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5441867 Pre-activated proteins for labelling oligonucleotide probes
512
PATENT ABSTRACTS
A process for recovering metals from an aqueous metal-ion bearing solution includes the steps of: (a) forming an affinity chromatography matrix by providing a metal bearing protein bound to an insoluble support material to form an immobilized metalloprotein material; (b) introducing into the affinity chromatography matrix a quantity of aqueous metal-containing solution having a pH, redox potential or other property properly adjusted to cause ions of a selected metal entrained within the solution to bind to the immobilized metalloprotein material; (c) washing the matrix with a first buffer which does not elute the metal ions of interest but does remove other species of metal entrained in the solution; and (d) applying a second buffer or solution of appropriate pH, redox potential or other property to the affinity chromatography matrix to elute the metal ions of interest from the immobilized metalloprotein material.
5441867 PRE-ACTIVATED PROTEINS FOR LABELLING OLIGONUCLEOTIDE
PROBES
Garman Andrew; Parker John R Chester, UNITED KINGDOM assigned to Zeneca Limited Pre-activated stable protein reagents are provided. The reagents can be made by reaction of the protein and a heterobifunctional linker and have extended stability especially atter lyophilisation. The reagents can be used in assay kits to form conjugates with proteins eg, antibodies or polynucleotides eg, oligonucleotides probes.
5441872 5441735 METHOD
FOR CONTROLLING
SOFT ROT, BACTERIAL SEEDLING BLIGHT OF RICE AND BLACK ROT Takahara Yoshiyuki; lwabuchi Tetsuya; Shiota Masayuki Saitama, JAPAN assigned to Central Glass Co Ltd A microbial pesticide containing a living Erwinia carotovora subsp, carotovora, particularly, the Erwinia eartovora subsp. carotovora CGE234M403 strain, from which the pathogenicity of sotl rot is deleted by mutagenesis and which is immobilized by mixing with a saecharide such as saccharose, glucose, fructose or sorbitol or beef extract and drying or freeze-drying the mixture under reduced pressure, as an active ingredient is applied to soil or plants, which are liable to suffer from soft rot, bacterial seedling blight of rice and black rot, in the form of a suspension, granules or powder to control the diseases.
METHOD
FOR THE ANALYSIS
OF VITAMIN C Tulley Richard Baton Rouge, LA, UNITED STATES assigned to Board of Supervisors of Louisiana State University and Agricultural and Mechanical College A new enzymatic rate method is disclosed for the analysis of Vitamin C using ascorbate oxidase. Total Vitamin C can be measured in a single analysis, in a procedure amenable to automation. Any dehydroascorbic acid (DHAA) in the sample is reduced to ascorbic acid. Then a coupling agent such as o-phenylenediamine (OPDA) is added to the sample, and any nonspecific reactions with, or spectrometric interferences from, other components of the sample are measured, to be blanked out from the final measurement. Then ascorbate oxidase is added, oxidizing ascorbic acid to DHAA; the DHAA then reacts with the OPDA already present from the prior step, and the quinoxaline derivative product may be measured spectrometrically, eg., by absorbance at 350nm.
Ilnl
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III
Ill
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PATENT ABSTRACTS
A process for recovering metals from an aqueous metal-ion bearing solution includes the steps of: (a) forming an affinity chromatography matrix by providing a metal bearing protein bound to an insoluble support material to form an immobilized metalloprotein material; (b) introducing into the affinity chromatography matrix a quantity of aqueous metal-containing solution having a pH, redox potential or other property properly adjusted to cause ions of a selected metal entrained within the solution to bind to the immobilized metalloprotein material; (c) washing the matrix with a first buffer which does not elute the metal ions of interest but does remove other species of metal entrained in the solution; and (d) applying a second buffer or solution of appropriate pH, redox potential or other property to the affinity chromatography matrix to elute the metal ions of interest from the immobilized metalloprotein material.
5441867 PRE-ACTIVATED PROTEINS FOR LABELLING OLIGONUCLEOTIDE
PROBES
Garman Andrew; Parker John R Chester, UNITED KINGDOM assigned to Zeneca Limited Pre-activated stable protein reagents are provided. The reagents can be made by reaction of the protein and a heterobifunctional linker and have extended stability especially atter lyophilisation. The reagents can be used in assay kits to form conjugates with proteins eg, antibodies or polynucleotides eg, oligonucleotides probes.
5441872 5441735 METHOD
FOR CONTROLLING
SOFT ROT, BACTERIAL SEEDLING BLIGHT OF RICE AND BLACK ROT Takahara Yoshiyuki; lwabuchi Tetsuya; Shiota Masayuki Saitama, JAPAN assigned to Central Glass Co Ltd A microbial pesticide containing a living Erwinia carotovora subsp, carotovora, particularly, the Erwinia eartovora subsp. carotovora CGE234M403 strain, from which the pathogenicity of sotl rot is deleted by mutagenesis and which is immobilized by mixing with a saecharide such as saccharose, glucose, fructose or sorbitol or beef extract and drying or freeze-drying the mixture under reduced pressure, as an active ingredient is applied to soil or plants, which are liable to suffer from soft rot, bacterial seedling blight of rice and black rot, in the form of a suspension, granules or powder to control the diseases.
METHOD
FOR THE ANALYSIS
OF VITAMIN C Tulley Richard Baton Rouge, LA, UNITED STATES assigned to Board of Supervisors of Louisiana State University and Agricultural and Mechanical College A new enzymatic rate method is disclosed for the analysis of Vitamin C using ascorbate oxidase. Total Vitamin C can be measured in a single analysis, in a procedure amenable to automation. Any dehydroascorbic acid (DHAA) in the sample is reduced to ascorbic acid. Then a coupling agent such as o-phenylenediamine (OPDA) is added to the sample, and any nonspecific reactions with, or spectrometric interferences from, other components of the sample are measured, to be blanked out from the final measurement. Then ascorbate oxidase is added, oxidizing ascorbic acid to DHAA; the DHAA then reacts with the OPDA already present from the prior step, and the quinoxaline derivative product may be measured spectrometrically, eg., by absorbance at 350nm.
Ilnl
I
III
Ill
II: