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5451506 Oncostatin M and novel compositions having anti-neoplastic activity
480
PATENT ABSTRACTS
control and the automated processing thereof. The carriers are processed in stepwise treatments that expose, amplify, and detect the presence or absence of specific genetic entities in each sample. The nucleic acids or other desired biological components held within the matrices are treated by one or more of the techniques such as amplification, electrophoresis, analyte-receptor binding or hybridization as selected for the desired analysis. Different types of carriers are used for different kinds of diagnostic tests or test panels. Different primer and polymerase molecules may be used to replicate the target sequences of nucleic acids in the sample. The system also includes a heating and a passive cooling system that will provide rapid thermal cycling in a two-dimensional format for denaturation and amplification protocols so requiring them, or maintain constant temperatures for isothermal amplification and hybridization protocols. The system is adaptable to a variety of non-isotopic labels. The two-dimensional format lends itself to digitizing signals in a two-dimensional array for image analysis. Microscopic interpretation is possible with or without image analysis.
5451502 RESTRICTION AMPLIFICATION
The present invention relates to a method, reagent and kit for the determination of the presence of target nucleotide sequences by restriction amplification. In the process to detect nucleic acid sequences a target molecule containing a specific restriction site is hybridized with a labeled probe containing a sequence homologous to at least 28 bases of the target molecule. The probe is cleaved with a restriction enzyme that releases the probe for detection if the probe hybridizes to the specific target. Thus, the cleaved probe constantly regenerates and is highly detectable if the target sequence is present in the assay.
m
ONCOSTATIN
M AND NOVEL
COMPOSITIONS ANTI-NEOPLASTIC
HAVING ACTIVITY
Shoyab Mohammed; Zarling Joyce M; Marquardt Han; Hanson Marcia B; Linsley Peter S Seattle, WA, UNITED STATES assigned to Oncogen Limited Partnership Novel compositions comprising Oncostatin M and congeners thereof, as well as methods for their preparation and methods for their use are provided. The compositions may be prepared by isolation from natural sources, or by recombinant means in either prokaryotic or eukaryotic host cells. In addition, the DNA and polypeptide sequences for Oncostatin M are disclosed. The compositions find use in modulating growth of cells, in particular inhibition of tumor cell proliferation, and stimulation of normal cell growth, especially ceils involved in hematopoiesis. Cell growth inhibition compositions may additionally include an adjunctive agent comprising at least one of a transforming growth factor, tumor necrosis factor, or an interferon. Receptors having high affinity for Oncostatin M may additionally be used to screen polypeptides for Oncostatin M-like activity. Methods for use of antibodies to the compositions and probes specific for Oncostatin M mRNA as a means for detecting tumor cells are also provided.
ASSAY
George Albert L Gaithersburg, MD, UNITED STATES assigned to Oncor Inc
_
5451506
5451512 METHODS AND REAGENTS FOR HLA CLASS I A LOCUS DNA TYPING Apple Raymond; Bugawan Teodorica L; Erlich Henry A San Francisco, CA, UNITED STATES assigned to Hoffmann-La Roche Inc Primers for amplification of specific nucleic acid sequences of the second and third exon of HLA Class I A gene and probes for identifying polymorphic sequences contained in the amplified DNA can be used in processes for typing homozygous or heterozygous samples
PATENT ABSTRACTS
control and the automated processing thereof. The carriers are processed in stepwise treatments that expose, amplify, and detect the presence or absence of specific genetic entities in each sample. The nucleic acids or other desired biological components held within the matrices are treated by one or more of the techniques such as amplification, electrophoresis, analyte-receptor binding or hybridization as selected for the desired analysis. Different types of carriers are used for different kinds of diagnostic tests or test panels. Different primer and polymerase molecules may be used to replicate the target sequences of nucleic acids in the sample. The system also includes a heating and a passive cooling system that will provide rapid thermal cycling in a two-dimensional format for denaturation and amplification protocols so requiring them, or maintain constant temperatures for isothermal amplification and hybridization protocols. The system is adaptable to a variety of non-isotopic labels. The two-dimensional format lends itself to digitizing signals in a two-dimensional array for image analysis. Microscopic interpretation is possible with or without image analysis.
5451502 RESTRICTION AMPLIFICATION
The present invention relates to a method, reagent and kit for the determination of the presence of target nucleotide sequences by restriction amplification. In the process to detect nucleic acid sequences a target molecule containing a specific restriction site is hybridized with a labeled probe containing a sequence homologous to at least 28 bases of the target molecule. The probe is cleaved with a restriction enzyme that releases the probe for detection if the probe hybridizes to the specific target. Thus, the cleaved probe constantly regenerates and is highly detectable if the target sequence is present in the assay.
m
ONCOSTATIN
M AND NOVEL
COMPOSITIONS ANTI-NEOPLASTIC
HAVING ACTIVITY
Shoyab Mohammed; Zarling Joyce M; Marquardt Han; Hanson Marcia B; Linsley Peter S Seattle, WA, UNITED STATES assigned to Oncogen Limited Partnership Novel compositions comprising Oncostatin M and congeners thereof, as well as methods for their preparation and methods for their use are provided. The compositions may be prepared by isolation from natural sources, or by recombinant means in either prokaryotic or eukaryotic host cells. In addition, the DNA and polypeptide sequences for Oncostatin M are disclosed. The compositions find use in modulating growth of cells, in particular inhibition of tumor cell proliferation, and stimulation of normal cell growth, especially ceils involved in hematopoiesis. Cell growth inhibition compositions may additionally include an adjunctive agent comprising at least one of a transforming growth factor, tumor necrosis factor, or an interferon. Receptors having high affinity for Oncostatin M may additionally be used to screen polypeptides for Oncostatin M-like activity. Methods for use of antibodies to the compositions and probes specific for Oncostatin M mRNA as a means for detecting tumor cells are also provided.
ASSAY
George Albert L Gaithersburg, MD, UNITED STATES assigned to Oncor Inc
_
5451506
5451512 METHODS AND REAGENTS FOR HLA CLASS I A LOCUS DNA TYPING Apple Raymond; Bugawan Teodorica L; Erlich Henry A San Francisco, CA, UNITED STATES assigned to Hoffmann-La Roche Inc Primers for amplification of specific nucleic acid sequences of the second and third exon of HLA Class I A gene and probes for identifying polymorphic sequences contained in the amplified DNA can be used in processes for typing homozygous or heterozygous samples