV), a virus closely related to HIV-2. Dimerization of the envelope precursors might be essential for thc processing of these glycoprotcins into the mature products, extracellularand transmcmbranc glycoproteins.Fur-
205
ABSTRACTS
thermor¢, the dimeric form of the transmembrane glycoproteins might be important for the optimal structure of the virus and thus for its infectivity.
5209929 IMMUNIZATION AGAINST BABESIOSIS USING PURIFIED MEROZOITE SURFACE ANTIGENS OF BASESIA BIGEMINA Travi McGuire, Terr McElwain, Lane Perryman, William Davis assigned to Washington State University Research Foundation Antigenic surface proteins from the intraerythrocytic merozoite stage of Babesia bigemina have been isolated using cell fusions and monoelonal antibodies produced thereby. Immunization of mammals, such as bovines, with purified isolates induces an immunological response that is effective to reduce pathological effects of babesiosis induced by Babesia bigemina. Diagnostic kits using monocional antibodies and antigenic surface proteins of Babesia bigemina are also disclosed.
5210018 IMMUNOENZYMATIC METHOD IN HOMOGENEOUS PHASE FOR THE DETERMINATION OF ANTI-PLASMODIUM FALCIPARUM-SPOROZOITE ANTIBODIES IN HUMAN BLOOD Carlo Nuzzolo, Adrian Bernardi, Antonello Pessi, Antonio S Verdini, Rome, Italy assigned to Eniricerche S p A An immunoenzymatic method for the detection of anti-Plasmodium falciparum-sporozoite antibodies in a sample of human blood, which operates, in homogeneous phase, and under suitable conditions, with a synthetic polypeptidic antigen (P), a synthetic antigen-enzyme (P-E) conjugate, wherein said antigen is capable of specifically reacting with the antiPlasmodium falciparum-sporozoite antibodies (Ab) possibly present in the sample, and an inert substance capable of quantitatively precipitating the antibody-synthetic antigen-enzyme complex (Ab-P-E). The method, due to its specificity, sensitivity, reproducibility and rapidity, is par-
ganglioside antibody identified as L612. Both alpha and beta type anti-idiotype antibodies are disclosed which belong to the IgG1 class and contain kappa light chains. Two hybridoma cell lines which produced the monoclonal antiidiotype antibodies are identified. The beta-type anti-idiotype antibodies are useful as an immunization agent to raise antibodies which are immunoreactive with tumors. The alpha-type anti-idiotype antibodies are useful as a probe for use in detecting human monoclonal antibodies bound to biopsied tumor tissues and to identify expression of ganglioside antigens on biopsied human tissues.
5208321 HIV-2 TRANSMEMBRANE GLYCOPROTEIN HOMODIMER ( G P 80) Ara G Hovanessian, Marie-Ann Rey, Anne G Laurent, Bernar Krust, Luc Montagnier, Montrouil, France assigned to Institut Pasteur Characterization of the envelope transmembrane protein of haman immunodeficiency virus type 2 (HIV-2) was carried out using murine polyclonal and monoclonal antibodies or patient sera specific for HIV-2 proteins. A 80Mr glycoprotein (gp80) was produced in HIV-2 infected cells along with three other glycoproteins that were recently reported: the extracellular glycoprotein (gp125), the envelope glycoprotein precursor (gpl40), and the transient dimeric form of gpl40 (gp300). The gp125 and gp80 were detectable after the synthesis of gpl40 and the formation of gp300. Among these four glycoproteins, only gp80 and gp 125 were associated with HIV-2 virions. As the other glycoproteins, gp80 was recognized by all HIV-2 positive sera. A murine polyclonal antibody raised against the purified gp300 recognized all four glycoproteins. On the other hand, a monoclonal antibody raised against a synthetic polypeptide deduced from the sequence of the transmembrane glycoprotein of HIV-2, recognized gpl40, gp300 and gp80; thus indicating that gp80 should be related to the transmembrane protein of the envelope. Dimerization of envelope glycoprotein precursor and the transmembrane glycoprotein was also observed in cells infected with simian immunodeficiency virus (SIV), a virus closely related to HIV-2. Dimerization of the envelope precursors might be essential for thc processing of these glycoprotcins into the mature products, extracellularand transmcmbranc glycoproteins.Fur-
205
ABSTRACTS
thermor¢, the dimeric form of the transmembrane glycoproteins might be important for the optimal structure of the virus and thus for its infectivity.
5209929 IMMUNIZATION AGAINST BABESIOSIS USING PURIFIED MEROZOITE SURFACE ANTIGENS OF BASESIA BIGEMINA Travi McGuire, Terr McElwain, Lane Perryman, William Davis assigned to Washington State University Research Foundation Antigenic surface proteins from the intraerythrocytic merozoite stage of Babesia bigemina have been isolated using cell fusions and monoelonal antibodies produced thereby. Immunization of mammals, such as bovines, with purified isolates induces an immunological response that is effective to reduce pathological effects of babesiosis induced by Babesia bigemina. Diagnostic kits using monocional antibodies and antigenic surface proteins of Babesia bigemina are also disclosed.
5210018 IMMUNOENZYMATIC METHOD IN HOMOGENEOUS PHASE FOR THE DETERMINATION OF ANTI-PLASMODIUM FALCIPARUM-SPOROZOITE ANTIBODIES IN HUMAN BLOOD Carlo Nuzzolo, Adrian Bernardi, Antonello Pessi, Antonio S Verdini, Rome, Italy assigned to Eniricerche S p A An immunoenzymatic method for the detection of anti-Plasmodium falciparum-sporozoite antibodies in a sample of human blood, which operates, in homogeneous phase, and under suitable conditions, with a synthetic polypeptidic antigen (P), a synthetic antigen-enzyme (P-E) conjugate, wherein said antigen is capable of specifically reacting with the antiPlasmodium falciparum-sporozoite antibodies (Ab) possibly present in the sample, and an inert substance capable of quantitatively precipitating the antibody-synthetic antigen-enzyme complex (Ab-P-E). The method, due to its specificity, sensitivity, reproducibility and rapidity, is par-