- Email: [email protected]
5432059 Assay for glycosylation deficiency disorders
PATENT ABSTRACTS
5432055 DETECTION OF PORPHYROMONAS GINGIVALIS Evans Mary J; Evans Richard T; G-enco Robert J; Crreenberg Steven; Kuramitsu Howard East Amherst, NY, UNITED STATES The present invention relates to novel compositions comprising P. gingivalis specific oligonucleotides which axe useful as primers to amplify particular regions of the genome of P. gingivalis during enzymatic nucleic acid amplification. The invention also provides a method for the detection of P. gingivalis, which may be present in a clinical specimen, using the P. gingivalis-specific primers and enzymatic nucleic acid amplification. The present invention also relates to P. gingivalis-specific oligonucleotides which are useful as probes to facilitate detection of the amplified regions of P. gingivalis DNA.
5432057 SIMULTANEOUS CALIBRATION HETEROGENEOUS IMMUNOASSAY Litman David J; Ullman Edwin F Mountain View, CA, UNITED STATES assigned to Syva Company An assay method and compositions are provided for determining the presence of an analyte in a sample. The analyte is a member of an immunological pair (mip) comprising ligand and receptor. By providing a first measurement surface capable of specifically binding a labelled reagent in an amount depending upon the presence of analyte in the sample and a second calibration surface capable of binding a second labeled reagent in a manner unaffected by the presence of analyte in the sample, calibration of individual tests can be accomplished simultaneously with the performance of the test itself. A signal producing system includes an enzyme, a catalyst usually bonded to a mip which defines the first labeled reagent for binding to the measurement surface and the same
491
catalyst conjugated to a ligand capable of binding to the calibration surface. Preferably, both labeled reagents have the same composition and the calibration surface includes anti-(first catalyst).
5432058 METHOD
FOR MEASURING
HUMAN CHOLESTEROL ABSORPTION USING METABOLICALLY STABLE ISOTOPES Lange Louis; Ostlund Richard; Bosner Matthew Portola Valley, CA, 94028, UNITED STATES This invention is a method for measuring the ability of a human to absorb cholesterol. The method uses two different cholesterol Iracers, the first injected into the blood stream and the second ingested by the human subject. Atler a waiting period a blood sample is taken from the human subject and analyzed to determine percent cholesterol absorption based on the actual amounts of the two naturally occurring, metabolically stable cholesterol tracers in the blood. The method of this invention is useful in identifying human subjects as high cholesterol absorbers and thereafter administering therapeutic agents to the subject that inhibit the absorption of cholesterol.
5432059 ASSAY FOR GLYCOSYLATION DEFICIENCY DISORDERS Bean Pamela; Terryberry Jeff Los Angeles, CA, UNITED STATES assigned to Specialty Laboratories lnc A method is provided for detecting carbohydrate-deficient glycoproteins in samples taken from subjects with metabolic disorders, such as alcohol abuse and subjects who display a syndrome of carrying abnormal levels of carbohydrate deficient glycoproteins. The method involves steps of reglycosylating with a fluorescent-conjugate deglycosylated glycoproteins in a sample of body fluid from a subject.
492
PATENT ABSTRACTS
A further step involves fluorometric detection of fluoresceinylated carbohydrates incorporated into truncated serum glycoproteins.
5432060 IMMORTALIZED HUMAN BRONCHIAL EPITHELIAL CELL LINE Willey James C; Harris Curtis C Bethesda, MD, UNITED STATES assigned to The United States of America as represented by the Secretary of the Department of Health and Human Services Human bronchial epithelial cell lines permanently transformed by human papilloma viruses have been obtained. These cell lines are useful for the study of growth and differentiation in bronchial carcinoma and the identification of chemical and biological agents that may be useful in the therapy of human lung cancer.
5432061 METHOD AND APPARATUS FOR DETECTING MICROORGANISMS Berndt Klaus W, Schulte Thomas Stewartstown, PA, UNITED STATES assigned to Becton Dickinson and Company The present invention describes a method and apparatus for detecting microorganisms in a large number of blood culture vials using more than one microorganism detection principle for each vial. Two of such possible detection means includes the use of a fluorescent carbon dioxide sensor and scattered photon migration. The apparatus performs a logic linkage of the results of all the detection principles applied. Therefore, an enhanced recovery and an improved accuracy in detecting microorganisms is achieved.
5432064 PROCESS FOR DEPHOSPHORYLATING LINEAR POLYNUCLEOTIDE SUBSTRATE WITH PROSPHATASE FORM ASPERGILLUS NIGER
MarkweUJohn;Versaw Wayne; Osterman John; Kelley Philip Lincoln, NE, UNITED STATES assigned to Board of Regents of the University of Nebraska The present invention relates to the preparation of a novel heat-labile phosphatase enzyme from the filamentous fungus Aspergillus niger. This A. niger phosphatase enzyme has a native molecular weight of approximately 80,000 daltons, and is shown by polyacrylamide gel electrophoresis under denaturing conditions to be an alpha-2 dimer consisting of identical subunits of molecular weight of approximately 37,000 daltons each. The native intact enzyme molecule has an isoelectric point (pI) of 4.6, and exhibits optimal functional activity under reaction conditions of neutral to slightly alkaline pH conditions (about pH 7.0 to about pH 8.5). This enzyme has two characteristics which make it valuable in molecular biology laboratory protocols. First, the enzyme is readily inactivated by mild heating conditions (50 degrees C); and second, the enzyme is highly specific for DNA as a substrate for the hydrolysis reaction; it does not hydrolyze adenosine triphosphate (ATP). This unique characteristic permits the simultaneous dephosphorylation and labeled rephosphorylation of DNA in the presence of polynucleotide kinase and labeled ATP, and eliminates the requirement for a multiplicity of steps in this DNA end-labeling process.
5432065 CYCLE SEQUENCING NON-THERMOSTABLE POLYMERASES
WITH DNA
Fuller Carl W Cleveland Heights, OH, UNITED STATES assigned to United States Biochemical Corporation
5432055 DETECTION OF PORPHYROMONAS GINGIVALIS Evans Mary J; Evans Richard T; G-enco Robert J; Crreenberg Steven; Kuramitsu Howard East Amherst, NY, UNITED STATES The present invention relates to novel compositions comprising P. gingivalis specific oligonucleotides which axe useful as primers to amplify particular regions of the genome of P. gingivalis during enzymatic nucleic acid amplification. The invention also provides a method for the detection of P. gingivalis, which may be present in a clinical specimen, using the P. gingivalis-specific primers and enzymatic nucleic acid amplification. The present invention also relates to P. gingivalis-specific oligonucleotides which are useful as probes to facilitate detection of the amplified regions of P. gingivalis DNA.
5432057 SIMULTANEOUS CALIBRATION HETEROGENEOUS IMMUNOASSAY Litman David J; Ullman Edwin F Mountain View, CA, UNITED STATES assigned to Syva Company An assay method and compositions are provided for determining the presence of an analyte in a sample. The analyte is a member of an immunological pair (mip) comprising ligand and receptor. By providing a first measurement surface capable of specifically binding a labelled reagent in an amount depending upon the presence of analyte in the sample and a second calibration surface capable of binding a second labeled reagent in a manner unaffected by the presence of analyte in the sample, calibration of individual tests can be accomplished simultaneously with the performance of the test itself. A signal producing system includes an enzyme, a catalyst usually bonded to a mip which defines the first labeled reagent for binding to the measurement surface and the same
491
catalyst conjugated to a ligand capable of binding to the calibration surface. Preferably, both labeled reagents have the same composition and the calibration surface includes anti-(first catalyst).
5432058 METHOD
FOR MEASURING
HUMAN CHOLESTEROL ABSORPTION USING METABOLICALLY STABLE ISOTOPES Lange Louis; Ostlund Richard; Bosner Matthew Portola Valley, CA, 94028, UNITED STATES This invention is a method for measuring the ability of a human to absorb cholesterol. The method uses two different cholesterol Iracers, the first injected into the blood stream and the second ingested by the human subject. Atler a waiting period a blood sample is taken from the human subject and analyzed to determine percent cholesterol absorption based on the actual amounts of the two naturally occurring, metabolically stable cholesterol tracers in the blood. The method of this invention is useful in identifying human subjects as high cholesterol absorbers and thereafter administering therapeutic agents to the subject that inhibit the absorption of cholesterol.
5432059 ASSAY FOR GLYCOSYLATION DEFICIENCY DISORDERS Bean Pamela; Terryberry Jeff Los Angeles, CA, UNITED STATES assigned to Specialty Laboratories lnc A method is provided for detecting carbohydrate-deficient glycoproteins in samples taken from subjects with metabolic disorders, such as alcohol abuse and subjects who display a syndrome of carrying abnormal levels of carbohydrate deficient glycoproteins. The method involves steps of reglycosylating with a fluorescent-conjugate deglycosylated glycoproteins in a sample of body fluid from a subject.
492
PATENT ABSTRACTS
A further step involves fluorometric detection of fluoresceinylated carbohydrates incorporated into truncated serum glycoproteins.
5432060 IMMORTALIZED HUMAN BRONCHIAL EPITHELIAL CELL LINE Willey James C; Harris Curtis C Bethesda, MD, UNITED STATES assigned to The United States of America as represented by the Secretary of the Department of Health and Human Services Human bronchial epithelial cell lines permanently transformed by human papilloma viruses have been obtained. These cell lines are useful for the study of growth and differentiation in bronchial carcinoma and the identification of chemical and biological agents that may be useful in the therapy of human lung cancer.
5432061 METHOD AND APPARATUS FOR DETECTING MICROORGANISMS Berndt Klaus W, Schulte Thomas Stewartstown, PA, UNITED STATES assigned to Becton Dickinson and Company The present invention describes a method and apparatus for detecting microorganisms in a large number of blood culture vials using more than one microorganism detection principle for each vial. Two of such possible detection means includes the use of a fluorescent carbon dioxide sensor and scattered photon migration. The apparatus performs a logic linkage of the results of all the detection principles applied. Therefore, an enhanced recovery and an improved accuracy in detecting microorganisms is achieved.
5432064 PROCESS FOR DEPHOSPHORYLATING LINEAR POLYNUCLEOTIDE SUBSTRATE WITH PROSPHATASE FORM ASPERGILLUS NIGER
MarkweUJohn;Versaw Wayne; Osterman John; Kelley Philip Lincoln, NE, UNITED STATES assigned to Board of Regents of the University of Nebraska The present invention relates to the preparation of a novel heat-labile phosphatase enzyme from the filamentous fungus Aspergillus niger. This A. niger phosphatase enzyme has a native molecular weight of approximately 80,000 daltons, and is shown by polyacrylamide gel electrophoresis under denaturing conditions to be an alpha-2 dimer consisting of identical subunits of molecular weight of approximately 37,000 daltons each. The native intact enzyme molecule has an isoelectric point (pI) of 4.6, and exhibits optimal functional activity under reaction conditions of neutral to slightly alkaline pH conditions (about pH 7.0 to about pH 8.5). This enzyme has two characteristics which make it valuable in molecular biology laboratory protocols. First, the enzyme is readily inactivated by mild heating conditions (50 degrees C); and second, the enzyme is highly specific for DNA as a substrate for the hydrolysis reaction; it does not hydrolyze adenosine triphosphate (ATP). This unique characteristic permits the simultaneous dephosphorylation and labeled rephosphorylation of DNA in the presence of polynucleotide kinase and labeled ATP, and eliminates the requirement for a multiplicity of steps in this DNA end-labeling process.
5432065 CYCLE SEQUENCING NON-THERMOSTABLE POLYMERASES
WITH DNA
Fuller Carl W Cleveland Heights, OH, UNITED STATES assigned to United States Biochemical Corporation