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5389513 Method for detecting Listeria monocytogenes
558
PATENT ABSTRACTS
A transgenic plant is prepared by a method in which a plant cell is transformed with a chimaeric gene comprising a promoter and a gene encoding a polypeptide which displays the activity of an enzyme which regulates the amount of a metabolic intermediate in glycolysis or in a pathway for the synthesis or degradation of starch, sucrose or reducing sugar from a glycotyltic intermediate. In stored potatoes the subject of the invention an increased level of phosphofructokinase results in reduced accumulations of sugars in the tubers.
5387757 TOMATOES WITH REDUCED EXPRESSION OF POLYGALACTURONASE Bridges Inn G; Schuch Wolfgang W; Grierson Donald Siater, IA, UNITED STATES Assigned to Zeneca Limited Fruit, especially tomatoes, having lowered expression of fruit-softening enzymes caused by antisense gene expression, and seeds therefrom.
5389339 INTEGRAL BIOMOLECULE PREPARATION DEVICE
This invention provides a vaccine for the immunization of a vertebrate or invertebrate comprising an avirulent derivative of a pathogenic microbe said derivative being substantially incapable of producing functional adenylate cyclase and cyclic AMP receptor protein. The invention also provides a vaccine for the immunization of a vertebrate and invertebrate comprising a virulent derivative of a pathogenic microbe said derivative being substantially incapable of producing functional adenylate cyclase and cyclic AMP receptor protein while being capable of expressing a recombinant gene derived from a pathogen of said vertebrate to produce and antigen capable of inducing an immune response in said vertebrate against said pathogen.
5389512 M E T H O D FOR DETERMINING THE RELATIVE AMOUNT OF A VIRAL NUCLEIC ACID SEGMENT IN A SAMPLE BY THE POLYMERASE CHAIN REACTION Kwok Shirley Y; Sninsky John J San Ramon, CA, UNITED STATES Assigned to Hoffman-La Roche Inc
An integral biomolecule preparation device which accomplishes all of the nucleic acid separation steps, including centrifugation, reagent addition, and pipeting, automatically without human intervention to fully automate the DNA separation procedure.
The present invention provides a method for determining the relative amount of a nucleic acid segment in a sample by the polymerase chain reaction. The method involves the simultaneous amplification of the nucleic acid segment and a second nucleic acid segment present in the sample. The amount of amplified DNA from each segment is determined and compared to standard curves to determine the amount of the nucleic acid segment present in the sample before amplification expressed as a ratio of first segment to second segment. The method is especially preferred for determining the viral load, or copies of virus genome/host cell, in a sample of cells from an individual infected with a virus.
5389368
5389513
AVIRULENT MICROBES AND USES THEREOF
METHOD FOR DETECTING LISTERIA MONOCYTOGENES
Petschek Harry E; Kaufmann Henry; Quinlan Robert A; DeBonville David A; Sklar Martin; Danehy Ronald D; Foley Michael; Dobbs John McG; Banks David Lexington, MA, UNITED STATES Assigned to Enprotech Corporation
Gurtiss Roy St Louis, MO, UNITED STATES Assigned to Washington University
Baquero Fernando; Cossart Pascale SPAIN Assigned to Institut Pasteur
Madrid,
PATENT ABSTRACTS
A DNA probe is disclosed which is capable of hybridizing to a portion of the genome of pathogenic Listeria monocytogenes but which does not hybridize to portions of the genomes of other Listeria species and other humilytic bacteria. This probe is useful to identify food sources infected with Listeria monocytogenes and to distinguish these food services from those infected nearly with non-pathogenic Listeria species. In addition, methods for the detection of pathogenic Listeria in samples using the disclosed probes are provided.
5389514 M E T H O D FOR SPECIFICALLY ALTERING THE NUCLEOTIDE SEQUENCE OF RNA Taylor John Cheltenham, PA, UNITED STATES Assigned to Fox Chase Cancer Center A method is provided for specifically altering the nucleotide sequence of an RNA molecule. As performed in vitro the method comprises: (1) selecting a target sequence in an RNA molecule; (3) hybridizing the target sequence with a complementary nucleic acid, either DNA or RNA, thereby forming a double-stranded structure; (4) incubating the hybrid molecule with a cellular extract which contains components capable of specifically converting U to C in an RNA molecule, when the U is disposed within a doublestranded structure. Incubation of the hybrid molecule with cellular extracts comprising the RNA editing component results in a specific alteration of the nucleotide sequence of the RNA at the target sequence. The method of the invention may also be applied in vivo. Complementary nucleic acids are introduced into cells and hybridized to the target sequence to form a double-stranded structure. The RNA editing components already present in the cell may then specifically convert U to C in the target sequence resulting in an alteration in the target RNA molecule.
5389515 ISOLATED NUCLEOTIDE SEQUENCES FOR IDENTIFYING NEISSERIA GONORRHOEAE Chmelo Rich; Foltz Lisa Indianapolis, IN, UNITED STATES Assigned to Boehringer Mannheim Corporation
559
Isolated nucleic acid molecules capable of hybridizing to rRNA sequences of Neisseria Gonorrhoeae and not to rRNA sequences of nonNeisseria Gonorrhoeae along with methods utilizing such probes for the detection of Neisseria Gonorrhoeae in clinical and other biological samples.
5389517 SPECIFIC ANTIBODIES AGAINST THE DNA-BINDING DOMAIN OF AND IMMUNOASSAYS TO DETERMINE THE PRESENCE AND FUNCTIONAL STATUS OF ESTROGEN RECEPTOR PROTEINS Wotiz Herbert H; Traish Abdulmaged M Milton, MA, UNITED STATES Assigned to Trustees of Boston University The present invention provides unique prepared immunogens, site-specific polyclonal antisera and monoclonal antibodies against the DNA-binding domain of estrogen receptor protein, and immunoassay to determine the functional status of estrogen receptors in a cellular sample. Collectively or individually the component parts of the invention provide the ability not only to identify accurately the presence of human estrogen receptor but also the capability of determining whether the estrogen receptor exists in a functional or non-functional state.
5389525 DNA-MOLECULES CODING FOR FMDH CONTROL REGIONS AND STRUCTURAL GENE FOR A PROTEIN HAVING FMDHACTIVITY AND THEIR USE THEREOF Hollenberg Cornelius P; Janowicz Zbigniew Dusseldorf, GERMANY Assigned to Rhein Biotech The invention relates to DNA-molecules comprising DNA-sequences encoding control regions and the structural gene for a protein having formate dehydrogenase (FMDH) activity. Said DNA-molecules may be combined with DNA-sequences encoding foreign genes so as to bring these genes under the stringent control of the
PATENT ABSTRACTS
A transgenic plant is prepared by a method in which a plant cell is transformed with a chimaeric gene comprising a promoter and a gene encoding a polypeptide which displays the activity of an enzyme which regulates the amount of a metabolic intermediate in glycolysis or in a pathway for the synthesis or degradation of starch, sucrose or reducing sugar from a glycotyltic intermediate. In stored potatoes the subject of the invention an increased level of phosphofructokinase results in reduced accumulations of sugars in the tubers.
5387757 TOMATOES WITH REDUCED EXPRESSION OF POLYGALACTURONASE Bridges Inn G; Schuch Wolfgang W; Grierson Donald Siater, IA, UNITED STATES Assigned to Zeneca Limited Fruit, especially tomatoes, having lowered expression of fruit-softening enzymes caused by antisense gene expression, and seeds therefrom.
5389339 INTEGRAL BIOMOLECULE PREPARATION DEVICE
This invention provides a vaccine for the immunization of a vertebrate or invertebrate comprising an avirulent derivative of a pathogenic microbe said derivative being substantially incapable of producing functional adenylate cyclase and cyclic AMP receptor protein. The invention also provides a vaccine for the immunization of a vertebrate and invertebrate comprising a virulent derivative of a pathogenic microbe said derivative being substantially incapable of producing functional adenylate cyclase and cyclic AMP receptor protein while being capable of expressing a recombinant gene derived from a pathogen of said vertebrate to produce and antigen capable of inducing an immune response in said vertebrate against said pathogen.
5389512 M E T H O D FOR DETERMINING THE RELATIVE AMOUNT OF A VIRAL NUCLEIC ACID SEGMENT IN A SAMPLE BY THE POLYMERASE CHAIN REACTION Kwok Shirley Y; Sninsky John J San Ramon, CA, UNITED STATES Assigned to Hoffman-La Roche Inc
An integral biomolecule preparation device which accomplishes all of the nucleic acid separation steps, including centrifugation, reagent addition, and pipeting, automatically without human intervention to fully automate the DNA separation procedure.
The present invention provides a method for determining the relative amount of a nucleic acid segment in a sample by the polymerase chain reaction. The method involves the simultaneous amplification of the nucleic acid segment and a second nucleic acid segment present in the sample. The amount of amplified DNA from each segment is determined and compared to standard curves to determine the amount of the nucleic acid segment present in the sample before amplification expressed as a ratio of first segment to second segment. The method is especially preferred for determining the viral load, or copies of virus genome/host cell, in a sample of cells from an individual infected with a virus.
5389368
5389513
AVIRULENT MICROBES AND USES THEREOF
METHOD FOR DETECTING LISTERIA MONOCYTOGENES
Petschek Harry E; Kaufmann Henry; Quinlan Robert A; DeBonville David A; Sklar Martin; Danehy Ronald D; Foley Michael; Dobbs John McG; Banks David Lexington, MA, UNITED STATES Assigned to Enprotech Corporation
Gurtiss Roy St Louis, MO, UNITED STATES Assigned to Washington University
Baquero Fernando; Cossart Pascale SPAIN Assigned to Institut Pasteur
Madrid,
PATENT ABSTRACTS
A DNA probe is disclosed which is capable of hybridizing to a portion of the genome of pathogenic Listeria monocytogenes but which does not hybridize to portions of the genomes of other Listeria species and other humilytic bacteria. This probe is useful to identify food sources infected with Listeria monocytogenes and to distinguish these food services from those infected nearly with non-pathogenic Listeria species. In addition, methods for the detection of pathogenic Listeria in samples using the disclosed probes are provided.
5389514 M E T H O D FOR SPECIFICALLY ALTERING THE NUCLEOTIDE SEQUENCE OF RNA Taylor John Cheltenham, PA, UNITED STATES Assigned to Fox Chase Cancer Center A method is provided for specifically altering the nucleotide sequence of an RNA molecule. As performed in vitro the method comprises: (1) selecting a target sequence in an RNA molecule; (3) hybridizing the target sequence with a complementary nucleic acid, either DNA or RNA, thereby forming a double-stranded structure; (4) incubating the hybrid molecule with a cellular extract which contains components capable of specifically converting U to C in an RNA molecule, when the U is disposed within a doublestranded structure. Incubation of the hybrid molecule with cellular extracts comprising the RNA editing component results in a specific alteration of the nucleotide sequence of the RNA at the target sequence. The method of the invention may also be applied in vivo. Complementary nucleic acids are introduced into cells and hybridized to the target sequence to form a double-stranded structure. The RNA editing components already present in the cell may then specifically convert U to C in the target sequence resulting in an alteration in the target RNA molecule.
5389515 ISOLATED NUCLEOTIDE SEQUENCES FOR IDENTIFYING NEISSERIA GONORRHOEAE Chmelo Rich; Foltz Lisa Indianapolis, IN, UNITED STATES Assigned to Boehringer Mannheim Corporation
559
Isolated nucleic acid molecules capable of hybridizing to rRNA sequences of Neisseria Gonorrhoeae and not to rRNA sequences of nonNeisseria Gonorrhoeae along with methods utilizing such probes for the detection of Neisseria Gonorrhoeae in clinical and other biological samples.
5389517 SPECIFIC ANTIBODIES AGAINST THE DNA-BINDING DOMAIN OF AND IMMUNOASSAYS TO DETERMINE THE PRESENCE AND FUNCTIONAL STATUS OF ESTROGEN RECEPTOR PROTEINS Wotiz Herbert H; Traish Abdulmaged M Milton, MA, UNITED STATES Assigned to Trustees of Boston University The present invention provides unique prepared immunogens, site-specific polyclonal antisera and monoclonal antibodies against the DNA-binding domain of estrogen receptor protein, and immunoassay to determine the functional status of estrogen receptors in a cellular sample. Collectively or individually the component parts of the invention provide the ability not only to identify accurately the presence of human estrogen receptor but also the capability of determining whether the estrogen receptor exists in a functional or non-functional state.
5389525 DNA-MOLECULES CODING FOR FMDH CONTROL REGIONS AND STRUCTURAL GENE FOR A PROTEIN HAVING FMDHACTIVITY AND THEIR USE THEREOF Hollenberg Cornelius P; Janowicz Zbigniew Dusseldorf, GERMANY Assigned to Rhein Biotech The invention relates to DNA-molecules comprising DNA-sequences encoding control regions and the structural gene for a protein having formate dehydrogenase (FMDH) activity. Said DNA-molecules may be combined with DNA-sequences encoding foreign genes so as to bring these genes under the stringent control of the