5498523 DNA SEQUENCING WITH PYROPHOSPHATASE Tabor Stanley; Richardson Charles Cambridge, MA, UNITED STATES assigned to President and Fellows of Harvard College A kit or solution for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule having a second single-stranded region homologous to the first single-stranded region, comprising a first agent able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture, and a second agent able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.
phage-infected bacteria; amplifying bacteriophage remaining in the sample; and causing the bacteriophage to infect reporter bacteria and thereby produce an observable signal. The reporter bacteria are genetically engineered to have an indicator gene which on expression gives rise to a detectable signal, wherein expression of the indicator gene is initiated on bacteriophage infection of the bacteria. 5498526 BACILLUS BIOLOGICAL GASEOUS
CIRCULANS INDICATOR STERILANTS
BASED FOR
Caputo Ross A; Martens Philhp A Long Grove, IL, UNITED STATES assigned to Abtox Inc Biological indicators are provided for use in validating and routinely monitoring oxidizing gas sterilizations. The biological indicators are based on Bacillus circular-is spores that are enclosed in packages with sufficient permeability to admit a sterilizing amount of vapor while being substantially bacteria impermeable.
5498525 5498527 METHODS MICROBIAL
FOR
RAPID
DETECTION
Rees Catherine E; Rostas-Mulligan Katalin; Park Simon F; Denyer Stephen P; Stewart Gordon S A B; Jassim Sabah A A Nottingham, UNITED KINGDOM assigned to Amersham International plc PCTNo. PCT/GB91/01354 Sec. 371 Date Apr. 9, 1992 Sec. 102(e) Date Apr. 9, 1992 PCT Filed Aug. 8, 1991 PCT Pub. No. WO92/02633 PCT Pub. Date Feb. 20, 1992.A method of testing for target bacteria involves adding bacteriophage to a sample to infect the bacteria in the sample; killing extracellular bacteriophage without at the same time killing
PHOSPHORYLCHOLINECONTAINING GLYCEROGLYCOLIPID Yamamoto Naok; Matsuda Kazuhir Tokyo, JAPAN assigned to Ube Industries Ltd The invention is directed to the compound I’-(2,3-Bis(hexadecanoyloxy)propyl)-d-g lycopyranos-6’-yl-2”-(trimethylammoniu which is a m)ethylphosphate phosphorylcholine-containing glyceroglycolipid useful for detecting the presence of Mycoplasma fermentans in cells or microorganisms.