5594123 Primers and probes for the amplification and detection of AIDS associated nucleic acids

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PATENT ABSTRACTS

5594121

5594123

E N H A N C E D TRIPLE-HELIX AND DOUBLE-HELIX F O R M A T I O N WITH O L I G O M E R S CONTAINING M O D I F I E D PURINES

PRIMERS AND PROBES FOR THE AMPLIFICATION A N D DETECTION OF AIDS ASSOCIATED NUCLEIC ACIDS

Froehler Brian; Matteucci Mark Belmont, CA, UNITED STATES Assigned to Gilead Sciences lnc Novel oligomers are disclosed which have enhanced ability with respect to forming duplexes or triplexes compared with oligomers containing only conventional bases. The oligomers contain 7-deaza-7-substituted purines or related analogs. The oligomers of the invention are capable of (i) forming triplexes with various target sequences such as virus or oncogene sequences by coupling into the major groove of a target DNA duplex at physiological pH or (ii) forming duplexes by binding to single-stranded DNA or to RNA encoded by target genes. The oligomers of the invention can be constructed to have any desired sequence, provided the sequence normally includes one or more bases that is replaced with the analogs of the invention. Compositions of the invention can be used for diagnostic purposes in order to detect viruses or disease conditions.

Sninsky John; Kwok Shirley El Sobrante, CA, UNITED STATES Assigned to Hoffman-La Roche lnc The presence or absence of a nucleic acid sequence associated with AIDS in a sample containing one or more nucleic acids and suspected of containing such sequence can be detected by amplifying the sequence using primers to form extension products as templates and detecting the amplified product if it is present. This may be accomplished by adding a labeled hybridization probe to the amplified product either free in solution or after immobilization on a solid support.

5595650 DEVICE AND A M E T H O D FOR THE SEPARATION OF FLUID SUBSTANCES Manz Andreas Bettingen, SWITZERLAND Assigned to Ciba-CJeigy Corporation

5594122 ANTISENSE OLIGONUCLEOTIDES T A R G E T E D AGAINST H U M A N I M M U N O D E F I C I E N C Y VIRUS Friesen Albert D Winnipeg, CANADA Assigned to Genesys Pharma Inc The present invention is directed to oligonucleoddes comprising nucleotide sequences sufficiently complementary to conserved regions of human immunodeficioncy virus genetic material such that when bound to said region, the oligonucleotides effectively prevent expression of the genetic material.

A device for the separation of fluid substances, especially a capillary chromatographic separating device, is provided, which comprises a separating path in which a stationary phase is arranged and through which flows a mobile phase containing a sample to be separated into its components. The device also comprises means for transporting the mobile phase, and a detection device for detecting the separated components. The separating path is constructed in the form of an annular channel into which lead the inflow and outflow channels for the mobile phase and the fluid substance to be separated. In a very especially prefe.a,red variant of the invention the means for transporting the mobile phase are arranged inside the annular channel. In the described method of separating fluid substances the mobile phase together with the sample to be separated is circulated cyclically in a separating path constructed in the form of an annular channel. The circulation is preferably effected with the aid of transport means arranged