5558985 Stable hemoglobin reference solution

446

PATENT ABSTRACTS

5558985

5558987

STABLE HEMOGLOBIN REFERENCE SOLUTION

BLOOD LEVELS OF CCK PEPTIDES RELATIVE TO PANIC

Chiang Ching; Liffman Stanley M Acton, MA, UNITED STATES Assigned to Bionostics Incorporated A reference solution for calibrating and monitoring blood gas instrumentation that has a shelf line of two years or greater is disclosed. The solution is contained in a closed container having a gas or gas mixture that is inert to the reference solution and comprises an aqueous mixture containing a hemoglobin, a source of bicarbonate ions and an organic buffer. The solution optionally contains a methemoglobin reducing system, reference concentrations of selected electrolytes, a polyphosphate compound and antibiotics. The ampule is subsequently opened and the solution is equilibrated with a gas mixture having components which provide gasses in a physiological range to the solution. The solution is characterized by exhibiting properties similar to fresh blood subsequent to equilibration.

DISORDER

TREATMENT Thomas Thomas; Sheehan David; Talbot Janet Palm Harbor, FL UNITED STATES Assigned to University of South Florida A method of treating a patient having a panic disorder, the patient having an elevated CCK peptide plasma level, by lowering the plasma CCK peptide level of the patient. A further method provides a diagnosis of panic disorder in a patient by detecting if that patient's plasma contains elevated CCK peptide levels. A further method determines the efficacy of the drug for the treatment of panic disorder by detecting the ability of the drug to lower elevated CCK peptide levels in a model for panic disorder. Additionally, a method of dosing a patient having elevated CCK peptide levels with an antipanic disorder drug is characterized by administering the drug to a patient and monitoring the lowering of the elevated plasma CCK peptide levels of the patient.

5558988 5558986 METHOD

AND KIT FOR

PRIMERS ATP

ASSAYS USING CYCLODEXTRIN Lundin Anne Dalaro, SWEDEN Assigned to Merck Patent GmbH A method of preparing an extract of an intracellular component, wherein cells are contacted with an extractant to generate an extract solution, which comprises contacting the solution with a cyclodextrin to neutralize the extractant. preferred extractants are cationic or other kinds of surfactants. The cyclodextrin is preferably used in solution. Preferred intracellular components are ATP which can, after neutralization of the extractant, be assayed by a firefly luciferase assay; and DNA or RNA which can, after neutralization of the surfactant, be amplified or further processed in other ways.

AND METHODS

FOR

DETECTING MUTATIONS IN THE PROCOLLAGEN II GENE THAT INDICATE A GENETIC PREDISPOSITION FOR OSTEOARTHRITIS Prockop Darwin J; Ala-Kokko Leen; Ritvaniemi Pertti Philadelphia, PA, UNITED STATES Assigned to Thomas Jefferson University The invention provides probes and primers for amplifying certain regions of genes for structural proteins of cartilage and methods for detecting mutations in these genes isolated from the nucleic acid of cells suspected of exhibiting mutant structural protein gene expression or having mutant structural protein genes. The invention also provides methods for determining a genetic predisposition for a disease that alters the structure or function of cartilage because of a