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5399283 Thermally stable and PH stable subtilisin analogs and method for production thereof
P A T E N T ABSTRACTS
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presence of a plant host, comprising mutating a bacterial culture with a transposon containing a promoterless marker gene, exposing the bacterial mutants to root exudates, other plant-derived substances or plants, and identifying mutants that have a transposon-associated gene inducible by the plant. These mutants may be used to clone and characterize the promoters and the associated wild-type bacterial genes responsive to the plant.
5397698 AMPLIFICATION METHOD FOR POLYNUCLEOTIDE DETECTION ASSAYS Goodman Thomas C; Becker Marti; Ullman Edwin; Rose Samue Mountain View, CA, UNITED STATES Assigned to Syntex (U S A ) Inc A method is disclosed for producing multiple copies of a primary polynucleotidesequence located at the 3' terminus of a polynucleotide. The method comprises (a) forming in the presence of nucleoside triphosphates and template-dependent polynucleotide polymerase an extension of a primary polynucleotide sequence hybridized with a template sequence of a single stranded pattern polynucleotide comprising two or more template sequences each containing one or more site specific cleavage sequences, (b) cleaving into fragments said extension at cleavable polynucleotide sequences in the presence of means for specifically cleaving said cleavable polynucleotide sequences when said extension is hybridized with said site specific cleavage sequences, (c) dissociating said fragments, (d) hybridizing said fragments with single stranded pattern polynucleotide, and repeating steps (a)-(d). Steps (a)-(d) may be conducted simultaneously or wholly or partially sequentially. The method may be applied in the detection of a polynucleotide analyte in a sample suspected of containing such analyte to facilitate such detection. Also disclosed are compositions for conducting the method of the invention.
5397705 MULTIPLY MUTATED SUBTILISINS Zukowski Mark; Narhi Linda O; Levitt Michael Thousand Oaks, CA, UNITED STATES Assigned to Amgen Inc
A class of subtilisin analogs suitable for admixture to cleaning compositions and having improved stability over naturally occurring Bacillus subtilisins are prepared by expressing a modified gene encoding the subtilisin analog in Bacillus subtilis.
~9~83 THERMALLY STABLE AND PH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF Stabinsky Yitzhak; Zukowski Mark M Boulder, CO, UNITED STATES Assigned to Amgen Inc A mutated subtihsin suitable for admixture to washing compositions and exhibiting substantially improved stability over naturally occurring Bacillus serine proteases is prepared by expressing a modified gene encoding subtilisin in Bacillus subtilis. A preferred subtilisin analog product differs from wild-type Bacillus alkaline proteases by having any amino acid, and preferably serine, at position 218 in place of nsparagine. The product is preferably produced in a strain of B. subtilis which is mutated to block synthesis of endogenous proteases. The method of replacing an Ash or a Gly in an Asn-Oly sequence in order to improve pH and thermal stability may be applied to other sites in subtilisin and to other proteins as well.
5399346 GENE THERAPY Anderson W French; Blaese R Michael; Rosenberg Steven A Bethesda, MD, UNITED STATES Assigned to The United States of America as represented by the Department of Health and Human Services Primary human cells which are genetically engineered with DNA (RNA) encoding a marker or therapeutic which is expressed to be expressed in vivo. Such engineered cells may be used in gene therapy.
568
presence of a plant host, comprising mutating a bacterial culture with a transposon containing a promoterless marker gene, exposing the bacterial mutants to root exudates, other plant-derived substances or plants, and identifying mutants that have a transposon-associated gene inducible by the plant. These mutants may be used to clone and characterize the promoters and the associated wild-type bacterial genes responsive to the plant.
5397698 AMPLIFICATION METHOD FOR POLYNUCLEOTIDE DETECTION ASSAYS Goodman Thomas C; Becker Marti; Ullman Edwin; Rose Samue Mountain View, CA, UNITED STATES Assigned to Syntex (U S A ) Inc A method is disclosed for producing multiple copies of a primary polynucleotidesequence located at the 3' terminus of a polynucleotide. The method comprises (a) forming in the presence of nucleoside triphosphates and template-dependent polynucleotide polymerase an extension of a primary polynucleotide sequence hybridized with a template sequence of a single stranded pattern polynucleotide comprising two or more template sequences each containing one or more site specific cleavage sequences, (b) cleaving into fragments said extension at cleavable polynucleotide sequences in the presence of means for specifically cleaving said cleavable polynucleotide sequences when said extension is hybridized with said site specific cleavage sequences, (c) dissociating said fragments, (d) hybridizing said fragments with single stranded pattern polynucleotide, and repeating steps (a)-(d). Steps (a)-(d) may be conducted simultaneously or wholly or partially sequentially. The method may be applied in the detection of a polynucleotide analyte in a sample suspected of containing such analyte to facilitate such detection. Also disclosed are compositions for conducting the method of the invention.
5397705 MULTIPLY MUTATED SUBTILISINS Zukowski Mark; Narhi Linda O; Levitt Michael Thousand Oaks, CA, UNITED STATES Assigned to Amgen Inc
A class of subtilisin analogs suitable for admixture to cleaning compositions and having improved stability over naturally occurring Bacillus subtilisins are prepared by expressing a modified gene encoding the subtilisin analog in Bacillus subtilis.
~9~83 THERMALLY STABLE AND PH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF Stabinsky Yitzhak; Zukowski Mark M Boulder, CO, UNITED STATES Assigned to Amgen Inc A mutated subtihsin suitable for admixture to washing compositions and exhibiting substantially improved stability over naturally occurring Bacillus serine proteases is prepared by expressing a modified gene encoding subtilisin in Bacillus subtilis. A preferred subtilisin analog product differs from wild-type Bacillus alkaline proteases by having any amino acid, and preferably serine, at position 218 in place of nsparagine. The product is preferably produced in a strain of B. subtilis which is mutated to block synthesis of endogenous proteases. The method of replacing an Ash or a Gly in an Asn-Oly sequence in order to improve pH and thermal stability may be applied to other sites in subtilisin and to other proteins as well.
5399346 GENE THERAPY Anderson W French; Blaese R Michael; Rosenberg Steven A Bethesda, MD, UNITED STATES Assigned to The United States of America as represented by the Department of Health and Human Services Primary human cells which are genetically engineered with DNA (RNA) encoding a marker or therapeutic which is expressed to be expressed in vivo. Such engineered cells may be used in gene therapy.