- Email: [email protected]
5589623 Genetic control of ethylene biosynthesis in plants
664
PATENT ABSTRACTS
sequence beginning at nucleotide 438 and ending at nucleotide 751 of said gene coding for said antigen of all species of mycobacteria except for the species M. tuberculosis, M. bovis BCG, M. avium, M. paratuberculosis, M. fortuitum, M. malmoense, M. leprae, M. kansaii and M. marinurn.
DNA molecules useful for producing plant cells containing monocot seed storage proteins are also disclosed. The invention is exemplified by combination of a 15 kD zein structural gene from a Zea mays gene with a promoter and a polyadenylation site derived from a Phaseolus vulgaris phaseolin gene.
5589615
5589622
P R O C E S S FOR THE P R O D U C T I O N OF T R A N S G E N I C PLANTS W I T H INCREASED NUTRITIONAL V A L U E VIA THE EXPRESSION OF M O D I F I E D 2S S T O R A G E ALBUMINS
PLANT PARASITIC N E M A T O D E CONTROL
De Ciercq Ann; Krebbers Enno; Vandekerckhove Joacu el; Barreto De Castro Lui; Gander Eugen; Van Montagn Mar Harelbeke, BELGIUM Assigned to Plant Genetic Systems N V; Brazilian Agricultural Research Organization-Embrapa/Cenarg A process for the production of plants with increased content of appropriate amino acids having high nutritional properties through the modification of plant genes encoding plant storage proteins, particularly the 2S albumins. Seed-fonmng plants susceptible to Agrobacterium transformation, the genome of which plants includes a recombinant DNA encoding a precursor of a modified 2S albumin, under the control of a promoter, are described, including the particular recombinant DNA.
5589616 M O N O C O T SEED S T O R A G E P R O T E I N S IN DICOTS Hoffman Leslie M Madison, UNITED STATES Assigned to Mycogen Plant Science Inc The present invention discloses dicot cells containing monocot seed storage protein. Construction of genes encoding monocot seed storage proteins which are expressible in dicot cells and transformation of such genes into plant cells is also taught. Furthermore, methods and
Gurr Sarah J; McPherson Michael J; Atkinson Howard; Bowles Dianna J York, UNITED KINGDOM Assigned to Advanced Technologies (Cambridge) Ltd A method of controlling nematodes, the method includes the steps of identification of a gene induced within a successfully infected plant by nematode infection of said plant and modifying the gene to confer nematode resistance to the plant.
5589623 GENETIC C O N T R O L OF E T H Y L E N E BIOSYNTHESIS IN PLANTS Ferro Adolph J; Bestwick Richard K; Brown Lyle R Lake Oswego, OR, UNITED STATES Assigned to Agritope Inc A method for control of ethylene biosynthesis in plants comprising a vector containing a selective gene under plant promoter control, and a DNA insert comprising codons for a functional heterologous polypeptide having AdoMetase activity and flanked by a plant promoter on one side and a polyA signal sequence on the other side; and, transforming a plant host with said vector wherein the plant host transformed thereby is capable of expressing the heterologous polypeptide having AdoMetase activity under the control of said control region. The presence of the AdoMetase gene and the expression of AdoMetase in transgenic plants lowers AdoMet levels and generates an inhibitor of ACC synthase causing a corresponding decrease in ethylene biosynthesis and precursor availability. The
PATENT ABSTRACTS
current construction of transgenic plants containing a copy(s) of the T3 AdoMetase gene allow for construction of plants that will control ethylene biosynthesis under restricted conditions resulting in fruits, vegetables, and flowers which have been modified internally to improve shelf life and preservation qualities.
5589625 T R A N S G E N I C PLANTS D I S P L A Y I N G M U L T I P L E VIRUS R E S I S T A N C E A N D A PROCESS FOR THEIR PRODUCTION Saanna Mart; Kelve Merikke; Truve Erkki; Teed Teemu Helsinki, FINLAND Assigned to Kemira Oy Biotech This invention discloses transgenic plants, such as transgenic tobacco and potato, having resistance to multiple viral taxonomic groups using parts of the 2,5A oligoadenylate pathway. In particular, said plants are genetically engineered to contain a DNA sequence encoding at least one polypeptide having a 2,5A synthetase activity. By this means a step in the 2,5A oligoadenylate pathway heretofore believed to be missing in all plants is provided so that viral infection in the transgenic plants is inhibited via a 2,5A dependent endonuclease. Moreover, this invention relates to a process for the production of said transgenic plants by transfection with a genetically engineered DNA sequence encoding at least one polypeptide having a 2,5A synthetase activity.
665
signal peptide sequence of barley alpha-amylase and one or more lysozyme genes.
5591579 I N D I C A T O R CELL LINE FOR D E T E C T I N G RNA VIRUSES AND METHOD THEREFOR Olivo Paul; Schlesinger Sondra St Louis, MO, UNITED STATES Assigned to Washington University Cell lines and methods are disclosed for detecting the presence of RNA viruses in a specimen. The cell lines are stably transformed with a DNA molecule that includes a promoter capable of being recognized by the DNA dependent RNA polymerase of the cell capable of directing the transcription of a cDNA of a structurally defective RNA virus genome operably coupled to the promoter. The cDNA contains a structural coding sequence encoding a selected reporter gene product. The RNA molecules transcribed by the DNA dependent RNA polymerase are not capable of causing the translation of the reporter gene in the cell except when an active related virus that provides the necessary trans-acting enzymes to cause the increased replication of the RNA containing the reporter gene which is then translated into the reporter gene product is provided. Methods utilizing the cell lines of this invention to detect RNA viruses in a specimen by incubating the specimen with the cell line and assaying for expression of the reporter gene and a kit containing a supply of the ceils and a supply of the reagents necessary for the detection of the reporter gene product are also provided.
5589626 USE OF L Y S O Z Y M E GENE S T R U C T U R E S IN PLANTS TO INCREASE RESISTANCE Hain Ruml udiger; Stenzel Klaus Langenfeld, GERMANY Assigned to Bayer Aktiengesellschaft A method for increasing the resistance of a plant to fungi and animal pests comprising introducing into the genome of the plant one or more lysozyme gene structures which express lysozyme, the lysozyme gene structure comprises a chimeric gene fusion of the TR promoter, the
5591601 DNA P O L Y M E R A S E GENE EXPRESSION SYSTEM UTILIZING AN RNA POLYMERASE CO-DELIVERED WITH THE GENE EXPRESSION V E C T O R SYSTEM Wagner Thomas; Chen Xiaozhuo; Li Yunsheng Albany, OH, UNITED STATES Assigned to Ohio University Edison Animal Biotechnology Institute
PATENT ABSTRACTS
sequence beginning at nucleotide 438 and ending at nucleotide 751 of said gene coding for said antigen of all species of mycobacteria except for the species M. tuberculosis, M. bovis BCG, M. avium, M. paratuberculosis, M. fortuitum, M. malmoense, M. leprae, M. kansaii and M. marinurn.
DNA molecules useful for producing plant cells containing monocot seed storage proteins are also disclosed. The invention is exemplified by combination of a 15 kD zein structural gene from a Zea mays gene with a promoter and a polyadenylation site derived from a Phaseolus vulgaris phaseolin gene.
5589615
5589622
P R O C E S S FOR THE P R O D U C T I O N OF T R A N S G E N I C PLANTS W I T H INCREASED NUTRITIONAL V A L U E VIA THE EXPRESSION OF M O D I F I E D 2S S T O R A G E ALBUMINS
PLANT PARASITIC N E M A T O D E CONTROL
De Ciercq Ann; Krebbers Enno; Vandekerckhove Joacu el; Barreto De Castro Lui; Gander Eugen; Van Montagn Mar Harelbeke, BELGIUM Assigned to Plant Genetic Systems N V; Brazilian Agricultural Research Organization-Embrapa/Cenarg A process for the production of plants with increased content of appropriate amino acids having high nutritional properties through the modification of plant genes encoding plant storage proteins, particularly the 2S albumins. Seed-fonmng plants susceptible to Agrobacterium transformation, the genome of which plants includes a recombinant DNA encoding a precursor of a modified 2S albumin, under the control of a promoter, are described, including the particular recombinant DNA.
5589616 M O N O C O T SEED S T O R A G E P R O T E I N S IN DICOTS Hoffman Leslie M Madison, UNITED STATES Assigned to Mycogen Plant Science Inc The present invention discloses dicot cells containing monocot seed storage protein. Construction of genes encoding monocot seed storage proteins which are expressible in dicot cells and transformation of such genes into plant cells is also taught. Furthermore, methods and
Gurr Sarah J; McPherson Michael J; Atkinson Howard; Bowles Dianna J York, UNITED KINGDOM Assigned to Advanced Technologies (Cambridge) Ltd A method of controlling nematodes, the method includes the steps of identification of a gene induced within a successfully infected plant by nematode infection of said plant and modifying the gene to confer nematode resistance to the plant.
5589623 GENETIC C O N T R O L OF E T H Y L E N E BIOSYNTHESIS IN PLANTS Ferro Adolph J; Bestwick Richard K; Brown Lyle R Lake Oswego, OR, UNITED STATES Assigned to Agritope Inc A method for control of ethylene biosynthesis in plants comprising a vector containing a selective gene under plant promoter control, and a DNA insert comprising codons for a functional heterologous polypeptide having AdoMetase activity and flanked by a plant promoter on one side and a polyA signal sequence on the other side; and, transforming a plant host with said vector wherein the plant host transformed thereby is capable of expressing the heterologous polypeptide having AdoMetase activity under the control of said control region. The presence of the AdoMetase gene and the expression of AdoMetase in transgenic plants lowers AdoMet levels and generates an inhibitor of ACC synthase causing a corresponding decrease in ethylene biosynthesis and precursor availability. The
PATENT ABSTRACTS
current construction of transgenic plants containing a copy(s) of the T3 AdoMetase gene allow for construction of plants that will control ethylene biosynthesis under restricted conditions resulting in fruits, vegetables, and flowers which have been modified internally to improve shelf life and preservation qualities.
5589625 T R A N S G E N I C PLANTS D I S P L A Y I N G M U L T I P L E VIRUS R E S I S T A N C E A N D A PROCESS FOR THEIR PRODUCTION Saanna Mart; Kelve Merikke; Truve Erkki; Teed Teemu Helsinki, FINLAND Assigned to Kemira Oy Biotech This invention discloses transgenic plants, such as transgenic tobacco and potato, having resistance to multiple viral taxonomic groups using parts of the 2,5A oligoadenylate pathway. In particular, said plants are genetically engineered to contain a DNA sequence encoding at least one polypeptide having a 2,5A synthetase activity. By this means a step in the 2,5A oligoadenylate pathway heretofore believed to be missing in all plants is provided so that viral infection in the transgenic plants is inhibited via a 2,5A dependent endonuclease. Moreover, this invention relates to a process for the production of said transgenic plants by transfection with a genetically engineered DNA sequence encoding at least one polypeptide having a 2,5A synthetase activity.
665
signal peptide sequence of barley alpha-amylase and one or more lysozyme genes.
5591579 I N D I C A T O R CELL LINE FOR D E T E C T I N G RNA VIRUSES AND METHOD THEREFOR Olivo Paul; Schlesinger Sondra St Louis, MO, UNITED STATES Assigned to Washington University Cell lines and methods are disclosed for detecting the presence of RNA viruses in a specimen. The cell lines are stably transformed with a DNA molecule that includes a promoter capable of being recognized by the DNA dependent RNA polymerase of the cell capable of directing the transcription of a cDNA of a structurally defective RNA virus genome operably coupled to the promoter. The cDNA contains a structural coding sequence encoding a selected reporter gene product. The RNA molecules transcribed by the DNA dependent RNA polymerase are not capable of causing the translation of the reporter gene in the cell except when an active related virus that provides the necessary trans-acting enzymes to cause the increased replication of the RNA containing the reporter gene which is then translated into the reporter gene product is provided. Methods utilizing the cell lines of this invention to detect RNA viruses in a specimen by incubating the specimen with the cell line and assaying for expression of the reporter gene and a kit containing a supply of the ceils and a supply of the reagents necessary for the detection of the reporter gene product are also provided.
5589626 USE OF L Y S O Z Y M E GENE S T R U C T U R E S IN PLANTS TO INCREASE RESISTANCE Hain Ruml udiger; Stenzel Klaus Langenfeld, GERMANY Assigned to Bayer Aktiengesellschaft A method for increasing the resistance of a plant to fungi and animal pests comprising introducing into the genome of the plant one or more lysozyme gene structures which express lysozyme, the lysozyme gene structure comprises a chimeric gene fusion of the TR promoter, the
5591601 DNA P O L Y M E R A S E GENE EXPRESSION SYSTEM UTILIZING AN RNA POLYMERASE CO-DELIVERED WITH THE GENE EXPRESSION V E C T O R SYSTEM Wagner Thomas; Chen Xiaozhuo; Li Yunsheng Albany, OH, UNITED STATES Assigned to Ohio University Edison Animal Biotechnology Institute