5501954 Method of detecting cellular material

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PATENT ABSTRACTS

oligonucleotide probes for detecting Coccidioides immitis. The probes are capable of distinguishing between Coccidioides immitis and closely related phylogenetic neighbors. These probes are targeted to unique Coccidioides immitis 28s rRNA and gene sequences encoding 28s rRNA, and may be used in an assay for the detection and/or quantitation of Coccidioides immitis, e.g., in samples of sputum, urine, blood, tissue sections, food, soil and water. 5501953 PROCESS QUANTITATIVELY LIPOSOMES FOR

FOR LYSING

AND A PROCESS

DETERMINING

AMOUNT

THE

OF AN ANALYTE

USING

The method of the present invention relates to a rapid procedure for detecting DNA in a cell, while preserving the morphology of the nucleus for analysis. The method comprises depositing a cell onto a polymeric membrane filter wherein the DNA contained in the cell is retained on the polymeric membrane filter and is available for binding with a fluorescently labeled nucleic acid probe, incubating the polymeric membrane filter with the labeled nulceic acid probe, and detecting the labeled nulceic acid probe wherein detection of the nulceic acid probe is indicative of the presence of the DNA. There are no separate permeabilization steps needed to permit the probes to enter the cell and hybridize to the DNA. The method is simple and quick and is applicable to the sample volumes found in clinical laboratories.

SAME 5501957

Fujita Minoru; Kida Masaaki Amagasaki, JAPAN assigned to Wako Pure Chemical Industries Ltd Membranes of liposomes fixing biotin on membrane surfaces thereof can be lysed by a mixed agent comprising a surfactant and avidin. This mechanism can be applied to immunoassay for determining an analyte, e.g. antigen or antibody, wherein liposomes encapsulating a marker and fixing biotin on membrane surfaces thereof are lysed depending on hindrance of an avidin-biotin binding reaction by a specific reaction caused by the analyte. 5501954 METHOD OF DETECTING CELLULAR MATERIAL Mahr Anna M; Bowe Ann E; Rut&Roberts Alyson; Klinger Katherine W Natick, MA, UNITED STATES assigned to Genzyme Corporation

METHOD FOR MEASURING GLYCOSYLTRANSFERASE ACTIVITY Dennis James; Siminovitch Katherine A; Datti Alessandro Etobicoike, CANADA assigned to Mount Sinai Hospital Corporation A method of assaying for glycosyltransferase activity in a sample. In a first step, a sample is reacted with a first sugar donor and an acceptor substrate to produce a transferase product. The first sugar donor and acceptor substrate are selected such that the sugar from the first sugar donor is capable of being transferred to the acceptor substrate in the presence of the glycosyltransferase to be assayed. In a second step, the transferase product is reacted with a second sugar donor having a sugar which is labelled with a labelling agent and an enzyme which is capable of transferring the sugar from the second sugar donor to the transferase product to