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5470728 Formation of and dehydration of hydroxylated diphenyl acetylenes using pseudomonas ATCC 55272
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PATENTABSTRACTS
site-specific recombination site such as lambda att P. The host cell chromosome contains a second site-specific recombination site. When the chromosomal transfer DNA is introduced into the host cell, expression of an integration enzyme such as integrase causes the integration of the chromosomal transfer DNA into the host cell chromosome at the second recombination site.
A restriction enzyme is obtained by cultivating a strain of Brevibacterium linens and recovering the restriction enzyme. The restriction enzyme, which has the same specificity as AvrII, cleaves the following nucleotide sequence specifically at the arrow-marked sites: (*See Patent for Chemical Structure*).
5470728 5470733 FORMATION
OF AND
DEHYDRATION OF HYDROXYLATED DIPHENYL ACETYLENES PSEUDOMONAS
USING ATCC 55272
Grund Alan Manitowoc, STATES assigned to Resources
WI, UNITED Bio-Technical
Cis-dihydrodiol compounds of the formula (*See Patent for Chemical Structure*) where n is 0 or 1 (preferably 0) are produced by the microbial oxidation of a diphenylaceylene compound of the formula (*See Patent for Chemical Structure*) using a mutant of a Pseudomonas bacteria at 25 degrees to 35 degrees C. and pH 6-8. The cis-dihydrodiol compound is in turn treated with an aqueous solution of a base to produce a corresponding 3-hydroxydiphenylacetylene compound or with an aqueous solution of an acid to produce a corresponding 2-hydroxyacetylene compound. The mutant strain is preferably Pseudomonas ATCC 55272.
5470732 RESTRICTION ENZYME FROM BREVIBACTERIUM LINENS Yamamoto Katsuhik; Sagawa Hiroaki; Kotani Hirokazu; Hiraoka Nobutsugu; Nakamura Teruya Otsu, JAPAN assigned to Takara Shuzo Co Ltd
CALCIUM
FREE SUBTILISIN MUTANTS
Bryan Philip N; Alexander Patrick A; Strausberg Susan L Silver Spring, MD, UNITED STATES assigned to University of Maryland Novel calcium free subtilisin mutants are taught, in particular a subtilisin which has been mutated to eliminate amino acids 75-83.
5470734 RECOMBINANT
HERPESVIRUS
OF TURKEYS AND LIVE VECTOR VACCINES DERIVED THEREOF Sondermeijer Paulus J A; Claessens Johannes A J; Mockett Albert P A Boxmeer, NETHERLANDS assigned to Akzo Nobel NV The present invention is concerned with a recombinant Herpesvirus of Turkeys (HVT), containing a heterologous gene incorporated into an insertion region of the genome of HVT. The invention also relates to a vector vaccine comprising such a recombinant HVT which expresses a heterologous antigenic polypeptide and induces an adequate immune response on infection of an appropriate host animal.
PATENTABSTRACTS
site-specific recombination site such as lambda att P. The host cell chromosome contains a second site-specific recombination site. When the chromosomal transfer DNA is introduced into the host cell, expression of an integration enzyme such as integrase causes the integration of the chromosomal transfer DNA into the host cell chromosome at the second recombination site.
A restriction enzyme is obtained by cultivating a strain of Brevibacterium linens and recovering the restriction enzyme. The restriction enzyme, which has the same specificity as AvrII, cleaves the following nucleotide sequence specifically at the arrow-marked sites: (*See Patent for Chemical Structure*).
5470728 5470733 FORMATION
OF AND
DEHYDRATION OF HYDROXYLATED DIPHENYL ACETYLENES PSEUDOMONAS
USING ATCC 55272
Grund Alan Manitowoc, STATES assigned to Resources
WI, UNITED Bio-Technical
Cis-dihydrodiol compounds of the formula (*See Patent for Chemical Structure*) where n is 0 or 1 (preferably 0) are produced by the microbial oxidation of a diphenylaceylene compound of the formula (*See Patent for Chemical Structure*) using a mutant of a Pseudomonas bacteria at 25 degrees to 35 degrees C. and pH 6-8. The cis-dihydrodiol compound is in turn treated with an aqueous solution of a base to produce a corresponding 3-hydroxydiphenylacetylene compound or with an aqueous solution of an acid to produce a corresponding 2-hydroxyacetylene compound. The mutant strain is preferably Pseudomonas ATCC 55272.
5470732 RESTRICTION ENZYME FROM BREVIBACTERIUM LINENS Yamamoto Katsuhik; Sagawa Hiroaki; Kotani Hirokazu; Hiraoka Nobutsugu; Nakamura Teruya Otsu, JAPAN assigned to Takara Shuzo Co Ltd
CALCIUM
FREE SUBTILISIN MUTANTS
Bryan Philip N; Alexander Patrick A; Strausberg Susan L Silver Spring, MD, UNITED STATES assigned to University of Maryland Novel calcium free subtilisin mutants are taught, in particular a subtilisin which has been mutated to eliminate amino acids 75-83.
5470734 RECOMBINANT
HERPESVIRUS
OF TURKEYS AND LIVE VECTOR VACCINES DERIVED THEREOF Sondermeijer Paulus J A; Claessens Johannes A J; Mockett Albert P A Boxmeer, NETHERLANDS assigned to Akzo Nobel NV The present invention is concerned with a recombinant Herpesvirus of Turkeys (HVT), containing a heterologous gene incorporated into an insertion region of the genome of HVT. The invention also relates to a vector vaccine comprising such a recombinant HVT which expresses a heterologous antigenic polypeptide and induces an adequate immune response on infection of an appropriate host animal.