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5585103 Induction of cytotoxic T-lymphocyte responses
Agriculture and Forestry Biotechnology 5580852
5585098
DERIVATIVES OF TACHYPLESIN HAVING INHIBITORY ACTIVITY TOWARDS PLANT PATHOGENIC FUNGI
ORAL ADMINISTRATION OF CHICKEN YOLK IMMUNOGLOBULINS TO LOWER SOMATIC CELL COUNT IN THE MILK OF LACTATING RUMINANTS
Putnam Rebecca J; Rao A Gururaj West Des Moines, IA, UNITED STATES Assigned to Pioneer Hi-Bred International lnc
Coleman Marilyn A Columbus, OH, UNITED STATES Assigned to Ovimmune Inc A method for lowering somatic cell count in the milk of a lactating ruminant is disclosed. IgY antibodies are fn-st obtained from the egg of a hen which has been actively immunized against one or more mastitis-causing pathogenic organisms by injection with an immunogen containing immunogenic determinants specific to elicit such antibodies. The immunogenic determinant may comprise only a specific portion of the pathogenic organism, e.g., the fimbria of a piliated bacterium. The IgY antibodies are then administered orally to a ruminant in which it is desired to lower milk somatic cell count. Antibody administration may occur during a ruminant's dry period as well as during lactation. In a preferred embodiment, the antigen used in immunization of the hen comprises one or more of Staphylococcus anreus and Streptococcus agalactiae. The method of this invention has been shown to be efficacious in lowering somatic cell count in dairy cattle.
Certain specific derivatives of the 17-amino acid peptide tachyplesin has been found to have antimicrobial activity against plant pathogens. In a preferred embodiment, plant resistance to diseases caused by plant pathogens which are susceptible to these peptides is produced by inserting into the cells of a plant a gene whose expression causes production of one or more of the peptides of this invention in the plant in antimicrobial amounts.
5583036
REGENERATION OF COTTON PLANT IN SUSPENSION CULTURE
5585103
INDUCTION OF CYTOTOXIC T-LYMPHOCYTE RESPONSES
Rangan Thirumale S; Rajasekaran Kanniah San Dimas, CA, UNITED STATES Assigned to Phytogen
Raychaudhuri Syamal; Rastetter William H San Diego, CA, UNITED STATES Assigned to IDEC Pharmaceutical Corporation
A method for the regeneration of a cotton plant from somatic cells. The method comprises providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until the secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant and culturing the undifferentiated callus in callus growth medium supplemented with sucrose as a primary carbon source until embryogenic callus is formed from the callus.
Methods and compositions useful for inducing a cytotoxic T lymphocyte response (CTL) in a human or domesticated or agriculturally important animal. The method includes the steps of providing the antigen to which the CTL response is desired and providing an antigen formulation which comprises, consists, or consists essentially of two or more of a stabilizing detergent, a 791
792
PATENT ABSTRACTS
micelle-forming agent, and an oil. This antigen formulation is preferably lacking in an immunostimulating peptide component, or has sufficiently low levels of such a component that the desired CTL response is not diminished. This formulation is provided as a stable oil-in-water emulsion.
5585544 M E T H O D OF CAUSING SOMATIC HYBRIDIZATION B E T W E E N T W O SPECIES OF ALGAE Cheney Donald P; Wang Le Zhong Ipswich, MA, UNITED STATES Assigned to Northeastern University A method of causing somatic cell hybridization between two species of algae by growing somatic algal tissue from each of the two species of algae in close proximity, in a nutrient solution, and isolating and culturing the hybrid somatic shoots that form.
5591616 METHOD FOR TRANSFORMING MONOCOTYLEDONS Hiei Yokoh; Komari Toshihiko lwata gun, JAPAN Assigned to Japan Tobacco Inc PCT No. PCT/JP93/00925 Sec. 371 Date May 3, 1994 Sec. 102(e) Date May 3, 1994 PCT Filed Jul. 6, 1993 PCT Pub. No. WO94/00977 PCT Pub. Date Jan. 20, 1994. A method for transforming a monocotyledon by which the time required from transformation to regeneration of a plant is shorter so that the frequency of emergence of mutants is smaller than the conventional methods, which may be generally applied even to the plants for which the regeneration method from a protoplast to a plant has not been established, and with which the preparation of the material to be subjected to the method is easy. That is, the present invention provides a method for transforming a monocotyledon comprising transforming a cultured tissue during de,differentiation process or a dedifferentiated cultured tissue of said monocotyledon with a bacterium belonging to
genus Agrobacterium containing a desired gene.
5595896 EXPRESSION OF H E T E R O L O G O U S GENES IN TRANSGENIC PLANTS A N D PLANT CELLS USING PLANT ASPARAGINE S Y N T H E T A S E PROMOTERS Comzzi Gloria; Tsai Fong-Ying New York, NY, UNITED STATES Assigned to New York University The identification and cloning of the gene(s) for plant asparagine synthetase Assigned to, an important enzyme involved in the formation of asparagine, a major nitrogen transport compound of higher plants is described. Expression vectors constructed with the AS coding sequence may be utilized to produce plant AS; to engineer herbicide resistant plants, salt/drought tolerant plants or pathogen resistant plants; as a dominant selectable marker; or to select for novel herbicides or compounds useful as agents that synchronize plant cells in culture. The promoter for plant AS, which directs high levels of gene expression and is induced in an organ specific manner and by darkness, is also described. The AS promoter may be used to direct the expression of heterologous coding sequences in appropriate hosts.
5596131 R E G E N E R A T I O N OF G R A M I N A C E O U S PLANTS OF THE SUBFAMILY P O O I D E A E F R O M PROTOPLASTS Horn Michael E; Harms Christian T; Shillito Raymond Woodland, CA, UNITED STATES Assigned to Ciba-Geigy Corporation The present invention provides cell cultures derived from graminaceous plants which cultures are capable of being regenerated into plants, including fertile plants. Methods of accomplishing this regeneration are also provided. In addition, a novel procedure for the cryopreservation of embryogenic cell cultures is described.
5585098
DERIVATIVES OF TACHYPLESIN HAVING INHIBITORY ACTIVITY TOWARDS PLANT PATHOGENIC FUNGI
ORAL ADMINISTRATION OF CHICKEN YOLK IMMUNOGLOBULINS TO LOWER SOMATIC CELL COUNT IN THE MILK OF LACTATING RUMINANTS
Putnam Rebecca J; Rao A Gururaj West Des Moines, IA, UNITED STATES Assigned to Pioneer Hi-Bred International lnc
Coleman Marilyn A Columbus, OH, UNITED STATES Assigned to Ovimmune Inc A method for lowering somatic cell count in the milk of a lactating ruminant is disclosed. IgY antibodies are fn-st obtained from the egg of a hen which has been actively immunized against one or more mastitis-causing pathogenic organisms by injection with an immunogen containing immunogenic determinants specific to elicit such antibodies. The immunogenic determinant may comprise only a specific portion of the pathogenic organism, e.g., the fimbria of a piliated bacterium. The IgY antibodies are then administered orally to a ruminant in which it is desired to lower milk somatic cell count. Antibody administration may occur during a ruminant's dry period as well as during lactation. In a preferred embodiment, the antigen used in immunization of the hen comprises one or more of Staphylococcus anreus and Streptococcus agalactiae. The method of this invention has been shown to be efficacious in lowering somatic cell count in dairy cattle.
Certain specific derivatives of the 17-amino acid peptide tachyplesin has been found to have antimicrobial activity against plant pathogens. In a preferred embodiment, plant resistance to diseases caused by plant pathogens which are susceptible to these peptides is produced by inserting into the cells of a plant a gene whose expression causes production of one or more of the peptides of this invention in the plant in antimicrobial amounts.
5583036
REGENERATION OF COTTON PLANT IN SUSPENSION CULTURE
5585103
INDUCTION OF CYTOTOXIC T-LYMPHOCYTE RESPONSES
Rangan Thirumale S; Rajasekaran Kanniah San Dimas, CA, UNITED STATES Assigned to Phytogen
Raychaudhuri Syamal; Rastetter William H San Diego, CA, UNITED STATES Assigned to IDEC Pharmaceutical Corporation
A method for the regeneration of a cotton plant from somatic cells. The method comprises providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until the secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant and culturing the undifferentiated callus in callus growth medium supplemented with sucrose as a primary carbon source until embryogenic callus is formed from the callus.
Methods and compositions useful for inducing a cytotoxic T lymphocyte response (CTL) in a human or domesticated or agriculturally important animal. The method includes the steps of providing the antigen to which the CTL response is desired and providing an antigen formulation which comprises, consists, or consists essentially of two or more of a stabilizing detergent, a 791
792
PATENT ABSTRACTS
micelle-forming agent, and an oil. This antigen formulation is preferably lacking in an immunostimulating peptide component, or has sufficiently low levels of such a component that the desired CTL response is not diminished. This formulation is provided as a stable oil-in-water emulsion.
5585544 M E T H O D OF CAUSING SOMATIC HYBRIDIZATION B E T W E E N T W O SPECIES OF ALGAE Cheney Donald P; Wang Le Zhong Ipswich, MA, UNITED STATES Assigned to Northeastern University A method of causing somatic cell hybridization between two species of algae by growing somatic algal tissue from each of the two species of algae in close proximity, in a nutrient solution, and isolating and culturing the hybrid somatic shoots that form.
5591616 METHOD FOR TRANSFORMING MONOCOTYLEDONS Hiei Yokoh; Komari Toshihiko lwata gun, JAPAN Assigned to Japan Tobacco Inc PCT No. PCT/JP93/00925 Sec. 371 Date May 3, 1994 Sec. 102(e) Date May 3, 1994 PCT Filed Jul. 6, 1993 PCT Pub. No. WO94/00977 PCT Pub. Date Jan. 20, 1994. A method for transforming a monocotyledon by which the time required from transformation to regeneration of a plant is shorter so that the frequency of emergence of mutants is smaller than the conventional methods, which may be generally applied even to the plants for which the regeneration method from a protoplast to a plant has not been established, and with which the preparation of the material to be subjected to the method is easy. That is, the present invention provides a method for transforming a monocotyledon comprising transforming a cultured tissue during de,differentiation process or a dedifferentiated cultured tissue of said monocotyledon with a bacterium belonging to
genus Agrobacterium containing a desired gene.
5595896 EXPRESSION OF H E T E R O L O G O U S GENES IN TRANSGENIC PLANTS A N D PLANT CELLS USING PLANT ASPARAGINE S Y N T H E T A S E PROMOTERS Comzzi Gloria; Tsai Fong-Ying New York, NY, UNITED STATES Assigned to New York University The identification and cloning of the gene(s) for plant asparagine synthetase Assigned to, an important enzyme involved in the formation of asparagine, a major nitrogen transport compound of higher plants is described. Expression vectors constructed with the AS coding sequence may be utilized to produce plant AS; to engineer herbicide resistant plants, salt/drought tolerant plants or pathogen resistant plants; as a dominant selectable marker; or to select for novel herbicides or compounds useful as agents that synchronize plant cells in culture. The promoter for plant AS, which directs high levels of gene expression and is induced in an organ specific manner and by darkness, is also described. The AS promoter may be used to direct the expression of heterologous coding sequences in appropriate hosts.
5596131 R E G E N E R A T I O N OF G R A M I N A C E O U S PLANTS OF THE SUBFAMILY P O O I D E A E F R O M PROTOPLASTS Horn Michael E; Harms Christian T; Shillito Raymond Woodland, CA, UNITED STATES Assigned to Ciba-Geigy Corporation The present invention provides cell cultures derived from graminaceous plants which cultures are capable of being regenerated into plants, including fertile plants. Methods of accomplishing this regeneration are also provided. In addition, a novel procedure for the cryopreservation of embryogenic cell cultures is described.