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5380655 Methods and compositions for the production of Haemophilus influenzae type B major outer membrane protein antigens
PATENT ABSTRACTS
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of the instant invention can be used in place of standard RNA or DNA oligomers. For example, the target-specific polymers of the invention can be used in a variety o f diagnostic assays for detection of RNA or DNA having a given target sequence. Further, the polymers of the invention also have potential use as therapeutic agents.
5380645 GENERALIZED METHOD FOR ASSESSMENT OF COLORECTAL CARCINOMA Vogelstein Bert Baltimore, MD, UNITED STATES Assigned to The Johns Hopkins University A method is provided for assessing the generalized genetic change which occurs during tumorigenesis. The method relies on measurement of loss of a large number of alleles from the chromosomes of the tumor cells. The alleles are RFLP markers. The role of any of the particular alleles in tumorigenesis need not be known. The amount of ailelic loss allows a prognosis to be made regarding tumor metastasis, tumor recurrence, and mortality.
5380653 EXPRESSION VECTORS AND METHODS FOR INTRACELLULAR PROTEIN PRODUCTION IN BASCILLUS Palva llkka Helsinki, FINLAND Assigned to The Finnish National Public Health Institute The invention relates to recombinant DNA molecules and to methods for producing proteins by means of said molecules. Particularly, the present invention relates to recombinant DNA molecules which are capable of being synthesized in Bacillus strain bacteria comprising the regulation and deleted non-functional signal sequence of the a-amylase gene of B. amyloliquefaciens, or a substantial part thereof, to which sequence a structural gene of any desired homologous or heterologous protein or peptide may be joined. These recombinant DNA molecules can be used, for example, to achieve intracelhilar expression of any desired protein or peptide in Bacillus s~ain bacteria.
5380655 METHODS AND COMPOSITIONS FOR THE PRODUCTION OF HAEMOPHILUS INFLUENZAE TYPE B MAJOR OUTER MEMBRANE PROTEIN ANTIGENS Hansen Eric J Piano, TX, UNITED STATES Assigned to Board of Regents The University of Texas S),stem Disclosed are DNA and other biological compositions, including biological cultures, for preparing Haemophihis influenzae antigens by recombinant means. The disclosed recombinant antigens are suitable for use in the preparation of immunogenic compositions for use in vaccination against various Haemophilus infhienzae type B infections. Particular embodiments disclosed include DNA segments which encode the P2 major outer membrane protein antigen, also referred to as the 39/38 protein, biological cultures transformed by these DNA segments, and the preparation of recombinant P2 antigen through the use of these biological cultures.
5380657 METHOD FOR ISOLATION OF INSERTION ELEMENTS FROM CORYNEFORM BACTERIA Schaefer Andrea; Seep-Feldhaus Anna-Hildegard; Jaeger Wolfgang; Kalinowski Joeru; Wohlleben Wolfgang; Puehler Alfred Bielefeld, GERMANY Assigned to Degussa Aktiengesellsehaft A method locating insertion elements (IS elements) or transposons in coryneform bacteria, a positive selection system suitable for the above, the IS elements found in this manner and their use, is disclosed. The method involves: (1) The construction of a non-self-transferrable vector mobilizable from an E. coil mobilizer strain which vector is composed of (a) A DNA segment containing a replicon functional in E. coil, (b) A second DNA segment containing the DNA fragment coding for the mobilization function (Mob site containing the oriT), (c) A third DNA segment which recombines bomologously in Gram-positive bacteria and/or contains a replicon functional in coryneform bacteria, (d) A D N A segment from Bacillus subtiliscontaining the sacB
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of the instant invention can be used in place of standard RNA or DNA oligomers. For example, the target-specific polymers of the invention can be used in a variety o f diagnostic assays for detection of RNA or DNA having a given target sequence. Further, the polymers of the invention also have potential use as therapeutic agents.
5380645 GENERALIZED METHOD FOR ASSESSMENT OF COLORECTAL CARCINOMA Vogelstein Bert Baltimore, MD, UNITED STATES Assigned to The Johns Hopkins University A method is provided for assessing the generalized genetic change which occurs during tumorigenesis. The method relies on measurement of loss of a large number of alleles from the chromosomes of the tumor cells. The alleles are RFLP markers. The role of any of the particular alleles in tumorigenesis need not be known. The amount of ailelic loss allows a prognosis to be made regarding tumor metastasis, tumor recurrence, and mortality.
5380653 EXPRESSION VECTORS AND METHODS FOR INTRACELLULAR PROTEIN PRODUCTION IN BASCILLUS Palva llkka Helsinki, FINLAND Assigned to The Finnish National Public Health Institute The invention relates to recombinant DNA molecules and to methods for producing proteins by means of said molecules. Particularly, the present invention relates to recombinant DNA molecules which are capable of being synthesized in Bacillus strain bacteria comprising the regulation and deleted non-functional signal sequence of the a-amylase gene of B. amyloliquefaciens, or a substantial part thereof, to which sequence a structural gene of any desired homologous or heterologous protein or peptide may be joined. These recombinant DNA molecules can be used, for example, to achieve intracelhilar expression of any desired protein or peptide in Bacillus s~ain bacteria.
5380655 METHODS AND COMPOSITIONS FOR THE PRODUCTION OF HAEMOPHILUS INFLUENZAE TYPE B MAJOR OUTER MEMBRANE PROTEIN ANTIGENS Hansen Eric J Piano, TX, UNITED STATES Assigned to Board of Regents The University of Texas S),stem Disclosed are DNA and other biological compositions, including biological cultures, for preparing Haemophihis influenzae antigens by recombinant means. The disclosed recombinant antigens are suitable for use in the preparation of immunogenic compositions for use in vaccination against various Haemophilus infhienzae type B infections. Particular embodiments disclosed include DNA segments which encode the P2 major outer membrane protein antigen, also referred to as the 39/38 protein, biological cultures transformed by these DNA segments, and the preparation of recombinant P2 antigen through the use of these biological cultures.
5380657 METHOD FOR ISOLATION OF INSERTION ELEMENTS FROM CORYNEFORM BACTERIA Schaefer Andrea; Seep-Feldhaus Anna-Hildegard; Jaeger Wolfgang; Kalinowski Joeru; Wohlleben Wolfgang; Puehler Alfred Bielefeld, GERMANY Assigned to Degussa Aktiengesellsehaft A method locating insertion elements (IS elements) or transposons in coryneform bacteria, a positive selection system suitable for the above, the IS elements found in this manner and their use, is disclosed. The method involves: (1) The construction of a non-self-transferrable vector mobilizable from an E. coil mobilizer strain which vector is composed of (a) A DNA segment containing a replicon functional in E. coil, (b) A second DNA segment containing the DNA fragment coding for the mobilization function (Mob site containing the oriT), (c) A third DNA segment which recombines bomologously in Gram-positive bacteria and/or contains a replicon functional in coryneform bacteria, (d) A D N A segment from Bacillus subtiliscontaining the sacB