5358639 Method of analyzing hemoglobins

PATENT ABSTRACTS

5356790 HIGHLY SENSITIVE ASSAY METHOD FOR MYO-INOSITOL, COMPOSITION FOR PRACTICING SAME, NOVEL MYO-INOSITOL DEHYDROGENASE, AND

PROCESS FOR P R O D U C I N G SAME Shiger Ueda, Mamoru Takahashi, Hideo Misaki, Shigeyuk Imamura, Kazuo Matsuura, Shizuoka, Japan assigned to Asahi Kasei Kogyo Kabushiki Kaisha Myo-inositol in a specimen is assayed by reacting a specimen containing myo-inositol with: a) myo-inositol dehydrogenase using a thio-NADP group or thio-NAD group and an NADP group or NAD group as coenzymes, and which catalyzes a reversible reaction forming myo-inosose from myo-inositol, b ) AI and c) BI to effect a cycling reaction See Patent for Chemical Structure wherein AI is a thio-NADP group, thioNAD group, NADP group or NAD group, A2 is a reduced form of AI, when AI is a thio-NADP group or thioNAD group, BI is a reduced NADP group or reduced NAD group and when A1 is an NADP group or N A D group, BI is a reduced thioNADP group or reduced thioNAD group, and wherein B2 is an oxidized form of B1. The change in the amount of A2 generated or BI consumed by the cycling reaction is measured to perform the assay. A composition for performing the assay comprises the above myoinositol dehydrogenase, as well as the above components AI and B1. The myo-inositol dehydrogenase can be produced by culturing a suitable microorganism belonging to genus Bacillus, particularly Bacillus sp. No. 3 FERM BP-3013.

5356791 ASSAY FOR DETERMINING THE EFFICACY OF PAFACETHER AND/OR LA-PAF ANTAGONISTS

Ruth Korth, D 8000, Mfederal Republic Of Germana According to the invention, a procedure for determininf the efficacy of paf-acether and/or LA-paf antagonists using lipoproteins, lipoprotein-associated paf (LA-pal), cholesterol or a mixture of lipoproteins, LA-paf and cholesterol is disclosed.

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5356792 BIOCHEMICAL OXYGEN DEMAND ANALYZER AND METHODS OF ANALYSIS USING KLEBSIELLA MICROORGANISMS Shigeru Maeda, Akira Ohki, Takeshi Sato, Naho Kato, Hirofumi Akano, Yoshiya Kawamura, Hatagaki Keizo, Yasush Takahashi, Mikio Yamada, Hajime Okumura, Kagoshima, Japan assigned to Nakano Vinegar Co Ltd The present invention relates to a BOD analyzer comprising a microbe sensor containing an oxygen electrode and a microbe membrane. The microbe membrane is made by immobilizing microorganisms belonging to the genus Klebsiella in membrane. Specifically, the present invention relates to a BOD analyzer comprising flow cell equipped with a microbe sensor col~taining an oxygen electrode and a microbe membrane; and a liquid passage which is connected with the entrance o f the flow cell and which is equipped with an outlet. The microbe membrane is made by immobilizing microorganisms belonging to Klcbsiella oxytom 12092 strain in a porous hydrophilic membrane having an average pore size of 0.65-3 mum in diameter by using at least one gelating agent selected from the group consisting of alginic acid or salts thereof, agar, gellan gum, xanthane gum, gelatine, caraenan, locust bean gum, methylcellulose, and pullulan. The present invention also relates to improved methods of analyzing BOD by using the BOD analyzer.

5356793 METHOD FOR TESTING THE SENSITIVITY OF ANTICANCER

DRUG Masahiro Koczuka, Naohito Kondo, Sachiko Oda, Hisayuk Kobayashi, Masayuki Yasutomi, Nara, Japan assigned to Nitta Gelatin Inc The present invention relates to a method of testing the sensitivity of cancer drugs with cancer cells cultured in vitro. Cancer cells are cultured in a collagen gel substrate. A wide variety of human cancer cell types readily proliferate in the collagen gel substrate, however, fibroblust cells proliferate as well. The measurement of the growth of the cancer cells is hindered by the presence of the fibroblast cells. The present invention solves this problem by counting the number of colonies with an image processor which selectively extracts the image