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5424191 Epithelial cell specific differentiation marker
306
PATENT ABSTRACTS
5424191
space to intracellular domains. The physiological, kinetic and pharmacological characteristics of transport in these cells conform to known characteristics of high-affinity neurotransmitter transport in the CNS.
EPITHELIAL CELL SPECIFIC DIFFERENTIATION M A R K E R
5424186
Prasad Gaddamanugu; Cooper Herbert Rockville, MD, UNITED STATES Assigned to The United States of America as represented by the Department of Health and Human Services
VERY L A R G E SCALE IMMOBILIZED POLYMER SYNTHESIS Fodor Stephen P; Stryer Lubert; Pirrung Michael; Read J Leighton Palo Alto, CA, UNITED STATES Assigned to Affymax Technologies N V A method for synthesizing oligonucleotides on a solid substrate. The method provides for the irradiation of a first predefined region of the substrate without irradiation of a first predefined region of the substrate. The irradiation of a second predefined region of the substrate. The irradiation step removes a protecting group therefrom. The substrate is contacted with a first nucleotide to couple the nucleotide to the substrate in the first predefined region. By repeating these steps, an array of diverse oligonucleotides is formed on the substrate.
5424188 AMPLIFIED H Y B R I D I Z A T I O N ASSAY Schneider Robert; Shenk Thomas New York, NY, UNITED STATES Assigned to The Trustees of Princeton University A kit for an amplified hybridization assay is described in which a family of signal-generating secondary probes bind to a primary probe that hybridizes to the target sequence of interest. Thus, an enormously amplified signal is generated by the hybridization event. The assay can be used for a variety of laboratory and clinical purposes and is automatable. A hybridization assay kit is also described. The kit is used for the detection of a target nucleotide sequence. One embodiment of the kit includes a plurality of secondary probes, each secondary probe capable of binding to a distinct binding site of the primary probe.
A full length cDNA clone from a normal human mammary epithelial cell (strain 184) encoding a 25 kDa protein, HME1, was isolated. Expression of HME1 RNA appears to be limited to epithelial cells. The HMEI sequence shares strong sequence homology with bovine 14-3-3 protein, which is an activator of tyrosine and tryptophan hydroxylase, but the tissue distribution of HMEI differs from that of 14-3-3. Compared with normal mammary epithelial cells, expression of HME1 RNA is dramatically low in cells derived from human mammary carcinoma and in normal mammary epithelial cells transformed by oncogenes. HME1 therefore appears to be a cellular differentiation marker that is down-regulated during neoplastic transformation.
5424198 H U M A N TPA P R O D U C T I O N USING V E C T O R S CODING FOR DHFR PROTEIN Levinson Arthur; Pennica Diane; Kohr William J; Vehar Gordon A; Goeddel David V; Yelverton Elizabeth M; Simonsen Christian C Hillsborough, CA, UNITED STATES Assigned to Genentech Inc A method for producing tissue plasminogen activator (t-PA)in eukaroytic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild-type DHFR with methotrexate.
5424199 HUMAN GROWTH HORMONE Goeddel David V; Heyneker Herbert Burlingame, CA, UNITED STATES Assigned to Genentech lnc
PATENT ABSTRACTS
5424191
space to intracellular domains. The physiological, kinetic and pharmacological characteristics of transport in these cells conform to known characteristics of high-affinity neurotransmitter transport in the CNS.
EPITHELIAL CELL SPECIFIC DIFFERENTIATION M A R K E R
5424186
Prasad Gaddamanugu; Cooper Herbert Rockville, MD, UNITED STATES Assigned to The United States of America as represented by the Department of Health and Human Services
VERY L A R G E SCALE IMMOBILIZED POLYMER SYNTHESIS Fodor Stephen P; Stryer Lubert; Pirrung Michael; Read J Leighton Palo Alto, CA, UNITED STATES Assigned to Affymax Technologies N V A method for synthesizing oligonucleotides on a solid substrate. The method provides for the irradiation of a first predefined region of the substrate without irradiation of a first predefined region of the substrate. The irradiation of a second predefined region of the substrate. The irradiation step removes a protecting group therefrom. The substrate is contacted with a first nucleotide to couple the nucleotide to the substrate in the first predefined region. By repeating these steps, an array of diverse oligonucleotides is formed on the substrate.
5424188 AMPLIFIED H Y B R I D I Z A T I O N ASSAY Schneider Robert; Shenk Thomas New York, NY, UNITED STATES Assigned to The Trustees of Princeton University A kit for an amplified hybridization assay is described in which a family of signal-generating secondary probes bind to a primary probe that hybridizes to the target sequence of interest. Thus, an enormously amplified signal is generated by the hybridization event. The assay can be used for a variety of laboratory and clinical purposes and is automatable. A hybridization assay kit is also described. The kit is used for the detection of a target nucleotide sequence. One embodiment of the kit includes a plurality of secondary probes, each secondary probe capable of binding to a distinct binding site of the primary probe.
A full length cDNA clone from a normal human mammary epithelial cell (strain 184) encoding a 25 kDa protein, HME1, was isolated. Expression of HME1 RNA appears to be limited to epithelial cells. The HMEI sequence shares strong sequence homology with bovine 14-3-3 protein, which is an activator of tyrosine and tryptophan hydroxylase, but the tissue distribution of HMEI differs from that of 14-3-3. Compared with normal mammary epithelial cells, expression of HME1 RNA is dramatically low in cells derived from human mammary carcinoma and in normal mammary epithelial cells transformed by oncogenes. HME1 therefore appears to be a cellular differentiation marker that is down-regulated during neoplastic transformation.
5424198 H U M A N TPA P R O D U C T I O N USING V E C T O R S CODING FOR DHFR PROTEIN Levinson Arthur; Pennica Diane; Kohr William J; Vehar Gordon A; Goeddel David V; Yelverton Elizabeth M; Simonsen Christian C Hillsborough, CA, UNITED STATES Assigned to Genentech Inc A method for producing tissue plasminogen activator (t-PA)in eukaroytic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild-type DHFR with methotrexate.
5424199 HUMAN GROWTH HORMONE Goeddel David V; Heyneker Herbert Burlingame, CA, UNITED STATES Assigned to Genentech lnc