5547850 Process for determining hirudin and synthetic thrombin inhibitors

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PATENT ABSTRACTS

5547846 IMMUNOGENIC REGIONS ON THE E7 PROTEIN OF HUMAN PAPILLOMAVIRUS T Y P E 16 Bartsch Dusa; Gissmann I.at; Muml uller Martin Heidelberg, GERMANY Assigned to Behringwerke Aktiengeseilschaft The invention describes two immunogenic regions of the E7 protein of human papillomavirus type 16 (HPV 16), one immtmoreactive region being located downstream from nucleotide 595 and the other being located downstream from nucleotide 667 of the HPV 16 genome.

5547848 IMMUNOASSAY ELEMENT CONTAINING A PULVERIZED WATER-INSOLUBLE POLYSACCHARIDE AND PROCESS FOR IMMUNOASSAY Shinoki Hiroshi; Hiraoka Toshikage; Ogawa Masashi Saitama, JAPAN Assigned to Fuji Photo Film Co Ltd An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity. When the ligand is a low molecular weight antigen, competitive reactions between the ligand, enzyme-labelled antibody and conjugate of the antigen and high molecular weight compound are utilized. When the ligand is a macromolecular antigen, a reaction between the ligand and an enzyme-labelled antibody is utilized directly. The immonoassay element comprises a substrate layer containing a non-diffusible subsuate which forms a diffusible material in the presence of the enzyme, and a reagent layer for detecting the thus fomaed diffusible material. The non-diffusible substrate is composed of a pulverized insoluble polysaccharide. The reagent layer may further contain a fragmenting enzyme for further fragmenting the non-diffusible material. Also provided are processes for quantitatively analyzing both of low molecular weight and macromolecular antigens contained in any samples by the use of the immunoassay

elements of the invention.

5547850 PROCESS

FOR DETERMINING

HIRUDIN AND SYNTHETIC THROMBIN INHIBITORS Nowak Guml otz; Bucha Elke; Hoffman Jutta Erfurt, GERMANY Assigned to M ax-Planck-Gesellschaft PCT No. PCT/EP93/00161 Sec. 371 Date Oct. 5, 1994 Sec. 102(e) Date Oct. 5, 1994 PCT Filed Jan. 25, 1993 PCT Pub. No. WO93/16390 PCT Pub. Date Aug. 19, 1993. The present invention provides a process for determining the content of hirudin or of a synthetic thrombin inhibitor in the blood. A prothrombin intermediate, or a compound that splits prothrombin into meizothrombin, or a mixture thereof, is added to a blood sample and a measurement is taken of the time that elapses between the addition of the reagent to the sample and the beginning of coagulation. Comparison is then made, where necessary, with a standard calibration curve.

5547851 METHOD FOR MEASURING CARBON DIOXIDE AND REAGENT THEREFOR Hattori Shizuo; Kishimoto Takahide; Sogabe Yukihiro; Emi Shigenori Tsuruga, JAPAN Assigned to Toyo Boseki Kabushiki Kaisha A method for measuring carbon dioxide, comprising the steps of: (1) reacting bicarbonate ion in a sample with phosphoenolpyruvate carboxylese derived from an acetic acid bacterium in the presence of phosphoenolpyruvate; (2) reacting the resultant oxalacetic acid with malate dehydrogenase in the presence of NADH; and (3) measuring decreased NADH, and a reagent for measuring carbon dioxide, comprising phosphoenolpyruvate, phosphoenolpyruvate carboxylase derived from an acetic acid bacterium, malate dehydrogenase, NADH and a buffer. According to the present invention, a highly stable reagent for CO2 me~urement, permitting a long-term storage in a liquid state can