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5580726 Method and kit for enhanced differential display
476
PATENT ABSTRACTS
protein of interest associated with treatment of one or more symptoms of a cardiovascular disease. Lastly, the invention provides a method of directly and specifically transcriptionally modulating in a human being the expression of a gene encoding a protein of interest associated with treatment of one or more symptoms of a cardiovascular disease, thus ameliorating the disease.
21 oligonucleotides. The method further comprises using a two-step PCR amplification, wherein non-stringent conditions are used for the first 1 to 4 cycles, and stringent conditions are used for the next 16 to 22 cycles. This highly reproducible method will permit the preparation of comprehensive catalogs of gene expression for any given cell type.
5580727 5580725 M E T H O D OF ELIMINATING GENETIC ROUTES FOR BACTERIOPHAGE EVOLUTION AND PRODUCTS PRODUCED THEREBY Klaenhammer Todd R; Moineau Sylvai Raleigh, NC, UNITED STATES Assigned to North Carolina State Universtiy A process of identifying and disrupting bacterial DNA sequences that contribute to the evolution of new lytic bacteriophages is described. Vectors and recombinant bacteria for use in producing fermentative starter cultures and culture media resistant to the appearance of new phages, and methods of producing such vectors and recombinant bacteria, are described.
5580726 M E T H O D A N D KIT FOR E N H A N C E D DIFFERENTIAL DISPLAY Villeponteau Bryant; Feng Junli; Funk Walte; Linskens Maarten H K San Carlos, CA, UNITED STATES Assigned to Geron Corporation An improved method for detecting and isolating differentially expressed mRNAs which comprises using first oligonucleotide primers for reverse transcription of mRNAs and both the first oligonucleotide primers and second oligonucleotide primers for amplification of the resultant cDNAs. The improvement of this method comprises providing first and second oligonucleotide primers with a length of at least
AMLI-MTG8 FUSION PROTEIN RESULTING FROM T(8;21) T R A N S L O C A T I O N IN ACUTE MYELOID L E U K E M I A Ohki Misao; Kikuchi Kimiko; Miyoshi Hiroyuki; Kozu Tomoko Kokubunji, JAPAN Assigned to SRL Inc Means for diagnosing t(8;21) translocation type acute myeloid leukemia with high sensitivity by a simple operation are disclosed. The present invention provided AMLI-MTG8 fused DNA having the nucleotide sequence shown in SEQ 1D No. 1 and DNA fragments thereof containing the fused site. The present invention provided AMLI-MTG8 fused polypeptide having the amino acid sequence shown in SEQ ID No. 2, and fragments thereof containing the fused site. The present invention provided AMLI-MTG8 fused mRNA having the same nucleotide sequence as shown in SEQ ID No. I except that thymine is replaced with uracil, and fragments thereof. The present invention provided a probe which is said DNA fragment that is labelled. The present invention provided a method for detecting said fused DNA by using said probe. The present invention provided a method for detecting AML1-MTG8 fused mRNA or a fragment thereof in a sample, comprising the steps of amplifying said fused DNA or a fragment thereof by a nucleic acid-amplifying method and detecting the amplified DNA fragment.
5580728 M E T H O D A N D SYSTEM FOR GENOTYPING Perlin Mark W STATES
Pittsburgh, PA, UNITED
PATENT ABSTRACTS
protein of interest associated with treatment of one or more symptoms of a cardiovascular disease. Lastly, the invention provides a method of directly and specifically transcriptionally modulating in a human being the expression of a gene encoding a protein of interest associated with treatment of one or more symptoms of a cardiovascular disease, thus ameliorating the disease.
21 oligonucleotides. The method further comprises using a two-step PCR amplification, wherein non-stringent conditions are used for the first 1 to 4 cycles, and stringent conditions are used for the next 16 to 22 cycles. This highly reproducible method will permit the preparation of comprehensive catalogs of gene expression for any given cell type.
5580727 5580725 M E T H O D OF ELIMINATING GENETIC ROUTES FOR BACTERIOPHAGE EVOLUTION AND PRODUCTS PRODUCED THEREBY Klaenhammer Todd R; Moineau Sylvai Raleigh, NC, UNITED STATES Assigned to North Carolina State Universtiy A process of identifying and disrupting bacterial DNA sequences that contribute to the evolution of new lytic bacteriophages is described. Vectors and recombinant bacteria for use in producing fermentative starter cultures and culture media resistant to the appearance of new phages, and methods of producing such vectors and recombinant bacteria, are described.
5580726 M E T H O D A N D KIT FOR E N H A N C E D DIFFERENTIAL DISPLAY Villeponteau Bryant; Feng Junli; Funk Walte; Linskens Maarten H K San Carlos, CA, UNITED STATES Assigned to Geron Corporation An improved method for detecting and isolating differentially expressed mRNAs which comprises using first oligonucleotide primers for reverse transcription of mRNAs and both the first oligonucleotide primers and second oligonucleotide primers for amplification of the resultant cDNAs. The improvement of this method comprises providing first and second oligonucleotide primers with a length of at least
AMLI-MTG8 FUSION PROTEIN RESULTING FROM T(8;21) T R A N S L O C A T I O N IN ACUTE MYELOID L E U K E M I A Ohki Misao; Kikuchi Kimiko; Miyoshi Hiroyuki; Kozu Tomoko Kokubunji, JAPAN Assigned to SRL Inc Means for diagnosing t(8;21) translocation type acute myeloid leukemia with high sensitivity by a simple operation are disclosed. The present invention provided AMLI-MTG8 fused DNA having the nucleotide sequence shown in SEQ 1D No. 1 and DNA fragments thereof containing the fused site. The present invention provided AMLI-MTG8 fused polypeptide having the amino acid sequence shown in SEQ ID No. 2, and fragments thereof containing the fused site. The present invention provided AMLI-MTG8 fused mRNA having the same nucleotide sequence as shown in SEQ ID No. I except that thymine is replaced with uracil, and fragments thereof. The present invention provided a probe which is said DNA fragment that is labelled. The present invention provided a method for detecting said fused DNA by using said probe. The present invention provided a method for detecting AML1-MTG8 fused mRNA or a fragment thereof in a sample, comprising the steps of amplifying said fused DNA or a fragment thereof by a nucleic acid-amplifying method and detecting the amplified DNA fragment.
5580728 M E T H O D A N D SYSTEM FOR GENOTYPING Perlin Mark W STATES
Pittsburgh, PA, UNITED