- Email: [email protected]
5554503 Sample processing method for mycobacterium tuberculosis
PATENT ABSTRACTS
oligonucleotides thereto, and these devices are most preferably utifized for genetic analysis of patient samples.
435
from the processed pellet without significant loss of microorganisms by at least two washings with saline, water, or buffers compatible with nucleic acid analysis and appropriate centrifugation.
5554502 5554507 PROCESS FOR DETERMINING NUCLEASE ACTIVITY Mitsuhashi Masato; Ogura Mieko Irvine, CA, UNFTED STATES Assigned to Hitachi Chemical Co Ltd; Hitachi Chemical Research Center Inc The present invention relates to analyzing a test sample for the presence or absence of nuclease activity, and for quantifying the amount of nuclease activity. In one aspect of the invention, a kit is provided which allows the rapid and efficient quantification of nuclease activity in a sample having an unknown nuclease activity. In another aspect of the invention, a method for determining the presence or absence of nuclease activity, and for quantifying nuclense activity is disclosed.
BACILLUS SUBTILIS SIDEROPHORE GENES Grossman Tmdy H; Osbume Marcia S Mount Kisco, NY, UNITED STATES Assigned to American Cyanamid Company An isolated nucleic acid sequence is described having at least one siderophore biosynthetic gene from Bacillus subtilis encoding enzymes homologous to enterobactin enzymes EntA, EntB, EntC, EntE, and combinations thereof. Vectors containing the isolated nucleic acid sequence, host cells transformed wth the isolated nucleic acid sequence, and a method for identifying compounds having antibiotic activity against microorganisms with an enterobactin-type siderophore are also described.
5554503 5554509 SAMPLE PROCESSING METHOD FOR MYCOBACTERIUM TUBERCULOSIS Down James A; Waiters Adriann H; Dey Margaret S; Howard Deborah R; Little Michael C; Keating William E Cary, NC, UNITED STATES Assigned to Becton Dickinson and Company Methods for processing samples which may contain mycobacteria which are compatible with both conventional culturing techniques and nucleic acid analysis. It has been found that the harsh chemical treatment previously thought necessary to obtain efficient lysis can be eliminated without loss of lysis efficiency, thereby eliminating reagents which may inhibit subsequent nucleic acid-based reactions. Heat alone is sufficient to lyse mycobacteria, even in buffers which do not contain detergents, chelators, enzymes, etc. Residual reagents introduced by conventional sample processing methods for culture can be successfully removed
NUCLEOTIDE PROBES AND METHODS FOR DETERMINING TAQI POLYMORPHISMS IN THE HUMAN APO(A) GENE Colucci Giuseppe; Taramelli Roberto ITALY Assigned to Clonit SpA
Milan,
A polynucleotide probe capable of hybridizing to human genomic DNA between the Taql sites immediately 5' and 3' of the polymorphic leader TaqI site of the human Aim(a) gene, said human genomic DNA being detectable by PCR amplification of the Apo(a) gene using the 5' and 3' primers: 5':5'CCT ATT TGG ATr TI'G GAC GC 3'(SEQ ID NO. l) 3':5~3ATAAC AGA CCA ATA GCT GT 3'. (SEQ ID NO. 2), or a polynuclootide probe capable of hybridizing to human genomic DNA between the TaqI sites immediately 5' and 3' of the polymorpliic kringle TaqI site of the human Apo(a) gene, said human genomic DNA being detectable by PCR amplification of the Apo(a) geue using the 5' and 3' primers: 5':5'CTG CA(] ACA ACC CCT TAA
oligonucleotides thereto, and these devices are most preferably utifized for genetic analysis of patient samples.
435
from the processed pellet without significant loss of microorganisms by at least two washings with saline, water, or buffers compatible with nucleic acid analysis and appropriate centrifugation.
5554502 5554507 PROCESS FOR DETERMINING NUCLEASE ACTIVITY Mitsuhashi Masato; Ogura Mieko Irvine, CA, UNFTED STATES Assigned to Hitachi Chemical Co Ltd; Hitachi Chemical Research Center Inc The present invention relates to analyzing a test sample for the presence or absence of nuclease activity, and for quantifying the amount of nuclease activity. In one aspect of the invention, a kit is provided which allows the rapid and efficient quantification of nuclease activity in a sample having an unknown nuclease activity. In another aspect of the invention, a method for determining the presence or absence of nuclease activity, and for quantifying nuclense activity is disclosed.
BACILLUS SUBTILIS SIDEROPHORE GENES Grossman Tmdy H; Osbume Marcia S Mount Kisco, NY, UNITED STATES Assigned to American Cyanamid Company An isolated nucleic acid sequence is described having at least one siderophore biosynthetic gene from Bacillus subtilis encoding enzymes homologous to enterobactin enzymes EntA, EntB, EntC, EntE, and combinations thereof. Vectors containing the isolated nucleic acid sequence, host cells transformed wth the isolated nucleic acid sequence, and a method for identifying compounds having antibiotic activity against microorganisms with an enterobactin-type siderophore are also described.
5554503 5554509 SAMPLE PROCESSING METHOD FOR MYCOBACTERIUM TUBERCULOSIS Down James A; Waiters Adriann H; Dey Margaret S; Howard Deborah R; Little Michael C; Keating William E Cary, NC, UNITED STATES Assigned to Becton Dickinson and Company Methods for processing samples which may contain mycobacteria which are compatible with both conventional culturing techniques and nucleic acid analysis. It has been found that the harsh chemical treatment previously thought necessary to obtain efficient lysis can be eliminated without loss of lysis efficiency, thereby eliminating reagents which may inhibit subsequent nucleic acid-based reactions. Heat alone is sufficient to lyse mycobacteria, even in buffers which do not contain detergents, chelators, enzymes, etc. Residual reagents introduced by conventional sample processing methods for culture can be successfully removed
NUCLEOTIDE PROBES AND METHODS FOR DETERMINING TAQI POLYMORPHISMS IN THE HUMAN APO(A) GENE Colucci Giuseppe; Taramelli Roberto ITALY Assigned to Clonit SpA
Milan,
A polynucleotide probe capable of hybridizing to human genomic DNA between the Taql sites immediately 5' and 3' of the polymorphic leader TaqI site of the human Aim(a) gene, said human genomic DNA being detectable by PCR amplification of the Apo(a) gene using the 5' and 3' primers: 5':5'CCT ATT TGG ATr TI'G GAC GC 3'(SEQ ID NO. l) 3':5~3ATAAC AGA CCA ATA GCT GT 3'. (SEQ ID NO. 2), or a polynuclootide probe capable of hybridizing to human genomic DNA between the TaqI sites immediately 5' and 3' of the polymorpliic kringle TaqI site of the human Apo(a) gene, said human genomic DNA being detectable by PCR amplification of the Apo(a) geue using the 5' and 3' primers: 5':5'CTG CA(] ACA ACC CCT TAA