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5395753 Method for diagnosing rheumatoid arthritis
PATENT ABSTRACTS
565
5393870
5395688
ANALOGS OF HUMAN GRANULOCYTE-MACROPHAGE COLONY STIMULATING FACTOR
MAGNETICALLY RESPONSIVE FLUORESCENT POLYMER PARTICLES
Deeley Michael C; Price Virginia L Seattle, WA, UNITED STATES Assigned to Immunex Corporation Amplified expression of recombinant DNA products is achieved in hosts expressing proteases that cleave at multi-basic amino acid residues. To this end, cDNAs encoding granulocytemacrophage colony stimulating factor (GM-CSF) are mutated such that one or both of the arginine residues at positions 23 and 24 of the protein product are deleted or replaced by non-basic amino acid residues. The GM-CSF analogs thus obtained maintain the activity of the wild-type protein.
Wang Chao-Huei J; Shah Dinesh Q Gurnee, IL, UNITED STATES Assigned to Baxter Diagnostics Inc This invention provides a novel process of producing magnetically responsive fluorescent polymer particles comprising polymeric core particles coated evenly with a layer of polymer containing magnetically responsive metal oxide. A wide variety of polymeric particles with sizes ranging from 1 to 100 microns can be used a core particles and transformed into magnetically responsive polymer particles. The sta'face of these magnetically responsive polymer particles can be coated further with another layer of functionalized polymer. These magnetically responsive fluorescent polymer particles can be used for passive or covalent coupling of biological material such as antigens, antibodies, enzymes or DNA/RNA hybridization and used as solid phase for various types of imrnunoassays, DNA/RNA hybridization probes assays, affinity purification, cell separation and other medical, diagnostic, and industrial applications.
5395591
5395750
APPARATUS OF IRRADIATING BIOLOGICAL SPECIMENS
METHODS FOR PRODUCING PROTEINS WHICH BIND TO PREDETERMINED ANTIGENS
Zimlich William C; Sorge Joseph A CA, 92121, UNITED STATES
San Diego,
A method of irradiating a biological specimen with ultraviolet, in particular a polynucleotide specimen selected from DNA or RNA, or optionally a protein. In the case where the specimen is DNA or RNA, or potentially proteins, the specimen is irradiated to cross-link the specimen to a subs~-ate. In the case where the specimen is DNA, the specimen can also be irradiated to form thymine dimers. The method uses an apparatus which permits relatively precise control of the total ultraviolet dose received by the specimen, despite any changes of ultraviolet flux from the lamps which may occur from during any one experiment, or between a number of experiments. Thus, the method allows relatively highly reproducible results to be obtained.
Dillon Patrick; Rosen Craig A Bloomfield, NJ, UNITED STATES Assigned to Hoffmann-La Roche Inc Methods of producing a protein capable of binding to a predetermined antigen, screening libraries containing such proteins, and proteins and synthetic genes containing randomized sequences are disclosed.
5395753 METHOD FOR DIAGNOSING RHEUMATOID ARTHRITIS Prakash Ramesh Salt Lake City, UT, UNITED STATES Assigned to Theratech Inc
566
PATENT ABSTRACTS
A method is described for diagnosing rheumatoid arthritis by providing a recombinant IgM-specific rheumatoid arthritis-associated antigen and detecting antibodies against the antigen in patient sera. Preliminary steps of making a cDNA library from polyadenylated RNA purified from human ceils, selecting recombinants that express the antigen, recloning cDNA containing the antigen gene in a high level expression vector, expressing the antigen in transformed cells, and purifying the antigen are also described.
5395756 PRODUCTION OF ACIDIC FGF PROTEIN Igarashi Koich Kyoto, JAPAN Assigned to Takeda Chemical Industries Ltd An expression vector comprises a cDNA sequence encoding an aFGF protein and a T7 promoter upstream therefrom. The vector is useful for transforming host cells and expressing the aFGF gene.
5395759 DNA SEQUENCE AND AMINO ACID SEQUENCE ENCODING THE HUMAN ROTAVIRUS MAJOR OUTER CAPSID GLYCOPROTEIN Holmes lan H; Dyall-Smith Michael L Canterbury, AUSTRALIA Assigned to The University of Melbourne PCT No. PCT/AU85/00096 Sec. 371 Date Feb. 4, 1987 Sec. 102(e) Date Feb. 4, 1987 PCT Filed Apr. 29, 1985 PCT Pub. No. WO85/05122 PCT Pub. Date Nov. 21, 1985. A material being a dsRNA gene segment coding for the major outer capsid glycoprotein of a rotavirus.
5395760 DNA ENCODING TUMOR NECROSIS FACTOR- ALPHA AND - BETA RECEPTORS Smith Craig A; Goodwin Raymond; Beckmann M Patrici Seattle, WA, UNITED STATES Assigned to Immunex Corporation
Tumor necrosis factor receptor proteins, DNAs and expression vectors encoding TNF receptors, and processes for producing TNF receptors as products of recombinant cell culture, are disclosed.
5395764 PROMOTER OF THE GENE WHICH CODES FOR THE PILINIC SUBUNIT FIM3 OF BORDETELLA PERTUSSIS AND ITS USE FOR THE EXPRESSION IN BORDETELLA OF THE GENES WHICH CODE FOR A PROTEIN OF INTEREST Riboli Barbara; Pedroni Paola; Cuzzoni Anna; de Ferra Francesca; Grandi Guido Cremona, ITALY Assigned to Eniricerhce S p A The isolation and characterization of the promoter regions of the genes which code for the pilinic subunits tim2, tim3 and fimx of Bordetella pertussis, are described, as well as the construction of vectors containing the regions, and microorganisms transformed by the vectors. The promoter regions, or nucleotide fragments thereof, are particularly useful for the regulable or nonregulable expression of genes which code for a protein of interest in a strain of Bordetella. The transformed Bordetella strains are particularly suitable for the development of an effective antipertussis vaccine.
5395765 THERMOSTABLE XYLANASE FROM A STRAIN OF RHODOTHERMUS MARINUS Dahlberg Leif A L; Hoist Olof P; Anker Lisbeth SWEDEN Assigned to Novo Nordisk A/S PCT No. PCT/DK92/00300 Sec. 371 Date Mar. 22, 1994 Sec. 102(e) Date Mar. 22, 1994 PCT Filed Oct. 14, 1992 PCT Pub. No. WO93/08275 PCT Pub. Date Apr. 29, 1993. A xylanase obtained from Rhodothermus marinus, the method of obtaining it and a process for its use are disclosed. The xylanase has an optimum temperature of from 85 degrees to 100 degrees C., a relative activity of more than 50% in the interval of from pH 5 tp pH 8 after incubation for 5 minutes at 65 degrees C., a relative temperature stability at 80 degrees C. of
565
5393870
5395688
ANALOGS OF HUMAN GRANULOCYTE-MACROPHAGE COLONY STIMULATING FACTOR
MAGNETICALLY RESPONSIVE FLUORESCENT POLYMER PARTICLES
Deeley Michael C; Price Virginia L Seattle, WA, UNITED STATES Assigned to Immunex Corporation Amplified expression of recombinant DNA products is achieved in hosts expressing proteases that cleave at multi-basic amino acid residues. To this end, cDNAs encoding granulocytemacrophage colony stimulating factor (GM-CSF) are mutated such that one or both of the arginine residues at positions 23 and 24 of the protein product are deleted or replaced by non-basic amino acid residues. The GM-CSF analogs thus obtained maintain the activity of the wild-type protein.
Wang Chao-Huei J; Shah Dinesh Q Gurnee, IL, UNITED STATES Assigned to Baxter Diagnostics Inc This invention provides a novel process of producing magnetically responsive fluorescent polymer particles comprising polymeric core particles coated evenly with a layer of polymer containing magnetically responsive metal oxide. A wide variety of polymeric particles with sizes ranging from 1 to 100 microns can be used a core particles and transformed into magnetically responsive polymer particles. The sta'face of these magnetically responsive polymer particles can be coated further with another layer of functionalized polymer. These magnetically responsive fluorescent polymer particles can be used for passive or covalent coupling of biological material such as antigens, antibodies, enzymes or DNA/RNA hybridization and used as solid phase for various types of imrnunoassays, DNA/RNA hybridization probes assays, affinity purification, cell separation and other medical, diagnostic, and industrial applications.
5395591
5395750
APPARATUS OF IRRADIATING BIOLOGICAL SPECIMENS
METHODS FOR PRODUCING PROTEINS WHICH BIND TO PREDETERMINED ANTIGENS
Zimlich William C; Sorge Joseph A CA, 92121, UNITED STATES
San Diego,
A method of irradiating a biological specimen with ultraviolet, in particular a polynucleotide specimen selected from DNA or RNA, or optionally a protein. In the case where the specimen is DNA or RNA, or potentially proteins, the specimen is irradiated to cross-link the specimen to a subs~-ate. In the case where the specimen is DNA, the specimen can also be irradiated to form thymine dimers. The method uses an apparatus which permits relatively precise control of the total ultraviolet dose received by the specimen, despite any changes of ultraviolet flux from the lamps which may occur from during any one experiment, or between a number of experiments. Thus, the method allows relatively highly reproducible results to be obtained.
Dillon Patrick; Rosen Craig A Bloomfield, NJ, UNITED STATES Assigned to Hoffmann-La Roche Inc Methods of producing a protein capable of binding to a predetermined antigen, screening libraries containing such proteins, and proteins and synthetic genes containing randomized sequences are disclosed.
5395753 METHOD FOR DIAGNOSING RHEUMATOID ARTHRITIS Prakash Ramesh Salt Lake City, UT, UNITED STATES Assigned to Theratech Inc
566
PATENT ABSTRACTS
A method is described for diagnosing rheumatoid arthritis by providing a recombinant IgM-specific rheumatoid arthritis-associated antigen and detecting antibodies against the antigen in patient sera. Preliminary steps of making a cDNA library from polyadenylated RNA purified from human ceils, selecting recombinants that express the antigen, recloning cDNA containing the antigen gene in a high level expression vector, expressing the antigen in transformed cells, and purifying the antigen are also described.
5395756 PRODUCTION OF ACIDIC FGF PROTEIN Igarashi Koich Kyoto, JAPAN Assigned to Takeda Chemical Industries Ltd An expression vector comprises a cDNA sequence encoding an aFGF protein and a T7 promoter upstream therefrom. The vector is useful for transforming host cells and expressing the aFGF gene.
5395759 DNA SEQUENCE AND AMINO ACID SEQUENCE ENCODING THE HUMAN ROTAVIRUS MAJOR OUTER CAPSID GLYCOPROTEIN Holmes lan H; Dyall-Smith Michael L Canterbury, AUSTRALIA Assigned to The University of Melbourne PCT No. PCT/AU85/00096 Sec. 371 Date Feb. 4, 1987 Sec. 102(e) Date Feb. 4, 1987 PCT Filed Apr. 29, 1985 PCT Pub. No. WO85/05122 PCT Pub. Date Nov. 21, 1985. A material being a dsRNA gene segment coding for the major outer capsid glycoprotein of a rotavirus.
5395760 DNA ENCODING TUMOR NECROSIS FACTOR- ALPHA AND - BETA RECEPTORS Smith Craig A; Goodwin Raymond; Beckmann M Patrici Seattle, WA, UNITED STATES Assigned to Immunex Corporation
Tumor necrosis factor receptor proteins, DNAs and expression vectors encoding TNF receptors, and processes for producing TNF receptors as products of recombinant cell culture, are disclosed.
5395764 PROMOTER OF THE GENE WHICH CODES FOR THE PILINIC SUBUNIT FIM3 OF BORDETELLA PERTUSSIS AND ITS USE FOR THE EXPRESSION IN BORDETELLA OF THE GENES WHICH CODE FOR A PROTEIN OF INTEREST Riboli Barbara; Pedroni Paola; Cuzzoni Anna; de Ferra Francesca; Grandi Guido Cremona, ITALY Assigned to Eniricerhce S p A The isolation and characterization of the promoter regions of the genes which code for the pilinic subunits tim2, tim3 and fimx of Bordetella pertussis, are described, as well as the construction of vectors containing the regions, and microorganisms transformed by the vectors. The promoter regions, or nucleotide fragments thereof, are particularly useful for the regulable or nonregulable expression of genes which code for a protein of interest in a strain of Bordetella. The transformed Bordetella strains are particularly suitable for the development of an effective antipertussis vaccine.
5395765 THERMOSTABLE XYLANASE FROM A STRAIN OF RHODOTHERMUS MARINUS Dahlberg Leif A L; Hoist Olof P; Anker Lisbeth SWEDEN Assigned to Novo Nordisk A/S PCT No. PCT/DK92/00300 Sec. 371 Date Mar. 22, 1994 Sec. 102(e) Date Mar. 22, 1994 PCT Filed Oct. 14, 1992 PCT Pub. No. WO93/08275 PCT Pub. Date Apr. 29, 1993. A xylanase obtained from Rhodothermus marinus, the method of obtaining it and a process for its use are disclosed. The xylanase has an optimum temperature of from 85 degrees to 100 degrees C., a relative activity of more than 50% in the interval of from pH 5 tp pH 8 after incubation for 5 minutes at 65 degrees C., a relative temperature stability at 80 degrees C. of