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5604096 Fluorometric quantitation of mycoplasmas
PATENT ABSTRACTS
The bis-naphthalimides provided herein have exceptional DNA binding properties and demonstrate cytotoxicity in both in vitro and in vivo tumor models, in particular, against melanoma.
5604096 FLUOROMETRIC Q U A N T I T A T I O N OF MYCOPLASMAS Schaeffer Warren; Melamede Rober Burlington, VT, UNITED STATES The present invention pertains to methods of monitoring mycoplasmal growth whereby a culture of mycoplasmal cells is contacted with a fluorescent nucleic acid-binding agent under conditions which allow the agent to selectively bind to mycoplasmal nucleic acid. The amount of fluorescence associated with the mycoplasmal nucleic acid indicates the number of mycoplasmal cells in the culture. The fluorescence of the culture is typically read using a fluorometer which correlates with colonial growth on agar. A particularly preferred fluorescent nucleic acid-binding agent is ethidium bromide. The present invention further pertains to methods of determining the effectiveness of an antimycoplasmal antibiotic.
5604097 METHODS FOR SORTING P O L Y N U C L E O T I D E S USING O L I G O N U C L E O T I D E TAGS Brenner Sydney Cambridge, UNITED KINGDOM Assigned to Spectragen Inc The invention provides a method of tracking, identifying, and/or sorting classes or subpopulations of molecules by the use of oligonucleotide tags. Oligonucleotide tags of the invention each consist of a plurality of subunits 3 to 6 nucleotides in length selected from a minimally cross-hybridizing set. A subunit of a minimally cross-hybridizing set forms a duplex or triplex having two or more mismatches with the complement of any other subunit of the same set. The number of oligonucleotide tags available in a
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particular embodiment depends on the number of subunits per tag and on the length of the subunit. An important aspect of the invention is the use of the oligonucleotide tags for sorting polynucleotides by specifically hybridizing tags attached to the polynucleotides to their complements on solid phase supports. This embodiment provides a readily automated system for manipulating and sorting polynucleotides, particularly useful in large-scale parallel operations, such as large-scale DNA sequencing, mRNA fingerprinting, and the like, wherein many target polynucleotides or many segments of a single target polynucleotide are sequenced simultaneously.
5604098 M E T H O D S AND M A T E R I A L S FOR RESTRICTION ENDONUCLEASE APPLICATIONS Mead David; Swaminathan Neela Madison, WI, UNITED STATES Assigned to Molecular Biology Resources Inc The present invention is directed to materials and methods for the quasi-random and complete fragmentation of DNA using restriction endonuclease reagents capable of cutting DNA at a dinucleotide sequence. The invention is also directed to methods for labeling DNA using template-specific oligonucleotides, for shotgun cloning, for sequencing of DNA, for epitope mapping and for anonymous primer cloning, all using fragments of DNA generated by the method of the present invention.
5604099 PROCESS FOR D E T E C T I N G SPECIFIC N U C L E O T I D E VARIATIONS AND GENETIC P O L Y M O R P H I S M S PRESENT IN NUCLEIC ACIDS Erlich Henry A; Horn Glenn; Saiki Randall K; Mullis Kary Oakland, CA, UNITED STATES Assigned to Hoffmann-La Roche lnc
The bis-naphthalimides provided herein have exceptional DNA binding properties and demonstrate cytotoxicity in both in vitro and in vivo tumor models, in particular, against melanoma.
5604096 FLUOROMETRIC Q U A N T I T A T I O N OF MYCOPLASMAS Schaeffer Warren; Melamede Rober Burlington, VT, UNITED STATES The present invention pertains to methods of monitoring mycoplasmal growth whereby a culture of mycoplasmal cells is contacted with a fluorescent nucleic acid-binding agent under conditions which allow the agent to selectively bind to mycoplasmal nucleic acid. The amount of fluorescence associated with the mycoplasmal nucleic acid indicates the number of mycoplasmal cells in the culture. The fluorescence of the culture is typically read using a fluorometer which correlates with colonial growth on agar. A particularly preferred fluorescent nucleic acid-binding agent is ethidium bromide. The present invention further pertains to methods of determining the effectiveness of an antimycoplasmal antibiotic.
5604097 METHODS FOR SORTING P O L Y N U C L E O T I D E S USING O L I G O N U C L E O T I D E TAGS Brenner Sydney Cambridge, UNITED KINGDOM Assigned to Spectragen Inc The invention provides a method of tracking, identifying, and/or sorting classes or subpopulations of molecules by the use of oligonucleotide tags. Oligonucleotide tags of the invention each consist of a plurality of subunits 3 to 6 nucleotides in length selected from a minimally cross-hybridizing set. A subunit of a minimally cross-hybridizing set forms a duplex or triplex having two or more mismatches with the complement of any other subunit of the same set. The number of oligonucleotide tags available in a
737
particular embodiment depends on the number of subunits per tag and on the length of the subunit. An important aspect of the invention is the use of the oligonucleotide tags for sorting polynucleotides by specifically hybridizing tags attached to the polynucleotides to their complements on solid phase supports. This embodiment provides a readily automated system for manipulating and sorting polynucleotides, particularly useful in large-scale parallel operations, such as large-scale DNA sequencing, mRNA fingerprinting, and the like, wherein many target polynucleotides or many segments of a single target polynucleotide are sequenced simultaneously.
5604098 M E T H O D S AND M A T E R I A L S FOR RESTRICTION ENDONUCLEASE APPLICATIONS Mead David; Swaminathan Neela Madison, WI, UNITED STATES Assigned to Molecular Biology Resources Inc The present invention is directed to materials and methods for the quasi-random and complete fragmentation of DNA using restriction endonuclease reagents capable of cutting DNA at a dinucleotide sequence. The invention is also directed to methods for labeling DNA using template-specific oligonucleotides, for shotgun cloning, for sequencing of DNA, for epitope mapping and for anonymous primer cloning, all using fragments of DNA generated by the method of the present invention.
5604099 PROCESS FOR D E T E C T I N G SPECIFIC N U C L E O T I D E VARIATIONS AND GENETIC P O L Y M O R P H I S M S PRESENT IN NUCLEIC ACIDS Erlich Henry A; Horn Glenn; Saiki Randall K; Mullis Kary Oakland, CA, UNITED STATES Assigned to Hoffmann-La Roche lnc