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5567581 Method and kit for enzymatically determining the pH of a specimen
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PATENT ABSTRACTS
5565352
5567581
DEUBIQUITINATING ENZYME: C O M P O S I T I O N S AND METHODS
M E T H O D AND KIT FOR ENZYMATICALLY DETERMINING THE PH OF A SPECIMEN
Hochstrasser Mark; Papa Feroz Arch Development Corporation
Assigned to
The invention relates generally to compositions of and methods for obtaining deubiquitinating enzyme polypeptides. The invention relates as well to polynucleotides encoding deubiquitinating enzyme polypeptides, the recombinant vectors carrying those sequences, the recombinant host cells including either the sequences or vectors, and recombinant deubiquitinating enzyme polypeptides. By way of example, the invention discloses the cloning and functional expression if at least three different deubiquitinating enzyme polypeptides. The invention includes as well, methods for using the isolated, recombinant enzyme polypeptides in assays designed to select and improve substances capable of interacting with deubiquitinating enzyme polypeptides for use in diagnostic, drug design and therapeutic applications.
5565430 AZAASPARTIC ACID A N A L O G S AS INTERLEUKIN-1 BETA CONVERTING ENZYME INHIBITORS Dolle Roland E; Graybill Todd L King of Prussia, PA, UNITED STATES Assigned to Sterling Winthrop lnc Disclosed are compounds, compositions and methods for inhibiting interleukinprotease activity. The compounds, alpha-substituted acetamides a (*See Patent for Chemical Structure*) (A) wherein: R2=H or alkyl; R3=halo, O(CO)0-1 aryl, OPOR4R5; (*See Patent for Chemical Structure*) where R4 and R5=aryl; R6=H, aryl or aralkyl; R7--independantly selected from R6, CF3 and CF2CF3; RI=R6-CO, heteroaryl-CO, heteroaralkyI-CO and amino acid.
Lin Cheng-; Tsay Yuh-Gen Cupertino, CA, UNITED STATES Assigned to Diagnostic Reagents lnc The present invention concerns a method of enzymatically determining the pH of a specimen (e.g., a solution or a biological fluid) and a kit for conducting the method. The present method involves mixing (1) a specimen with (2) an enzyme and (3) one or more substrates for the enzyme in a buffered solution having a pH effective to provide a direct proportional relationship between the activity of the enzyme and the pH of the specimen; determining the activity of the enzyme; and correlating the activity of the enzyme to the pH of the specimen. Each of the sample, the enzyme, the substrate and the buffered solution is present in an amount effective to provide the direct proportional relationship between the activity of the enzyme and the pH of the specimen. The present kit contains an enzyme, one or more substrates for the enzyme present in an amount effective to determine the enzyme activity, and a buffered solution having a pH effective to provide a direct proportional relationship between the activity of the enzyme and the pH of the specimen. The present invention is particularly applicable to detecting adulteration of a urine specimen.
5567597 H Y D R O L Y T I C ENZYME INHIBITORS/INACTIVATORS AND M E T H O D S FOR USING SAME Dennis Edward A; Washburn William N LaJolla, CA, UNITED STATES Assigned to The Regents of the University of California The present invention provides compounds that function as hydrolytic enzyme inhibiturs (inactivators) and substrates. These compounds
PATENT ABSTRACTS
5565352
5567581
DEUBIQUITINATING ENZYME: C O M P O S I T I O N S AND METHODS
M E T H O D AND KIT FOR ENZYMATICALLY DETERMINING THE PH OF A SPECIMEN
Hochstrasser Mark; Papa Feroz Arch Development Corporation
Assigned to
The invention relates generally to compositions of and methods for obtaining deubiquitinating enzyme polypeptides. The invention relates as well to polynucleotides encoding deubiquitinating enzyme polypeptides, the recombinant vectors carrying those sequences, the recombinant host cells including either the sequences or vectors, and recombinant deubiquitinating enzyme polypeptides. By way of example, the invention discloses the cloning and functional expression if at least three different deubiquitinating enzyme polypeptides. The invention includes as well, methods for using the isolated, recombinant enzyme polypeptides in assays designed to select and improve substances capable of interacting with deubiquitinating enzyme polypeptides for use in diagnostic, drug design and therapeutic applications.
5565430 AZAASPARTIC ACID A N A L O G S AS INTERLEUKIN-1 BETA CONVERTING ENZYME INHIBITORS Dolle Roland E; Graybill Todd L King of Prussia, PA, UNITED STATES Assigned to Sterling Winthrop lnc Disclosed are compounds, compositions and methods for inhibiting interleukinprotease activity. The compounds, alpha-substituted acetamides a (*See Patent for Chemical Structure*) (A) wherein: R2=H or alkyl; R3=halo, O(CO)0-1 aryl, OPOR4R5; (*See Patent for Chemical Structure*) where R4 and R5=aryl; R6=H, aryl or aralkyl; R7--independantly selected from R6, CF3 and CF2CF3; RI=R6-CO, heteroaryl-CO, heteroaralkyI-CO and amino acid.
Lin Cheng-; Tsay Yuh-Gen Cupertino, CA, UNITED STATES Assigned to Diagnostic Reagents lnc The present invention concerns a method of enzymatically determining the pH of a specimen (e.g., a solution or a biological fluid) and a kit for conducting the method. The present method involves mixing (1) a specimen with (2) an enzyme and (3) one or more substrates for the enzyme in a buffered solution having a pH effective to provide a direct proportional relationship between the activity of the enzyme and the pH of the specimen; determining the activity of the enzyme; and correlating the activity of the enzyme to the pH of the specimen. Each of the sample, the enzyme, the substrate and the buffered solution is present in an amount effective to provide the direct proportional relationship between the activity of the enzyme and the pH of the specimen. The present kit contains an enzyme, one or more substrates for the enzyme present in an amount effective to determine the enzyme activity, and a buffered solution having a pH effective to provide a direct proportional relationship between the activity of the enzyme and the pH of the specimen. The present invention is particularly applicable to detecting adulteration of a urine specimen.
5567597 H Y D R O L Y T I C ENZYME INHIBITORS/INACTIVATORS AND M E T H O D S FOR USING SAME Dennis Edward A; Washburn William N LaJolla, CA, UNITED STATES Assigned to The Regents of the University of California The present invention provides compounds that function as hydrolytic enzyme inhibiturs (inactivators) and substrates. These compounds