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5389547 Pretreating reagent, pretreatment method, assay with pretreated samples, assay kit and method of diagnosing infectious diseases
PATENT ABSTRACTS
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encapsulated within a semipermeable, polymeric membrane by co-extruding an aqueous cell suspension and a polymeric solution through a common port to form a tubular extrudate having a polymeric outer coating which encapsulates the cell suspension. For example, the cell suspension and the polymeric solution can be extruded through a common extrusion port having at least two concentric bores, such that the cell suspension is extruded through the inner bore and the polymeric solution is extruded through the outer bore. The polymeric solution coagulates to form an outer coating. As the outer coating is formed, the ends of the tubular extrudate can be sealed to form a cell capsule. In one embodiment, the tubular extrudate is sealed at intervals to define separate cell compartments connected by polymeric links.
5389544 METHOD FOR COUNTING LIVING CELLS OF MICROBES AND APPARATUS THEREOF Sugata Kiyoshi; Ueda Ryohei; Doi Takashi; Onishi Takashi; Matsumoto Kazunori Yokohama, JAPAN Assigned to Mitsubishi Jukogyo Kabushild Kaisha A method for counting living cells of microbes in a fluid sample continuously while flowing the sample using an apparatus which comprises a system for supplying at a predetermined rate to the flow line of the sample a reagent, such as, a derivative of fluoreseein, capable of reacting with one or more substances intrinsic of the living cell, such as enzyme, to form an accumulative fluorescent product within the living cells; a reactor inserted in the flow line of the sample and being provided for the reaction of the reagent with the cell-intrinsic substance in the living cells; a photometric detection system arranged subsequent to the reactor for detecting fluorescence emitted as individual luminous point from the fluorescent product in each of the living cells floating in the flowing sample upon irradiation of the fluorescent product by an exciting ray; and an electronic unit including a pulse counter for counting electric pulses produced from each fluorescence from the luminous point.
5389547 PRETREATING REAGENT, PRETREATMENT METHOD, ASSAY WITH PRETREATED SAMPLES, ASSAY KIT AND METHOD OF DIAGNOSING INFECTIOUS DISEASES Tanaka Shigenori; Tamura H_iroshi Tokyo, JAPAN Assigned to Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation) A reagent denatures or eliminates factors interfering with biochemical reactions by simple treatment without requiring separation of any denatured product precipitate. The reagent makes it possible to assay, in particular, beta-glucan and endotoxin in blood-derived samples rapidly and efficiently with high sensitivity. The reagent includes a hexadimethrine compound and an alkali metal hydroxide or an alkali metal hydroxide as a main component. A method for assaying a substance specifically reacting with a Limulns reagent utilizing the reagent, an assay kit including at least the reagent and a Limulus reagent, and a method of diagnosing infectious diseases based on the results obtained by the assay method are also provided.
5389609 ANTIFUNGAL PREPARATIONS, AND PROCESS FOR MAKING SUCH PREPARATIONS Woloshuk Charles; Melchers Leo; Cornelissen Bernardus J; Meuleulaoff Elisabeth J; SelaBuurlage Marianne; Van Den Elzen Petrus J Raleigh, NC, UNITED STATES Assigned to Mogen International N V PCT No. PCT/NL91/00089 Sec. 371 Date Apr. 6, 1992 See. 102(e) Date Apr. 6, 1992 PCT Filed May 31, 1991 PCT Pub. No. WO91/18984 PCT Pub. Date Dec. 12, 1991. New methods are provided for the identification and purification of proteins from plants with an inhibitory effect on pathogens and/or pests, such as insects, nematodes, fungi, and bacteria, using a combination of non-denaturing protein fracdonadon techniques on leaf-extracts of #ants which were treated to develop induced resistance and an antipathogenic assay. Also provided are antifungal proteins obtained by the method, recombinant nucleotide sequences encoding the
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encapsulated within a semipermeable, polymeric membrane by co-extruding an aqueous cell suspension and a polymeric solution through a common port to form a tubular extrudate having a polymeric outer coating which encapsulates the cell suspension. For example, the cell suspension and the polymeric solution can be extruded through a common extrusion port having at least two concentric bores, such that the cell suspension is extruded through the inner bore and the polymeric solution is extruded through the outer bore. The polymeric solution coagulates to form an outer coating. As the outer coating is formed, the ends of the tubular extrudate can be sealed to form a cell capsule. In one embodiment, the tubular extrudate is sealed at intervals to define separate cell compartments connected by polymeric links.
5389544 METHOD FOR COUNTING LIVING CELLS OF MICROBES AND APPARATUS THEREOF Sugata Kiyoshi; Ueda Ryohei; Doi Takashi; Onishi Takashi; Matsumoto Kazunori Yokohama, JAPAN Assigned to Mitsubishi Jukogyo Kabushild Kaisha A method for counting living cells of microbes in a fluid sample continuously while flowing the sample using an apparatus which comprises a system for supplying at a predetermined rate to the flow line of the sample a reagent, such as, a derivative of fluoreseein, capable of reacting with one or more substances intrinsic of the living cell, such as enzyme, to form an accumulative fluorescent product within the living cells; a reactor inserted in the flow line of the sample and being provided for the reaction of the reagent with the cell-intrinsic substance in the living cells; a photometric detection system arranged subsequent to the reactor for detecting fluorescence emitted as individual luminous point from the fluorescent product in each of the living cells floating in the flowing sample upon irradiation of the fluorescent product by an exciting ray; and an electronic unit including a pulse counter for counting electric pulses produced from each fluorescence from the luminous point.
5389547 PRETREATING REAGENT, PRETREATMENT METHOD, ASSAY WITH PRETREATED SAMPLES, ASSAY KIT AND METHOD OF DIAGNOSING INFECTIOUS DISEASES Tanaka Shigenori; Tamura H_iroshi Tokyo, JAPAN Assigned to Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation) A reagent denatures or eliminates factors interfering with biochemical reactions by simple treatment without requiring separation of any denatured product precipitate. The reagent makes it possible to assay, in particular, beta-glucan and endotoxin in blood-derived samples rapidly and efficiently with high sensitivity. The reagent includes a hexadimethrine compound and an alkali metal hydroxide or an alkali metal hydroxide as a main component. A method for assaying a substance specifically reacting with a Limulns reagent utilizing the reagent, an assay kit including at least the reagent and a Limulus reagent, and a method of diagnosing infectious diseases based on the results obtained by the assay method are also provided.
5389609 ANTIFUNGAL PREPARATIONS, AND PROCESS FOR MAKING SUCH PREPARATIONS Woloshuk Charles; Melchers Leo; Cornelissen Bernardus J; Meuleulaoff Elisabeth J; SelaBuurlage Marianne; Van Den Elzen Petrus J Raleigh, NC, UNITED STATES Assigned to Mogen International N V PCT No. PCT/NL91/00089 Sec. 371 Date Apr. 6, 1992 See. 102(e) Date Apr. 6, 1992 PCT Filed May 31, 1991 PCT Pub. No. WO91/18984 PCT Pub. Date Dec. 12, 1991. New methods are provided for the identification and purification of proteins from plants with an inhibitory effect on pathogens and/or pests, such as insects, nematodes, fungi, and bacteria, using a combination of non-denaturing protein fracdonadon techniques on leaf-extracts of #ants which were treated to develop induced resistance and an antipathogenic assay. Also provided are antifungal proteins obtained by the method, recombinant nucleotide sequences encoding the