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65. Rapid thawing of frozen canine kidneys by microwaves
:\ N N[ ;AL M 17I'71"IN (_, .\ B5 1 .H A (, ! a ling :-tlone w e r v "tts~ slu~lfl'd. V:trious degrees of s t r u e t t t r a l i~tt,~t'it3" (,f t l.:~ ki
k.itlfl('Vs
,+lll;tSiOltl(ist,tl
(,xtr,'/-
+';Wl~rt,:illv f(~t" 6 hrs i~tdie:l.!es ri,t~,tltiml of hiot,:..zie:~l ['tttwth>tls in ~-:ev('t':il c:tst's' v,'llMt was :~ls<:, ih,'
(~l>set'v-~tiolt
I(]fl~-l{'rlll
of
:-( ,vt't,t[" ~tni~tt:'lflt-
~h),..+s ',xilh tlw reimfd:~ntt,~t k M t w v :tl'T~+I" l l'(h'iIlllt'll|, llllt]f'l' high l~r('s~ll'C ])r()('('<.tall'~ rv~'t<~/niz~d
_t);)
stt'ttlol[ ])v lllis ;:iceent;:ated loss in r e s p i r a t o r y l't~neli~+n w i t h ti~tt<,, :~fter th:lwitva..l:'at'alM o1:,s("t'V;lliOllS
OI1 tilt t'/l.~t I'II('I:llI'GI! r(,v(++llod at"t?(,llltlatt+d
d':~t]m/c + to lnitot,ltondria, n~tclei, iln~Is inet~l(led s,,'v(qlitt¢, ~li,,:order, a n d fr+igtnet~tatiott of erist:te, as well +is al~l)arent for = tn:tliolt of "attlc>fdpl&ie.. ., vesM<.'s" wit:h 'issoei"tted ] ",'S(
15(1111 t',~ ,
,,','ill I~(. rt,l.)rtt'.l.
"l't..s,' t'~,sltlts i n d i c a i e l h a r l're~'zin~ ;liq(l th;iwitl~ u m l o r Iti,zlt l)t'~:,s,.-:t~t',~, ~d'R,rs l.,(.il<,r von~litions ((.,r tlll +' ( ' r \ < + l J r i ' , < ( ' r v : [ t i o t l o f ]+,:J(111('ys t l m n i}io,-:t, ,.,.\i,:titt~ tttl
l~rO.~sttt'( '.
64, l:re,~,ze-Tha'w-lnduced Latent Injury in (]+.11~ o f t h e I:{enal C o r l t : x . +, ,1, 1'(, SI+Et<.xI>..\" I
')
,
!.;.:+t~-,:,, ><+lit,}+ <}f 5te
6 5 . R a p i d T h a w i n g o f l:r{¢zen C a n i n e K i d . nevs by MierqnvavesY f{.~I.Pl[ ]-L~rII.'rox, I,',~J,:I>l,: t-~I <.'I+. I ), ] ( ,.:TT},: Jl l,:l~, × ]I ..~Z l:: L I. t I o L ~'r, :, x I~ H / . : l l x t m x I~,. I.,1.:I, l~ (! l a r r i ~ m l l)el.mrt men t: ~,t" S~lrgival I>,e.~earell, S e l m o l uf .kle(liein(', [h~iv~'r.4tv of l'mlll.%'l','ani;t, F'hil:~Mlflli:~, l " e n l > ...,ylv:i~i:t, 1!J 10-I ). 'f'l,,rtn~Jl ~.,m~lt~,tiot't '+ech,.~i~.lues , t~s+,~l stwe,,sst':flly t'~t" tlm',viu~ t't'~z~,~ ~i
r'~ti,~, l']l(,~,trie:~l e u r r e ~ i s , imh~e(,d in :~ frozen or~a~t td;tet"~l itt an t,h,e.tri('al tMcl, g~,ne.r:rtt.; Iwa'+. intet'tmlI'c :ttiti l,('rnti+ t':ipi(l t h : t w . i ~ . A mJ+er~w;tv<, tevl~t~i~ltte w:l,~ m ' a l ~ m i e d tt...,?ilW e'initw kidI~i,,l,,,_;7,. ,;' .t~C, l!tiiTl. "l'ltis t'el~+.,r~ lU'~'s<'nts ovi]t+,y.<. ,t,'I~'+' +l~:~+ ;,'Its i~+ "' tiss+~<~ ',,,'<,'r(,,+.*te :11:=c~cxhil.fit .l/<'t]++.,i: l:.:i~llt Callillo Mtlpa"y.s were f,,rflisetl ;iC~'t'll!!l:lii'~ I~).<.'< ill i'~lll(,titql :t['t('I" frt,(,zitlo...' :tl)(t ,.vitlt rl+ilh'+t .It.1';:- ~tirnc~tllv] sult'oxh]. ,+., ( ] ) ] I , ' q ( ) ) or t l~:~\vitl~', itl ('t~t~Imt'is+.m with +uli'{'oz,:,~ tJ:'::':~l('. l ll'.; -,.;t3'rerul in oxy+,zenatod, I d l - a d j u s l e d , l:ml-. Sli<'t,.- ,,t r+m:~l t,()rit,x. (1,7 t() 0 . £ trY+it t1~ick, ;i~le('d eh'!ct t'~:dyte .-'olutior+ for .t;5 ntit~, i lnm i+n~zen w,:'i,.,liit~ ;',5 t u -15 t~,z ',v(.rc, olit:iitii:,d i'rolll ]+.ithl(.\'+ in :~ t.ol~l ~ilie(;tw oil l:m~l~ to - 5 5 ° C at th(, t'a~e of ~+l 2-~tt~,t~ll~-,+l+t t'+.'lli:th + \\'(.t )si or .re llltifot'l+ll t llawl ll;tVrresln)il+lip=g 1.o a mea;t +lmv:e~l and <:tiotl gr:tfted to the n e c k v,'ithin. 13 t:o :';3 ~nin. (Qt~.:) ;+i'lerl Itr. l:Pcsulls: All. k i d n e y s d e v e l o p e d nmri,:t,'d s w e l l t):tta sl~mved lh:tl th(' l(~ss in Qo,_, l:eiw('011 ] t() ing a n d c.,mg(~ d a y s ollly ]+5'+ i'or IlIlfI'OZPII <"()lltFoIs ulld l()<~'., ]0%, atl<;1 ](.. Histol(miealh" t.11ere ,;,;:is ttlJtlLil (]offellor;;tt.JoIi, 4::;¢;, . 41 "..,,~, a n d 5SG: fro' it(' " )zt.n" '" : n(]. th:twed liSstie. ];'reeze-iht+w-in+ht<.ed ]:.11(.!111, i n j t l r y Yeas don:ton- ~lomet'uhir swollin~ a n d vaseutar emlg(:stion. C i r c t t l a t o r v stasis ,,,.':is seen on an a r t e r t o g r a m a t ) :+', o= d a \ ' 8 . ][ai(luil:]et:tl;ion rmerosi,..+, develol,ed it+ 4 to J+'++'Sttpt+ortcd lrv G M (36418 front tlm U. S. Pttblie Healt h Servi( 6+ o4 d a y s (mean 8 d+~ys). Con~t'ol ki<:hw,,+s, per-
~:+i,li:-l..,l :,,< :~ Id+~,t.~t~,t.~mt~ it~ cr.v,:d>it,li~'.v ,,n tile l,-t,-i,. ,,t r,'>l~i.t"tliot~, i+rr~ue:~l+ilit'.', a~ttl t'o1+r+,
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fuse(l and inlphtriie{i w i t h m l l f1"e{,zill~, did SlU'\'ire. ("onrh~si{./: Dillicliliies ill coii l'1'(fllin~ i li(,rlll;il r l l l i : / w a v ; I ) o U l l ( [ a r y off('cls, :ti]d l m l i ) e r (hll'81{t~l~ of l}ow(,r, t } r e v e l ] t n i'tlll o v l l l l i : t i i o l i {:iP i h i s illi{,l't)-
way(, i(:,(,hni{tli(% 81lh{}ligh i':il)i(t soli(I t.IF.[£flll \V:I.~ ::ic{'(]n~l}lish~,(l.
lh:iwin~
66. The l/iochenli~lry of Freezing itl K i d n t w ~ . ' " ' \VII.,1.1.~,M .XI..,.\tIBttT'l:
(}f :l
In.jury ('l'issll("
B:lllk,
( ' l l n l ( ' 'ti] .Ili'c(',~li~a 1iOil I ) ( ' i } : i r l l l l ( ' l i l , .Xaval .Xl('dic:ll ]{c:,'earr.h l i ] s l i . l l i l o , N : l t i { t l i : i l N : i \'al .Xlefih::ll (.'t,ltl(,l', l~('lh{'s~la, .Xlitrvl:l;lti
20(} 14 }. }(:is llt"{'!] ~h'v('l(ll~{'(t w}l]rll • ~-~"-'~"-~ !he lfllysinJ{Igi{':ll :Jill[ l)h~'l'henlie:tl SIItlllS A rl]elll{}(l
(if :lr/:i]ysis
o.f kitlii{,vs :iflc>r v:iriolis 1)reserv:ilic)rl ('xt~'ri:rill'Ills ali(t I'if,' I(niilies (lie i n j i i r y of l}rrsi,r'c'iii(~t~ in :l lli_iriiori('nI \\'av. l lat}tfii reil.'.ll ('orlic':il slit{,:. r{':/{.'l{,,:l ill ~i -i-:'~,"=(" \ V : l r l ) ! l r ~ 1}l(~rlli()tl;itll ]l{,rforlii vilal i'liilc, li(;ils ~il(,h ~is r~,sl)ii':~li(}ii ;ll}~t i l i t } u l : i r lI',iliSllorl ill murli ~ili ~~. P/tr(~ (q1\!.1"~}i1]11("1}1 l~l :.i. ii]oasur'i[}lo oXtOl/t. ];~l.izviil(' r{,h,:i:-c. ili:i\" l_:O (t{.!t{ ~(~.t in lhe ilil"lit):ilin_ tllli,t :is w,,lt
:is end
1)ro(hl(!ts
of (q-ioi'~v. i~]et:ttn}ti.-In.
Stwli
{tll:il]lilal:d(, l)araril{~iers r(",'ll'l in a r e l } r { , l ~ I r i l r \v:iv lo s:l:lrid:lrdized iri{'r{':i.,~ii~,z ilij~lr.v. A {lif'f~,ring l')rofil{' (}f resI,.Ons{~ is'sc,(,i~ "iflt'P tl:illtzi~{' i'l'()lll l" " X" * t IGl~ .1t10. hl ~il -r-o~ (:,• fi'oili l h e h i ( t ~ i r l m n of tlyt~{~-
lh{,rmki (-I-,-5°C
io + o _., fr()lil lrvl:)Ot]]ernlir t~(~1"l'tisMli isni.(mic crvstath)i(t oxy~'(,ll:il(,
sni(," 0.5 r,e p e r 7
lior nlill flow), of +l-.. ,,11:- l'(q)orlj
an(l i r o n i ir{,ezfreezin<~ iiljtl.v.v,
ilig. "Ti':le sltbje(,l n]av., t')e (,\-,,]lu,lte(t, I-iy Ot:lservh~, sev(,r:.l] ~)f ~tt(, I}:'t l';tllli.)l-{' I'S.'. w i t h i n this svs I (,111 . A di.P.,I"llllllld ', O1''.\ (>onil)gtri.,7on has; i)(,{,ll l:l]:i{l( ~ to .~.ho(v lh{:, }~ei](:,fil (}f
(.'.ryoproiective
agen(s. Tile Iriel;}io(ts iihislrlll(,
a
w:lv Io elll01d,t o i]1o o p l l l l l l ! l l ' l lll(.,l]l{)(i{)I(}~v (Sill2}( :is ( . r y o p } l y l a e l i e (.(}11('>PlTItl,tliOll,'" fre{>Zi.ll~ raic".-01(%1- for the froozo t l r e s e r v ' i l i o l l of ii~ia(>i rt'lli~l [ 1.]S;711{;.
67. Exl}el:ililelllal and Clini('al Organ Preservation: Present Status and Future ~"
I;'rorri tl-io Bureau
of M,-di,.irle
large. T]ie. e x p e r i m e n L s ilccordirrg
• ]()IIN
]',tsi.'(I
..\. I El.., IsTI,21(, "~ ]t~llll..l¢ 1 ..{
il}i,:zi'l
I']I(.~EK.
'':
'~
I{{)X-
..tl.I) II. l)ll,:'iZM..~,\, :+ AND ]~l{'ll.\lll} {'. ],li.l.i.:ili.;l {1)('ll:trlilil'lit (}1" S l i r T l T y . 17nivrrsi~\" ~ll' .XlililieSl:tia .Xlt'~li(':tl ('Olll~'r, .Xlinll~,:ilnltis, .Xiiilli{'si )1 ,'1.) l.!lilil r c ( : ( ' l i l l \ tlVl){lllIPi-llli:i li,is t,{'t,ii !ill' llrii:,'i t}:lt Ill(Mils li) I~r~'srrv~ '. Ol'O"ill:-t. ,, ali{t li-~w,ie.~ .... ~h,-
l}riv~'(I c~i" l t . , i r '.,lsr~ll:tr s~ll~tfly. 'Ill l!!ll;g \re t'mlrl(l tIt:t{ llvt}rrt~:~ri(. (~xy..,,'~,lt roitil~im.~l ~vi:~>r'..ristiPallv w i l h }iyl){tlllt, rl/iiii t{i l,et'l~lii i, ~',:/r,, l~ros.l'vqli~}n (}t' u~iliilir, (ll',,.£;ill. ~ w i l t l ( ) l l i :i cir{.,~iJ:ll l n l i f o r 23t hi':-'. {)xy,.2'cii lili~tt,r lli'u~s~lrr l.~r~d):ll.q\ exl,I'Is l~i'~/l{,rli.\-e l~t,li(,fi~s 1}\" viri~l{, {)i I i ,{liPi.l dit'i'~isi~)lt ii]~ :t., ~}t'7:ill, :lltlt ',_}} lilt, lit'l,s{,I]{'i., ill t}r,~'sslit'+, ~il()lil,. "l'tl<, ]:iitt,r <.fft,<.t is :issttitl{,tl t,t lt<{ w i t h hYl;{i:h(,rl+lii:t :lls{i It:t> :l .-:tlli~:trv et]'~,,i ill "2Lt--tir <+r~,.'tli l+rf':<'rv:iil(tll. Slill. t+<,W+li+l U-I tit',-. ~,x\,.~, ,i.'. is stii~t,ri~+r t,i ~+is: +, -~li(,}i :~+, liitt'(~,,,i:t \Vitfi +ipIt" <+~'".i l','St' "'lty t u~i h+q'niir-.li ~ 1,,rt P+ r i o t iri,,-~,r v:~.l ivt + tltolJi(+
i}l(,rt,
i:.
i{,ltlt-}r:ir,¢
t;,ll'
si<~i~ii~t,:+lil
"l'illirlit)ll:l]" ()l'~:lllI<+,.I ,)r i,l(i,.1 :qliisllllll{',; l",llWttl~li;li t)l'ff:lJl ])l'l,.-:f'i'X'+ll t()11. :till] I t.: i1111 i:tt I is~:ll{, {l<;tll:i,.!,.t, tn('iirre{i t,v c+.',M .'iii(l ti\-l~t,rlt:iri:t nhtil~, +s ~i~,:li]\<]er+l'f,:isc+~t. Si,illi~,: witll C:lliili(' t,:i~til'..'\-, liVt'l>: :lli(t ]l:litrrt'tt,'-t',," ;itfl'.-,i ](J t l i r x':+tlit + 4~t' ]{iw th,~ (}r~lii! lY.'l'lllSlt}lt. Still, SilC("+'ssl'ilt .-h+ti+t+lt'l'lii ,-l<+i'':l~t ~ inethotls, alit;ou~]l <'iinirall\' llsf'iiif :ii l)l"{,..-a+ o111, ,'lri, }+11t, lC,lli]+.()r:trv .gtlJil;< till lilt' / l l l l l i l : t l P 7p+lI I() l'r(>OZlti~ i)I'{£ctlls l(i ~}le l('iil[iPralt+r{ ~ {~f liqtli~l ilil;l'l}ffo]l f<}r in,.hqiliitv, viallh: , st,.,r:tp.;~, lit b:;ii+ks, t](!rt' a~:iin, liyl}oih(,rlnia, tlvl)("rtrlr{:l. Plld i,ijl] I inuotlS 1 ~et'filsi(m :t re usel'tit in t~l'(,t );i r:l l i o n of il]e (it'd;ill for ft'eezili.<.£ t.,i.-: wull :~., for tI'ai]Sl)l;il+i t}retmraiiotl allot lll:t\vhi~t, l]ol+eftltty, this :il}tii'+),iPli, e,.ltiliiillcd witil nc\v fi'~,t,z{ +l.haw i n ( , r h o t l & co~illI e i ; h r l i i l a t e in t'e:i{lv :lv allabilit\ of 1)reserv{ +l, frozoi-t o r g a n s ,,7.'ilil+il)l(, for l+railspiaiittlliOil tlroootliire8 i;tl ttu.~<'i:iliS w i i l i i n i ho, lleXt 9 {lo(,+idps,
and Surgery,
~N"nv;v .Depart:lllont;, lles(::itrch T a s k .\'o; M43(iDJ,)3o004. TIlie ol~inions or a.ssertions Ponl.airii?d herein are the t-n'ivate ones of l.h(> lill't.hoP and lil'O I1O1. t o ])i) ('('Jl'lS{l'tlO(t B.S Official or refle(:tiilg t h0 .views o f " t,), ," . ~ ", or tile Naval ~ervl(,e " "_ at l:tl(, N a v y l)ctaitnl(..nl,
d.ucied
ll(,lleS. \ V I I . i , I A M ( l . . X I A X X X ,
r e p o r l e d her(qll were co.ilt;o ttlo ln'iii('il)lt, enurtclat.ed .in
Guide Ior L a b o r a t o r y Animal Facilities ,.~n •t c..,ar?.
68. Temlmrary Suspension of Animaihm in l ) o g s b y M e a n s o f R e f r i g e r a t e d Asan,.r a t i n e P e r f u s i o n . (',.~l.;l~AM} I(I£E'BAN-OFF ( Aer(> s.t}a('e N[edi('al L a b o r a t o r y t {..,131]l(.al }, Wili'ord Hall I ; S A F H.OSlfital, ,[=l('kMnd Air tr(}r(.{. bast., T e x a s ) at an{hlr{liz(.g:t ilionvrel dogs ~)f intm_~ i]lnll :20 kg •
[-i
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k.itlfl('Vs
,+lll;tSiOltl(ist,tl
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+';Wl~rt,:illv f(~t" 6 hrs i~tdie:l.!es ri,t~,tltiml of hiot,:..zie:~l ['tttwth>tls in ~-:ev('t':il c:tst's' v,'llMt was :~ls<:, ih,'
(~l>set'v-~tiolt
I(]fl~-l{'rlll
of
:-( ,vt't,t[" ~tni~tt:'lflt-
~h),..+s ',xilh tlw reimfd:~ntt,~t k M t w v :tl'T~+I" l l'(h'iIlllt'll|, llllt]f'l' high l~r('s~ll'C ])r()('('<.tall'~ rv~'t<~/niz~d
_t);)
stt'ttlol[ ])v lllis ;:iceent;:ated loss in r e s p i r a t o r y l't~neli~+n w i t h ti~tt<,, :~fter th:lwitva..l:'at'alM o1:,s("t'V;lliOllS
OI1 tilt t'/l.~t I'II('I:llI'GI! r(,v(++llod at"t?(,llltlatt+d
d':~t]m/c + to lnitot,ltondria, n~tclei, iln
15(1111 t',~ ,
,,','ill I~(. rt,l.)rtt'.l.
"l't..s,' t'~,sltlts i n d i c a i e l h a r l're~'zin~ ;liq(l th;iwitl~ u m l o r Iti,zlt l)t'~:,s,.-:t~t',~, ~d'R,rs l.,(.il<,r von~litions ((.,r tlll +' ( ' r \ < + l J r i ' , < ( ' r v : [ t i o t l o f ]+,:J(111('ys t l m n i}io,-:t, ,.,.\i,:titt~ tttl
l~rO.~sttt'( '.
64, l:re,~,ze-Tha'w-lnduced Latent Injury in (]+.11~ o f t h e I:{enal C o r l t : x . +, ,1, 1'(, SI+Et<.xI>..\" I
')
,
!.;.:+t~-,:,, ><+lit,}+ <}f 5te
6 5 . R a p i d T h a w i n g o f l:r{¢zen C a n i n e K i d . nevs by MierqnvavesY f{.~I.Pl[ ]-L~rII.'rox, I,',~J,:I>l,: t-~I <.'I+. I ), ] ( ,.:TT},: Jl l,:l~, × ]I ..~Z l:: L I. t I o L ~'r, :, x I~ H / . : l l x t m x I~,. I.,1.:I, l~ (! l a r r i ~ m l l)el.mrt men t: ~,t" S~lrgival I>,e.~earell, S e l m o l uf .kle(liein(', [h~iv~'r.4tv of l'mlll.%'l','ani;t, F'hil:~Mlflli:~, l " e n l > ...,ylv:i~i:t, 1!J 10-I ). 'f'l,,rtn~Jl ~.,m~lt~,tiot't '+ech,.~i~.lues , t~s+,~l stwe,,sst':flly t'~t" tlm',viu~ t't'~z~,~ ~i
r'~ti,~, l']l(,~,trie:~l e u r r e ~ i s , imh~e(,d in :~ frozen or~a~t td;tet"~l itt an t,h,e.tri('al tMcl, g~,ne.r:rtt.; Iwa'+. intet'tmlI'c :ttiti l,('rnti+ t':ipi(l t h : t w . i ~ . A mJ+er~w;tv<, tevl~t~i~ltte w:l,~ m ' a l ~ m i e d tt...,?ilW e'initw kidI~i,,l,,,_;7,. ,;' .t~C, l!tiiTl. "l'ltis t'el~+.,r~ lU'~'s<'nts ovi]t+,y.<. ,t,'I~'+' +l~:~+ ;,'Its i~+ "' tiss+~<~ ',,,'<,'r(,,+.*te :11:=c~cxhil.fit .l/<'t]++.,i: l:.:i~llt Callillo Mtlpa"y.s were f,,rflisetl ;iC~'t'll!!l:lii'~ I~).<.'< ill i'~lll(,titql :t['t('I" frt,(,zitlo...' :tl)(t ,.vitlt rl+ilh'+t .It.1';:- ~tirnc~tllv] sult'oxh]. ,+., ( ] ) ] I , ' q ( ) ) or t l~:~\vitl~', itl ('t~t~Imt'is+.m with +uli'{'oz,:,~ tJ:'::':~l('. l ll'.; -,.;t3'rerul in oxy+,zenatod, I d l - a d j u s l e d , l:ml-. Sli<'t,.- ,,t r+m:~l t,()rit,x. (1,7 t() 0 . £ trY+it t1~ick, ;i~le('d eh'!ct t'~:dyte .-'olutior+ for .t;5 ntit~, i lnm i+n~zen w,:'i,.,liit~ ;',5 t u -15 t~,z ',v(.rc, olit:iitii:,d i'rolll ]+.ithl(.\'+ in :~ t.ol~l ~ilie(;tw oil l:m~l~ to - 5 5 ° C at th(, t'a~e of ~+l 2-~tt~,t~ll~-,+l+t t'+.'lli:th + \\'(.t )si or .
~:+i,li:-l..,l :,,< :~ Id+~,t.~t~,t.~mt~ it~ cr.v,:d>it,li~'.v ,,n tile l,-t,-i,. ,,t r,'>l~i.t"tliot~, i+rr~ue:~l+ilit'.', a~ttl t'o1+r+,
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A N NU.:\ i, M ICI'2'I'I N G A BST !i A(2"I'S
fuse(l and inlphtriie{i w i t h m l l f1"e{,zill~, did SlU'\'ire. ("onrh~si{./: Dillicliliies ill coii l'1'(fllin~ i li(,rlll;il r l l l i : / w a v ; I ) o U l l ( [ a r y off('cls, :ti]d l m l i ) e r (hll'81{t~l~ of l}ow(,r, t } r e v e l ] t n i'tlll o v l l l l i : t i i o l i {:iP i h i s illi{,l't)-
way(, i(:,(,hni{tli(% 81lh{}ligh i':il)i(t soli(I t.IF.[£flll \V:I.~ ::ic{'(]n~l}lish~,(l.
lh:iwin~
66. The l/iochenli~lry of Freezing itl K i d n t w ~ . ' " ' \VII.,1.1.~,M .XI..,.\tIBttT'l:
(}f :l
In.jury ('l'issll("
B:lllk,
( ' l l n l ( ' 'ti] .Ili'c(',~li~a 1iOil I ) ( ' i } : i r l l l l ( ' l i l , .Xaval .Xl('dic:ll ]{c:,'earr.h l i ] s l i . l l i l o , N : l t i { t l i : i l N : i \'al .Xlefih::ll (.'t,ltl(,l', l~('lh{'s~la, .Xlitrvl:l;lti
20(} 14 }. }(:is llt"{'!] ~h'v('l(ll~{'(t w}l]rll • ~-~"-'~"-~ !he lfllysinJ{Igi{':ll :Jill[ l)h~'l'henlie:tl SIItlllS A rl]elll{}(l
(if :lr/:i]ysis
o.f kitlii{,vs :iflc>r v:iriolis 1)reserv:ilic)rl ('xt~'ri:rill'Ills ali(t I'if,' I(niilies (lie i n j i i r y of l}rrsi,r'c'iii(~t~ in :l lli_iriiori('nI \\'av. l lat}tfii reil.'.ll ('orlic':il slit{,:. r{':/{.'l{,,:l ill ~i -i-:'~,"=(" \ V : l r l ) ! l r ~ 1}l(~rlli()tl;itll ]l{,rforlii vilal i'liilc, li(;ils ~il(,h ~is r~,sl)ii':~li(}ii ;ll}~t i l i t } u l : i r lI',iliSllorl ill murli ~ili ~~. P/tr(~ (q1\!.1"~}i1]11("1}1 l~l :.i. ii]oasur'i[}lo oXtOl/t. ];~l.izviil(' r{,h,:i:-c. ili:i\" l_:O (t{.!t{ ~(~.t in lhe ilil"lit):ilin_ tllli,t :is w,,lt
:is end
1)ro(hl(!ts
of (q-ioi'~v. i~]et:ttn}ti.-In.
Stwli
{tll:il]lilal:d(, l)araril{~iers r(",'ll'l in a r e l } r { , l ~ I r i l r \v:iv lo s:l:lrid:lrdized iri{'r{':i.,~ii~,z ilij~lr.v. A {lif'f~,ring l')rofil{' (}f resI,.Ons{~ is'sc,(,i~ "iflt'P tl:illtzi~{' i'l'()lll l" " X" * t IGl~ .1t10. hl ~il -r-o~ (:,• fi'oili l h e h i ( t ~ i r l m n of tlyt~{~-
lh{,rmki (-I-,-5°C
io + o _., fr()lil lrvl:)Ot]]ernlir t~(~1"l'tisMli isni.(mic crvstath)i(t oxy~'(,ll:il(,
sni(," 0.5 r,e p e r 7
lior nlill flow), of +l-.. ,,11:- l'(q)orlj
an(l i r o n i ir{,ezfreezin<~ iiljtl.v.v,
ilig. "Ti':le sltbje(,l n]av., t')e (,\-,,]lu,lte(t, I-iy Ot:lservh~, sev(,r:.l] ~)f ~tt(, I}:'t l';tllli.)l-{' I'S.'. w i t h i n this svs I (,111 . A di.P.,I"llllllld ', O1''.\ (>onil)gtri.,7on has; i)(,{,ll l:l]:i{l( ~ to .~.ho(v lh{:, }~ei](:,fil (}f
(.'.ryoproiective
agen(s. Tile Iriel;}io(ts iihislrlll(,
a
w:lv Io elll01d,t o i]1o o p l l l l l l ! l l ' l lll(.,l]l{)(i{)I(}~v (Sill2}( :is ( . r y o p } l y l a e l i e (.(}11('>PlTItl,tliOll,'" fre{>Zi.ll~ raic".-01(%1- for the froozo t l r e s e r v ' i l i o l l of ii~ia(>i rt'lli~l [ 1.]S;711{;.
67. Exl}el:ililelllal and Clini('al Organ Preservation: Present Status and Future ~"
I;'rorri tl-io Bureau
of M,-di,.irle
large. T]ie. e x p e r i m e n L s ilccordirrg
• ]()IIN
]',tsi.'(I
..\. I El.., IsTI,21(, "~ ]t~llll..l¢ 1 ..{
il}i,:zi'l
I']I(.~EK.
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'~
I{{)X-
..tl.I) II. l)ll,:'iZM..~,\, :+ AND ]~l{'ll.\lll} {'. ],li.l.i.:ili.;l {1)('ll:trlilil'lit (}1" S l i r T l T y . 17nivrrsi~\" ~ll' .XlililieSl:tia .Xlt'~li(':tl ('Olll~'r, .Xlinll~,:ilnltis, .Xiiilli{'si )1 ,'1.) l.!lilil r c ( : ( ' l i l l \ tlVl){lllIPi-llli:i li,is t,{'t,ii !ill' llrii:,'i t}:lt Ill(Mils li) I~r~'srrv~ '. Ol'O"ill:-t. ,, ali{t li-~w,ie.~ .... ~h,-
l}riv~'(I c~i" l t . , i r '.,lsr~ll:tr s~ll~tfly. 'Ill l!!ll;g \re t'mlrl(l tIt:t{ llvt}rrt~:~ri(. (~xy..,,'~,lt roitil~im.~l ~vi:~>r'..ristiPallv w i l h }iyl){tlllt, rl/iiii t{i l,et'l~lii i, ~',:/r,, l~ros.l'vqli~}n (}t' u~iliilir, (ll',,.£;ill. ~ w i l t l ( ) l l i :i cir{.,~iJ:ll l n l i f o r 23t hi':-'. {)xy,.2'cii lili~tt,r lli'u~s~lrr l.~r~d):ll.q\ exl,I'Is l~i'~/l{,rli.\-e l~t,li(,fi~s 1}\" viri~l{, {)i I i ,{liPi.l dit'i'~isi~)lt ii]~ :t., ~}t'7:ill, :lltlt ',_}} lilt, lit'l,s{,I]{'i., ill t}r,~'sslit'+, ~il()lil,. "l'tl<, ]:iitt,r <.fft,<.t is :issttitl{,tl t,t lt
i}l(,rt,
i:.
i{,ltlt-}r:ir,¢
t;,ll'
si<~i~ii~t,:+lil
"l'illirlit)ll:l]" ()l'~:lll
and Surgery,
~N"nv;v .Depart:lllont;, lles(::itrch T a s k .\'o; M43(iDJ,)3o004. TIlie ol~inions or a.ssertions Ponl.airii?d herein are the t-n'ivate ones of l.h(> lill't.hoP and lil'O I1O1. t o ])i) ('('Jl'lS{l'tlO(t B.S Official or refle(:tiilg t h0 .views o f " t,), ," . ~ ", or tile Naval ~ervl(,e " "_ at l:tl(, N a v y l)ctaitnl(..nl,
d.ucied
ll(,lleS. \ V I I . i , I A M ( l . . X I A X X X ,
r e p o r l e d her(qll were co.ilt;o ttlo ln'iii('il)lt, enurtclat.ed .in
Guide Ior L a b o r a t o r y Animal Facilities ,.~n •t c..,ar?.
68. Temlmrary Suspension of Animaihm in l ) o g s b y M e a n s o f R e f r i g e r a t e d Asan,.r a t i n e P e r f u s i o n . (',.~l.;l~AM} I(I£E'BAN-OFF ( Aer(> s.t}a('e N[edi('al L a b o r a t o r y t {..,131]l(.al }, Wili'ord Hall I ; S A F H.OSlfital, ,[=l('kMnd Air tr(}r(.{. bast., T e x a s ) at an{hlr{liz(.g:t ilionvrel dogs ~)f intm_~ i]lnll :20 kg •
[-i
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