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5468614 System to detect protein-protein interactions
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PATENT ABSTRACTS
5468612
5468614
9804GENE AND METHODS USE THEREOF Cohen Edward H; Landgraf Somerville, MA, UNITED assigned to Cytomed Inc
OF
Bryan E STATES
A substantially pure preparation of a 9804 DNA encoding a hematopoietic stem cell protein; antibodies to the 9804 gene product; methods of purifying 9804-expressing cells; and methods of screening for 9804 homologs are disclosed.
5468613 PROCESS FOR DETECTING SPECIFIC NUCLEOTIDE VARIATIONS AND GENETIC POLYMORPHISMS PRESENT IN NUCLEIC ACIDS Erlich Henry; Horn Glenn; Saiki Randall K; Mullis Kary CA, UNITED STATES assigned to Hoffmann-La Roche Inc Single or multiple nucleotide variations in nucleic acid sequence can be detected in nucleic acids by a process whereby the sample suspected of containing the relevant nucleic acid is repeatedly treated with primers, nucleotide triphosphates, and an agent for polymerization of the triphosphates and then denatured, in a process which amplifies the sequence containing the nucleotide variation if it is present. In one embodiment, the sample is spotted on a membrane and treated with a labeled sequence-specific oligonucleotide probe. Hybridization of the probe to the sample is detected by the label on the probe.
SYSTEM TO DETECT PROTEIN-PROTEIN INTERACTIONS Fields Startle; Song Ok-Kyu East Setauket, NY, UNITED STATES assigned to The Research Foundation of State University of New York A method and kit are provided for detecting the interaction between a first test protein and a second test protein, in vivo, using reconstitution of the activity of a transcriptional activator. This reconstitution makes use of chimeric genes which express hybrid proteins. Two types of hybrid proteins are prepared. The first hybrid contains the DNA-binding domain of a transcriptional activator fused to the first test protein. The second hybrid protein contains a transcriptional activation domain fused to the second test protein. If the two test proteins are able to interact, they bring into close proximity the two domains of the transcriptional activator. This proximity is sufficient to cause transcription, which can be detected by the activity of a marker gene which contains a binding site for the DNA-binding domain.
5468616 METHOD AND APPARATUS FOR RAPID MIXING OF SMALL VOLUMES FOR ENHANCING BIOLOGICAL REACTIONS Coulter Wallace H; Hollinger John D; Kortright Kenneth; Russell Thomas; Rodriguez Carlo; Paul Ronald Miami Springs, FL, UNITED STATES assigned to Coulter Corporation A method and apparatus for accelerating at least one definitive biological reaction
PATENT ABSTRACTS
5468612
5468614
9804GENE AND METHODS USE THEREOF Cohen Edward H; Landgraf Somerville, MA, UNITED assigned to Cytomed Inc
OF
Bryan E STATES
A substantially pure preparation of a 9804 DNA encoding a hematopoietic stem cell protein; antibodies to the 9804 gene product; methods of purifying 9804-expressing cells; and methods of screening for 9804 homologs are disclosed.
5468613 PROCESS FOR DETECTING SPECIFIC NUCLEOTIDE VARIATIONS AND GENETIC POLYMORPHISMS PRESENT IN NUCLEIC ACIDS Erlich Henry; Horn Glenn; Saiki Randall K; Mullis Kary CA, UNITED STATES assigned to Hoffmann-La Roche Inc Single or multiple nucleotide variations in nucleic acid sequence can be detected in nucleic acids by a process whereby the sample suspected of containing the relevant nucleic acid is repeatedly treated with primers, nucleotide triphosphates, and an agent for polymerization of the triphosphates and then denatured, in a process which amplifies the sequence containing the nucleotide variation if it is present. In one embodiment, the sample is spotted on a membrane and treated with a labeled sequence-specific oligonucleotide probe. Hybridization of the probe to the sample is detected by the label on the probe.
SYSTEM TO DETECT PROTEIN-PROTEIN INTERACTIONS Fields Startle; Song Ok-Kyu East Setauket, NY, UNITED STATES assigned to The Research Foundation of State University of New York A method and kit are provided for detecting the interaction between a first test protein and a second test protein, in vivo, using reconstitution of the activity of a transcriptional activator. This reconstitution makes use of chimeric genes which express hybrid proteins. Two types of hybrid proteins are prepared. The first hybrid contains the DNA-binding domain of a transcriptional activator fused to the first test protein. The second hybrid protein contains a transcriptional activation domain fused to the second test protein. If the two test proteins are able to interact, they bring into close proximity the two domains of the transcriptional activator. This proximity is sufficient to cause transcription, which can be detected by the activity of a marker gene which contains a binding site for the DNA-binding domain.
5468616 METHOD AND APPARATUS FOR RAPID MIXING OF SMALL VOLUMES FOR ENHANCING BIOLOGICAL REACTIONS Coulter Wallace H; Hollinger John D; Kortright Kenneth; Russell Thomas; Rodriguez Carlo; Paul Ronald Miami Springs, FL, UNITED STATES assigned to Coulter Corporation A method and apparatus for accelerating at least one definitive biological reaction