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5500349 Blood-coagulation factor XIIa beta monoclonal antibody and immunoassay
PATENT ABSTRACTS
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susceptibility to alcoholism and cocaine dependence, particularly because said allele has been found to be present in a majority of clinically diagnosed alcoholics and cocaine users. The human D2 receptor gene Al and Bl alleles are most preferably detected in said sample.
5500344
SERINE PROTEASE AND USES THEREOF Sayers Thomas; Smyth Mark J; Wiltrout Theresa A; Powers James; Sowder Raymon; Henderson Louis E Boonsboro, MD, UNITED STATES assigned to The United States of America as represented by the Department of Health and Human Services The present invention provides a purified and isolated nucleic acid molecule encoding serine protease (Met-ase) having Met-ase activity but not Asp-ase activity and a molecular weight of about 30,000 daltons on SDS PAGE under reducing and non-reducing conditions. The present invention also provides a vector comprising this nucleic acid molecule, a prokaryotic or eukaryotic host cell stably transformed or transfected with the vector, as well as a method for detecting this nucleic acid in a sample.
PCT No. PCT/EP91/01407 Sec. 371 Date Jan. 27, 1993 Sec. 102(e) Date Jan. 27, 1993 PCT Filed Jul. 26, 1991 PCT Pub. No. WO92/02558 PCT Pub. Date Feb. 20, 1992. In a process for the purification of cytokeratin 20 (CK 20), a cytoskeletal fraction of cells containing CK 20 is produced, the proteins present therein are separated by gel electrophoresis or/and by chromatography and the CK 20 is isolated from the gel or the chromatographic fraction containing CK 20. In a process according to the present invention for the production of antibodies specific for CK 20, purified CK 20 is used for the immunization and then polyclonal or monoclonal antibodies are produced according to well-known methods. These antibodies are used for the immunologically identification of CK 20, or its alpha-helical central fragment obtained by proteolytic cleavage on tissue sections, in tissue homogenates and in body fluids.
5500349 BLOOD-COAGULATION FACTOR XIIA BETA MONOCLONAL ANTIBODY AND IMMUNOASSAY
5500347 Esnouf Michael P Combe, UNITED KINGDOM assigned to Coagen Limited
METHOD FOR THE PURIFICATION OF CYTOKERATIN 20 AND ITS USE FOR THE PRODUCTION OF ANTIBODIES Moll Roland; Franke Heidelberg, GERMANY Progen Biotechnik GmbH
Werner assigned
W to
A monoclonal antibody which binds to Factor betaXI and which shows substantially no binding to Factor XII. This antibody may be used in immunoassays to measure Factor betaXIIa levels in blood, for example for studies of blood coagulation systems and of thrombotic disorders.
188
susceptibility to alcoholism and cocaine dependence, particularly because said allele has been found to be present in a majority of clinically diagnosed alcoholics and cocaine users. The human D2 receptor gene Al and Bl alleles are most preferably detected in said sample.
5500344
SERINE PROTEASE AND USES THEREOF Sayers Thomas; Smyth Mark J; Wiltrout Theresa A; Powers James; Sowder Raymon; Henderson Louis E Boonsboro, MD, UNITED STATES assigned to The United States of America as represented by the Department of Health and Human Services The present invention provides a purified and isolated nucleic acid molecule encoding serine protease (Met-ase) having Met-ase activity but not Asp-ase activity and a molecular weight of about 30,000 daltons on SDS PAGE under reducing and non-reducing conditions. The present invention also provides a vector comprising this nucleic acid molecule, a prokaryotic or eukaryotic host cell stably transformed or transfected with the vector, as well as a method for detecting this nucleic acid in a sample.
PCT No. PCT/EP91/01407 Sec. 371 Date Jan. 27, 1993 Sec. 102(e) Date Jan. 27, 1993 PCT Filed Jul. 26, 1991 PCT Pub. No. WO92/02558 PCT Pub. Date Feb. 20, 1992. In a process for the purification of cytokeratin 20 (CK 20), a cytoskeletal fraction of cells containing CK 20 is produced, the proteins present therein are separated by gel electrophoresis or/and by chromatography and the CK 20 is isolated from the gel or the chromatographic fraction containing CK 20. In a process according to the present invention for the production of antibodies specific for CK 20, purified CK 20 is used for the immunization and then polyclonal or monoclonal antibodies are produced according to well-known methods. These antibodies are used for the immunologically identification of CK 20, or its alpha-helical central fragment obtained by proteolytic cleavage on tissue sections, in tissue homogenates and in body fluids.
5500349 BLOOD-COAGULATION FACTOR XIIA BETA MONOCLONAL ANTIBODY AND IMMUNOASSAY
5500347 Esnouf Michael P Combe, UNITED KINGDOM assigned to Coagen Limited
METHOD FOR THE PURIFICATION OF CYTOKERATIN 20 AND ITS USE FOR THE PRODUCTION OF ANTIBODIES Moll Roland; Franke Heidelberg, GERMANY Progen Biotechnik GmbH
Werner assigned
W to
A monoclonal antibody which binds to Factor betaXI and which shows substantially no binding to Factor XII. This antibody may be used in immunoassays to measure Factor betaXIIa levels in blood, for example for studies of blood coagulation systems and of thrombotic disorders.