5422259 Transgenic plants belonging to the species Cucumis melo

5422259 Transgenic plants belonging to the species Cucumis melo

388 PATENT ABSTRACTS 5422259 5424409 TRANSGENIC PLANTS BELONGING TO THE SPECIES CUCUMIS MELO DNA CONSTRUCTS ENCODING BACILLUS THURINGIENSIS TOXIN...

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388

PATENT ABSTRACTS

5422259

5424409

TRANSGENIC PLANTS BELONGING TO THE SPECIES CUCUMIS MELO

DNA CONSTRUCTS ENCODING BACILLUS THURINGIENSIS TOXINS FROM STRAIN A20

De Both Michiel; Ben Tahar Sophia: Noel Marianne; Perret Joel Beaumont, FRANCE Assigned to Biosem

Ely Susan: Tippett Janet M Bucks, UNITED KINGDOM Assigned to Imperial Chemical Industries PLC

Procedure for the production of transgenic seedlings starting from genetically transformed buds, the said seedlings belonging to the species Cucumus melo and containing at least one gene introduced through the intermediary of Agrobacterium tumefaciens, characterized by the culture in two successive stages of genetically transformed buds, the first of these steps taking place in a plant cell culture medium containing a cytokinin and more particularly 6-benzyl aminopurine (BAP), and the second, which is performed when the buds have attained a height of about at least 3 mm, taking place in a plant cell culture medium containing as macro-elements: (*See Patent for Tabular Presentation*)

PCT No. PCT/GB89/01157 Sec. 371 Date Apr. 1, 1991 Sec. 102(e) Date Apr. 1, 1991 PCT Filed Sep. 29, 1989 PCT Pub. No. WO90/03434 PCT Pub. Date Apr. 5, 1990. Recombinant DNA coding for an insecticidally active form of the Bacillus thuringiensis endotoxin which DNA is derived from the chromosome plasmids of the A20 strain of Bacillus thuringiensis. The recombinant genes may be of the 6.6, 5.3 or 4.5 types. Further discloses a truncated chimetic endotoxin-producing gene derived from the 5.3 and 4.5-type genes of strain A20.

5424398 PEPTIDES AND NUCLEIC ACID SEQUENCES RELATED TO THE EPSTEIN BARR VIRUS Middeldorp Jaap M; van Grunsven Wouterus M J Oss, NETHERLANDS Assigned to Akzo N V The present invention relates to peptides immunochemically reactive with antibodies to the Epstein-Barr virus (EBV), comprising at least part of the VCA-pl8 or VCA-p40 protein, encoded within the EBV open reading frames BFRF3 and BdRF1 respectively, or a functional variant thereof. The invention further relates to nucleic acid sequences encoding these peptides, monoclonal antibodies against these peptides, cell lines capable of producing monoclonal antibodies and anti-idiotype antibodies. The invention also relates to recombinant vector molecules comprising a nucleic acid sequence according to the invention and host cells transformed or transfected with these vector molecules. The invention is further concerned with immunological reagents and methods for the detection of EBV or anti-EBV antibodies and a method for the amplification and detection of Epstein Barr viral nucleic acid.

5424410 BACILLUS THURINGIENSIS ISOLATES FOR CONTROLLING ACARIDES Payne Jewel; Cannon Raymond J; Bagley Angela L San Diego, CA, UNITED STATES Assigned to Mycogen Corporation Disclosed and claimed are Bacillus thuringiensis isolates designated B.t. PS50C, B.t. PS86A1, B.t. PS69D1, B.t. PS72LI, B.t. PS75JI, B.t. PS83E5, B.t. PS45B1, B.t.. PS24J, B.t. PS94R3, B.t. PS17, B.t. PS62B1 and B.t. PS74G1 which are active against acaride pests. Thus, these isolates, or mutants thereof, can be used to control such pests. Further, genes encoding novel delta-endotoxins can be removed from these isolates and transferred to other host microbes, or plants. Expression of the delta-endotoxins in microbe hosts results in the control of acaride pests, whereas transformed plants become resistant to acaride pests.