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5606035 Sesaminol glucosides
760
PATENT ABSTRACTS
complexing agent are disclosed for the simultaneous cleaning and disinfecting of contact lens. Methods for a daily use regimen are also disclosed.
5605809 C O M P O S I T I O N S FOR THE DETECTION OF PRO'lEASES IN BIOLOGICAL SAMPLES A N D M E T H O D S OF USE T H E R E O F Komoriya Akira; Packard Beverly Rockville, MD, UNITED STATES Assigned to Oncoimmunin lnc
5605661 M E T H O D S OF USING LIQUID ENZYME COMPOSITIONS CONTAINING MIXED POLYOLS Asgharian Bahra; Hong Bor-Shyue Arlington, TX, UNITED STATES Assigned to Alcon Laboratories Inc Compositions containing a stable, liquid, ophthalmically acceptable enzyme and methods involving the combined use of these compositions with a polymeric anfimicrobial agent are disclosed for the simultaneous cleaning and disinfecting of contact lens. Methods for a daily use regimen are also disclosed.
The present invention provides for novel reagents whose fluorescence increases in the presence of particular proteases. The reagents comprise a characteristically folded peptide backbone each end of which is conjugated to a fluorophore. When the folded peptide is cleaved, as by digestion with a protease, the fluorophores provide a high intensity fluorescent signal at a visible wavelength. Because of their high fluorescence signal in the visible wavelengths, these protease indicators are parricularly well suited for detection of protease activity in biological samples, in particular in frozen tissue sections. Thus this invention also provides for methods of detecting protease activity in situ in frozen sections.
5606025 HEMOGLOBIN-ENZYME COMPLEXES 5605808 O N C O P R O T E I N PROTEIN KINASE Karin Michae; Hibi Masahik; Lin Anning San Diego, CA, UNITED STATES Assigned to The Regents of the University of California An isolated polypeptide (JNK) characterized by having a molecular weight of 46 kD as determined by reducing SDS-PAGE, having serine and threonine kinase activity, phosphorylating the c-Jun N-terminal activation domain and polynucleotide sequences and method of detection of JNK are provided herein. JNK phosphorylates c-Jun N-terminal activation domain which affects gene expression from AP-1 sites.
D~guinoFetice;ChangThomas M S Mon~eal, Q u e ~ c , CANADA Crosslinked hemoglobin preparations having chemically bound thereto superoxide dismutase (SOD) and catalase are provided, for use as oxygen carrying rescusitative fluids, e.g. blood substitutes. The enzymes crosslinked to the hemoglobin effectively scavenge super oxide and hydrogen peroxide to improve the performance of the fluid in respect of ischemia-reperfusion injury risks.
5606035 SESAMINOL GLUCOSIDES Kawakishi Shunrou; Osawa Nishikamo gun, Aichi, JAPAN
Toshihiko
PATENT ABSTRACTS
New sesaminol glucosides, which are glucosides of lignan compounds of a specified structure, are effective in preventing oxidation of lipids in an aqueous solvent. Such sesaminol glucosides are produced substantially in pure form by obtaining an aqueous extract from crushed and defatted sesame seeds, processing the extract with beta-glucosidase to obtain a processed sesame product which is itself an effective anti-oxidant, and make separations by liquid chromatography under specified conditions.
5606042 GLYCINE ALPHA-D-GALACTOSIDASES Smith Daniel; Walker John Columbia, MO, UNITED STATES Assigned to The Curators of the University of Missouri A DNA and amino acid sequences of Glycine alpha-D-galactosidase are provided as well as the DNA sequence and mature length amino acid sequence of Phaseolus.
761
A method for determining alpha-amylase activity which involves bringing a sample into contact with an alpha-giucosidase in the presence of an alpha-amylase substrate and optically determining a liberated label, the substrate being a maltooligosaccharide composed of at least 3 glucose units, whose reducing terminal glucose is bonded to an optically measurable label at the 1-position by alpha-glucoside linkage or beta-glucoside linkage, and whose non-reducing terminal glucose is modified by a substituent other than glucose, and the alpha-glucosidase being substantially capable of acting on glucose to which the label is bonded at the 1-position by alpha-glucoside linkage and on all maltooligosaccharides having 2 to 7 glucose units; and a reagent for determining alpha-amylase activity comprising the alpha-glucosidase and said alpha-amylase substrate. The present invention permits, in the determination of alpha-amylase activity, efficient auxiliary action of adjuvant enzyme, a determination with good sensitivity, and little reagent blank reaction.
5607850 ENZYME
FROM MICROBIAL SOUR
Assigned to Eli Lilly and Company
5607838 METHOD FOR DETERMINATION OF ALPHA-AMYLASE ACTIVITY Hattori Shizuo; Yamamoto Yoshihir; Sogabe Yukihiro; Emi Shigenori Tsuruga, JAPAN Assigned to Toyo Boseki Kabushild Kalsha
Phthalyl amidase is an enzyme previously unknown in the art that catalyzes removal of the phthalyl moiety from phthalyl-contalning amides. The current invention provides said phthalyl amidase, means for producing it by culturing the natural organism from which the activity was identified, and methods for using said phthalyl amidase to remove the phthalyl moiety from phthalyl-containing amides.
PATENT ABSTRACTS
complexing agent are disclosed for the simultaneous cleaning and disinfecting of contact lens. Methods for a daily use regimen are also disclosed.
5605809 C O M P O S I T I O N S FOR THE DETECTION OF PRO'lEASES IN BIOLOGICAL SAMPLES A N D M E T H O D S OF USE T H E R E O F Komoriya Akira; Packard Beverly Rockville, MD, UNITED STATES Assigned to Oncoimmunin lnc
5605661 M E T H O D S OF USING LIQUID ENZYME COMPOSITIONS CONTAINING MIXED POLYOLS Asgharian Bahra; Hong Bor-Shyue Arlington, TX, UNITED STATES Assigned to Alcon Laboratories Inc Compositions containing a stable, liquid, ophthalmically acceptable enzyme and methods involving the combined use of these compositions with a polymeric anfimicrobial agent are disclosed for the simultaneous cleaning and disinfecting of contact lens. Methods for a daily use regimen are also disclosed.
The present invention provides for novel reagents whose fluorescence increases in the presence of particular proteases. The reagents comprise a characteristically folded peptide backbone each end of which is conjugated to a fluorophore. When the folded peptide is cleaved, as by digestion with a protease, the fluorophores provide a high intensity fluorescent signal at a visible wavelength. Because of their high fluorescence signal in the visible wavelengths, these protease indicators are parricularly well suited for detection of protease activity in biological samples, in particular in frozen tissue sections. Thus this invention also provides for methods of detecting protease activity in situ in frozen sections.
5606025 HEMOGLOBIN-ENZYME COMPLEXES 5605808 O N C O P R O T E I N PROTEIN KINASE Karin Michae; Hibi Masahik; Lin Anning San Diego, CA, UNITED STATES Assigned to The Regents of the University of California An isolated polypeptide (JNK) characterized by having a molecular weight of 46 kD as determined by reducing SDS-PAGE, having serine and threonine kinase activity, phosphorylating the c-Jun N-terminal activation domain and polynucleotide sequences and method of detection of JNK are provided herein. JNK phosphorylates c-Jun N-terminal activation domain which affects gene expression from AP-1 sites.
D~guinoFetice;ChangThomas M S Mon~eal, Q u e ~ c , CANADA Crosslinked hemoglobin preparations having chemically bound thereto superoxide dismutase (SOD) and catalase are provided, for use as oxygen carrying rescusitative fluids, e.g. blood substitutes. The enzymes crosslinked to the hemoglobin effectively scavenge super oxide and hydrogen peroxide to improve the performance of the fluid in respect of ischemia-reperfusion injury risks.
5606035 SESAMINOL GLUCOSIDES Kawakishi Shunrou; Osawa Nishikamo gun, Aichi, JAPAN
Toshihiko
PATENT ABSTRACTS
New sesaminol glucosides, which are glucosides of lignan compounds of a specified structure, are effective in preventing oxidation of lipids in an aqueous solvent. Such sesaminol glucosides are produced substantially in pure form by obtaining an aqueous extract from crushed and defatted sesame seeds, processing the extract with beta-glucosidase to obtain a processed sesame product which is itself an effective anti-oxidant, and make separations by liquid chromatography under specified conditions.
5606042 GLYCINE ALPHA-D-GALACTOSIDASES Smith Daniel; Walker John Columbia, MO, UNITED STATES Assigned to The Curators of the University of Missouri A DNA and amino acid sequences of Glycine alpha-D-galactosidase are provided as well as the DNA sequence and mature length amino acid sequence of Phaseolus.
761
A method for determining alpha-amylase activity which involves bringing a sample into contact with an alpha-giucosidase in the presence of an alpha-amylase substrate and optically determining a liberated label, the substrate being a maltooligosaccharide composed of at least 3 glucose units, whose reducing terminal glucose is bonded to an optically measurable label at the 1-position by alpha-glucoside linkage or beta-glucoside linkage, and whose non-reducing terminal glucose is modified by a substituent other than glucose, and the alpha-glucosidase being substantially capable of acting on glucose to which the label is bonded at the 1-position by alpha-glucoside linkage and on all maltooligosaccharides having 2 to 7 glucose units; and a reagent for determining alpha-amylase activity comprising the alpha-glucosidase and said alpha-amylase substrate. The present invention permits, in the determination of alpha-amylase activity, efficient auxiliary action of adjuvant enzyme, a determination with good sensitivity, and little reagent blank reaction.
5607850 ENZYME
FROM MICROBIAL SOUR
Assigned to Eli Lilly and Company
5607838 METHOD FOR DETERMINATION OF ALPHA-AMYLASE ACTIVITY Hattori Shizuo; Yamamoto Yoshihir; Sogabe Yukihiro; Emi Shigenori Tsuruga, JAPAN Assigned to Toyo Boseki Kabushild Kalsha
Phthalyl amidase is an enzyme previously unknown in the art that catalyzes removal of the phthalyl moiety from phthalyl-contalning amides. The current invention provides said phthalyl amidase, means for producing it by culturing the natural organism from which the activity was identified, and methods for using said phthalyl amidase to remove the phthalyl moiety from phthalyl-containing amides.