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5427786 Bacillus thuringiensis isolates selectively active against certain coleopteran pests
PATENT ABSTRACTS
nucleic acid hybridization or immunoassay, a method for identifying a compound capable of inducing the expression of TSG-14 in a cell, and a method for measuring the ability of a cell to respond to TNF are also provided.
5427786 BACILLUS THURINGIENSIS ISOLATES SELECTIVELY ACTIVE AGAINST CERTAIN COLEOPTERAN PESTS Payne Jewel M; Michaels Tracy San Diego, CA, UNITED STATES Assigned to Mycogen Corporation The subject invention concerns Bacillus thuringiensis microbes with activity against select coleopteran pests e.g., Diabrotica sp., Hypera sp., and various flea beetles, For example, the B.t. isolates of the invention are active against alfalfa weevils-AW (Hypera brunneipennis), rape flea beetles-RFB (Phyllotreta cruciferae) and corn rootworms-CRW (Diabrotica undecimpunctata undecimpunctata). Thus, these microbes can be used to control these pests, Further, genes encoding toxins active against these pests can be isolated from the B.t. isolates and used to transform other microbes. The transformed microbes then can be used to control susceptible coleopteran pests.
5427788 PERTUSSIS
TOXIN AND USE IN VACCINES
Rappuoli Rino; Nicosia Alfredo;Arico'Maria B Quercegrossa Monteriggioni, ITALY Assigned to Sclavo S p A Cloning and sequencing of the Eco RI fragment of B. pertussis chromosomal DNA with 4696 base pairs, containing the genes which code for the five subunits of the pertussis toxin. A hybrid plasmid containing the DNA fragment or its further fragments and a micro-organism transformed by the hybrid plasmid and capable of expressing the cloned DNA fragment or further fragments thereof by synthesis of the pertussis toxin or one or more subunits of the pertussis toxin. The pertussis toxin or one or more subunits of the pertussis toxin so obtained are useful for the preparation of vaccines and diagnostic kits.
313
5427908 RECOMBINANT LIBRARY SCREENING METHODS Dower William J; Cwida Steven E Menlo Park, CA, UNITED STATES Assigned to Affymax Technologies N V Nucleotide sequences encoding proteins of interest are isolated from DNA libraries using bacteriophage to link the protein to the sequence which encodes it. DNA libraries are prepared from cells encoding the protein of interest and inserted into or adjacent to a coat protein of a bacteriophage vector, or into a sequence encoding a protein which may be linked by means of a ligand to a phage coat protein. By employing affinity purification techniques the phage particles containing sequences encoding the desired protein may be selected and the desired nucleotide sequences obtained therefrom. Thus, for example, novel proteins such as monoclonal antibodies may be produced and conventional hybridoma technology avoided.
5427911 COUPLED AMPLIFICATION AND SEQUENCING OF DNA Ruano Gualbert New Haven, CT, UNITED STATES Assigned to Yale University A process for sequencing DNA segments hid aving complementary strands comprising (a) synthesizing simultaneously truncated products and full-length products starting from both 3' ends of the complementary strands of the DNA segment, which serves as a template, by introducing specific oligonucleotide primers annealing to the 3' ends of both complementary strands of the DNA segment, a deoxyribonucleotide elongator for each of adenine, guanine, cytosine and thymine, a thermally stable DNA polymerase and a terminator for each of adenine, guanine, cytosine and thymine, (b) thermally cycling step (a), (c) separating out the resultant truncated products and full length products according to size, and (d) selectively detecting all truncated products and full length products according to size, and (e) selectively detecting all truncated products and full-length products generated from a given strand of template.
nucleic acid hybridization or immunoassay, a method for identifying a compound capable of inducing the expression of TSG-14 in a cell, and a method for measuring the ability of a cell to respond to TNF are also provided.
5427786 BACILLUS THURINGIENSIS ISOLATES SELECTIVELY ACTIVE AGAINST CERTAIN COLEOPTERAN PESTS Payne Jewel M; Michaels Tracy San Diego, CA, UNITED STATES Assigned to Mycogen Corporation The subject invention concerns Bacillus thuringiensis microbes with activity against select coleopteran pests e.g., Diabrotica sp., Hypera sp., and various flea beetles, For example, the B.t. isolates of the invention are active against alfalfa weevils-AW (Hypera brunneipennis), rape flea beetles-RFB (Phyllotreta cruciferae) and corn rootworms-CRW (Diabrotica undecimpunctata undecimpunctata). Thus, these microbes can be used to control these pests, Further, genes encoding toxins active against these pests can be isolated from the B.t. isolates and used to transform other microbes. The transformed microbes then can be used to control susceptible coleopteran pests.
5427788 PERTUSSIS
TOXIN AND USE IN VACCINES
Rappuoli Rino; Nicosia Alfredo;Arico'Maria B Quercegrossa Monteriggioni, ITALY Assigned to Sclavo S p A Cloning and sequencing of the Eco RI fragment of B. pertussis chromosomal DNA with 4696 base pairs, containing the genes which code for the five subunits of the pertussis toxin. A hybrid plasmid containing the DNA fragment or its further fragments and a micro-organism transformed by the hybrid plasmid and capable of expressing the cloned DNA fragment or further fragments thereof by synthesis of the pertussis toxin or one or more subunits of the pertussis toxin. The pertussis toxin or one or more subunits of the pertussis toxin so obtained are useful for the preparation of vaccines and diagnostic kits.
313
5427908 RECOMBINANT LIBRARY SCREENING METHODS Dower William J; Cwida Steven E Menlo Park, CA, UNITED STATES Assigned to Affymax Technologies N V Nucleotide sequences encoding proteins of interest are isolated from DNA libraries using bacteriophage to link the protein to the sequence which encodes it. DNA libraries are prepared from cells encoding the protein of interest and inserted into or adjacent to a coat protein of a bacteriophage vector, or into a sequence encoding a protein which may be linked by means of a ligand to a phage coat protein. By employing affinity purification techniques the phage particles containing sequences encoding the desired protein may be selected and the desired nucleotide sequences obtained therefrom. Thus, for example, novel proteins such as monoclonal antibodies may be produced and conventional hybridoma technology avoided.
5427911 COUPLED AMPLIFICATION AND SEQUENCING OF DNA Ruano Gualbert New Haven, CT, UNITED STATES Assigned to Yale University A process for sequencing DNA segments hid aving complementary strands comprising (a) synthesizing simultaneously truncated products and full-length products starting from both 3' ends of the complementary strands of the DNA segment, which serves as a template, by introducing specific oligonucleotide primers annealing to the 3' ends of both complementary strands of the DNA segment, a deoxyribonucleotide elongator for each of adenine, guanine, cytosine and thymine, a thermally stable DNA polymerase and a terminator for each of adenine, guanine, cytosine and thymine, (b) thermally cycling step (a), (c) separating out the resultant truncated products and full length products according to size, and (d) selectively detecting all truncated products and full length products according to size, and (e) selectively detecting all truncated products and full-length products generated from a given strand of template.