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5363697 Scanning probe microscope, molecular processing method using the scanning probe microscope and DNA base arrangement detecting method
PATENT ABSTRACTS
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plasmids, methods of producing the hG-CSF pol.vpeptide derivatives using the microorganisms, a monoclonal antibody binding to the hG-CSF polypeptide derivative, and chemically modified hG-CSF or derivatives thereof are disclosed.
5362864
T R A N S - A C T I V A T I N G FACTOR-1 Chua Nam-Hai, Scarsdale, NY, UNITED STATES - The Trustees of the Rockefeller University The active portion of a trans-activating factor, TAF-1, has been identified, isolated and characterized. This proteinaceous factor binds to motif-I-like sequences previously identified within numerous plant promoter elements. TAF-1 may be engineered into cell culture systems or transgenic plants to increase or modulate the expression of heterologous genes fused to promoter elements containing one or more copies of cis-acting sequences known to bind TAF-1.
5362865 E N H A N C E D EXPRESSION IN PLANTS USING NONTRANSLATED LEADER SEQUENCES Austin Glenn D - Monsanto Company This invention provides DNA molecules which comprise 5' non-translated leader sequences derived from genes coding for heat shock proteins that enhance gene expression in plants when present in a chimeric gene. Plant cells and plants containing same are also provided herewith. Further provided is a method for enhancing gene expression in plants.
5362866 OLIGONUCLEOTIDE P O L Y M E R I C S U P P O R T SYSTEM W I T H AN O X I D A T I O N C L E A V A B L E LINK Arnold Lyle, San Diego, CA, UNITED STATES Molecular Biosystems Inc
A versatile polymeric support system for the synthesis of oligonucleotides is provided featuring a universal primer which allows chain elongation, in either the 3' or 5' direction, with any currently available DNA or RNA synthesis method, by a process which utilizes oxidatively cleaved primers to facilitate chain elongation and release. The support system is capable of withstanding mildly basic and acidic reaction conditions, while still permitting a convenient and quantitative release, either before or after removal of protecting groups from reactive groups, of synthesized oligonucleotides from a single polymeric support. Removal of the protecting groups before cleavage of the oligomer from the support permits the use of the immobilized oligomer as an affinity hybridization support for both isolating and detecting complementary polynucleic acids.
5363697
SCANNING PROBE MICROSCOPE, MOLECULAR PROCESSING M E T H O D USING T H E SCANNING P R O B E M I C R O S C O P E AND DNA BASE ARRANGEMENT DETECTING METHOD Nakagawa Tohr - Matsushita Electric Industrial Co Ltd This invention relates to a scanning probe microscope which examines or processes directly the structure of substance surfaces at the molecular or atomic level, and a method for processing molecules using a scanning probe microscope and a method for detecting DNA base arrangement. A scanning probe microscope has a probe approaching or contacting the substance surface to detect a physical quantity between the substance surface and the probe, wherein the physical quantity is what is interacted or chemically reacted between the substance surface and the molecules or atom groups which act as a sensor and are fixed on the probe, scanning at an atomic level of precision. Therefore, surface structure within a microscopic region can be examined or processed at the molecular or atomic level. A method for detecting DNA base arrangement with any one of three or four kinds of probes fixed with any one of four different kinds of molecules interacting four kinds of bases consisting of DNA, by approaching single stranded DNA fixed on a substrate, measuring the force and scanning by an atomic force microscope at an atomic level of precision.
80
plasmids, methods of producing the hG-CSF pol.vpeptide derivatives using the microorganisms, a monoclonal antibody binding to the hG-CSF polypeptide derivative, and chemically modified hG-CSF or derivatives thereof are disclosed.
5362864
T R A N S - A C T I V A T I N G FACTOR-1 Chua Nam-Hai, Scarsdale, NY, UNITED STATES - The Trustees of the Rockefeller University The active portion of a trans-activating factor, TAF-1, has been identified, isolated and characterized. This proteinaceous factor binds to motif-I-like sequences previously identified within numerous plant promoter elements. TAF-1 may be engineered into cell culture systems or transgenic plants to increase or modulate the expression of heterologous genes fused to promoter elements containing one or more copies of cis-acting sequences known to bind TAF-1.
5362865 E N H A N C E D EXPRESSION IN PLANTS USING NONTRANSLATED LEADER SEQUENCES Austin Glenn D - Monsanto Company This invention provides DNA molecules which comprise 5' non-translated leader sequences derived from genes coding for heat shock proteins that enhance gene expression in plants when present in a chimeric gene. Plant cells and plants containing same are also provided herewith. Further provided is a method for enhancing gene expression in plants.
5362866 OLIGONUCLEOTIDE P O L Y M E R I C S U P P O R T SYSTEM W I T H AN O X I D A T I O N C L E A V A B L E LINK Arnold Lyle, San Diego, CA, UNITED STATES Molecular Biosystems Inc
A versatile polymeric support system for the synthesis of oligonucleotides is provided featuring a universal primer which allows chain elongation, in either the 3' or 5' direction, with any currently available DNA or RNA synthesis method, by a process which utilizes oxidatively cleaved primers to facilitate chain elongation and release. The support system is capable of withstanding mildly basic and acidic reaction conditions, while still permitting a convenient and quantitative release, either before or after removal of protecting groups from reactive groups, of synthesized oligonucleotides from a single polymeric support. Removal of the protecting groups before cleavage of the oligomer from the support permits the use of the immobilized oligomer as an affinity hybridization support for both isolating and detecting complementary polynucleic acids.
5363697
SCANNING PROBE MICROSCOPE, MOLECULAR PROCESSING M E T H O D USING T H E SCANNING P R O B E M I C R O S C O P E AND DNA BASE ARRANGEMENT DETECTING METHOD Nakagawa Tohr - Matsushita Electric Industrial Co Ltd This invention relates to a scanning probe microscope which examines or processes directly the structure of substance surfaces at the molecular or atomic level, and a method for processing molecules using a scanning probe microscope and a method for detecting DNA base arrangement. A scanning probe microscope has a probe approaching or contacting the substance surface to detect a physical quantity between the substance surface and the probe, wherein the physical quantity is what is interacted or chemically reacted between the substance surface and the molecules or atom groups which act as a sensor and are fixed on the probe, scanning at an atomic level of precision. Therefore, surface structure within a microscopic region can be examined or processed at the molecular or atomic level. A method for detecting DNA base arrangement with any one of three or four kinds of probes fixed with any one of four different kinds of molecules interacting four kinds of bases consisting of DNA, by approaching single stranded DNA fixed on a substrate, measuring the force and scanning by an atomic force microscope at an atomic level of precision.