- Email: [email protected]
5606033 Displacement chromatography of proteins using low molecular weight anionic displacers
PATENT ABSTRACTS
5606032
749
5606035
PROCESS FOR PREPARING GLIAL MITOGENIC FACTORS Goodearl Andre; Stroobant Paul; Minghetti Luis; Waterfield Michael; Marchioni Mark; Chen Mario S; Hiles la Hertfordshire, UNITED KINGDOM Assigned to Ludwig Institute for Cancer Research; Cambridge Neuroscience The invention involves the purification of various gliai mitogenic factors. In one aspect of the invention, vertebrate brain material is extracted, and the extract is then subjected to chromatographic separation. The glial mitogenic factors which can be obtained include a basic polypeptide with a molecular weight of from about 30 to about 36 kD, by reference to SDS-PAGE, and a second basic polypeptide factor which is from about 55 to about 63 kD as determined by SDS-page.
SESAMINOL
GLUCOSIDES
Kawakishi Shunrou; Osawa Nishikamo gun, Aichi, JAPAN
Toshihiko
New sesaminol glucosides, which are glucosides of liguan compounds of a specified structure, are effective in preventing oxidation of lipids in an aqueous solvent. Such sesaminol glucosides are produced substantially in pure form by obtaining an aqueous extract from crashed and defatted sesame seeds, processing the extract with beta-ghcosidase to obtain a processed sesame product which is itself an effective anti-oxidant, and make separations by liquid chromatography under specified conditions.
5606042 GLYCINE ALPHA-D-GALACTOSIDASES Smith Daniel; Walker John Columbia, MO, UNITED STATES Assigned to The Curators of the University of Missouri
5606033 DISPLACEMENT CHROMATOGRAPHY PROTEINS
USING
OF
A DNA and amino acid sequences of Glycine alpha-D-galactosidase are provided as well as the DNA sequence and mature length amino acid sequence of Phaseolus.
LOW
MOLECULAR WEIGHT ANIONIC DISPLACERS Cramer Steven M; Moore James; Kundu Amitav; Li Yufei; Jayaraman Guhan Schenectady, NY, UNITED STATES A method for the purification of proteins by displacement chromatography on ion exchange media using low molecular weight displacers is disclosed. Several classes of low molecular weight anionic species are exemplified, including aromatic rings having sulfonic acid or carboxylic acid moieties attached thereon, sulfated sugar derivatives, anionic antibiotics, and dendrimeric polymers. Novel compounds useful as displacers are dendrimers of formula (*See Patent for Chemical Structure*) wherein RI is lower alkyl, n is 2 to 6 and similar dendritic polymers based thereon.
5606116 CHROMATOGENOUS TESTING SYSTEM FOR URINALYSIS Yoneda Yuko; Itooka Toshiyuk Toyama, JAPAN Assigned to Kabushiki Kaisha Nippon Gene A testing system for urinalysis is provided comprising a support member which carries a developing phase, a reagent carrying means, a urine applying section, and a urine-reagent developing area. In order to prevent inaccurate test results which may result from splashing the sample onto the device, a water-repellent agent is coated onto both sides of the reagent phase and the urine-reagent developing phase, as well as onto both sides of the support member where the
5606032
749
5606035
PROCESS FOR PREPARING GLIAL MITOGENIC FACTORS Goodearl Andre; Stroobant Paul; Minghetti Luis; Waterfield Michael; Marchioni Mark; Chen Mario S; Hiles la Hertfordshire, UNITED KINGDOM Assigned to Ludwig Institute for Cancer Research; Cambridge Neuroscience The invention involves the purification of various gliai mitogenic factors. In one aspect of the invention, vertebrate brain material is extracted, and the extract is then subjected to chromatographic separation. The glial mitogenic factors which can be obtained include a basic polypeptide with a molecular weight of from about 30 to about 36 kD, by reference to SDS-PAGE, and a second basic polypeptide factor which is from about 55 to about 63 kD as determined by SDS-page.
SESAMINOL
GLUCOSIDES
Kawakishi Shunrou; Osawa Nishikamo gun, Aichi, JAPAN
Toshihiko
New sesaminol glucosides, which are glucosides of liguan compounds of a specified structure, are effective in preventing oxidation of lipids in an aqueous solvent. Such sesaminol glucosides are produced substantially in pure form by obtaining an aqueous extract from crashed and defatted sesame seeds, processing the extract with beta-ghcosidase to obtain a processed sesame product which is itself an effective anti-oxidant, and make separations by liquid chromatography under specified conditions.
5606042 GLYCINE ALPHA-D-GALACTOSIDASES Smith Daniel; Walker John Columbia, MO, UNITED STATES Assigned to The Curators of the University of Missouri
5606033 DISPLACEMENT CHROMATOGRAPHY PROTEINS
USING
OF
A DNA and amino acid sequences of Glycine alpha-D-galactosidase are provided as well as the DNA sequence and mature length amino acid sequence of Phaseolus.
LOW
MOLECULAR WEIGHT ANIONIC DISPLACERS Cramer Steven M; Moore James; Kundu Amitav; Li Yufei; Jayaraman Guhan Schenectady, NY, UNITED STATES A method for the purification of proteins by displacement chromatography on ion exchange media using low molecular weight displacers is disclosed. Several classes of low molecular weight anionic species are exemplified, including aromatic rings having sulfonic acid or carboxylic acid moieties attached thereon, sulfated sugar derivatives, anionic antibiotics, and dendrimeric polymers. Novel compounds useful as displacers are dendrimers of formula (*See Patent for Chemical Structure*) wherein RI is lower alkyl, n is 2 to 6 and similar dendritic polymers based thereon.
5606116 CHROMATOGENOUS TESTING SYSTEM FOR URINALYSIS Yoneda Yuko; Itooka Toshiyuk Toyama, JAPAN Assigned to Kabushiki Kaisha Nippon Gene A testing system for urinalysis is provided comprising a support member which carries a developing phase, a reagent carrying means, a urine applying section, and a urine-reagent developing area. In order to prevent inaccurate test results which may result from splashing the sample onto the device, a water-repellent agent is coated onto both sides of the reagent phase and the urine-reagent developing phase, as well as onto both sides of the support member where the