5587288 Regulation of exoprotein in staphylococcus aureus

704

PATENT ABSTRACTS

without need for isolation of nucleic acid from the cells. After the amplification, an assay can be carried out for amplified nucleic acid segment indicative of the presence of cells of interest in the sample. The invention thus provides methods for obtaining cellular components from samples of milk, and cultures or extracts of other materials, including food materials, and for determining relative contamination of milk and such other materials by microorganisms. The invention also provides kits for carrying out its various methods.

control of bacterial infections, particularly infections caused by Staphylococcus aureus (S. aureus). The present invention provides the staphylococcal accessory regulatory (sar) protein, and the gene encoding the sar protein (sar). The present invention also provides a diagnostic method for determining the pathogenicity of an S. aureus isolate using the sar gene.

5587289 ISOLATED NUCLEIC ACID MOLECULES WHICH ARE

5587287

MEMBERS THERMOSTABLE POLYMERASE

FAMILY

OF THE

MAGE-XP

AND USES THEREOF

SPECIFIC

ANTIBODY-CONTAINING AMPLIFICATION COMPOSITION

DNA

Lurquin Christoph; Boon-Falleur Thierry Brussels, BELGIUM Assigned to Ludwig Institute for Cancer Research

AND KIT

Scalice Edward; Sharkey David; Christy Kenneth G; Esders Theodore W; Daiss John L Rochester, NY, UNITED STATES Assigned to Johnson & Johnson Clinical Diagnostics Inc Antibodies which are specific to a thermostable DNA polymerase can be used to reduce or eliminate the formation of non-specific products in polymerase chain reaction methods. These antibodies and other temperature sensitive inhibitors are effective to inhibit DNA polymerase enzymatic activity at a certain temperature TI which is generally below about 85°C. The inhibitors are irreversibly inactivated at temperature T2 which is generally above about 40°C T2 is also greater than TI. Such inhibitors can be supplied individually or in admixture with the DNA polymerase in a diagnostic test kit suitable for PCR.

The invention relates to members of the MAGE-Xp family of nucleic acid molecules. These molecules differ from the previously described MAGE nucleic acid molecules in that members of the MAGE-Xp family do not hybridize to the previously identified MAGE sequences. Further, the members of the MAGE-Xp family are found on the Xp arm of the X chromosome rather than on the Xq chromosome, as was the case with the previously identified MAGE genes.

5587290 STRESS

TOLERANT

YEAST

MUTANTS Klionsky Daniel; Holzer Helmut; Destruelle Monika Davis, CA, UNITED STATES Assigned to The Regents of the University of California

5587288 REGULATION EXOPROTEIN STAPHYLOCOCCUS

OF IN AUREUS

Cheung Ambros; Fischetti Vincent A New York, NY, UNITED STATES Assigned to The Rockefeller University This invention relates to the recognition and

The invention provides methods and compositions relating to stress tolerant yeast; in particular, yeast mutants deficient in the expression of functional ATH1 gene product (Athlp). Such yeast have enhanced tolerance to dehydration and freezing, are able to grow to a higher cell density over a range of fermentable carbon source concentrations, are able to produce and/or tolerate higher levels of ethanol, and trehalose. Nucleic acids comprising ATH1 gene sequences are used in hybridization probes and PCR primers, in