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5459031 Methods for controlling sialic acid derivatives in recombinant glycoproteins
PATENT ABSTRACTS
bacteria generate metabolic energy from the oxidation of organic compounds, but use oxidized forms of sulfur as an electron acceptor. Because the biocatalyst is present in the form of bacteria in an aqueous suspension, whereas the reacting substr~te consists of hydrocarbon molecules in an organic phase, the actual desulfurization reaction takes place at the aqueous-organic interphase. To ensure adequate interfacial contacting and mass transfer, a biphasic electrostatic bioreactor system is utilized. The bioreactor is utilized to disperse and recoalesce a biocatalyst contained in the aqueous liquid phase into the organic liquid phase containing the sulfur. High-intensity electrical fields rupture the aqueous drops into a plurality ofmicrodroplets and induce continuous coalescence and redispersion as the microdroplets travel through the organic phase, thus increasing surface area. As the aqueous microdroplets progress through the organic phase, the biocatalyst then reacts with the sulfur to produce hydrogen sulfide which is then removed from the bioreactor. The organic liquid, now free of the sulfur, is ready for immediate use or further processing.
5458761 METHOD FOR ANALYZING NUCLEIC ACID OR PROTEIN AND APPARATUS THEREFOR Kamahori Masao; Fujita Takeshi; Umemura Shinichir; Yamada Takashi Hatoyama, JAPAN assigned to Hitachi Ltd Analysis of a nucleic acid samples having thousands of bases is conducted by capillary electrophoresis. The electrophoretic section is provided with a first capillary filled with an agarose gel and a second capillary filled with a polyacrylamide gel. An on-column detector is incorporated with the second capillary for optical detection. To fill a capillary with a gel, a solution is fed under high pressure from a first flow channel through a switching valve into a second flow channel connected to the capillary. To inject a sample in the capillary, a sample injector is connected to a switching valve passage, and a buffer solution is connected to the capillary through a flow channel and the switching valve. After switching the valve, the
543
first passage is incorporated into the flow channel between the buffer solution and the capillary being filled. Then, the sample is electro-kinetically injected into the capillary. When conducting genetic polymorphism by electrophoresis, temperature control elements are provided to maintain the capillary at a predetermined temperature and a DNA sample device is provided to heat the sample to a temperature higher than a disassociation temperature thereof for directly injecting the heated sample into the capillary.
5459015 HIGH-AFFINITY RNA LIGANDS OF BASIC FIBROBLAST GROWTH FACTOR Janjic Nebojsa; Gold Larry Boulder, CO, UNITED STATES assigned to NeXstar Pharmaceuticals Inc Methods are described for the identification and preparation of nucleic acid ligands and ligand solutions to basic fibroblast growth factor (bFGF). Included in the invention are nucleic acid ligands to bFGF which are inhibitors of bFGF and T-amino-modified RNA ligands to bFGF.
5459031 METHODS FOR CONTROLLING SIALIC ACID DERIVATIVES IN RECOMBINANT GLYCOPROTEINS Blumen Tracy K; G-rampp Gustavo E; Hettwer David J Thousand Oaks, CA, UNITED STATES assigned to Amgen Inc The invention relates to the effects of certain cell culture and/or production parameters on the carbohydrate composition of a cell culture-derived recombinant glycoprotein. The methods of the invention demonstrate a practical method for controlling levels of a sialic acid derivative, NGNA, in secreted recombinant glycoproteins which will be useful in a variety of bioprocessing systems.
bacteria generate metabolic energy from the oxidation of organic compounds, but use oxidized forms of sulfur as an electron acceptor. Because the biocatalyst is present in the form of bacteria in an aqueous suspension, whereas the reacting substr~te consists of hydrocarbon molecules in an organic phase, the actual desulfurization reaction takes place at the aqueous-organic interphase. To ensure adequate interfacial contacting and mass transfer, a biphasic electrostatic bioreactor system is utilized. The bioreactor is utilized to disperse and recoalesce a biocatalyst contained in the aqueous liquid phase into the organic liquid phase containing the sulfur. High-intensity electrical fields rupture the aqueous drops into a plurality ofmicrodroplets and induce continuous coalescence and redispersion as the microdroplets travel through the organic phase, thus increasing surface area. As the aqueous microdroplets progress through the organic phase, the biocatalyst then reacts with the sulfur to produce hydrogen sulfide which is then removed from the bioreactor. The organic liquid, now free of the sulfur, is ready for immediate use or further processing.
5458761 METHOD FOR ANALYZING NUCLEIC ACID OR PROTEIN AND APPARATUS THEREFOR Kamahori Masao; Fujita Takeshi; Umemura Shinichir; Yamada Takashi Hatoyama, JAPAN assigned to Hitachi Ltd Analysis of a nucleic acid samples having thousands of bases is conducted by capillary electrophoresis. The electrophoretic section is provided with a first capillary filled with an agarose gel and a second capillary filled with a polyacrylamide gel. An on-column detector is incorporated with the second capillary for optical detection. To fill a capillary with a gel, a solution is fed under high pressure from a first flow channel through a switching valve into a second flow channel connected to the capillary. To inject a sample in the capillary, a sample injector is connected to a switching valve passage, and a buffer solution is connected to the capillary through a flow channel and the switching valve. After switching the valve, the
543
first passage is incorporated into the flow channel between the buffer solution and the capillary being filled. Then, the sample is electro-kinetically injected into the capillary. When conducting genetic polymorphism by electrophoresis, temperature control elements are provided to maintain the capillary at a predetermined temperature and a DNA sample device is provided to heat the sample to a temperature higher than a disassociation temperature thereof for directly injecting the heated sample into the capillary.
5459015 HIGH-AFFINITY RNA LIGANDS OF BASIC FIBROBLAST GROWTH FACTOR Janjic Nebojsa; Gold Larry Boulder, CO, UNITED STATES assigned to NeXstar Pharmaceuticals Inc Methods are described for the identification and preparation of nucleic acid ligands and ligand solutions to basic fibroblast growth factor (bFGF). Included in the invention are nucleic acid ligands to bFGF which are inhibitors of bFGF and T-amino-modified RNA ligands to bFGF.
5459031 METHODS FOR CONTROLLING SIALIC ACID DERIVATIVES IN RECOMBINANT GLYCOPROTEINS Blumen Tracy K; G-rampp Gustavo E; Hettwer David J Thousand Oaks, CA, UNITED STATES assigned to Amgen Inc The invention relates to the effects of certain cell culture and/or production parameters on the carbohydrate composition of a cell culture-derived recombinant glycoprotein. The methods of the invention demonstrate a practical method for controlling levels of a sialic acid derivative, NGNA, in secreted recombinant glycoproteins which will be useful in a variety of bioprocessing systems.