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5486470 Purified Herpes Simplex Virus protease and methods of purification
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PATEST ABSTRACTS
including streptavidin/avidin, glyco-proteins, lectins, hormones,antigens, drugs, antibodies and antigen binding fragments thereof, or any other selectively bindable reagent by chemical crosslinking means. The present invention also encompasses conjugates produced by this method, and methods of use of such conjugates.
5486458 METHOD OF QUANTITATIVE ASSAY FOR 1,5-ANHYDROGLUCITOL Kojima Ryo; Sato Yoshir; Nagasawa Takeshi Koriyama, JAPAN assigned to Nitto Boseki Co Ltd There is provided a method of quantitative assay for 1,5-anhydroglucitol in a specimen characterized by effectively removing maltose present in the specimen using alpha-glucosidase. Maltose present in a specimen is previously converted into glucose by the action of alpha-glucosidase derived from a microorganism belonging to the genus Bacillus to remove the affect by maltose. Then 1,5anhydroglucitol in the specimen is quantitatively determined.
5486459 BIOLOGICALLY RELEVANT METHODS FOR THE RAPID DETERMINATION OF STERILITY Burnham Jeffrey C; Hageage George J; Jambard-Sweet Douglas; Hendricks Judy Maumee, OH, UNITED STATES assigned to Medical College of Ohio The invention is directed to biological processes and apparatus for determining
the efficacy of a sterilization cycle based upon the recovery of activity of interactive enzyme systems comprising enzymes, coenzymes, catalysts, cofactors, substrates or any other necessary reagents. The invention provides a vital process for expediting sterility verification before utilization of the articles thought to be sterilized. The invention involves the rapid detection of any surviving interactive enzymatic activity which directly relates to the probability of any biological spores surviving in a test sample. An absence of a change indicates that the sterilization process had inactivated the enzyme system thereby preventing the interactive reaction from taking place which is a rapid equivalent to directly detecting the survivability of bacterial spores in a similar test. The methods and apparatus of the invention are useful in the health care industry such as in hospitals, laboratories, and research institutions, in food and environmental technology, and in all technologies which utilize sterilization in manufacturing, production or waste disposal.
5486470 PURIFIED HERPES SIMPLEX VIRUS PROTEASE AND METHODS OF PURIFICATION Darke Paul L; Hall Dawn L; Kuo Blue Bell, PA, UNITED Lawrence C STATES assigned to Merck & Co Inc A method of purifying proteases from the Herpes Simplex Virus 1 and 2 is taught. A precursor protease is cloned in a host cell and then separated from the cell culture using cation exchange chromatography. The resulting protease is subjected to autoprocessing conditions and an autolytic in mature cleavage occurs, resulting protease.
PATEST ABSTRACTS
including streptavidin/avidin, glyco-proteins, lectins, hormones,antigens, drugs, antibodies and antigen binding fragments thereof, or any other selectively bindable reagent by chemical crosslinking means. The present invention also encompasses conjugates produced by this method, and methods of use of such conjugates.
5486458 METHOD OF QUANTITATIVE ASSAY FOR 1,5-ANHYDROGLUCITOL Kojima Ryo; Sato Yoshir; Nagasawa Takeshi Koriyama, JAPAN assigned to Nitto Boseki Co Ltd There is provided a method of quantitative assay for 1,5-anhydroglucitol in a specimen characterized by effectively removing maltose present in the specimen using alpha-glucosidase. Maltose present in a specimen is previously converted into glucose by the action of alpha-glucosidase derived from a microorganism belonging to the genus Bacillus to remove the affect by maltose. Then 1,5anhydroglucitol in the specimen is quantitatively determined.
5486459 BIOLOGICALLY RELEVANT METHODS FOR THE RAPID DETERMINATION OF STERILITY Burnham Jeffrey C; Hageage George J; Jambard-Sweet Douglas; Hendricks Judy Maumee, OH, UNITED STATES assigned to Medical College of Ohio The invention is directed to biological processes and apparatus for determining
the efficacy of a sterilization cycle based upon the recovery of activity of interactive enzyme systems comprising enzymes, coenzymes, catalysts, cofactors, substrates or any other necessary reagents. The invention provides a vital process for expediting sterility verification before utilization of the articles thought to be sterilized. The invention involves the rapid detection of any surviving interactive enzymatic activity which directly relates to the probability of any biological spores surviving in a test sample. An absence of a change indicates that the sterilization process had inactivated the enzyme system thereby preventing the interactive reaction from taking place which is a rapid equivalent to directly detecting the survivability of bacterial spores in a similar test. The methods and apparatus of the invention are useful in the health care industry such as in hospitals, laboratories, and research institutions, in food and environmental technology, and in all technologies which utilize sterilization in manufacturing, production or waste disposal.
5486470 PURIFIED HERPES SIMPLEX VIRUS PROTEASE AND METHODS OF PURIFICATION Darke Paul L; Hall Dawn L; Kuo Blue Bell, PA, UNITED Lawrence C STATES assigned to Merck & Co Inc A method of purifying proteases from the Herpes Simplex Virus 1 and 2 is taught. A precursor protease is cloned in a host cell and then separated from the cell culture using cation exchange chromatography. The resulting protease is subjected to autoprocessing conditions and an autolytic in mature cleavage occurs, resulting protease.