PATENT ABSTRACTS
5358854 METHOD, TEST MEDIA AND CHROMOGENIC COMPOUNDS FOR IDENTIFYING AND DIFFERENTIATING GENERAL COLIFORMS AND ESCHERICHIA COLI BACTERIA Wilfred J Ferguson assigned to Research Organics Inc A chromogeulc beta-galactosidase substrate producing an insoluble precipitate of a first color when reacted upon by beta-galactosidase and a chromogenic beta-glucuronidase substrate producing an insoluble precipitate of a second, contrasting color when reacted upon by betagiucuronidase are combined in test medium for quantitatively identifying and differentiating general coliforms and E. coil. The betagaluctosidase substrate 5-bromo-4-chloro-3indolylbeta-D-galactopyranoside which produces an indigo blue precipitate when reacted upon by beta-galactosidase may be used with one of the novel compounds 6-chloroindolylbeta-D-giucuronide, 4,6-dichloroindolyl-betaD-glucuronide, 6,7-dichloroindolyl-beta-Dgiucuronide, and 4,6, 7-trichloroindolyl-betaD-glucuronide, which produce mauve or magenta precipitates when reacted upon by betagiucuronidase. The beta-giucuronidase substrate 5-bromo-4chloro-3-indolyl-beta-Dgiucuroulde, which produces an indigo blue precipitate when reacted upon by betagiucuronidase may be used with one of the novel compounds 6-chloroindolylbeta-Dgalactoside, 4,6-dichloroindolyl-betaDgaluctmide, 6,7-dichloroindolyl-beta-Dgalactoside, and 4,6, 7-trichloroindolyl-beta-Dgalactoside which produce mauve or magenta precipitates when reacted upon by betagalactosidase. 5358855 INOSINIC ACID DEHYDROGENASE ASSAY Olivera T Markovic, Nenad Maxkovic, Jay Roberts, Charles Pnglia, Svetomir N Markovic assigned to The Medical College of Pennsylvania A cyto(histo)chemical technique for determining inosine 5'-monophosphate dehydrogenase (IMPD) presence and concentration within one or more morphologically classified or classifiable cells taken from an animal or a human subject. Cell monolayers are prepared, fixed and stained according to a specified procedure in which inosine 5'-monophosphate
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(as enzyme substrate), nicotinamide adenine dinucleotide (NAD), phenazine methosulfate (PMS) and nitro blue tetrazolium (NBT) as a diazonium salt together produce a granular, dark blue deposit of formazan generally proportional to the IMPD activity in the cell.Cells are evaluated either visually or by image analysis, with a computeri2~ image analyzer or other imaging system, according to accepted biostatistical methods and standards.
5358871 CULTURE
VESSEL
Timothy A Stevens, Edward F Mussi, Douglas P Henderson, Tadensz Tyndorf assigned to Becton Dickinson and Company Apparatus for growing cells or tissue in vitro and for supporting, positioning and/or centering cell culture inserts that are used in such ~Pvirocedures. The apparatus comprises a body th a plurafity of wells wherein a cell culture insert may be supported and centered without interfering with the growing of tissue culture in vitro. 5359033 CLEAVED DIMERS OF MULLERIAN INHIBITING SUBSTANCE-LIKE POLYPEPTIDES Richard L Cate, R Blak Pepinsky assigned to Biogen Inc This invention relates to cleaved dimers of Mullerian inhibiting substance-like polypeptides. More particularly, this invention relates to such directs, methods of producing them and methods of using them in the treatment of cancer and tumors, especially those of the female genital tract. The dimers of this invention are also useful in compositions and methods for contraception. 5359037 ANTIBODIES TO TNF BINDING PROTEIN I David Wallach, Hartmut Engelmann, Dan Aderka, Rehovot, Israel assigned to Yeda Research and Development Co Ltd Antibodies to TNF Binding Protein I (TBP-I) are provided that can be used in diagnostic assays and as pharmaceutical agents for either