- Email: [email protected]
5352581 Sensitive yeast genetic system for identifying agents causing doublestranded DNA damage
264
PATENT ABSTRACTS
administered to skin in combination with exposure of the skin to UV radiation. The result is an enhanced level of melanin production, i.e., more tanning than achieved by UV radiation alone. The administration of the DNA repair enzymes in liposomes also reduces the level of DNA damage caused by the UV exposure. Accordingly, both the tanning response is increased and the deleterious effect of UV exposure is decreased. The method can be used by the general population as well as by individuals whose skin is susceptible to UVinduced damage.
5352575 PSEUDORABIES VIRUS PROTEIN Erik A Petrovskis, Leonard E Post, James Timmins assigned to The Upjohn Company The present invention provides recombinant DNA molecules comprising a sequence encoding a pseudorabies virus (PRV) glycoprotein selected from the group consisting o f g I , gp50, and gp63, host cells transformed by said recombinant DNA molecule sequences, the gI, l~nv50 and gp63 polypeptides. The present ention also provides subunit vaccines for PRV, methods for protecting animals against PRV infection and methods for distinguishing between infected and vaccinated animals.
5352578 METHOD OF SEPARATING OLIGONUCLEOTIDES FROM A MIXTURE Sudhir Agrawal, Paul Zamecnik assigned to Worcester Foundation for Experimental Biology A method of purifying full length synthetic target olignnucientides from a mixture of oligonucleotides, particularly from a mixture containing truncated or failed sequences is disclosed. The method involves attaching a short nucleotide sequence complementary to the 5' end of a target oligonucleotide to a solid support. The complementarity between the most 5' nucleotides of the target olignnucleotide and the bound oligonucieotide results in hybridization which serves to retain the target oligonuclentide. Truncated or failed sequences lacking 5' sequences complementary to the attached oligonucieotide, fail tc hybridize and therefore are not retained. The method makes it ossible to purify gram quantifies of synthetic eoxyribonuclcic acids or ribonucleic acids and sequences which have modifications, such as on
~
the phosphate backbone. The support-bound nucleotide sec~uences are stable under conditions of purification and therefore can be reused.
5352580 SELECTIVE DETECTION OF MYCOBACTERIA BY NUCLEICACID PROBES DERIVED FROM MYCOBACTERIUM KANSASII Patricia A Spears, Daryl Shank assigned to Becton Dickinson and Company Oligonucleotide probes derived from Mycobacteria K a n ~ i i capable of selectively hybridizing to Mycobucteria nucleic acid are disclosed. The oligonucleotide probe is selected from the group consisting of: (a) an oligonucieotide probe consisting essentially of the DNA sequence given herein as SEQ ID NO:I (MKI4); (b) oligonucleotide probes comprising fragments of MKI4 which retain the capabifity of MK14 of selectively hybridizing to Mycobacteria nucleic acid; (c) ol/gnnucleotide probes which hybridize to MKI4 and are capable of selectively hybridizing to Mycobacteria nucleic acid; and (d) oligonucleotide probes which are complementary to any of the foregoing and are capable of selectively hybridizing to Mycobacteria nucleic acid. The probes are useful in nucleic acid amplification and hybridization assays for genus-specific detection of the mycobacteria, Se~.fic detection of M. kansasii and specific on of the slow-growing mycobacteria. Methods of using the probes to detect and amplify Mycobacteria nucleic acid and kits containing the same are also disclosed.
5352581 SENSITIVE YEAST GENETIC SYSTEM FOR IDENTIFYING AGENTS CAUSING DOUBLESTRANDED DNA DAMAGE Michael A Resnick, Torston Nilsson-Tillgren assigned to United States/National Institutes of Health A sensitive, yeast-based genetic system for identifying agents causing double-strand DNA damage is described. The system comprises a yeast strain containing either chromosomes having divergent (homenlogous) DNA sequences, a single nonhomologous chromosome or a single artificial chromosome with suitable genetic markers so that double-strand damage leading to the loss of such chromosome
PATENT ABSTRACTS due to the inability to undergo recombinational repair with a homolog is detected.
5352587 COMPOSITIONS AND METHODS FOR THE SYNTHESIS OF NATRIURETIC PROTEIN RECEPTOR B AND METHODS OF USE
derivatives of bFGF can act as antagonists and/ or superagonists of the wild type molecule in the angiogenic process. These derivatives, as well as wild type bFGF, may be prepared by the use of strains or E. coil which have been transformed with plasmids carrying nucleotide sequence coding for human and bovine bFGF and their derivatives,
Ming-Sh Chang, David V Goeddel, David G Lowe, Newbury Park, Canada assigned to Genentech Inc Described are the amino acid sequence of natriuretic peptide receptor B (NPRB) and DNA encoding NPRB. Also disclosed are expression vectors and cells transformed to express the NPRB, DNA encoding NPRB and diagnostic and therapeutic uses for the NPRB and the DNA encoding NPRB.
5~25~ STREPTOCOCCAL IMMUNOGLOBULIN A BINDING PROTEIN ENCODED BY EMML2.2
265
5352592 MICROBIOLOGICAL PROCESS FOR THE PRODUCTION OF 5HYDROXYPYRAZINE CARBOXYLIC ACID Andreas Kiener, Visp, Switzerland assigned to Lonza Ltd A microbiological process for the production of 5-hydroxypyraziuec~boxylic acid and/or its salts with microorganisms of the strain Agrobacterium sp. DSM 6336 or descendants thereof or mutants thereof, utilizing 3-cyanopyridine.
Vincent A Fischetti, Debra E Bessen assigned to Rockefeller University The subject invention concerns a novel polynuclentide sequence cloned from emm2.2 gene of a Group A streptococcus, Type II strain which codes for an IgA-binding protein, ML2.2. A process for produdng the protein is given. The invention also concerns the protein in an immunoadsorbent and as a tracer for use in measuring and purifying IgA. Kits an: given comprising the immunoadsorbent and the tracer form of the protein.
5~2~9 DELETION MUTANT OF BASIC FIBROBLAST GROWTH FACTOR AND PRODUCTION THEREOF Laur Bergonzonl, Antonella Isuchi, Paolo Sarmientos, Gilles Cauet, Milan, Italy assigned to Farmitalia Carlo Erba S R L The present invention relates to the production, by recombinant DNA techniques, of derivatives of basic fibroblast growth factor (bFGF). These
5352595 MYOD
REGULATORY
REGION
Stephen J Tapscott, Harold M Weintranb, Theodore D Palmer assigned to Fred Hutchinson Cancer Research Center Isolated DNA or RNA molecules capable o f hybridizing under stringent conditions to the myoD regulatory region, its proximal promoter and distal enhancer regulatory regions, and regulatory elements within the proximal and distal regions for binding basic helix-loop-helix proteins, MyoD, and proteins binding at SPI, API, CAAT, M-CAT, CArG, and MEF sites in DNA. DNA or RNA expression vectors for introducing a geue into a cell under the regulatory control of the myoD regulatory region. Transduced or transfected pre-musele cells, myocytes, or myoblasts. Methods of inducing a muscle phenotype in a non-muscle cell, positively selecting for a cells expressing MyoD, and negatively selecting for cells expressing MyoD.
PATENT ABSTRACTS
administered to skin in combination with exposure of the skin to UV radiation. The result is an enhanced level of melanin production, i.e., more tanning than achieved by UV radiation alone. The administration of the DNA repair enzymes in liposomes also reduces the level of DNA damage caused by the UV exposure. Accordingly, both the tanning response is increased and the deleterious effect of UV exposure is decreased. The method can be used by the general population as well as by individuals whose skin is susceptible to UVinduced damage.
5352575 PSEUDORABIES VIRUS PROTEIN Erik A Petrovskis, Leonard E Post, James Timmins assigned to The Upjohn Company The present invention provides recombinant DNA molecules comprising a sequence encoding a pseudorabies virus (PRV) glycoprotein selected from the group consisting o f g I , gp50, and gp63, host cells transformed by said recombinant DNA molecule sequences, the gI, l~nv50 and gp63 polypeptides. The present ention also provides subunit vaccines for PRV, methods for protecting animals against PRV infection and methods for distinguishing between infected and vaccinated animals.
5352578 METHOD OF SEPARATING OLIGONUCLEOTIDES FROM A MIXTURE Sudhir Agrawal, Paul Zamecnik assigned to Worcester Foundation for Experimental Biology A method of purifying full length synthetic target olignnucientides from a mixture of oligonucleotides, particularly from a mixture containing truncated or failed sequences is disclosed. The method involves attaching a short nucleotide sequence complementary to the 5' end of a target oligonucleotide to a solid support. The complementarity between the most 5' nucleotides of the target olignnucleotide and the bound oligonucieotide results in hybridization which serves to retain the target oligonuclentide. Truncated or failed sequences lacking 5' sequences complementary to the attached oligonucieotide, fail tc hybridize and therefore are not retained. The method makes it ossible to purify gram quantifies of synthetic eoxyribonuclcic acids or ribonucleic acids and sequences which have modifications, such as on
~
the phosphate backbone. The support-bound nucleotide sec~uences are stable under conditions of purification and therefore can be reused.
5352580 SELECTIVE DETECTION OF MYCOBACTERIA BY NUCLEICACID PROBES DERIVED FROM MYCOBACTERIUM KANSASII Patricia A Spears, Daryl Shank assigned to Becton Dickinson and Company Oligonucleotide probes derived from Mycobacteria K a n ~ i i capable of selectively hybridizing to Mycobucteria nucleic acid are disclosed. The oligonucleotide probe is selected from the group consisting of: (a) an oligonucieotide probe consisting essentially of the DNA sequence given herein as SEQ ID NO:I (MKI4); (b) oligonucleotide probes comprising fragments of MKI4 which retain the capabifity of MK14 of selectively hybridizing to Mycobacteria nucleic acid; (c) ol/gnnucleotide probes which hybridize to MKI4 and are capable of selectively hybridizing to Mycobacteria nucleic acid; and (d) oligonucleotide probes which are complementary to any of the foregoing and are capable of selectively hybridizing to Mycobacteria nucleic acid. The probes are useful in nucleic acid amplification and hybridization assays for genus-specific detection of the mycobacteria, Se~.fic detection of M. kansasii and specific on of the slow-growing mycobacteria. Methods of using the probes to detect and amplify Mycobacteria nucleic acid and kits containing the same are also disclosed.
5352581 SENSITIVE YEAST GENETIC SYSTEM FOR IDENTIFYING AGENTS CAUSING DOUBLESTRANDED DNA DAMAGE Michael A Resnick, Torston Nilsson-Tillgren assigned to United States/National Institutes of Health A sensitive, yeast-based genetic system for identifying agents causing double-strand DNA damage is described. The system comprises a yeast strain containing either chromosomes having divergent (homenlogous) DNA sequences, a single nonhomologous chromosome or a single artificial chromosome with suitable genetic markers so that double-strand damage leading to the loss of such chromosome
PATENT ABSTRACTS due to the inability to undergo recombinational repair with a homolog is detected.
5352587 COMPOSITIONS AND METHODS FOR THE SYNTHESIS OF NATRIURETIC PROTEIN RECEPTOR B AND METHODS OF USE
derivatives of bFGF can act as antagonists and/ or superagonists of the wild type molecule in the angiogenic process. These derivatives, as well as wild type bFGF, may be prepared by the use of strains or E. coil which have been transformed with plasmids carrying nucleotide sequence coding for human and bovine bFGF and their derivatives,
Ming-Sh Chang, David V Goeddel, David G Lowe, Newbury Park, Canada assigned to Genentech Inc Described are the amino acid sequence of natriuretic peptide receptor B (NPRB) and DNA encoding NPRB. Also disclosed are expression vectors and cells transformed to express the NPRB, DNA encoding NPRB and diagnostic and therapeutic uses for the NPRB and the DNA encoding NPRB.
5~25~ STREPTOCOCCAL IMMUNOGLOBULIN A BINDING PROTEIN ENCODED BY EMML2.2
265
5352592 MICROBIOLOGICAL PROCESS FOR THE PRODUCTION OF 5HYDROXYPYRAZINE CARBOXYLIC ACID Andreas Kiener, Visp, Switzerland assigned to Lonza Ltd A microbiological process for the production of 5-hydroxypyraziuec~boxylic acid and/or its salts with microorganisms of the strain Agrobacterium sp. DSM 6336 or descendants thereof or mutants thereof, utilizing 3-cyanopyridine.
Vincent A Fischetti, Debra E Bessen assigned to Rockefeller University The subject invention concerns a novel polynuclentide sequence cloned from emm2.2 gene of a Group A streptococcus, Type II strain which codes for an IgA-binding protein, ML2.2. A process for produdng the protein is given. The invention also concerns the protein in an immunoadsorbent and as a tracer for use in measuring and purifying IgA. Kits an: given comprising the immunoadsorbent and the tracer form of the protein.
5~2~9 DELETION MUTANT OF BASIC FIBROBLAST GROWTH FACTOR AND PRODUCTION THEREOF Laur Bergonzonl, Antonella Isuchi, Paolo Sarmientos, Gilles Cauet, Milan, Italy assigned to Farmitalia Carlo Erba S R L The present invention relates to the production, by recombinant DNA techniques, of derivatives of basic fibroblast growth factor (bFGF). These
5352595 MYOD
REGULATORY
REGION
Stephen J Tapscott, Harold M Weintranb, Theodore D Palmer assigned to Fred Hutchinson Cancer Research Center Isolated DNA or RNA molecules capable o f hybridizing under stringent conditions to the myoD regulatory region, its proximal promoter and distal enhancer regulatory regions, and regulatory elements within the proximal and distal regions for binding basic helix-loop-helix proteins, MyoD, and proteins binding at SPI, API, CAAT, M-CAT, CArG, and MEF sites in DNA. DNA or RNA expression vectors for introducing a geue into a cell under the regulatory control of the myoD regulatory region. Transduced or transfected pre-musele cells, myocytes, or myoblasts. Methods of inducing a muscle phenotype in a non-muscle cell, positively selecting for a cells expressing MyoD, and negatively selecting for cells expressing MyoD.