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6.10. Use of isoelectric focusing in parentage testing
276
In the AMY, system sera of 204 persons (183 AMYs 1, 21 AMY2 2-l phenotypes) were analyzed by IEF. The allele frequencies of 0.949 (AMY;) and 0.051 (AMY;) were again in good accordance with those of other authors, who have employed the agarose gel technique. The Hardy-Weinberg equilibrium conditions were given (2:~ ’ = 0.03; 0.80 < p < 0.90; d.f. = 1); the single exclusion chance of the serum (pancreas) amylase was estimated at 4.6%. However, as both loci (AMY1 and AMY,) are very closely linked [2) a ‘super-haplotype’ similar to HLA-Bf should be considered in biostatistical calculations of paternity cases, although figures of linkage disequilibria are still lacking. References 1 P. Kiihnl 2 Kamaryt
6.10.
and H. Tischberger,
et al., Hum. Genet.,
USE OF ISOELECTRIC
1 (1980) Electrophoresis, 43 (1978) 205 - 210.
FOCUSING
H. F. Polesky and D. Dykes Minneapolis, MI 55404, U.S.A.)
186 - 190.
IN PARENTAGE
(Minneapolis
TESTING
War Memorial
Blood
Bank,
The routine use of isoelectric focusing (IEF) techniques for phenotyping PGM, and Cc was evaluated on samples in cases of disputed parentage involving 2500 Caucasian and 200 Black trios. The increased number of common phenotypes observed by IEF (PGM, from 3 to 10, Gc from 3 to 6) changes the theoretical exclusion probability P of these systems. In the PGM, system the P-value for Caucasians using starch techniques is 0.14 and by IEF is 0.29. In Blacks the value changes from 0.12 to 0.26. In the Gc system the expected P-value for Caucasians using immunofixation on agarose is 0.16 compared with a value of 0.31 by IEF. In Blacks the value changes from 0.10 to 0.30. In both systems the observed exclusions increased more than expected (Caucasians: PGMr 3.39% to 8.71%, Gc 3.62% to 8.38%; Blacks: PGMi 1.25% to 10.7%, Cc 2.29% to 10.1%). Additional advantages obtained by using IEF are larger values for the calculation of the paternity index in cases without exclusions and the ability to identify a large number of rare variants not found with conventional techniques.
6.11. THE COURSE
STABILITY
OF
SEMINAL
ENZYMES
IN THE
B. H. Parkin (The Metropolitan Police Forensic Lambeth Road, London SE1 7LP, Gt. Britain)
VAGINA
Science
AFTER
Laboratory,
INTER-
109
In the AMY, system sera of 204 persons (183 AMYs 1, 21 AMY2 2-l phenotypes) were analyzed by IEF. The allele frequencies of 0.949 (AMY;) and 0.051 (AMY;) were again in good accordance with those of other authors, who have employed the agarose gel technique. The Hardy-Weinberg equilibrium conditions were given (2:~ ’ = 0.03; 0.80 < p < 0.90; d.f. = 1); the single exclusion chance of the serum (pancreas) amylase was estimated at 4.6%. However, as both loci (AMY1 and AMY,) are very closely linked [2) a ‘super-haplotype’ similar to HLA-Bf should be considered in biostatistical calculations of paternity cases, although figures of linkage disequilibria are still lacking. References 1 P. Kiihnl 2 Kamaryt
6.10.
and H. Tischberger,
et al., Hum. Genet.,
USE OF ISOELECTRIC
1 (1980) Electrophoresis, 43 (1978) 205 - 210.
FOCUSING
H. F. Polesky and D. Dykes Minneapolis, MI 55404, U.S.A.)
186 - 190.
IN PARENTAGE
(Minneapolis
TESTING
War Memorial
Blood
Bank,
The routine use of isoelectric focusing (IEF) techniques for phenotyping PGM, and Cc was evaluated on samples in cases of disputed parentage involving 2500 Caucasian and 200 Black trios. The increased number of common phenotypes observed by IEF (PGM, from 3 to 10, Gc from 3 to 6) changes the theoretical exclusion probability P of these systems. In the PGM, system the P-value for Caucasians using starch techniques is 0.14 and by IEF is 0.29. In Blacks the value changes from 0.12 to 0.26. In the Gc system the expected P-value for Caucasians using immunofixation on agarose is 0.16 compared with a value of 0.31 by IEF. In Blacks the value changes from 0.10 to 0.30. In both systems the observed exclusions increased more than expected (Caucasians: PGMr 3.39% to 8.71%, Gc 3.62% to 8.38%; Blacks: PGMi 1.25% to 10.7%, Cc 2.29% to 10.1%). Additional advantages obtained by using IEF are larger values for the calculation of the paternity index in cases without exclusions and the ability to identify a large number of rare variants not found with conventional techniques.
6.11. THE COURSE
STABILITY
OF
SEMINAL
ENZYMES
IN THE
B. H. Parkin (The Metropolitan Police Forensic Lambeth Road, London SE1 7LP, Gt. Britain)
VAGINA
Science
AFTER
Laboratory,
INTER-
109