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5487990 Glucose-regulated promoter of yeast acetyl-CoA hydrolase
PATENT ABSTRACTS
DNA CLONE ENCODING AN ETHYLENE-FORMING ENZYME, CONSTRUCTS, PLANT CELLS AND PLANTS BASED THEREON Bird Cohn R; Ray John; Schuch Wolfgang Bra&tell, UNITED KINGDOM assigned to Zeneca Limited PCT No. PCT/GB91/02272 Sec. 371 Date Aug. 2, 1993 Sec. 102(e) Date Aug. 2, 1993 PCT Filed Dec. 19, 1991 PCT Pub. No. WO92/11371 PCT Pub. Date Jul. 9, 1992. DNA clones, e.g. M13-1, comprising at least part of a gene derived from melon that encodes ethylene-forming enzyme. Such clones may comprise DNA constructs including plant promoters capable of expressing RNA in plant cells. Such constructs may be used to inhibit production of ethylene-forming enzyme in transformed plants, and thereby to produce slower-ripening fruit, particularly melons. The clones may be obtained by PCR using specific oligo-nucleotide primers.
5486467 CATALASE FROM BACILLUS SUBTILIS IAM 1026 (FERM BP-4844) Fusho Yuichi; Yajima Kanagawa, JAPAN assigned Denko Kabushiki Kaisha
Yoshihiro to Showa
A microbial catalase having a catalase activity at 0 degrees C. of 95% or more of its catalase activity at 30 degrees C., when measured at pH 7,and a process for producing the same. The microbial catalase preferably has (1) an operative temperature of 0 degrees to 60 degrees C. and an optimum temperature of 0 degrees to 30
207
degrees C., (2) an optimum pH of 7 to 10, (3) a resistance to 10 mM potassium fluoride, (4) a molecular weight is 65,000+/-3,000, when measured by SDS polyacrylamide gel electrophoresis, and (5) an isoelectric point is about 4.8, when measured by isoelectric focusing. The catalase is obtainable from Bacillus subtilis IAM 1206 (FERM BP-4844) or a mutant strain thereof.
5487886 AMINO ACID BETA-LYASE ENZYME INHIBITORS AS DEODORANTS Lyon Sue B; O’Neal Cliffor; Van Der Lee Hermes; Rogers Brian Bethesda, MD, UNITED STATES assigned to The Gillette Company A deodorant composition comprising a body odor suppressing effective amount of an inhibitor of an amino acid B-lyase enzyme which catalyazes the formation of human body malodor, wherein the inhibitor is a compound of the formula NH2-C(CH3) (COOH)-CH2SR where R is a hydrogen, phenyl; C 1-C8 alkyl which is unsubstituted or substituted by a phenyl a hydroxy group, a benzyloxy or benzyloxycarbonyl group, a halogen or an amine group, in a dermatologically acceptable vehicle.
5487990 GLUCOSE-REGULATED PROMOTER OF YEAST ACETYL-COA HYDROLASE Smith John A; Lee Fang-Jen S; Lin Lee-We Scotch Plains, NJ, UNITED STATES assigned to The General Hospital Corporation
208
PATENT ABSTRACTS
This invention is directed to the acetyl-CoA hydrolase enzyme of yeast and to a method for purifying ace@-CoA hydrolase. The enzyme has a molecular weight of about 64,000 daltons and an enzyme activity of greater than 20 units wherein one unit of acetyl CoA hydrolase activity is defined as the amount of enzyme able to inhibit 1 unit of acetyltransferase activity and 1 unit of acetyltransferase activity is defined as the amount of enzyme needed to transfer 1 pmol of (3H) acetyl group from (3H) acetyl coenzyme A to ACTH (l-24) in the acetyltransferase standard enzyme assay under standard conditions. The invention is further directed to the glucose-repressible promoter which regulates the expression of the acetyl-CoA hydrolase gene in yeast, to vectors incorporating this promoter and to host cells transformed with such vectors.
5488062 2SACCHARINYLMETHYL HETEROCYCLIC CARBOXYLATES PROTEOLYTIC INHIBITORS
USEFUL ENZYME
AS
AND
COMPOSITIONS AND METHOD OF USE THEREOF Dunlap Richard; Hlasta Dennis; Desai Ranjit; Latimer Lee; Subramanyam Chakrapani; Court John J; Bell Malcolm R; Kumar Virendra Penfield, NY, UNITED STATES assigned to Sterling Winthrop Inc 4-R4-RS-2-Saccharinylmethyl heterocyclic carboxylates, useful in the treatment of degenerative diseases, are prepared by reacting a 4-R4-R5-2-halomethylsaccharin with either a heterocyclic carboxylic acid in the presence of an acid-acceptor or the alkali metal salt of a heterocyclic carboxylic acid.
5492813 MUTEINS
OF
BETA-GALACTOSIDASE FRAGMENTS HAVING INCREASED
ACTIVITY
Eisenbeis Scott J; Boguslawski Sophie J; Krevolin Mark; Ledden David J Indianapolis, IN, UNITED STATES assigned to Boehringer Mannheim Corporation Muteins of enzyme acceptor polypeptide fragments of beta-galactosidase are provided which exhibit substantially increased kinetic complementation activity with no significant loss in stability. A preferred enzyme acceptor fragment has an amino acid other than cysteine located at position 500 of the natural sequence. An especially preferred substitution is serine or valine. Other preferred muteins have an amino acid other than methionine located at position 443, with leucine being especially preferred, or an amino acid other than cysteine at position 76, with leucine being an especially preferred substitution. Also provided are methods for producing the novel muteins, reagent compositions comprising the novel muteins, and immunoassay methods for determining an analyte in which the novel mutein recombines with an enzyme donor polypeptide fragment to form enzymatically active beta-galactosidase. 5492822 METHOD FOR RECOVERING 100,000 DALTON PURIFIED MOLECULAR WEIGHT FRACTION OF HUMAN PANCREATIC CHOLESTEROL ESTERASE Lange Louis G; Spilburg Curtis A; St Louis, MO, Kinnunen Paula M
DNA CLONE ENCODING AN ETHYLENE-FORMING ENZYME, CONSTRUCTS, PLANT CELLS AND PLANTS BASED THEREON Bird Cohn R; Ray John; Schuch Wolfgang Bra&tell, UNITED KINGDOM assigned to Zeneca Limited PCT No. PCT/GB91/02272 Sec. 371 Date Aug. 2, 1993 Sec. 102(e) Date Aug. 2, 1993 PCT Filed Dec. 19, 1991 PCT Pub. No. WO92/11371 PCT Pub. Date Jul. 9, 1992. DNA clones, e.g. M13-1, comprising at least part of a gene derived from melon that encodes ethylene-forming enzyme. Such clones may comprise DNA constructs including plant promoters capable of expressing RNA in plant cells. Such constructs may be used to inhibit production of ethylene-forming enzyme in transformed plants, and thereby to produce slower-ripening fruit, particularly melons. The clones may be obtained by PCR using specific oligo-nucleotide primers.
5486467 CATALASE FROM BACILLUS SUBTILIS IAM 1026 (FERM BP-4844) Fusho Yuichi; Yajima Kanagawa, JAPAN assigned Denko Kabushiki Kaisha
Yoshihiro to Showa
A microbial catalase having a catalase activity at 0 degrees C. of 95% or more of its catalase activity at 30 degrees C., when measured at pH 7,and a process for producing the same. The microbial catalase preferably has (1) an operative temperature of 0 degrees to 60 degrees C. and an optimum temperature of 0 degrees to 30
207
degrees C., (2) an optimum pH of 7 to 10, (3) a resistance to 10 mM potassium fluoride, (4) a molecular weight is 65,000+/-3,000, when measured by SDS polyacrylamide gel electrophoresis, and (5) an isoelectric point is about 4.8, when measured by isoelectric focusing. The catalase is obtainable from Bacillus subtilis IAM 1206 (FERM BP-4844) or a mutant strain thereof.
5487886 AMINO ACID BETA-LYASE ENZYME INHIBITORS AS DEODORANTS Lyon Sue B; O’Neal Cliffor; Van Der Lee Hermes; Rogers Brian Bethesda, MD, UNITED STATES assigned to The Gillette Company A deodorant composition comprising a body odor suppressing effective amount of an inhibitor of an amino acid B-lyase enzyme which catalyazes the formation of human body malodor, wherein the inhibitor is a compound of the formula NH2-C(CH3) (COOH)-CH2SR where R is a hydrogen, phenyl; C 1-C8 alkyl which is unsubstituted or substituted by a phenyl a hydroxy group, a benzyloxy or benzyloxycarbonyl group, a halogen or an amine group, in a dermatologically acceptable vehicle.
5487990 GLUCOSE-REGULATED PROMOTER OF YEAST ACETYL-COA HYDROLASE Smith John A; Lee Fang-Jen S; Lin Lee-We Scotch Plains, NJ, UNITED STATES assigned to The General Hospital Corporation
208
PATENT ABSTRACTS
This invention is directed to the acetyl-CoA hydrolase enzyme of yeast and to a method for purifying ace@-CoA hydrolase. The enzyme has a molecular weight of about 64,000 daltons and an enzyme activity of greater than 20 units wherein one unit of acetyl CoA hydrolase activity is defined as the amount of enzyme able to inhibit 1 unit of acetyltransferase activity and 1 unit of acetyltransferase activity is defined as the amount of enzyme needed to transfer 1 pmol of (3H) acetyl group from (3H) acetyl coenzyme A to ACTH (l-24) in the acetyltransferase standard enzyme assay under standard conditions. The invention is further directed to the glucose-repressible promoter which regulates the expression of the acetyl-CoA hydrolase gene in yeast, to vectors incorporating this promoter and to host cells transformed with such vectors.
5488062 2SACCHARINYLMETHYL HETEROCYCLIC CARBOXYLATES PROTEOLYTIC INHIBITORS
USEFUL ENZYME
AS
AND
COMPOSITIONS AND METHOD OF USE THEREOF Dunlap Richard; Hlasta Dennis; Desai Ranjit; Latimer Lee; Subramanyam Chakrapani; Court John J; Bell Malcolm R; Kumar Virendra Penfield, NY, UNITED STATES assigned to Sterling Winthrop Inc 4-R4-RS-2-Saccharinylmethyl heterocyclic carboxylates, useful in the treatment of degenerative diseases, are prepared by reacting a 4-R4-R5-2-halomethylsaccharin with either a heterocyclic carboxylic acid in the presence of an acid-acceptor or the alkali metal salt of a heterocyclic carboxylic acid.
5492813 MUTEINS
OF
BETA-GALACTOSIDASE FRAGMENTS HAVING INCREASED
ACTIVITY
Eisenbeis Scott J; Boguslawski Sophie J; Krevolin Mark; Ledden David J Indianapolis, IN, UNITED STATES assigned to Boehringer Mannheim Corporation Muteins of enzyme acceptor polypeptide fragments of beta-galactosidase are provided which exhibit substantially increased kinetic complementation activity with no significant loss in stability. A preferred enzyme acceptor fragment has an amino acid other than cysteine located at position 500 of the natural sequence. An especially preferred substitution is serine or valine. Other preferred muteins have an amino acid other than methionine located at position 443, with leucine being especially preferred, or an amino acid other than cysteine at position 76, with leucine being an especially preferred substitution. Also provided are methods for producing the novel muteins, reagent compositions comprising the novel muteins, and immunoassay methods for determining an analyte in which the novel mutein recombines with an enzyme donor polypeptide fragment to form enzymatically active beta-galactosidase. 5492822 METHOD FOR RECOVERING 100,000 DALTON PURIFIED MOLECULAR WEIGHT FRACTION OF HUMAN PANCREATIC CHOLESTEROL ESTERASE Lange Louis G; Spilburg Curtis A; St Louis, MO, Kinnunen Paula M