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539 DISORDERS OF PURINE METABOLISM ENZYMES IN ATHEROSCLEROSIS
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Atherosclerosis Supplements 12, no. 1 (2011) 13–184
after further dividing CAD group using tHcy below 15 mM as reference, Trx activity was significantly decreased in CAD group, and was inversely associated with tHcy levels (r = −0.199, P < 0.05) that was, however, weakly positively associated with TrxR activity. Neither lipids nor glucose significantly affected Trx/TrxR activity. Association of CAD severity with low Trx plus high tHcy was strong (r = −0.458, P < 0.001), but with high tHcy alone was rather weak (r = 0.125, P = 0.225). Conclusion: In CAD patients, homocysteine-induced Trx falling appears to be involved in the disease severity. 536 THE EFFECT OF ROSUVASTATIN TREATMENT ON D-DIMER LEVELS IN OBESE PATIENTS WITH PRIMARY DYSLIPIDEMIA D.I. Agapakis1 , C. Savopoulos2 , K. Tziomalos2 , E.V. Massa2 , N. Katsiki2 , E. Satsoglou1 , A.I. Hatzitolios2 . 1 Internal Medicine, General Hospital of Goumenissa, Goumenissa, 2 First Propedeutic Department of Internal Medicine, Aristotle University of Thessaloniki, Hospital AHEPA, Thessalon´ıki, Greece Introduction: Risk of thromboembolic complications is increased in obese subjects. Current literature suggests that statin therapy decreases the risk of venous thrombosis and reduces plasma D-dimer (D-d) levels, a marker of hypercoagulable state. Aim: To evaluate the effect of rosuvastatin on D-d levels in patients with uncontrolled primary dyslipidemia and elevated body mass index (BMI). Material and Methods: 37 patients with uncontrolled primary dyslipidemia under hypolipidemic diet treatment, included in the study. The control group was 23 normolipidemic healthy subjects with similar characteristics (age, sex, BMI) with the study group. Dyslipidemic patients were treated with 10 mg/day rosuvastatin for 9 weeks. Lipids parameters and D-d levels (Triage D-Dimer test kit) were measured at baseline for both groups and after rosuvastatin treatment in dyslipidemic group. Results: At baseline, both groups had no significant differences in D-d levels (493±175 ng/ml vs 457±237 ng/ml, p > 0.05). In addition to lipid parameters, the D-d levels significantly decreased after rosuvastatin treatment from 493±175 ng/ml to 367±137 ng/ml, p < 0.001 Conclusions: These data support that beyond the hypolipidemic effect rosuvastatin also possesses significant anti-coagulation properties and could play a role in reducing thromboembolic complications in primary hyperlipidemic obese patients. 537 THE LINK BETWEEN CARDIAC AUTONOMIC FUNCTION AND MARKERS OF OXIDATIVE STRESS IN CORONAY ARTERY DISEASE (CAD) S. Radovanovic, L. Stojanovic, M. Zdravkovic, M. Krotin, B. Milovanovic. University Hospital Bezanijska Kosa, Belgrade, Serbia Background: The objective was to examine cardiovascular autonomic (cANS) function and its potential relationships with markers of oxidative stress in coronary artery disease (CAD) patients Methods: A total of 120 CAD patients mean age, 59±7 years (74 men and 46 women) were included in the stady. All of them had cANS function assessed via heart rate variability (HRV) methods during resting conditions. Standard time-domain and frequency-domain measures [high-frequency normalized units (HFnu; measure of parasympathetic nervous system activity) and low frequency:high-frequency ratio (LF:HF; overall sympathovagal balance)] of HRV were calculated. Plasma malondialdehyde (MDA), protein thiol (P-SH) and reactive carbonyl groups (RCD), together with glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities were determined spectrophotometrically and/or by ELISA methods and correlated with timedomein parameters of HRV (mean RR interval, SDNN, SDANN, rMSSD and pNN50%) as well as with frequency-domain measures (ULF, VLF, LF, LF/HF) Results: Significantly corelation was found between SDNN and GSH-Px (r = 0.221, p = 0.039) as well as between pNN50LF and MDA (r = 0.215, p = 0.045). By analyzing the corelation of frequency-domain measures with oxidative demage of proteins (P-SH) were observed following correlation: P-SH and ULF (r = −0.234, p = 0.034); P-SH and VLF (r = −0.219, p = 0.046); P-SH and LF/HF (r = −0.247, p = 0.025). LF was significantly related to GPx (r = 0.212, p = 0.054). Conclusion: We can conclude that cardiovascular autonomic (cANS) dysfunction assessed via heart rate variability was significantly related to increased oxidative stress in chronic heart failure.
Poster presentations
538 ISCHEMIA MODIFIED ALBUMIN (IMA) IN POSTMENOPAUSAL WOMEN WITH OR WITHOUT CORONARY ARTERY DISEASE (CAD) IN RELATION TO BMI AND OGTT K. Kazanis1 , M. Dalamaga1 , E. Kassi1 , G. Merantzi2 , I. Vagionas2 , G. Jullien2 , A. Dionyssiou-Asteriou1 . 1 Clinical Biochemistry, University of Athens, Medical School, 2 Laboratory of Biochemistry, Evangelismos General Hospital, Athens, Greece Background: IMA is a new biomarker of oxidative processes. Its measurement by the cobalt albumin binding assay is reported as an assessment of atherosclerotic burden. In postmenopausal women with or without CAD, we assessed IMA and determined its correlation with BMI, hsCRP and hyperglycaemia. Methods: 120 postmenopausal women, aged 43−80 years, were divided into three groups: Group A (n = 30) with BMI > 26−32 (15 with normal OGTT and 15 with impaired OGTT), Group B (n = 60) with BMI > 21−25 and Group C (n = 30) with BMI > 23−29 and documented CAD. Glucose, insulin, IMA, albumin and hsCRP were measured in an automated analyzer (Roche). The ratio IMA/albumin, HOMA and QUICKI were calculated. Results: IMA and IMA/albumin were significantly elevated in Group A compared to Group B (p < 0.001) but similar to those of Group C (p=1, p = 0.33 respectively). In Group A, there wasn’t any difference between subjects with normal and impaired OGTT. In Group A and B, serum hsCRP was similar but lower in comparison to Group C (p < 0.001). Glucose, insulin and HOMA were elevated in Group A compared to Group B while QUICKI was lower (p < 0.001). In Group A, IMA was positively correlated with BMI (p = 0.03), hsCRP (p < 0.001), insulin and HOMA and negatively with QUICKI (p < 0.01). Regression analysis revealed that obesity was the strongest predictor of IMA. Conclusions: Serum IMA in overweight women without CAD was similar to those with CAD. Whether IMA measurement provides an early assessment of atherosclerotic burden before clinical evidence of CAD needs further investigation. 539 DISORDERS OF PURINE METABOLISM ENZYMES IN ATHEROSCLEROSIS S. Kundalic1 , B. Kundalic2 , T. Ristic1 , D. Stankovic-Ferlez1 . 1 Centre of Medical Biochemistry, Clinical Centre Nis, 2 Faculty of Medicine, University of Nis, Nis, Serbia Since purine metabolism is important for endothelial cells’ normal function, purine catabolism enzymes have an especial place in damaged endothelium of atherosclerotic blood vessels in hyperlipidemias. The activities of 5’-nucleotidase (5’-NT), adenosine deaminase (ADA) and xanthine oxidase (XO) were investigated in atherosclerotic patients. This study obtained 55 patients with atherosclerosis whose results were compared to 46 healthy individuals. According to the values of lipid parameters: cholesterol, triglycerides, HDL-cholesterol (HDL-c) and LDL-cholesterol, the patients were allocated into hyperlipoproteinemic types by Fredrickson, while type subgroups were formed based on the values of hypercholesterolemia and hypertriglyceridemia. The most frequent types of hyperlipoproteinemias were IIa, IIb and type IV. Activity of 5’-NT was significantly increased in serum of patients with atherosclerosis (p < 0.001) and showed significant positive correlation with atherogenic lipid parameters (p < 0.001) and negative correlation with HDL-c (p < 0.05). Total ADA activity in atherosclerotic patients were statistically significantly higher than those in healthy donors (p < 0.001). There was statistically significant positive correlation between ADA activity and atherogenic lipid parameters. Significant negative correlation was found between ADA activity and protective HDL-c (p < 0.001). Activity of XO showed significant increase in serum of atherosclerotic patients (p < 0.001) compared to control group. There is also a significant positive correlation between XO and atherogenic lipids and significant negative correlation between XO and HDL-c. The obtained results suggest a possibility of purine metabolism enzymes estimation, using them as markers of endothelial damage in atherosclerosis, but also a potential of therapeutic correction in practice. 540 PREDICTIVE VALUE OF INFLAMMATORY MARKERS ON CARDIOVASCULAR EVENTS IN PATIENTS WITH CHRONIC HEART FAILURE S. Radovanovic1 , L. Stojanovic1 , M. Krotin1 , A. Djokovic1 , A. Savic-Radojevic2 , S.V. Dragan3 , T. Simic2 . 1 University Hospital Bezanijska Kosa, 2 Institute of Medical and Clinical Biochemistry, Faculty of Medicine, University of Belgrade, 3 Clinica of Cardiology, Clinical Center of Serbia, Belgrade, Serbia Background: With respect to clinical practice, recent prospective data indicate that markers of inflammation play a critical role in the progression of atherosclerosis and can be used to predict the future occurrence of cardiovascular events. However, it is unknown whether markers of inflamation in ishemic chronic heart failure (CHF) are truly independently associated with impaired outcome or progression of the disease.
Atherosclerosis Supplements 12, no. 1 (2011) 13–184
after further dividing CAD group using tHcy below 15 mM as reference, Trx activity was significantly decreased in CAD group, and was inversely associated with tHcy levels (r = −0.199, P < 0.05) that was, however, weakly positively associated with TrxR activity. Neither lipids nor glucose significantly affected Trx/TrxR activity. Association of CAD severity with low Trx plus high tHcy was strong (r = −0.458, P < 0.001), but with high tHcy alone was rather weak (r = 0.125, P = 0.225). Conclusion: In CAD patients, homocysteine-induced Trx falling appears to be involved in the disease severity. 536 THE EFFECT OF ROSUVASTATIN TREATMENT ON D-DIMER LEVELS IN OBESE PATIENTS WITH PRIMARY DYSLIPIDEMIA D.I. Agapakis1 , C. Savopoulos2 , K. Tziomalos2 , E.V. Massa2 , N. Katsiki2 , E. Satsoglou1 , A.I. Hatzitolios2 . 1 Internal Medicine, General Hospital of Goumenissa, Goumenissa, 2 First Propedeutic Department of Internal Medicine, Aristotle University of Thessaloniki, Hospital AHEPA, Thessalon´ıki, Greece Introduction: Risk of thromboembolic complications is increased in obese subjects. Current literature suggests that statin therapy decreases the risk of venous thrombosis and reduces plasma D-dimer (D-d) levels, a marker of hypercoagulable state. Aim: To evaluate the effect of rosuvastatin on D-d levels in patients with uncontrolled primary dyslipidemia and elevated body mass index (BMI). Material and Methods: 37 patients with uncontrolled primary dyslipidemia under hypolipidemic diet treatment, included in the study. The control group was 23 normolipidemic healthy subjects with similar characteristics (age, sex, BMI) with the study group. Dyslipidemic patients were treated with 10 mg/day rosuvastatin for 9 weeks. Lipids parameters and D-d levels (Triage D-Dimer test kit) were measured at baseline for both groups and after rosuvastatin treatment in dyslipidemic group. Results: At baseline, both groups had no significant differences in D-d levels (493±175 ng/ml vs 457±237 ng/ml, p > 0.05). In addition to lipid parameters, the D-d levels significantly decreased after rosuvastatin treatment from 493±175 ng/ml to 367±137 ng/ml, p < 0.001 Conclusions: These data support that beyond the hypolipidemic effect rosuvastatin also possesses significant anti-coagulation properties and could play a role in reducing thromboembolic complications in primary hyperlipidemic obese patients. 537 THE LINK BETWEEN CARDIAC AUTONOMIC FUNCTION AND MARKERS OF OXIDATIVE STRESS IN CORONAY ARTERY DISEASE (CAD) S. Radovanovic, L. Stojanovic, M. Zdravkovic, M. Krotin, B. Milovanovic. University Hospital Bezanijska Kosa, Belgrade, Serbia Background: The objective was to examine cardiovascular autonomic (cANS) function and its potential relationships with markers of oxidative stress in coronary artery disease (CAD) patients Methods: A total of 120 CAD patients mean age, 59±7 years (74 men and 46 women) were included in the stady. All of them had cANS function assessed via heart rate variability (HRV) methods during resting conditions. Standard time-domain and frequency-domain measures [high-frequency normalized units (HFnu; measure of parasympathetic nervous system activity) and low frequency:high-frequency ratio (LF:HF; overall sympathovagal balance)] of HRV were calculated. Plasma malondialdehyde (MDA), protein thiol (P-SH) and reactive carbonyl groups (RCD), together with glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities were determined spectrophotometrically and/or by ELISA methods and correlated with timedomein parameters of HRV (mean RR interval, SDNN, SDANN, rMSSD and pNN50%) as well as with frequency-domain measures (ULF, VLF, LF, LF/HF) Results: Significantly corelation was found between SDNN and GSH-Px (r = 0.221, p = 0.039) as well as between pNN50LF and MDA (r = 0.215, p = 0.045). By analyzing the corelation of frequency-domain measures with oxidative demage of proteins (P-SH) were observed following correlation: P-SH and ULF (r = −0.234, p = 0.034); P-SH and VLF (r = −0.219, p = 0.046); P-SH and LF/HF (r = −0.247, p = 0.025). LF was significantly related to GPx (r = 0.212, p = 0.054). Conclusion: We can conclude that cardiovascular autonomic (cANS) dysfunction assessed via heart rate variability was significantly related to increased oxidative stress in chronic heart failure.
Poster presentations
538 ISCHEMIA MODIFIED ALBUMIN (IMA) IN POSTMENOPAUSAL WOMEN WITH OR WITHOUT CORONARY ARTERY DISEASE (CAD) IN RELATION TO BMI AND OGTT K. Kazanis1 , M. Dalamaga1 , E. Kassi1 , G. Merantzi2 , I. Vagionas2 , G. Jullien2 , A. Dionyssiou-Asteriou1 . 1 Clinical Biochemistry, University of Athens, Medical School, 2 Laboratory of Biochemistry, Evangelismos General Hospital, Athens, Greece Background: IMA is a new biomarker of oxidative processes. Its measurement by the cobalt albumin binding assay is reported as an assessment of atherosclerotic burden. In postmenopausal women with or without CAD, we assessed IMA and determined its correlation with BMI, hsCRP and hyperglycaemia. Methods: 120 postmenopausal women, aged 43−80 years, were divided into three groups: Group A (n = 30) with BMI > 26−32 (15 with normal OGTT and 15 with impaired OGTT), Group B (n = 60) with BMI > 21−25 and Group C (n = 30) with BMI > 23−29 and documented CAD. Glucose, insulin, IMA, albumin and hsCRP were measured in an automated analyzer (Roche). The ratio IMA/albumin, HOMA and QUICKI were calculated. Results: IMA and IMA/albumin were significantly elevated in Group A compared to Group B (p < 0.001) but similar to those of Group C (p=1, p = 0.33 respectively). In Group A, there wasn’t any difference between subjects with normal and impaired OGTT. In Group A and B, serum hsCRP was similar but lower in comparison to Group C (p < 0.001). Glucose, insulin and HOMA were elevated in Group A compared to Group B while QUICKI was lower (p < 0.001). In Group A, IMA was positively correlated with BMI (p = 0.03), hsCRP (p < 0.001), insulin and HOMA and negatively with QUICKI (p < 0.01). Regression analysis revealed that obesity was the strongest predictor of IMA. Conclusions: Serum IMA in overweight women without CAD was similar to those with CAD. Whether IMA measurement provides an early assessment of atherosclerotic burden before clinical evidence of CAD needs further investigation. 539 DISORDERS OF PURINE METABOLISM ENZYMES IN ATHEROSCLEROSIS S. Kundalic1 , B. Kundalic2 , T. Ristic1 , D. Stankovic-Ferlez1 . 1 Centre of Medical Biochemistry, Clinical Centre Nis, 2 Faculty of Medicine, University of Nis, Nis, Serbia Since purine metabolism is important for endothelial cells’ normal function, purine catabolism enzymes have an especial place in damaged endothelium of atherosclerotic blood vessels in hyperlipidemias. The activities of 5’-nucleotidase (5’-NT), adenosine deaminase (ADA) and xanthine oxidase (XO) were investigated in atherosclerotic patients. This study obtained 55 patients with atherosclerosis whose results were compared to 46 healthy individuals. According to the values of lipid parameters: cholesterol, triglycerides, HDL-cholesterol (HDL-c) and LDL-cholesterol, the patients were allocated into hyperlipoproteinemic types by Fredrickson, while type subgroups were formed based on the values of hypercholesterolemia and hypertriglyceridemia. The most frequent types of hyperlipoproteinemias were IIa, IIb and type IV. Activity of 5’-NT was significantly increased in serum of patients with atherosclerosis (p < 0.001) and showed significant positive correlation with atherogenic lipid parameters (p < 0.001) and negative correlation with HDL-c (p < 0.05). Total ADA activity in atherosclerotic patients were statistically significantly higher than those in healthy donors (p < 0.001). There was statistically significant positive correlation between ADA activity and atherogenic lipid parameters. Significant negative correlation was found between ADA activity and protective HDL-c (p < 0.001). Activity of XO showed significant increase in serum of atherosclerotic patients (p < 0.001) compared to control group. There is also a significant positive correlation between XO and atherogenic lipids and significant negative correlation between XO and HDL-c. The obtained results suggest a possibility of purine metabolism enzymes estimation, using them as markers of endothelial damage in atherosclerosis, but also a potential of therapeutic correction in practice. 540 PREDICTIVE VALUE OF INFLAMMATORY MARKERS ON CARDIOVASCULAR EVENTS IN PATIENTS WITH CHRONIC HEART FAILURE S. Radovanovic1 , L. Stojanovic1 , M. Krotin1 , A. Djokovic1 , A. Savic-Radojevic2 , S.V. Dragan3 , T. Simic2 . 1 University Hospital Bezanijska Kosa, 2 Institute of Medical and Clinical Biochemistry, Faculty of Medicine, University of Belgrade, 3 Clinica of Cardiology, Clinical Center of Serbia, Belgrade, Serbia Background: With respect to clinical practice, recent prospective data indicate that markers of inflammation play a critical role in the progression of atherosclerosis and can be used to predict the future occurrence of cardiovascular events. However, it is unknown whether markers of inflamation in ishemic chronic heart failure (CHF) are truly independently associated with impaired outcome or progression of the disease.