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5445937 Detection of alzheimer's disease and other diseases using an improved photoaffinity labeling method
PATENT ABSTRACTS
5444161
short probes permit detection and discrimination down to the order of a single base.
SUBSTRATES FOR BETA-GALACTOSIDASE Manning Wayne B; Khanna Pyar; Choate Glenda Antioch, CA, UNITED STATES assigned to Microgenics Corporation Galactopyranoside derivative compounds for use as substrates for hydrolysis by enzymes with beta-galactosidase activity are provided. The concentration of the reaction products can be measured by spectroscopy. The compounds find particular use in diagnostic assays for detection of analyte.
5445932 METHOD FOR DETECTION OF A NEW MARKER ASSOCIATED WITH HEPATITIS DELTA VIRUS INFECTION Fields Howard A; Khudyakov Yur; Favorov Michael Marietta, GA, UNITED STATES assigned to The United States of America as represented by the Department of Health and Human Services Reagents and methods for the detection of a marker which is associated with severe forms of hepatitis delta virus infection are described. A vaccine comprising immunogenically active HDAg' polypeptides is also described.
5445933 STRAND DISPLACEMENT ASSAY AND COMPLEX USEFUL THEREFOR Eadie James S; Balgobin Neff G; Buck Harvey Indianapolis, IN, UNITED STATES assigned to Boehringer Mannheim Corporation The invention relates to an improvement in the strand displacement assay. Probes that are at least 20 nucleotide bases in length, but are no more than 40 bases in length, are used. These
519
5445936 METHOD FOR NON-COMPETITIVE BINDING ASSAYS Piran Uri; Riordan William J; Livshin Laurie A Sharon, MA, UNITED STATES assigned to Ciba Coming Diagnostics Corp
A
novel non-competitive immunoassay technique has been developed which not only improves sensitivity, but also is convenient and less susceptible to interfering factors. It is compatible with existing instruments and is an assay that can be run in one test tube. The analyte is reacted with labeled specific binder, after which the mixture is reacted with (1) an insoluble material attached to an analyte derivative and (2) a solid phase carrying a binder. The solid phase is then separated, and the label attached to the solid phase is measured.
5445937 DETECTION OF ALZHEIMER'S DISEASE AND OTHER DISEASES USING AN IMPROVED PHOTOAFFINITY LABELING METHOD Haley Boyd Nicholasville, KY, UNITED STATES assigned to The University of Kentucky Research Foundation An improved method for diagnosing a disease comprising detecting a disease-specific biochemical marker macromolecule within a sample of extracted cerebral spinal fluid or serum is disclosed. In particular, a radioactively labeled photoaffinity probe is used to diagnose a disease. For instance, Alzheimer's disease can be diagnosed by detecting a disease-specific protein having a molecular weight of about 42,000 daltons, i.e., glutamine synthetase. Also included is a method for detecting other disease states, such as cancer, by detecting the presence or absence of specific nucleotide binding proteins.
5444161
short probes permit detection and discrimination down to the order of a single base.
SUBSTRATES FOR BETA-GALACTOSIDASE Manning Wayne B; Khanna Pyar; Choate Glenda Antioch, CA, UNITED STATES assigned to Microgenics Corporation Galactopyranoside derivative compounds for use as substrates for hydrolysis by enzymes with beta-galactosidase activity are provided. The concentration of the reaction products can be measured by spectroscopy. The compounds find particular use in diagnostic assays for detection of analyte.
5445932 METHOD FOR DETECTION OF A NEW MARKER ASSOCIATED WITH HEPATITIS DELTA VIRUS INFECTION Fields Howard A; Khudyakov Yur; Favorov Michael Marietta, GA, UNITED STATES assigned to The United States of America as represented by the Department of Health and Human Services Reagents and methods for the detection of a marker which is associated with severe forms of hepatitis delta virus infection are described. A vaccine comprising immunogenically active HDAg' polypeptides is also described.
5445933 STRAND DISPLACEMENT ASSAY AND COMPLEX USEFUL THEREFOR Eadie James S; Balgobin Neff G; Buck Harvey Indianapolis, IN, UNITED STATES assigned to Boehringer Mannheim Corporation The invention relates to an improvement in the strand displacement assay. Probes that are at least 20 nucleotide bases in length, but are no more than 40 bases in length, are used. These
519
5445936 METHOD FOR NON-COMPETITIVE BINDING ASSAYS Piran Uri; Riordan William J; Livshin Laurie A Sharon, MA, UNITED STATES assigned to Ciba Coming Diagnostics Corp
A
novel non-competitive immunoassay technique has been developed which not only improves sensitivity, but also is convenient and less susceptible to interfering factors. It is compatible with existing instruments and is an assay that can be run in one test tube. The analyte is reacted with labeled specific binder, after which the mixture is reacted with (1) an insoluble material attached to an analyte derivative and (2) a solid phase carrying a binder. The solid phase is then separated, and the label attached to the solid phase is measured.
5445937 DETECTION OF ALZHEIMER'S DISEASE AND OTHER DISEASES USING AN IMPROVED PHOTOAFFINITY LABELING METHOD Haley Boyd Nicholasville, KY, UNITED STATES assigned to The University of Kentucky Research Foundation An improved method for diagnosing a disease comprising detecting a disease-specific biochemical marker macromolecule within a sample of extracted cerebral spinal fluid or serum is disclosed. In particular, a radioactively labeled photoaffinity probe is used to diagnose a disease. For instance, Alzheimer's disease can be diagnosed by detecting a disease-specific protein having a molecular weight of about 42,000 daltons, i.e., glutamine synthetase. Also included is a method for detecting other disease states, such as cancer, by detecting the presence or absence of specific nucleotide binding proteins.