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5213969 Cloned N-methylhydantoinase
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PATENT ABSTRACTS
5213961 ACCURATE QUANTITATION OF RNA AND DNA BY COMPETETITIVE POLYMERASE CHAIN REACTION H Franklin Bunn, Gary Gilliland, Kerry Blanchard, Steven Perrin assigned to Brigham and Women's Hospital A process for quantitating nucleic acids species in a sample is described which comprises coamplification of a competitive template with the sample template wherein the competitive template utilizes primers identical to those utilized by the sample template and wherein the competitive template is distinguishable from the sample template.
5213969 CLONED NMETHYLHYDANTOINASE Gunther Schumacher, Helmut Burtscher, Hans Mollering, Bernried, Federal Republic Of Germany assigned to Boehringer Mannbeim GmbH The invention concerns a DNA which codes for a protein with N-methylhydantoinase activity and which has (1) the nucleic acid sequence shown in FIG. (1), (2) a sequence corresponding to it within the scope of the degeneracy of the genetic code or (3) a sequence which hybridizes with a sequence from (1) or/and (2) under stringent conditions. Furthermore the invention also concerns a recombinant vector which contains a DNA according to the present invention, a cell which is transformed with a vector according to the present invention as well as a process for producing a recombinant protein with NMHase activity. 5213972 FERMENTATION PROCESS FOR THE PRODUCTION OF PYRIMIDINE DEOXYRIBONUCLEOSIDES Russell J McCandliss, David Anderson assigned to Chemgen Corporation DNA coding for at least one enzyme that causes the accumulation of a pyrimidine deoxyribonucleoside is used, in conjunction with meta-
bolic mutations or heterologous DNA coding for metabolic enzymes that also increase pyrimidine deoxyribonucleoside production, to engineer cultured cells to express a pyrimidine deoxyribonucleoside (PdN) in recoverable quantifies, providing a commercially useful fermentation source for PdNs. 5213977 SERINE PROTEASE FROM CYTOTOXIC KILLER CELLS Irving L Weissman, Howard K Gershenfeld assigned to The Board of trustees of the Leiand Stanford Junior University A human serine protease expressed in cytotoxic killer cells, having a mass of about 25.8kD, and having the amino acid residues of the serine protease charge-relay catalytic mechanism conserved is provided. The protease can be produced by recombinant DNA technology. The cDNA is also provided.
5213980 FIBROBLAST GROWTH FACTOR GENE TRANSFORMED HYBRIDOMA AND ENHANCED PRODUCTION OF ANTIBODY Hidekazu Sawada, Keiji Iwamoto, Kazuaki Kitano, Osaka, Japan assigned to Takeda Chemical Industries Ltd Disclosed are (1) a hybridoma carrying a vector for expressing a flbroblast growth factor (FGF) protein gene; (2) a method for producing the hybridoma of (1) which comprises transforming a hybridoma with the vector for expressing the FGF protein gene; and (3) a method for producing a biologically active substance which comprises cultivating in a culture medium the hybridoma obtained by the method of(2) using a hybridoma producing a biologically active substance other than the FGF protein, producing the FGF protein and producing and accumulating the biologically active substance in a culture, and recovering the biologically active substance, whereby the biologically active substance can be efficicntly produced and recovered using thc serum-free medium, which is advantageous for industrial production and very useful for an improvement in the breeding of the hybridoma.
PATENT ABSTRACTS
5213961 ACCURATE QUANTITATION OF RNA AND DNA BY COMPETETITIVE POLYMERASE CHAIN REACTION H Franklin Bunn, Gary Gilliland, Kerry Blanchard, Steven Perrin assigned to Brigham and Women's Hospital A process for quantitating nucleic acids species in a sample is described which comprises coamplification of a competitive template with the sample template wherein the competitive template utilizes primers identical to those utilized by the sample template and wherein the competitive template is distinguishable from the sample template.
5213969 CLONED NMETHYLHYDANTOINASE Gunther Schumacher, Helmut Burtscher, Hans Mollering, Bernried, Federal Republic Of Germany assigned to Boehringer Mannbeim GmbH The invention concerns a DNA which codes for a protein with N-methylhydantoinase activity and which has (1) the nucleic acid sequence shown in FIG. (1), (2) a sequence corresponding to it within the scope of the degeneracy of the genetic code or (3) a sequence which hybridizes with a sequence from (1) or/and (2) under stringent conditions. Furthermore the invention also concerns a recombinant vector which contains a DNA according to the present invention, a cell which is transformed with a vector according to the present invention as well as a process for producing a recombinant protein with NMHase activity. 5213972 FERMENTATION PROCESS FOR THE PRODUCTION OF PYRIMIDINE DEOXYRIBONUCLEOSIDES Russell J McCandliss, David Anderson assigned to Chemgen Corporation DNA coding for at least one enzyme that causes the accumulation of a pyrimidine deoxyribonucleoside is used, in conjunction with meta-
bolic mutations or heterologous DNA coding for metabolic enzymes that also increase pyrimidine deoxyribonucleoside production, to engineer cultured cells to express a pyrimidine deoxyribonucleoside (PdN) in recoverable quantifies, providing a commercially useful fermentation source for PdNs. 5213977 SERINE PROTEASE FROM CYTOTOXIC KILLER CELLS Irving L Weissman, Howard K Gershenfeld assigned to The Board of trustees of the Leiand Stanford Junior University A human serine protease expressed in cytotoxic killer cells, having a mass of about 25.8kD, and having the amino acid residues of the serine protease charge-relay catalytic mechanism conserved is provided. The protease can be produced by recombinant DNA technology. The cDNA is also provided.
5213980 FIBROBLAST GROWTH FACTOR GENE TRANSFORMED HYBRIDOMA AND ENHANCED PRODUCTION OF ANTIBODY Hidekazu Sawada, Keiji Iwamoto, Kazuaki Kitano, Osaka, Japan assigned to Takeda Chemical Industries Ltd Disclosed are (1) a hybridoma carrying a vector for expressing a flbroblast growth factor (FGF) protein gene; (2) a method for producing the hybridoma of (1) which comprises transforming a hybridoma with the vector for expressing the FGF protein gene; and (3) a method for producing a biologically active substance which comprises cultivating in a culture medium the hybridoma obtained by the method of(2) using a hybridoma producing a biologically active substance other than the FGF protein, producing the FGF protein and producing and accumulating the biologically active substance in a culture, and recovering the biologically active substance, whereby the biologically active substance can be efficicntly produced and recovered using thc serum-free medium, which is advantageous for industrial production and very useful for an improvement in the breeding of the hybridoma.