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612 Allergy to chicory (Witloof)
Abstracts
J ALLERGY CLIN IMMUNOL VOLUME 97, NUMBER 1, PART3
609
610
Study of allergenicity of peel and pulp from Rosaceae fruits. M F e r n ~ n d e z - R i v a s MD PhD (I). A M a ~ t f n e z PhD (2). M CuevamMD PhD (3), Avila (I), Bilbao (2), Madrid (3), Spain. The aim of the study was to compare the allergenicity o f p e e l and p u l p o f a p p l e , p e a r and p e a c h . We i n c l u d e d 22 p a t i e n t s a l l e r g i c t o t h e s e fruits. They a l l r e a c t e d w i t h t h e whole f r u i t ( p e e l + p u l p ) , b u t some o f them t o l e r a t e d t h e i n t a k e o f t h e p u l p . An a s s o c i a t i o n (X6p<.05) was found between the ingestion o f t h e p e e l and t h e a p p e a r a n c e o f a r e a c t i o n . S k i n p r i c k t e s t s (SPT) t o t h e p e e l s o f t h e t h r e e f r u i t s were g r e a t e r t h a n those of their pulps (Wilcoxon test p<.01). No difference was found between peel and pulp by RAST. For each fruit the peel RAST was inhibited by itself and by its pulp, and viceversa. In all the assays performed an important inhibition was obtained (67-91~), and the inhibition lines had similar slopes. The 50~ inhibitory concentrations for pulps were 5 to 34 fold-higher than those of peels. SDS-PAGE and IgE immunoblottings showed a similar pattern for peel and pulp from the same species. Mutual immunoblotting inhibition was found between peel and pulp for the three fruits. Our r e s u l t s s u g g e s t t h a t peels o f R o s a c e a e f r u i t s a r e more a l l e r g e n i c t h a n p u l p s b e c a u s e o f a higher concentration of allergens. This fact must be t a k e n i n t o a c c o u n t f o r SPT w i t h f r e s h f r u i t s , and t o e l a b o r a t e d i a g n o s t i c m a t e r i a l s .
611
IgE antibodies cross-reaction between Der pl, the major allergen of Dermatophagoides pteronyssinus and Ananain, a cysteine proteinase from A n a n a s e o m o s u s , the pineapple plant.
612
T Musu BS. J RabiUon. B David MD. JP Dandeu Phl). LVITGuinnepain M D and Y Looze PhD. Paris, France The finding that major allergens of some dust nfites (1). pteronyssinus, D. farinae ) and of soya beans (Glycine max ) belong to the cysteine proteinase supeff~anilyand the recent developing of Spagylation technology (1) for the very high purification of the these enzymes prompted us to examine the cross-reaction of specific IgE antibodies to Der pl and Ananain from Ananas comosus. Elisa inhibition assays were performed on a group of 5 patients suffering from mite hypersensitivity. The absolute amount of protein causing 50% inhibition of lgE-binding to the solid phase were calculated in homologous and heterologous systems from dose-related inhibition graphs. When Der pl was coated, the amount of Ananaln to reach 50% inhibition was in average 100-fold greater than the amount of the homologous protein. Interestingly, when Ananain was coated the amount of Der pl to reach 50% inhibition was in all sent tested nearly identical to the amount of Ananain to reach the same inhibition. No inhibition was observed in both systems when Fel dl was used as the control inhibitor. Also, specific inhibitors were used to ensure that no proteolytic activity of Der pl or Auauain could interfere with inhibition. Taken together, the results support the view tlmt epitopes of Der pl are present on Auauain and that, compared to Der pl, the allergen-specific IgE of the patients have a lower affmity to Auauain. These results raise interesting questions about the way of sensizimtion to cysteine proteinases since Ananain is one of the components of Bromelain, a mixture of enzymes from the pineapple stem, commonly meal for meat tendexization or beer clarification. (1) T Musu et al. IIBC 1994; 1:17-27
335
Immunological cross-reactivity of apple 18 kD allergen and constituents of common vegetables L-S Hsiel~ PhD. B. Martin.. Phi), L. Hadswi~er*. PkD. Bethesda. Cross-reactive allergens may be responsible for patients' allergy to fruits and vegetables. We have studied the apple 18 kD allergen and cross reactive proteins in various vegetables using immunoblotting and inhibition immunoblotting. The presence of the apple 18 kD protein was reported to be directly proportional to its allergenic potency by in vivo assay (Allergy 1994;49:262-271), and its amino acid sequence is homologous to a wide-spread protein related to pathogenesis-related proteins, PI49. Cross reactivity of sixteen common vegetables, which may possess this protein, was determined. Vegetable extracts were prepared in alkaline buffer with polyvinylpolypyrrolidone (PVPP), EDTA, and diethyldithiocarbamic acid (DIECA). Using serum specific to the apple 18 kD protein, a serum pool of apple allergic patients, and antiserum to PI49, the reactivity among vegetables was evaluated. The apple 18 kD protein cross reacts with 16-18 kD proteins of eggplant, carrot, pea, green bean, and corn. The PI49 protein in peas and beaus is inducible when challenged with Fusarium solantf, sp., but is not inducible in the other vegetables. The IgE reactivity by immunoblot of apple 18 kD protein can be completely inhibited by extract of eggplant, and partially inhibited by extract of carrot, corn, and infected peas, suggesting complete or partial cross reactivity. Further analyses and characterization of this protein are underway. The cross reactivity of these pathogenesis-related proteins may explain clinical observations of reactivity of multiple food allergens.
Allergy to C h i c o r y (Witloof) A Helblin~ M D . A Reimers MD. M W~ilti PhD. R Borats MD_, Bern, Switzerland. Chicory [C](Cichorium intybus), a c o m m o n salad plant, belongs to the large family of Asteraceae. Whereas mugwort in Europe and ragweed in the U.S., both non-food members ot the Asteraceae, are well-known causes o f respiratory allergy, foods o f this family such as lettuce, endive, artichoke or oster plant rarely induce hypersensitivity. A 26-year-old female cook with a history of hay fever has been referred to our clinic because o f recurred systemic reactions (rhinitis, angioedema, urticaria, dysphagia) following ingestion of C. W h e n handling C, she experienced itching and contact urticaria. SPT were positive to grass pollen, latex, kiwi, celery stalk, fresh C and lettuce leafs, but negative to mugwort and ragweed pollen. R A S T ' s were positive to celery (class 2) and latex (class 1), but negative to C, Bet v 1 and Bet v 2 (Profilin). The patient reacted to the oral intake of 20 g fresh C with a rhinoconjunctivitis, palmar rash, dysphagia, and a generalized urticaria 10 minutes after the challenge. Although foods of the Asteraceous family are commonly considered as hypoallergenic, this report suggests that C may elicit IgE-mediated food allergy symptoms.
J ALLERGY CLIN IMMUNOL VOLUME 97, NUMBER 1, PART3
609
610
Study of allergenicity of peel and pulp from Rosaceae fruits. M F e r n ~ n d e z - R i v a s MD PhD (I). A M a ~ t f n e z PhD (2). M CuevamMD PhD (3), Avila (I), Bilbao (2), Madrid (3), Spain. The aim of the study was to compare the allergenicity o f p e e l and p u l p o f a p p l e , p e a r and p e a c h . We i n c l u d e d 22 p a t i e n t s a l l e r g i c t o t h e s e fruits. They a l l r e a c t e d w i t h t h e whole f r u i t ( p e e l + p u l p ) , b u t some o f them t o l e r a t e d t h e i n t a k e o f t h e p u l p . An a s s o c i a t i o n (X6p<.05) was found between the ingestion o f t h e p e e l and t h e a p p e a r a n c e o f a r e a c t i o n . S k i n p r i c k t e s t s (SPT) t o t h e p e e l s o f t h e t h r e e f r u i t s were g r e a t e r t h a n those of their pulps (Wilcoxon test p<.01). No difference was found between peel and pulp by RAST. For each fruit the peel RAST was inhibited by itself and by its pulp, and viceversa. In all the assays performed an important inhibition was obtained (67-91~), and the inhibition lines had similar slopes. The 50~ inhibitory concentrations for pulps were 5 to 34 fold-higher than those of peels. SDS-PAGE and IgE immunoblottings showed a similar pattern for peel and pulp from the same species. Mutual immunoblotting inhibition was found between peel and pulp for the three fruits. Our r e s u l t s s u g g e s t t h a t peels o f R o s a c e a e f r u i t s a r e more a l l e r g e n i c t h a n p u l p s b e c a u s e o f a higher concentration of allergens. This fact must be t a k e n i n t o a c c o u n t f o r SPT w i t h f r e s h f r u i t s , and t o e l a b o r a t e d i a g n o s t i c m a t e r i a l s .
611
IgE antibodies cross-reaction between Der pl, the major allergen of Dermatophagoides pteronyssinus and Ananain, a cysteine proteinase from A n a n a s e o m o s u s , the pineapple plant.
612
T Musu BS. J RabiUon. B David MD. JP Dandeu Phl). LVITGuinnepain M D and Y Looze PhD. Paris, France The finding that major allergens of some dust nfites (1). pteronyssinus, D. farinae ) and of soya beans (Glycine max ) belong to the cysteine proteinase supeff~anilyand the recent developing of Spagylation technology (1) for the very high purification of the these enzymes prompted us to examine the cross-reaction of specific IgE antibodies to Der pl and Ananain from Ananas comosus. Elisa inhibition assays were performed on a group of 5 patients suffering from mite hypersensitivity. The absolute amount of protein causing 50% inhibition of lgE-binding to the solid phase were calculated in homologous and heterologous systems from dose-related inhibition graphs. When Der pl was coated, the amount of Ananaln to reach 50% inhibition was in average 100-fold greater than the amount of the homologous protein. Interestingly, when Ananain was coated the amount of Der pl to reach 50% inhibition was in all sent tested nearly identical to the amount of Ananain to reach the same inhibition. No inhibition was observed in both systems when Fel dl was used as the control inhibitor. Also, specific inhibitors were used to ensure that no proteolytic activity of Der pl or Auauain could interfere with inhibition. Taken together, the results support the view tlmt epitopes of Der pl are present on Auauain and that, compared to Der pl, the allergen-specific IgE of the patients have a lower affmity to Auauain. These results raise interesting questions about the way of sensizimtion to cysteine proteinases since Ananain is one of the components of Bromelain, a mixture of enzymes from the pineapple stem, commonly meal for meat tendexization or beer clarification. (1) T Musu et al. IIBC 1994; 1:17-27
335
Immunological cross-reactivity of apple 18 kD allergen and constituents of common vegetables L-S Hsiel~ PhD. B. Martin.. Phi), L. Hadswi~er*. PkD. Bethesda. Cross-reactive allergens may be responsible for patients' allergy to fruits and vegetables. We have studied the apple 18 kD allergen and cross reactive proteins in various vegetables using immunoblotting and inhibition immunoblotting. The presence of the apple 18 kD protein was reported to be directly proportional to its allergenic potency by in vivo assay (Allergy 1994;49:262-271), and its amino acid sequence is homologous to a wide-spread protein related to pathogenesis-related proteins, PI49. Cross reactivity of sixteen common vegetables, which may possess this protein, was determined. Vegetable extracts were prepared in alkaline buffer with polyvinylpolypyrrolidone (PVPP), EDTA, and diethyldithiocarbamic acid (DIECA). Using serum specific to the apple 18 kD protein, a serum pool of apple allergic patients, and antiserum to PI49, the reactivity among vegetables was evaluated. The apple 18 kD protein cross reacts with 16-18 kD proteins of eggplant, carrot, pea, green bean, and corn. The PI49 protein in peas and beaus is inducible when challenged with Fusarium solantf, sp., but is not inducible in the other vegetables. The IgE reactivity by immunoblot of apple 18 kD protein can be completely inhibited by extract of eggplant, and partially inhibited by extract of carrot, corn, and infected peas, suggesting complete or partial cross reactivity. Further analyses and characterization of this protein are underway. The cross reactivity of these pathogenesis-related proteins may explain clinical observations of reactivity of multiple food allergens.
Allergy to C h i c o r y (Witloof) A Helblin~ M D . A Reimers MD. M W~ilti PhD. R Borats MD_, Bern, Switzerland. Chicory [C](Cichorium intybus), a c o m m o n salad plant, belongs to the large family of Asteraceae. Whereas mugwort in Europe and ragweed in the U.S., both non-food members ot the Asteraceae, are well-known causes o f respiratory allergy, foods o f this family such as lettuce, endive, artichoke or oster plant rarely induce hypersensitivity. A 26-year-old female cook with a history of hay fever has been referred to our clinic because o f recurred systemic reactions (rhinitis, angioedema, urticaria, dysphagia) following ingestion of C. W h e n handling C, she experienced itching and contact urticaria. SPT were positive to grass pollen, latex, kiwi, celery stalk, fresh C and lettuce leafs, but negative to mugwort and ragweed pollen. R A S T ' s were positive to celery (class 2) and latex (class 1), but negative to C, Bet v 1 and Bet v 2 (Profilin). The patient reacted to the oral intake of 20 g fresh C with a rhinoconjunctivitis, palmar rash, dysphagia, and a generalized urticaria 10 minutes after the challenge. Although foods of the Asteraceous family are commonly considered as hypoallergenic, this report suggests that C may elicit IgE-mediated food allergy symptoms.