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5583034 Enhancement of adoptosis using antisense oligonucleotides
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PATENT ABSTRACTS
A method of using a chimeric molecule made up of an antisense oligonucleotide attached to a 2',5'-oligoadenylate molecule to specifically cleave a sense strand of RNA, wherein the antisense oligonucleotide of the chimeric molecule is hybridized to the sense strand of RNA in the presence of 2',5'-dependent Rnase.
5583034 E N H A N C E M E N T OF A D O P T O S I S USING ANTISENSE OLIGONUCLEOTIDES Green Douglas; Cotter Thomas San Diego, CA, UNrIED STATES Assigned to La Julia Institute for Allergy and Immunology A method is disclosed for enhancement of the efficacy of therapeutic treatment for inducing cell death in a cell having an anti-apoptotic gene by the enhancement of apoptosis. An antisense oligonucleotide is disclosed which hybridizes with the nucleic acid sequence of the anti-apoptotic gene. The oligonucleotide is administered to the cell in an amount sufficient to inhibit expression of the gene, thus rendering the cell susceptible to induction of apoptosis, and consequently achieving higher efficacy of therapeutic treatment.
5583037 TRANS-MEMBRANE C O - C U L T U R E INSERT A N D M E T H O D F O R USING Mussi Edward F; Macchi William A Hewitt, NJ, UNITED STATES Assigned to Becton Dickinson and Company An insert for trans-membrane co-culture includes a hollow housing with a passageway therethrough, a first end, a second end and a center section intermediate the first and second end. The passageway has an inside diameter at the f'u:stend and the second end that is larger than the inside diameter of the passageway at the center section so that the passageway tapers from each end to the center section. The center section includes a porous membrane having a first surface and a second surface attached across and obstructing the passageway. The membrane forms a first
receptacle from the passageway toward the ftrst end and forms a second receptacle from the passageway toward the second end. A method for using the insert of the present invention to grow cells on both sides of a porous membrane includes horizontally submerging the insert in liquid medium so that the air in the receptacles is displaced by the medium. The insert is then tipped to an upright position while maintaining one end below the surface of the liquid. The insert is stood upright on the bottom of the well and a first cell suspension introduced in to the first receptacle. After allowing the first cell suspension to form a layer of cells on the first surface of the membrane the insert is removed, drained and again horizontally immersed in liquid medium so that the receptacles are filled. The insert is then returned to vertical while maintaining the first end below the surface of the medium, stood on the first end and a second cell suspension is introduced into the second receptacle. After suitable incubation, the second cell population forms a layer on the second surface of the membrane.
5583042 A P P A R A T U S FOR THE SYNTHESIS OF S A C C H A R I D E COMPOSITIONS Roth Stephe Gladwyne, PA, UNITED STATES Assigned to NEOSE Pharmaceuticals Inc This invention relates to an apparatus containing specific binary combinations of glycosyltransferases, for the synthesis of specific saccharide compositions such as, for example, oligosaccharides, polysaccharides, glycolipids, and glycopeptides.
5583043 C O V E R SLIP H O L D E R FOR BILATERAL A N D U N I L A T E R A L S I M U L A T I O N OF PARTLY T H R O M B O G E N E S I S IN O C C L U D E D B L O O D VESSELS AND USE T H E R E O F Sakariassen Kjell S Oslo, NORWAY Assigned to Hafslund Nycomed As
PATENT ABSTRACTS
A method of using a chimeric molecule made up of an antisense oligonucleotide attached to a 2',5'-oligoadenylate molecule to specifically cleave a sense strand of RNA, wherein the antisense oligonucleotide of the chimeric molecule is hybridized to the sense strand of RNA in the presence of 2',5'-dependent Rnase.
5583034 E N H A N C E M E N T OF A D O P T O S I S USING ANTISENSE OLIGONUCLEOTIDES Green Douglas; Cotter Thomas San Diego, CA, UNrIED STATES Assigned to La Julia Institute for Allergy and Immunology A method is disclosed for enhancement of the efficacy of therapeutic treatment for inducing cell death in a cell having an anti-apoptotic gene by the enhancement of apoptosis. An antisense oligonucleotide is disclosed which hybridizes with the nucleic acid sequence of the anti-apoptotic gene. The oligonucleotide is administered to the cell in an amount sufficient to inhibit expression of the gene, thus rendering the cell susceptible to induction of apoptosis, and consequently achieving higher efficacy of therapeutic treatment.
5583037 TRANS-MEMBRANE C O - C U L T U R E INSERT A N D M E T H O D F O R USING Mussi Edward F; Macchi William A Hewitt, NJ, UNITED STATES Assigned to Becton Dickinson and Company An insert for trans-membrane co-culture includes a hollow housing with a passageway therethrough, a first end, a second end and a center section intermediate the first and second end. The passageway has an inside diameter at the f'u:stend and the second end that is larger than the inside diameter of the passageway at the center section so that the passageway tapers from each end to the center section. The center section includes a porous membrane having a first surface and a second surface attached across and obstructing the passageway. The membrane forms a first
receptacle from the passageway toward the ftrst end and forms a second receptacle from the passageway toward the second end. A method for using the insert of the present invention to grow cells on both sides of a porous membrane includes horizontally submerging the insert in liquid medium so that the air in the receptacles is displaced by the medium. The insert is then tipped to an upright position while maintaining one end below the surface of the liquid. The insert is stood upright on the bottom of the well and a first cell suspension introduced in to the first receptacle. After allowing the first cell suspension to form a layer of cells on the first surface of the membrane the insert is removed, drained and again horizontally immersed in liquid medium so that the receptacles are filled. The insert is then returned to vertical while maintaining the first end below the surface of the medium, stood on the first end and a second cell suspension is introduced into the second receptacle. After suitable incubation, the second cell population forms a layer on the second surface of the membrane.
5583042 A P P A R A T U S FOR THE SYNTHESIS OF S A C C H A R I D E COMPOSITIONS Roth Stephe Gladwyne, PA, UNITED STATES Assigned to NEOSE Pharmaceuticals Inc This invention relates to an apparatus containing specific binary combinations of glycosyltransferases, for the synthesis of specific saccharide compositions such as, for example, oligosaccharides, polysaccharides, glycolipids, and glycopeptides.
5583043 C O V E R SLIP H O L D E R FOR BILATERAL A N D U N I L A T E R A L S I M U L A T I O N OF PARTLY T H R O M B O G E N E S I S IN O C C L U D E D B L O O D VESSELS AND USE T H E R E O F Sakariassen Kjell S Oslo, NORWAY Assigned to Hafslund Nycomed As