- Email: [email protected]
564 NEOADJUVANT SORAFENIB DECREASES T-REGULATORY CELLS INTRATUMORALLY IN RENAL CELL CARCINOMA (RCC) PATIENTS
564
NEOADJUVANT SORAFENIB DECREASES T-REGULATORY CELLS INTRATUMORALLY IN RENAL CELL CARCINOMA (RCC) PATIENTS
6WLOOHEURHU$-DFREV-)0'HVDU,0(0XOGHUV3)$ 9DQ'HU*UDDI:7$'H9ULHV,-09DQ+HUSHQ&0/ 1 Radboud University Nijmegen Medical Centre, Dept. of Urology, Nijmegen, The Netherlands, 2Radboud University Nijmegen Medical Center, Dept. of Tumor Immunologic Laboratory, Nijmegen, The Netherlands, 3Radboud University Nijmegen Medical Center, Dept. of Medical Oncology, Nijmegen, The Netherlands, 4Radboud University Nijmegen Medical Center, Dept. of Urology, Nijmegen, The Netherlands Introduction & Objectives: 5&& HYDGHV LPPXQH GHVWUXFWLRQ E\ UHFUXLWPHQW RI LPPXQRVXSSUHVVLYH FHOOV VXFK DV 7UHJV FKDUDFWHUL]HG DV &')R[3&'KLJK&'ORZ 7 FHOOV 5&& SDWLHQWV KDYH LQFUHDVHG QXPEHUV RI 7UHJVLQERWKSHULSKHUDOEORRGDQGWXPRUVZKLFKLVLQYHUVHO\FRUUHODWHGZLWKRYHUDOO VXUYLYDO9(*)5LQKLELWRUVFDQUHGXFHWKHQXPEHURI7UHJVLQWKHSHULSKHUDOEORRG 7KHLUHᚎHFWRQ7UHJVLQWUDWXPRUDOO\LQ5&&LVXQNQRZQ:HLQYHVWLJDWHGWKHHᚎHFW RIQHRDGMXYDQWWUHDWPHQWZLWKVRUDIHQLERQ7UHJLQᚏOWUDWLRQLQ5&& Material & Methods: 3UHYLRXVO\ XQWUHDWHG 5&& SDWLHQWV Q SDUWLFLSDWHG LQ DVWXG\LQZKLFKWKH\UHFHLYHGQHRDGMXYDQWVRUDIHQLEPJELGIRUZHHNV IROORZHG E\ D WXPRUQHSKUHFWRP\ %ORRG VDPSOHV DQG IUHVKO\ UHVHFWHG WXPRXU WLVVXH ZHUH FROOHFWHG $V D FRQWURO EORRG DQG 5&& VDPSOHV ZHUH FROOHFWHG RI SDWLHQWV Q ZKRZHUHQRWZLOOLQJWRSDUWLFLSDWHLQWKLVQHRDGMXYDQWVRUDIHQLE VWXG\ :H DOVR FROOHFWHG DVFLWHV Q SOHXUDO HᚎXVLRQV Q DQG PHWDVWDVHV Q RIXQWUHDWHG5&&SDWLHQWV3%0&VDQGWXPRXULQᚏOWUDWLQJO\PSKRF\WHVZHUH LVRODWHG )ORZ F\WRPHWULF DQDO\VLV ZDV SHUIRUPHG ZLWK D )$&6&DOLEXU XVLQJ GLUHFWO\ ODEHOOHG P$EV DJDLQVW &' &' &' &' &7/$ DQG )R[3 $ VXSSUHVVLRQDVVD\ZDVSHUIRUPHG Results: 7UHJVVSHFLᚏFDOO\DFFXPXODWHLQSULPDU\5&&V PHDQUDQJH PHWDVWDVHV PHDQ DVFLWHV PHDQUDQJH DQG LQ SOHXUDO HᚎXVLRQV PHDQ FRPSDUHG WR EORRG PHDQ 1HRDGMXYDQW VRUDIHQLE WUHDWPHQW Q VLJQLᚏFDQWO\ UHGXFHG WKH SHUFHQWDJH RI LQWUDWXPRUDO 7UHJV PHDQUDQJHS 3HULSKHUDOEORRG7UHJVLQFUHDVHG VOLJKWO\ DIWHU VRUDIHQLE WUHDWPHQW PHDQ YV S DOWKRXJK WKH FOLQLFDOUHOHYDQFHRIWKLVVHHPVOLPLWHG%RWKEORRGDQG5&&GHULYHG7UHJVZHUH IXQFWLRQDODVWKH\IXOO\DEURJDWH&'HᚎHFWRU7FHOOSUROLIHUDWLRQZLWKDVXEVHTXHQW VKDUSGHFUHDVHLQVHFUHWLRQRI,)1ˠDQG,/ Conclusions: 7UHJV DFFXPXODWH LQ 5&&V FRPSDUHG WR EORRG 1HRDGMXYDQW WUHDWPHQW ZLWK VRUDIHQLE LQGXFHG D VLJQLᚏFDQW GHFUHDVH RI LQWUDWXPRUDO 7UHJV LQ 5&&7KLVSURYLGHVDEDVLVIRUWKHUDWLRQDOHRIWKHFRPELQDWLRQRIVRUDIHQLEDQG LPPXQRWKHUDS\LQ5&&
565
PREVALENCE AND PROGNOSTIC SIGNIFICANCE OF ALPHA-METHYL COA RACEMASE (AMACR) EXPRESSION IN 1000 RENAL TUMOURS
(LFKHOEHUJ&6FKORPP7,VEDUQ+0LQQHU60RFK+7HUUDFFLDQR/ .HOO$+HXHU5&KXQ).5LQN0+HLQ]HU+)LVFK06LPRQ5 6DXWHU* 1 University Medical Center Hamburg-Eppendorf, Dept. of Urology, Hamburg, Germany, 2University Medical Center Hamburg-Eppendorf, Martini-Clinic Prostate Cancer Center, Hamburg, Germany, 3University Medical Center HamburgEppendorf, Dept. of Pathology, Hamburg, Germany, 4University Hospital Zurich, Dept. of Surgical Pathology, Zurich, Switzerland, 5University Hospital Basel, Dept. of Molecular Pathology, Zurich, Switzerland Introduction & Objectives: $0$&5 VWDLQLQJ ZDV SUHYLRXVO\ GHVFULEHG DV D XVHIXO PDUNHU IRU SDSLOODU\ UHQDO FHOO FDUFLQRPD SDS5&& VXEW\SLQJ +RZHYHU WKHLQYHVWLJDWHGJURXSVZHUHVPDOODQGᚏQGLQJVZHUHQRWFRUUHODWHGZLWKFOLQLFDO IHDWXUHV 7KH DLP RI RXU VWXG\ ZDV WR LQYHVWLJDWH WKH SUHYDOHQFH RI $0$&5 LPPXQRVWDLQLQJ ,+& LQ YDULRXV UHQDO OHVLRQV DQG WR DVVHVV LWV SURJQRVWLF UHOHYDQFH Material & Methods: $ELLQVWLWXWLRQDOUHQDOWXPRUWLVVXHPLFURDUUD\ 70$ RI WXPRU VDPSOHV SULPDU\ 5&& EHQLJQ DQG PDOLJQ UHQDO OHVLRQV DQG 5&&PHWDVWDVHV ZDVLQYHVWLJDWHGE\$0$&5,+&5HVXOWVZHUHFRUUHODWHGZLWK SDWKRORJLFDOIHDWXUHVDQGFOLQLFDOIROORZXSLQIRUPDWLRQ Results: 3DS5&& Q ZHUH IRXQG VLJQLᚏFDQWO\ PRUH RIWHQ $0$&5 SRVLWLYH FRPSDUHGWRRWKHU5&&KLVWRORJLFDOVXEW\SHV S RURWKHU NLGQH\OHVLRQV ([FOXVLYHO\LQSDS5&&$0$&5SRVLWLYLW\FRUUHODWHGZLWK VWDJH S EXW QRW ZLWK JUDGH QRGDO RU RUJDQ PHWDVWDVHV 1R VLJQLᚏFDQW FRUUHODWLRQ ZDV VHHQ IRU $0$&5 ,+& DQG RYHUDOO 26 RU FDQFHU VSHFLᚏF VXUYLYDO &66 7KHUHZDVDWUHQGWRDSURORQJHG\HDU&66UDWHIRU$0$&5 SRVLWLYHSDS5&&FRPSDUHGWRQHJDWLYHSDS5&& YV 2QPXOWLYDULDWH DQDO\VLVZLWKVWDJHQRGDOVWDWXVJUDGHDQGDJH$0$&5VWDLQLQJGLGQRWUHDFK LQGHSHQGHQWSUHGLFWRUVWDWXVRI26
Conclusions: $0$&5VWDLQLQJGLVFULPLQDWHVSDS5&&IURPRWKHUUHQDOWXPRXUV ZLWKDKLJKDFFXUDF\EXWGRHVQRWVLJQLᚏFDQWO\LPSDFWRQ26RU&66$VDUREXVW GLDJQRVWLFPDUNHUWRLGHQWLI\SDS5&&LWPLJKWSURYHLWVFOLQLFDOYDOXHLQHJUHQDO ELRSVLHVVWXGLHV
566
SERUM PRO-MATRIX METALLOPROTEINASE-7 (PROMMP-7) AS A PREDICTIVE MARKER FOR EARLY PROGRESSION AFTER NEPHRECTOMY IN RENAL CELL CARCINOMA
%LJRW3)HUJHORW35LRX[/HFOHUFT16DUNLVVLDQ*%HQVDODK./DP\ 3-&XOLQH67KXUHW5-RXLQ3'DUERXUHW%3DWDUG-- 1 Angers University Hospital, Dept. of Urology, Angers, France, 2University Hospital Center-Pontchaillou, Dept. of Biochemistry and Molecular Genetics, Rennes, France, 3University Hospital Center-Pontchaillou, Dept. of Pathology, Rennes, France, 4Parc Scientifique Georges Besse, Dept. of Research and Development, Nimes, France, 5University Hospital Center-Pontchaillou, Dept. of Urology, Rennes, France, 6Val D’Aurelle-Paul Lamarque Cancer Institute, Dept. of Clinical Laboratory, Montpellier, France, 7Montpellier University Hospital Center, Dept. of Urology, Montpellier, France, 8University of Montpellier 1, Dept. of CNRS, Montpellier, France Introduction & Objectives: ,QDSUHYLRXVVWXG\E\XVLQJSURWHRPLFDQDO\VLVZH LGHQWLᚏHGWKHSURPDWUL[P«WDOORSURW«LQDVH 3UR003 DVDVHUXPPDUNHURI UHQDOFHOOFDUFLQRPD 5&& 003FOHDYHVDEURDGUDQJHRIH[WUDFHOOXODUPDWUL[ PDFURPROHFXOHV VXFK DV ᚏEURQHFWLQ ODPLQLQ SURWHRJO\FDQ HODVWLQ JHODWLQ W\SH ,9 FROODJHQ DQG DOSKDDQWLWU\SVLQ WKXV SRWHQWLDOO\ SOD\LQJ DQ LPSRUWDQW UROH LQ ORFDOSURJUHVVLRQDQGGLVWDQWVSUHDGLQJ7KHREMHFWLYHRIWKLVVWXG\ZDVWRIXUWKHU H[DPLQHWKHSURJQRVWLFYDOXHRI3UR003LQDVHULHVRI5HQDO&HOO&DUFLQRPDV 5&& Material & Methods: SDWLHQWV ZKR XQGHUZHQW D QHSKUHFWRP\ IRU 5&& DW RXU LQVWLWXWLRQ ZHUH LQFOXGHG LQ WKLV SURVSHFWLYH VWXG\ $Q LQIRUPHG FRQVHQW IRU SDUWLFLSDWLQJLQWKHVWXG\ZDVREWDLQHGLQDOOFDVHV$OO3UR003VHUXPOHYHOV ZHUHGHWHUPLQHGEHIRUHQHSKUHFWRP\:HDQDO\VHGWKHDVVRFLDWLRQEHWZHHQ3UR 003OHYHOVDQGXVXDOFOLQLFRSDWKRORJLFDOSURJQRVWLFIDFWRUVLQ5&&4XDOLWDWLYH DQG TXDQWLWDWLYH YDULDEOHV ZHUH FRPSDUHG E\ XVLQJ FKLVTXDUH DQG 6WXGHQW VWDWLVWLFDODQDO\VHV3URJUHVVLRQIUHHDQG&DQFHUVSHFLᚏFVXUYLYDOZHUHDQDO\VHG E\WKH.DSODQ0HLHUPHWKRG Results: 7KHUH ZHUH PDOHV DQG IHPDOHV 0HGLDQ DJH DWGLDJQRVLVZDV\HDUV>@0HGLDQWXPRXUVL]HZDVFP>@1LQHW\ HLJKW DQG SDWLHQWV KDG ORFDOO\ DGYDQFHG 7 6WDJH ุ DQG KLJKJUDGHWXPRXUV )XKUPDQJUDGHุ UHVSHFWLYHO\7ZHQW\WKUHH DQG SDWLHQWVKDGQRGDODQGGLVWDQWPHWDVWDVHVUHVSHFWLYHO\0HGLDQ3UR 003 VHUXP YDOXH ZDV ˩J / >@ 0HGLDQ IROORZXS ZDV PRQWKV >@ $ VLJQLᚏFDQW DVVRFLDWLRQ ZDV IRXQG EHWZHHQ KLJK 3UR003 OHYHOV DQG SUHVHQFH RI WXPRXU UHODWHG V\PSWRPV ODUJHU WXPRXUV YV FP KLJKHU 7 DQG1VWDJHVKLJKHU)XKUPDQJUDGHVUHQDOYHLQLQYDVLRQ S 06WDJH LQIHULRUYHQDFDYDRUSHULQHSKULWLFIDWLQYDVLRQ S 3UR003OHYHOV DSSHDUHG WR EH KLJKHU LQ SDSLOODU\ WKDQ LQ FOHDU FHOO FDUFLQRPDV S ZKLOH DQ LQYHUVH WUHQG ZDV REVHUYHG IRU FKURPRSKREH FDUFLQRPDV S )LQDOO\ KLJKHU 3UR003 OHYHOV ZHUH DVVRFLDWHG ZLWK HDUO\ SURJUHVVLRQ S DQG SRRUFDQFHUVSHFLᚏFVXUYLYDO S Conclusions: 3UR003VHUXPPHDVXUHPHQWDSSHDUVWREHVWURQJO\DVVRFLDWHG ZLWKPRVWXVXDOFOLQLFDODQGSDWKRORJLFDOWXPRXUDJJUHVVLYHQHVVIHDWXUHVLQ5&& %DVHG RQ RXU UHVXOWV DQG EHFDXVH LW LV ELRORJLFDOO\ DVVRFLDWHG ZLWK GLVUXSWLRQ RI QDWXUDOEDUULHUVSUHYHQWLQJIURPWXPRXUVSUHDGLQJ003VKRXOGEHFRQVLGHUHG DVDPDUNHUIRUHDUO\SURJUHVVLRQDIWHUQHSKUHFWRP\,WFRXOGWKHUHIRUHEHFRPHD XVHIXOWRROIRUVHOHFWLQJSDWLHQWVZLWKDJJUHVVLYHWXPRXUVIRUDGMXYDQWWULDOV
567
ANALYSIS OF SFRP1 RNA EXPRESSION IN RENAL CELL CANCER AND ONCOCYTOMA AND ASSOCIATION WITH CLINICOPATHOLOGICAL PARAMETERS
$WVFKHN]HL)+HQQHQORWWHU--¦QLVFK6:DDONHV67H]YDO+ 0HUVHEXUJHU$6.XF]\N0$6HUWK- 1 Medizinische Hochschule Hannover, Dept. of Urology and Urological Oncology,
Eur Urol Suppl 2010;9(2):192
NEOADJUVANT SORAFENIB DECREASES T-REGULATORY CELLS INTRATUMORALLY IN RENAL CELL CARCINOMA (RCC) PATIENTS
6WLOOHEURHU$-DFREV-)0'HVDU,0(0XOGHUV3)$ 9DQ'HU*UDDI:7$'H9ULHV,-09DQ+HUSHQ&0/ 1 Radboud University Nijmegen Medical Centre, Dept. of Urology, Nijmegen, The Netherlands, 2Radboud University Nijmegen Medical Center, Dept. of Tumor Immunologic Laboratory, Nijmegen, The Netherlands, 3Radboud University Nijmegen Medical Center, Dept. of Medical Oncology, Nijmegen, The Netherlands, 4Radboud University Nijmegen Medical Center, Dept. of Urology, Nijmegen, The Netherlands Introduction & Objectives: 5&& HYDGHV LPPXQH GHVWUXFWLRQ E\ UHFUXLWPHQW RI LPPXQRVXSSUHVVLYH FHOOV VXFK DV 7UHJV FKDUDFWHUL]HG DV &')R[3&'KLJK&'ORZ 7 FHOOV 5&& SDWLHQWV KDYH LQFUHDVHG QXPEHUV RI 7UHJVLQERWKSHULSKHUDOEORRGDQGWXPRUVZKLFKLVLQYHUVHO\FRUUHODWHGZLWKRYHUDOO VXUYLYDO9(*)5LQKLELWRUVFDQUHGXFHWKHQXPEHURI7UHJVLQWKHSHULSKHUDOEORRG 7KHLUHᚎHFWRQ7UHJVLQWUDWXPRUDOO\LQ5&&LVXQNQRZQ:HLQYHVWLJDWHGWKHHᚎHFW RIQHRDGMXYDQWWUHDWPHQWZLWKVRUDIHQLERQ7UHJLQᚏOWUDWLRQLQ5&& Material & Methods: 3UHYLRXVO\ XQWUHDWHG 5&& SDWLHQWV Q SDUWLFLSDWHG LQ DVWXG\LQZKLFKWKH\UHFHLYHGQHRDGMXYDQWVRUDIHQLEPJELGIRUZHHNV IROORZHG E\ D WXPRUQHSKUHFWRP\ %ORRG VDPSOHV DQG IUHVKO\ UHVHFWHG WXPRXU WLVVXH ZHUH FROOHFWHG $V D FRQWURO EORRG DQG 5&& VDPSOHV ZHUH FROOHFWHG RI SDWLHQWV Q ZKRZHUHQRWZLOOLQJWRSDUWLFLSDWHLQWKLVQHRDGMXYDQWVRUDIHQLE VWXG\ :H DOVR FROOHFWHG DVFLWHV Q SOHXUDO HᚎXVLRQV Q DQG PHWDVWDVHV Q RIXQWUHDWHG5&&SDWLHQWV3%0&VDQGWXPRXULQᚏOWUDWLQJO\PSKRF\WHVZHUH LVRODWHG )ORZ F\WRPHWULF DQDO\VLV ZDV SHUIRUPHG ZLWK D )$&6&DOLEXU XVLQJ GLUHFWO\ ODEHOOHG P$EV DJDLQVW &' &' &' &' &7/$ DQG )R[3 $ VXSSUHVVLRQDVVD\ZDVSHUIRUPHG Results: 7UHJVVSHFLᚏFDOO\DFFXPXODWHLQSULPDU\5&&V PHDQUDQJH PHWDVWDVHV PHDQ DVFLWHV PHDQUDQJH DQG LQ SOHXUDO HᚎXVLRQV PHDQ FRPSDUHG WR EORRG PHDQ 1HRDGMXYDQW VRUDIHQLE WUHDWPHQW Q VLJQLᚏFDQWO\ UHGXFHG WKH SHUFHQWDJH RI LQWUDWXPRUDO 7UHJV PHDQUDQJHS 3HULSKHUDOEORRG7UHJVLQFUHDVHG VOLJKWO\ DIWHU VRUDIHQLE WUHDWPHQW PHDQ YV S DOWKRXJK WKH FOLQLFDOUHOHYDQFHRIWKLVVHHPVOLPLWHG%RWKEORRGDQG5&&GHULYHG7UHJVZHUH IXQFWLRQDODVWKH\IXOO\DEURJDWH&'HᚎHFWRU7FHOOSUROLIHUDWLRQZLWKDVXEVHTXHQW VKDUSGHFUHDVHLQVHFUHWLRQRI,)1ˠDQG,/ Conclusions: 7UHJV DFFXPXODWH LQ 5&&V FRPSDUHG WR EORRG 1HRDGMXYDQW WUHDWPHQW ZLWK VRUDIHQLE LQGXFHG D VLJQLᚏFDQW GHFUHDVH RI LQWUDWXPRUDO 7UHJV LQ 5&&7KLVSURYLGHVDEDVLVIRUWKHUDWLRQDOHRIWKHFRPELQDWLRQRIVRUDIHQLEDQG LPPXQRWKHUDS\LQ5&&
565
PREVALENCE AND PROGNOSTIC SIGNIFICANCE OF ALPHA-METHYL COA RACEMASE (AMACR) EXPRESSION IN 1000 RENAL TUMOURS
(LFKHOEHUJ&6FKORPP7,VEDUQ+0LQQHU60RFK+7HUUDFFLDQR/ .HOO$+HXHU5&KXQ).5LQN0+HLQ]HU+)LVFK06LPRQ5 6DXWHU* 1 University Medical Center Hamburg-Eppendorf, Dept. of Urology, Hamburg, Germany, 2University Medical Center Hamburg-Eppendorf, Martini-Clinic Prostate Cancer Center, Hamburg, Germany, 3University Medical Center HamburgEppendorf, Dept. of Pathology, Hamburg, Germany, 4University Hospital Zurich, Dept. of Surgical Pathology, Zurich, Switzerland, 5University Hospital Basel, Dept. of Molecular Pathology, Zurich, Switzerland Introduction & Objectives: $0$&5 VWDLQLQJ ZDV SUHYLRXVO\ GHVFULEHG DV D XVHIXO PDUNHU IRU SDSLOODU\ UHQDO FHOO FDUFLQRPD SDS5&& VXEW\SLQJ +RZHYHU WKHLQYHVWLJDWHGJURXSVZHUHVPDOODQGᚏQGLQJVZHUHQRWFRUUHODWHGZLWKFOLQLFDO IHDWXUHV 7KH DLP RI RXU VWXG\ ZDV WR LQYHVWLJDWH WKH SUHYDOHQFH RI $0$&5 LPPXQRVWDLQLQJ ,+& LQ YDULRXV UHQDO OHVLRQV DQG WR DVVHVV LWV SURJQRVWLF UHOHYDQFH Material & Methods: $ELLQVWLWXWLRQDOUHQDOWXPRUWLVVXHPLFURDUUD\ 70$ RI WXPRU VDPSOHV SULPDU\ 5&& EHQLJQ DQG PDOLJQ UHQDO OHVLRQV DQG 5&&PHWDVWDVHV ZDVLQYHVWLJDWHGE\$0$&5,+&5HVXOWVZHUHFRUUHODWHGZLWK SDWKRORJLFDOIHDWXUHVDQGFOLQLFDOIROORZXSLQIRUPDWLRQ Results: 3DS5&& Q ZHUH IRXQG VLJQLᚏFDQWO\ PRUH RIWHQ $0$&5 SRVLWLYH FRPSDUHGWRRWKHU5&&KLVWRORJLFDOVXEW\SHV S RURWKHU NLGQH\OHVLRQV ([FOXVLYHO\LQSDS5&&$0$&5SRVLWLYLW\FRUUHODWHGZLWK VWDJH S EXW QRW ZLWK JUDGH QRGDO RU RUJDQ PHWDVWDVHV 1R VLJQLᚏFDQW FRUUHODWLRQ ZDV VHHQ IRU $0$&5 ,+& DQG RYHUDOO 26 RU FDQFHU VSHFLᚏF VXUYLYDO &66 7KHUHZDVDWUHQGWRDSURORQJHG\HDU&66UDWHIRU$0$&5 SRVLWLYHSDS5&&FRPSDUHGWRQHJDWLYHSDS5&& YV 2QPXOWLYDULDWH DQDO\VLVZLWKVWDJHQRGDOVWDWXVJUDGHDQGDJH$0$&5VWDLQLQJGLGQRWUHDFK LQGHSHQGHQWSUHGLFWRUVWDWXVRI26
Conclusions: $0$&5VWDLQLQJGLVFULPLQDWHVSDS5&&IURPRWKHUUHQDOWXPRXUV ZLWKDKLJKDFFXUDF\EXWGRHVQRWVLJQLᚏFDQWO\LPSDFWRQ26RU&66$VDUREXVW GLDJQRVWLFPDUNHUWRLGHQWLI\SDS5&&LWPLJKWSURYHLWVFOLQLFDOYDOXHLQHJUHQDO ELRSVLHVVWXGLHV
566
SERUM PRO-MATRIX METALLOPROTEINASE-7 (PROMMP-7) AS A PREDICTIVE MARKER FOR EARLY PROGRESSION AFTER NEPHRECTOMY IN RENAL CELL CARCINOMA
%LJRW3)HUJHORW35LRX[/HFOHUFT16DUNLVVLDQ*%HQVDODK./DP\ 3-&XOLQH67KXUHW5-RXLQ3'DUERXUHW%3DWDUG-- 1 Angers University Hospital, Dept. of Urology, Angers, France, 2University Hospital Center-Pontchaillou, Dept. of Biochemistry and Molecular Genetics, Rennes, France, 3University Hospital Center-Pontchaillou, Dept. of Pathology, Rennes, France, 4Parc Scientifique Georges Besse, Dept. of Research and Development, Nimes, France, 5University Hospital Center-Pontchaillou, Dept. of Urology, Rennes, France, 6Val D’Aurelle-Paul Lamarque Cancer Institute, Dept. of Clinical Laboratory, Montpellier, France, 7Montpellier University Hospital Center, Dept. of Urology, Montpellier, France, 8University of Montpellier 1, Dept. of CNRS, Montpellier, France Introduction & Objectives: ,QDSUHYLRXVVWXG\E\XVLQJSURWHRPLFDQDO\VLVZH LGHQWLᚏHGWKHSURPDWUL[P«WDOORSURW«LQDVH 3UR003 DVDVHUXPPDUNHURI UHQDOFHOOFDUFLQRPD 5&& 003FOHDYHVDEURDGUDQJHRIH[WUDFHOOXODUPDWUL[ PDFURPROHFXOHV VXFK DV ᚏEURQHFWLQ ODPLQLQ SURWHRJO\FDQ HODVWLQ JHODWLQ W\SH ,9 FROODJHQ DQG DOSKDDQWLWU\SVLQ WKXV SRWHQWLDOO\ SOD\LQJ DQ LPSRUWDQW UROH LQ ORFDOSURJUHVVLRQDQGGLVWDQWVSUHDGLQJ7KHREMHFWLYHRIWKLVVWXG\ZDVWRIXUWKHU H[DPLQHWKHSURJQRVWLFYDOXHRI3UR003LQDVHULHVRI5HQDO&HOO&DUFLQRPDV 5&& Material & Methods: SDWLHQWV ZKR XQGHUZHQW D QHSKUHFWRP\ IRU 5&& DW RXU LQVWLWXWLRQ ZHUH LQFOXGHG LQ WKLV SURVSHFWLYH VWXG\ $Q LQIRUPHG FRQVHQW IRU SDUWLFLSDWLQJLQWKHVWXG\ZDVREWDLQHGLQDOOFDVHV$OO3UR003VHUXPOHYHOV ZHUHGHWHUPLQHGEHIRUHQHSKUHFWRP\:HDQDO\VHGWKHDVVRFLDWLRQEHWZHHQ3UR 003OHYHOVDQGXVXDOFOLQLFRSDWKRORJLFDOSURJQRVWLFIDFWRUVLQ5&&4XDOLWDWLYH DQG TXDQWLWDWLYH YDULDEOHV ZHUH FRPSDUHG E\ XVLQJ FKLVTXDUH DQG 6WXGHQW VWDWLVWLFDODQDO\VHV3URJUHVVLRQIUHHDQG&DQFHUVSHFLᚏFVXUYLYDOZHUHDQDO\VHG E\WKH.DSODQ0HLHUPHWKRG Results: 7KHUH ZHUH PDOHV DQG IHPDOHV 0HGLDQ DJH DWGLDJQRVLVZDV\HDUV>@0HGLDQWXPRXUVL]HZDVFP>@1LQHW\ HLJKW DQG SDWLHQWV KDG ORFDOO\ DGYDQFHG 7 6WDJH ุ DQG KLJKJUDGHWXPRXUV )XKUPDQJUDGHุ UHVSHFWLYHO\7ZHQW\WKUHH DQG SDWLHQWVKDGQRGDODQGGLVWDQWPHWDVWDVHVUHVSHFWLYHO\0HGLDQ3UR 003 VHUXP YDOXH ZDV ˩J / >@ 0HGLDQ IROORZXS ZDV PRQWKV >@ $ VLJQLᚏFDQW DVVRFLDWLRQ ZDV IRXQG EHWZHHQ KLJK 3UR003 OHYHOV DQG SUHVHQFH RI WXPRXU UHODWHG V\PSWRPV ODUJHU WXPRXUV YV FP KLJKHU 7 DQG1VWDJHVKLJKHU)XKUPDQJUDGHVUHQDOYHLQLQYDVLRQ S 06WDJH LQIHULRUYHQDFDYDRUSHULQHSKULWLFIDWLQYDVLRQ S 3UR003OHYHOV DSSHDUHG WR EH KLJKHU LQ SDSLOODU\ WKDQ LQ FOHDU FHOO FDUFLQRPDV S ZKLOH DQ LQYHUVH WUHQG ZDV REVHUYHG IRU FKURPRSKREH FDUFLQRPDV S )LQDOO\ KLJKHU 3UR003 OHYHOV ZHUH DVVRFLDWHG ZLWK HDUO\ SURJUHVVLRQ S DQG SRRUFDQFHUVSHFLᚏFVXUYLYDO S Conclusions: 3UR003VHUXPPHDVXUHPHQWDSSHDUVWREHVWURQJO\DVVRFLDWHG ZLWKPRVWXVXDOFOLQLFDODQGSDWKRORJLFDOWXPRXUDJJUHVVLYHQHVVIHDWXUHVLQ5&& %DVHG RQ RXU UHVXOWV DQG EHFDXVH LW LV ELRORJLFDOO\ DVVRFLDWHG ZLWK GLVUXSWLRQ RI QDWXUDOEDUULHUVSUHYHQWLQJIURPWXPRXUVSUHDGLQJ003VKRXOGEHFRQVLGHUHG DVDPDUNHUIRUHDUO\SURJUHVVLRQDIWHUQHSKUHFWRP\,WFRXOGWKHUHIRUHEHFRPHD XVHIXOWRROIRUVHOHFWLQJSDWLHQWVZLWKDJJUHVVLYHWXPRXUVIRUDGMXYDQWWULDOV
567
ANALYSIS OF SFRP1 RNA EXPRESSION IN RENAL CELL CANCER AND ONCOCYTOMA AND ASSOCIATION WITH CLINICOPATHOLOGICAL PARAMETERS
$WVFKHN]HL)+HQQHQORWWHU--¦QLVFK6:DDONHV67H]YDO+ 0HUVHEXUJHU$6.XF]\N0$6HUWK- 1 Medizinische Hochschule Hannover, Dept. of Urology and Urological Oncology,
Eur Urol Suppl 2010;9(2):192