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5599702 D-amino acid oxidase from trigonopsis variabilis immobilized on porous copolymer beads
PATENT ABSTRACTS
linkers with known nucleotide sequences are used in the method to isolate and distinguish the specific undefined nucleic acid fragments from the rest of the nucleic acid. Primers which hybridize with the known linker are then used as part of a polymerase chain reaction procedure to amplify the specific nucleic acid fragment which includes the undefined nucleotide sequence.
5599702 D - A M I N O ACID OXIDASE F R O M TRIGONOPSIS VARIABILIS I M M O B I L I Z E D ON P O R O U S C O P O L Y M E R BEADS Sauber Klan Bad Soden am Taunus, GERMANY Assigned to Hoechst Aktiengesellschaft D-amino acid oxidase (DAO) in purified, partially purified or crude form is immobilized on a porous bead-shaped support that is a copolymer of vinyl acetate and/or vinyl alcohol and a crosslinking agent. A preferred crosslinking agent is N,N'-divinylethyleneurea. The amount of crosslinking agent is 1 to 60% by weight of the copolymer, and acyloxy groups of vinyl acetate may be present on the support as such or may have been hydrolyzed to hydroxyl groups. The support preferably has a mean particle size of 20 to 800 m u m and a mean pore diameter of 2 to 10,000 nm. The D-amino acid oxidase is preferably obtained from Trigonopsis variabilis and purified by ion exchange chromatography. The D-amino acid oxidase may be covalently bonded to a spacer such as epichlorohydrin or its homolog that is bound to the support. Catalase may also be immobilized.
727
5599705 IN VITRO M E T H O D FOR PRODUCING DIFFERENTIATED UNIVERSALLY COMPATIBLE MATURE HUMAN BLOOD CELLS Cameron Robert B Agoura Hill, CA, UNITED STATES In vitro production of clinically useful quantities of mature, differentiated human blood cells by a method in which human pluripotent hematopoietic stem cells, preferably from a universal donor, are incubated in a bioreactor in a growth medium also containing specific recombinant human growth and maturation promoting polypeptide factors in combinations that expand stem cell cultures and promote the maturation and differention of stem cells into erythroid, thrombocytic or granulocytic human blood cells, and harvesting the mature cells. The growth and maturation promoting polypeptides employed include SCGF, Interleukins 1,3,4,5,6, and 11, GM-CSF, M-CSF, G-CSF and EPO. Stem cells may be modified so as to remove histocompatibility and/or blood group antigens, or may be genetically altered by transfection with appropriate DNA-contalning vectors, prior to addition to the bioreactor. Erythrocytes prepared in large quantities by this method are a good source of iron, as iron-saturated hemoglobin, for use in iron replacement therapy.
5599704 5599706 ERBB2/NEU TARGETED RIBOZYMES
RIBOZYMES TARGETED TO APO(A)
Thompson James D; Draper Kenneth G Boulder, CO, UNITED STATES Assigned to Ribozyme Pharmaceuticals Inc An enzymatic RNA molecule which cleaves mRNA associated with development or maintenance of breast cancer.
MRNA
Stinchcomb Dan T; McSwiggen Jame;Newton Roger; Ramharack Rand Boulder, CO, 80301, UNITED STATES Enzymatic RNA molecules which cleave apo(a) mRNA.
linkers with known nucleotide sequences are used in the method to isolate and distinguish the specific undefined nucleic acid fragments from the rest of the nucleic acid. Primers which hybridize with the known linker are then used as part of a polymerase chain reaction procedure to amplify the specific nucleic acid fragment which includes the undefined nucleotide sequence.
5599702 D - A M I N O ACID OXIDASE F R O M TRIGONOPSIS VARIABILIS I M M O B I L I Z E D ON P O R O U S C O P O L Y M E R BEADS Sauber Klan Bad Soden am Taunus, GERMANY Assigned to Hoechst Aktiengesellschaft D-amino acid oxidase (DAO) in purified, partially purified or crude form is immobilized on a porous bead-shaped support that is a copolymer of vinyl acetate and/or vinyl alcohol and a crosslinking agent. A preferred crosslinking agent is N,N'-divinylethyleneurea. The amount of crosslinking agent is 1 to 60% by weight of the copolymer, and acyloxy groups of vinyl acetate may be present on the support as such or may have been hydrolyzed to hydroxyl groups. The support preferably has a mean particle size of 20 to 800 m u m and a mean pore diameter of 2 to 10,000 nm. The D-amino acid oxidase is preferably obtained from Trigonopsis variabilis and purified by ion exchange chromatography. The D-amino acid oxidase may be covalently bonded to a spacer such as epichlorohydrin or its homolog that is bound to the support. Catalase may also be immobilized.
727
5599705 IN VITRO M E T H O D FOR PRODUCING DIFFERENTIATED UNIVERSALLY COMPATIBLE MATURE HUMAN BLOOD CELLS Cameron Robert B Agoura Hill, CA, UNITED STATES In vitro production of clinically useful quantities of mature, differentiated human blood cells by a method in which human pluripotent hematopoietic stem cells, preferably from a universal donor, are incubated in a bioreactor in a growth medium also containing specific recombinant human growth and maturation promoting polypeptide factors in combinations that expand stem cell cultures and promote the maturation and differention of stem cells into erythroid, thrombocytic or granulocytic human blood cells, and harvesting the mature cells. The growth and maturation promoting polypeptides employed include SCGF, Interleukins 1,3,4,5,6, and 11, GM-CSF, M-CSF, G-CSF and EPO. Stem cells may be modified so as to remove histocompatibility and/or blood group antigens, or may be genetically altered by transfection with appropriate DNA-contalning vectors, prior to addition to the bioreactor. Erythrocytes prepared in large quantities by this method are a good source of iron, as iron-saturated hemoglobin, for use in iron replacement therapy.
5599704 5599706 ERBB2/NEU TARGETED RIBOZYMES
RIBOZYMES TARGETED TO APO(A)
Thompson James D; Draper Kenneth G Boulder, CO, UNITED STATES Assigned to Ribozyme Pharmaceuticals Inc An enzymatic RNA molecule which cleaves mRNA associated with development or maintenance of breast cancer.
MRNA
Stinchcomb Dan T; McSwiggen Jame;Newton Roger; Ramharack Rand Boulder, CO, 80301, UNITED STATES Enzymatic RNA molecules which cleave apo(a) mRNA.