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5278058 Process for the production of lignolytical enzymes by means of Phanerochaete chrysosporium
PATENT ABSTRACTS
583
one or more substances from the group consisting of diaphorase, phenazine methosulphate, phenazine ethosulphate, phenazine phenosulphate and Meldola blue; one or more substances from the group consisting of NAD, NADP, thioNAD, thio-NADP, nicotinamide-purine dinucleotide, nicotinamide-methylpurine dinucleotide, nicotinamide-2-chloro methylpurine dinucleotide; one or more hemolysing substances from the group consisting of phospholipase, hernolysing saponins, and compounds of hydrophilic mono-, di- or trisaccharides and aliphatic hydrocarbons having 10-16 carbon atoms; a redox indicator dye; and optionally mutarotase. A color change brought about by the reaction of the reagent with glucose in the undiluted whole blood is measured by transmission spectrophotometry,
A process for the production of lignolytical enzymes using Phanerochaete chrysosporium which includes placing a culture of the pocket rot fungus Phanerochaete chrysosporium into a closed fermentation vessel which has no stirring mechanism. Pellets of Phanerochaete chrysosporium are produced in the vessel by rotating and slewing the vessel, and enzyme is then harvested. An apparatus for carrying out the process includes a cardanic mount for freely rotatably and slewably suspending the vessel.
5278048
Herb G Bull, Kevin T Chapman assigned to Merck & Co Inc
METHODS FOR DETECTING THE EFFECT OF CELL AFFECTING AGENTS ON LIVING CELLS John W Parce, Harden M McConnell, Gillian M Humphries, Karen M Kercso, John C Owicki, Josef E Kercso assigned to Molecular Devices Corporation Methods are disclosed for detecting the effects of cell affecting agents on living cells. The method steps include providing living cells that are retained in a micro flow chamber. The micro flow chamber is adapted for either continuous or intermittent flow of solutions or suspensions in intimate contact with the cells. The solutions or suspensions, which contain a cell affecting agent, are then flowed in intimate contact with the cells and at least one effect of the cell affecting agent on the cells is measured by an appropriate detecting means, which is operatively associated with the micro flow chamber.
5278058 PROCESS FOR THE PRODUCTION OF LIGNOLYTICAL ENZYMES BY MEANS OF PHANEROCHAETE CHRYSOSPORIUM Hans-Peter Call, D 5132 Palenberg, Federal Republic Of Germana
5278061 AFFINITY CHROMATOGRAPHY MATRIX USEFUL IN PURIFYING INTERLEUKIN-I BETA CONVERTING ENZYME
Affinity chromatography matrices are disclosed which are useful in purifying interleukin-1 beta converting enzyme (ICE) from crude or partially purified cell lysate preparations. The chromatographic matrices comprise a specific ICE inhibitor of Formula I which is attached to an affinity column support by means o f a bifunctional spacer. See Patent for Chemical Structure I
5278080 METHOD FOR MEASURING THE FREE FRACTION OF LIGANDS IN BIOLOGICAL FLUIDS John E Midgley, Christopher Sheehan, Nicos Christofides, Great Missenden, United Kingdom assigned to Amersham International PLC A one-step assay for the free portion of a ligand in a biological sample involves incubating a mixture of the sample with a labelled antibody for the ligand and a ligand analogue which competes with the ligand for binding to the antibody. The assay is characterized by choosing a ligand analogue which has a lower affinity for the antibody than does the ligand. An insolubilised ligand analogue preferably has a binding affinity for the antibody from 0,01 ~o to 10~oof that of the ligand. Ligand/ligand analogue pairs exemplified are T4/T3 (Thyroxine/Triiodothyronine); Testosterone/etiocholanol; and T3/T2.
583
one or more substances from the group consisting of diaphorase, phenazine methosulphate, phenazine ethosulphate, phenazine phenosulphate and Meldola blue; one or more substances from the group consisting of NAD, NADP, thioNAD, thio-NADP, nicotinamide-purine dinucleotide, nicotinamide-methylpurine dinucleotide, nicotinamide-2-chloro methylpurine dinucleotide; one or more hemolysing substances from the group consisting of phospholipase, hernolysing saponins, and compounds of hydrophilic mono-, di- or trisaccharides and aliphatic hydrocarbons having 10-16 carbon atoms; a redox indicator dye; and optionally mutarotase. A color change brought about by the reaction of the reagent with glucose in the undiluted whole blood is measured by transmission spectrophotometry,
A process for the production of lignolytical enzymes using Phanerochaete chrysosporium which includes placing a culture of the pocket rot fungus Phanerochaete chrysosporium into a closed fermentation vessel which has no stirring mechanism. Pellets of Phanerochaete chrysosporium are produced in the vessel by rotating and slewing the vessel, and enzyme is then harvested. An apparatus for carrying out the process includes a cardanic mount for freely rotatably and slewably suspending the vessel.
5278048
Herb G Bull, Kevin T Chapman assigned to Merck & Co Inc
METHODS FOR DETECTING THE EFFECT OF CELL AFFECTING AGENTS ON LIVING CELLS John W Parce, Harden M McConnell, Gillian M Humphries, Karen M Kercso, John C Owicki, Josef E Kercso assigned to Molecular Devices Corporation Methods are disclosed for detecting the effects of cell affecting agents on living cells. The method steps include providing living cells that are retained in a micro flow chamber. The micro flow chamber is adapted for either continuous or intermittent flow of solutions or suspensions in intimate contact with the cells. The solutions or suspensions, which contain a cell affecting agent, are then flowed in intimate contact with the cells and at least one effect of the cell affecting agent on the cells is measured by an appropriate detecting means, which is operatively associated with the micro flow chamber.
5278058 PROCESS FOR THE PRODUCTION OF LIGNOLYTICAL ENZYMES BY MEANS OF PHANEROCHAETE CHRYSOSPORIUM Hans-Peter Call, D 5132 Palenberg, Federal Republic Of Germana
5278061 AFFINITY CHROMATOGRAPHY MATRIX USEFUL IN PURIFYING INTERLEUKIN-I BETA CONVERTING ENZYME
Affinity chromatography matrices are disclosed which are useful in purifying interleukin-1 beta converting enzyme (ICE) from crude or partially purified cell lysate preparations. The chromatographic matrices comprise a specific ICE inhibitor of Formula I which is attached to an affinity column support by means o f a bifunctional spacer. See Patent for Chemical Structure I
5278080 METHOD FOR MEASURING THE FREE FRACTION OF LIGANDS IN BIOLOGICAL FLUIDS John E Midgley, Christopher Sheehan, Nicos Christofides, Great Missenden, United Kingdom assigned to Amersham International PLC A one-step assay for the free portion of a ligand in a biological sample involves incubating a mixture of the sample with a labelled antibody for the ligand and a ligand analogue which competes with the ligand for binding to the antibody. The assay is characterized by choosing a ligand analogue which has a lower affinity for the antibody than does the ligand. An insolubilised ligand analogue preferably has a binding affinity for the antibody from 0,01 ~o to 10~oof that of the ligand. Ligand/ligand analogue pairs exemplified are T4/T3 (Thyroxine/Triiodothyronine); Testosterone/etiocholanol; and T3/T2.