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5384245 Stable, single-liquid alpha-amylase reagent
PATENT ABSTRACTS
5384240 BASE DISSOCIATION ASSAY Hyman Jones M Durham, NC, UNITED STATES Assigned to Akzo Nobel N V This invention relates to a novel reagent and a method of using the reagent in an immunoassay to detect antigens, particularly antigens immunocomplexed with their corresponding or cross-reacting antibodies. In particular, this reagent and method increase the detection of human immunodeficiency virus HIV-1 p24 core antigen.
5384241 SPECIFIC BINDING ASSAY COMPOUND WITH INHIBITIVE SELF-QUENCHING CHARACTERISTICS
781
Amplification, replication or detection of a predetermined target nucleic acid can be carried out using a unique primer composition. This composition comprises and aqueous mixture of a first oligonucleotide primer which is substantially complementary to a first nucleic acid sequence of the target, but which is suspected of having one or more mismatches with the target at or near its 3' end. Also included in the composition is one or more additional primers which are complementary to a nucleic acid sequence of the target. This sequence is either: (i) inclusive of only a lxx'tion of the first nucleic acid sequence, (ii) immediately adjacent to the first nucleic acid sequence, or (iii) removed from the first sequence by one or more bases, but which additional primer is capable of forming a primer extension product complementary to the first sequence. These composition components can be supplied as part of a diagnostic test kit which can include other regents if desired.
Kline Stanley Brooklyn, NY, UNITED STATES Assigned to Enzo Diagnostics Inc Disclosed is an assay system including a compound comprising an analyte-specific moiety having substituted thereon a polymer comprising plurality of self-quenching emitter moieties and a plurality of isocharged functionality separating the emitter moieties. The present invention provides compounds that overcome the undesirable effects of self-quenching when multiple emitter moieties are used for labelling of assay reagents. Avoidance of this self-quenehing phenomenon by the compounds of the invention makes it possible to introduce a more concentrated degree of labelling on to analyte-specific molecules such as oligo nucleotide probes, antibodies and other specific binding proteins and analyte-specific polysaccharides. Therefor, it is possible to effect greater assay sensitivity because the number of labels per recognition molecole(analyte-specific moiety) can be increased beyond the point previously possible without the reduction in signal caused by self-quenching.
5384243 METHOD FOR SCREENING AN AGENT FOR ITS ABILITY TO PREVENT CELL TRANSFORMATION Gutkind J Silvio; Robbins Keith C Silver Spring, MD, UNITED STATES Assigned to The United States of America as represented by the Department of Health and Human Services The present invention relates, in general, to a method of screening agents. In particular, the present invention relates to a method of testing the cancer preventing activity of a drug and of testing an agent for its ability to prevent cell transformation.
53842~ DIAGNOSTIC KIT, PRIMER COMPOSITION AND THEIR USE FOR REPLICATION OR DETECTION OF NUCLEIC ACIDS Oakes Fred T Rochester, NY, UNITED STATES Assigned to Eastman Kodak Company
5384245 STABLE, SINGLE-LIQUID ALPHA-AMYLASE REAGENT Kwan Shiag F Ventura, CA, UNITED STATES Assigned to Modrovich Ivan E
782
PATENT ABSTRACTS
In a single liquid alpha-amylase reagent composition comprising an aqueous solution of at least one substrate which is hydrolyzed when mixed with a sample of body fluid containing alpha-amylase to yield a detectable label, alphaglucosidase from Bacillus stearothermophilus is used to cooperate with the alpha-amylase in the formation of the detectable label and the composition being stable against degradation for a least 6 months at 2 degrees to 10 degrees C.
5384248 PROCESS FOR MEASURING AN ANALYTE WITH AN OXIDASE AND AN OXIDIZABLE COLOR REAGENT AND SURFACTANTS Sakata Yoshitsugu; Hanada Toshiro; Matsuda Ryosuke; Matsuda Yoshiyuki Otsu, JAPAN Assigned to Wako Pure Chemical Industries Ltd A substrate or an enzymatic activity in a body fluid can be measured accurately without influences of interfering substances such as bilirubin by reacting an oxidase corresponding to an analyte with the analyte or an oxidase corresponding to a substance produced by enzymatic reaction with the substance in a measuring system containing one or more cationic and/or amphoteric surfactants, followed by optical measurement of hydrogen peroxide produced by the reaction.
5384254 IMMOBILIZATION OF BIOMOLECULES ON A FLUOROCARBON SURFACE MODIFIED WITH A POLYOFLUOROALKYL) SUGAR REAGENT Arentzen Rene; Jadhav Prabhakar K; Kobos Robert; Smart Bruce Wilmington, DE, UNITED STATES Assigned to E I Du Pont de Nemours and Company Poly(fluoroalkyl) sugar reagents are prepared containing a sugar such as a monosaccharide or a disaocharide to which are bonded a plurality of fluoroalkyl anchor groups capable of attaching to
a fluorocarbon surface,and either a reactive group capable of covalent coupling to a biomolecule such as an enzyme or a charged group to form an ion-exchanger or a non-ionic group to give a neutral fluorosurfactant. A spacer may be between the reactive group and the sugar. The poly(fluoroalkyl) sugar reagents are strongly adsorbed onto fluorocarbon surfaces to provide supports for such applications as separation and immobilization of biomolecules such as enzymes, carrying out heterogeneous diagnostic assays, and preparation of biosensors.
5384255 UBIQUITIN CARRIER ENZYME E2-F1, PURIFICATION, PRODUCTION, AND USE Ciechanover Aaron J; Blumenfeld Nava; Gonen Hedva Haifa, ISRAEL Assigned to Rappaport Family Institute for Research in the Medical Sciences A method for isolating and purifying novel species of E2 ubiqttitin-carrier protein, designated E2-F1, is disclosed. A method for preparing enzymatically active fragments of E2-F1 enzyme is also disclosed. The use of purified E2-F1 to produce antibodies is also disclosed. The use of such E2-Fl-specific antibodies to detect the presence of E2-F1 in a biological sample, and to inhibit protein degradation are also disclosed. Recombinant DNA molecules which code for E2FI, and recombinant hosts and vectors which contain E2-FI coding sequences are also disclosed. The use of such recombinant hosts and vectors to produce E2-F1 protein is also disclosed. The use of purified E2-F1 to identify and to isolate E3 enzyme is also disclosed. Methods for screening substances for the ability to inhibit E2F1 enzyme activity are also disclosed.
5385143 APPARATUS FOR MEASURING PREDETERMINED DATA OF LIVING TISSUE Aoyngi Takuo Tokyo, JAPAN Assigned to Nihon Kohden Corporation Disclosed is an apparatus for measuring a predetermined data of a living tissue, such as an oxygen saturation, a dye dilution curve and level
5384240 BASE DISSOCIATION ASSAY Hyman Jones M Durham, NC, UNITED STATES Assigned to Akzo Nobel N V This invention relates to a novel reagent and a method of using the reagent in an immunoassay to detect antigens, particularly antigens immunocomplexed with their corresponding or cross-reacting antibodies. In particular, this reagent and method increase the detection of human immunodeficiency virus HIV-1 p24 core antigen.
5384241 SPECIFIC BINDING ASSAY COMPOUND WITH INHIBITIVE SELF-QUENCHING CHARACTERISTICS
781
Amplification, replication or detection of a predetermined target nucleic acid can be carried out using a unique primer composition. This composition comprises and aqueous mixture of a first oligonucleotide primer which is substantially complementary to a first nucleic acid sequence of the target, but which is suspected of having one or more mismatches with the target at or near its 3' end. Also included in the composition is one or more additional primers which are complementary to a nucleic acid sequence of the target. This sequence is either: (i) inclusive of only a lxx'tion of the first nucleic acid sequence, (ii) immediately adjacent to the first nucleic acid sequence, or (iii) removed from the first sequence by one or more bases, but which additional primer is capable of forming a primer extension product complementary to the first sequence. These composition components can be supplied as part of a diagnostic test kit which can include other regents if desired.
Kline Stanley Brooklyn, NY, UNITED STATES Assigned to Enzo Diagnostics Inc Disclosed is an assay system including a compound comprising an analyte-specific moiety having substituted thereon a polymer comprising plurality of self-quenching emitter moieties and a plurality of isocharged functionality separating the emitter moieties. The present invention provides compounds that overcome the undesirable effects of self-quenching when multiple emitter moieties are used for labelling of assay reagents. Avoidance of this self-quenehing phenomenon by the compounds of the invention makes it possible to introduce a more concentrated degree of labelling on to analyte-specific molecules such as oligo nucleotide probes, antibodies and other specific binding proteins and analyte-specific polysaccharides. Therefor, it is possible to effect greater assay sensitivity because the number of labels per recognition molecole(analyte-specific moiety) can be increased beyond the point previously possible without the reduction in signal caused by self-quenching.
5384243 METHOD FOR SCREENING AN AGENT FOR ITS ABILITY TO PREVENT CELL TRANSFORMATION Gutkind J Silvio; Robbins Keith C Silver Spring, MD, UNITED STATES Assigned to The United States of America as represented by the Department of Health and Human Services The present invention relates, in general, to a method of screening agents. In particular, the present invention relates to a method of testing the cancer preventing activity of a drug and of testing an agent for its ability to prevent cell transformation.
53842~ DIAGNOSTIC KIT, PRIMER COMPOSITION AND THEIR USE FOR REPLICATION OR DETECTION OF NUCLEIC ACIDS Oakes Fred T Rochester, NY, UNITED STATES Assigned to Eastman Kodak Company
5384245 STABLE, SINGLE-LIQUID ALPHA-AMYLASE REAGENT Kwan Shiag F Ventura, CA, UNITED STATES Assigned to Modrovich Ivan E
782
PATENT ABSTRACTS
In a single liquid alpha-amylase reagent composition comprising an aqueous solution of at least one substrate which is hydrolyzed when mixed with a sample of body fluid containing alpha-amylase to yield a detectable label, alphaglucosidase from Bacillus stearothermophilus is used to cooperate with the alpha-amylase in the formation of the detectable label and the composition being stable against degradation for a least 6 months at 2 degrees to 10 degrees C.
5384248 PROCESS FOR MEASURING AN ANALYTE WITH AN OXIDASE AND AN OXIDIZABLE COLOR REAGENT AND SURFACTANTS Sakata Yoshitsugu; Hanada Toshiro; Matsuda Ryosuke; Matsuda Yoshiyuki Otsu, JAPAN Assigned to Wako Pure Chemical Industries Ltd A substrate or an enzymatic activity in a body fluid can be measured accurately without influences of interfering substances such as bilirubin by reacting an oxidase corresponding to an analyte with the analyte or an oxidase corresponding to a substance produced by enzymatic reaction with the substance in a measuring system containing one or more cationic and/or amphoteric surfactants, followed by optical measurement of hydrogen peroxide produced by the reaction.
5384254 IMMOBILIZATION OF BIOMOLECULES ON A FLUOROCARBON SURFACE MODIFIED WITH A POLYOFLUOROALKYL) SUGAR REAGENT Arentzen Rene; Jadhav Prabhakar K; Kobos Robert; Smart Bruce Wilmington, DE, UNITED STATES Assigned to E I Du Pont de Nemours and Company Poly(fluoroalkyl) sugar reagents are prepared containing a sugar such as a monosaccharide or a disaocharide to which are bonded a plurality of fluoroalkyl anchor groups capable of attaching to
a fluorocarbon surface,and either a reactive group capable of covalent coupling to a biomolecule such as an enzyme or a charged group to form an ion-exchanger or a non-ionic group to give a neutral fluorosurfactant. A spacer may be between the reactive group and the sugar. The poly(fluoroalkyl) sugar reagents are strongly adsorbed onto fluorocarbon surfaces to provide supports for such applications as separation and immobilization of biomolecules such as enzymes, carrying out heterogeneous diagnostic assays, and preparation of biosensors.
5384255 UBIQUITIN CARRIER ENZYME E2-F1, PURIFICATION, PRODUCTION, AND USE Ciechanover Aaron J; Blumenfeld Nava; Gonen Hedva Haifa, ISRAEL Assigned to Rappaport Family Institute for Research in the Medical Sciences A method for isolating and purifying novel species of E2 ubiqttitin-carrier protein, designated E2-F1, is disclosed. A method for preparing enzymatically active fragments of E2-F1 enzyme is also disclosed. The use of purified E2-F1 to produce antibodies is also disclosed. The use of such E2-Fl-specific antibodies to detect the presence of E2-F1 in a biological sample, and to inhibit protein degradation are also disclosed. Recombinant DNA molecules which code for E2FI, and recombinant hosts and vectors which contain E2-FI coding sequences are also disclosed. The use of such recombinant hosts and vectors to produce E2-F1 protein is also disclosed. The use of purified E2-F1 to identify and to isolate E3 enzyme is also disclosed. Methods for screening substances for the ability to inhibit E2F1 enzyme activity are also disclosed.
5385143 APPARATUS FOR MEASURING PREDETERMINED DATA OF LIVING TISSUE Aoyngi Takuo Tokyo, JAPAN Assigned to Nihon Kohden Corporation Disclosed is an apparatus for measuring a predetermined data of a living tissue, such as an oxygen saturation, a dye dilution curve and level